scholarly journals Evaluating the Diversity of Ecotypes of Red Clover (Trifolium pratense L.) from Northwestern Spain by Phenotypic Traits and Microsatellites

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2275
Author(s):  
Cristina Isabel Fernández-Otero ◽  
Ana María Ramos-Cabrer ◽  
Julio Enrique López-Díaz ◽  
Santiago Pereira-Lorenzo
Keyword(s):  
Trifolium Pratense ◽  
Agricultural Research ◽  
Genotypic Variation ◽  
Natural Populations ◽  
Red Clover ◽  
Research Centre ◽  
Phenotypic Traits ◽  
Grassland Species ◽  
Ssr Loci ◽  
Northwestern Spain

For more than 50 years, the CIAM-AGACAL (Agricultural Research Centre of Mabegondo, Xunta de Galicia, A Coruña, Spain) has been carrying out the important task of conserving the phytogenetic resources of ecotypes and natural populations of grassland species from northwestern Spain. The CIAM-AGACAL’s germplasm bank has 57 populations of red clover (Trifolium pratense Lam.), one of the most cultivated forage legumes in the world. The goal of the present study was to evaluate the diversity among cultivars and natural clover populations at morphological and molecular level. Twelve polymorphic SSR loci revealed 241 microsatellite alleles with an average of 20.08 alleles per locus. Two main groups were detected by the Structure software, one of them including local populations and the second clustering cultivars and related populations. Intra-specific variability was found among cultivars and natural populations. A moderate genetic differentiation of Spanish red clover cultivars was observed (Fst = 0.08) between the two main clusters. Finally, a certain relationship between phenotypic and genotypic variation was detected.

Tapani red clover

2008 ◽  
Vol 88 (6) ◽  
pp. 1091-1092 ◽  
Author(s):  
Y. A. Papadopoulos ◽  
B. R. Christie ◽  
T. M. Choo ◽  
R. Michaud ◽  
K. B. McRae ◽  
...  
Keyword(s):  
Prince Edward Island ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Phenotypic Selection ◽  
Growth Potential ◽  
Research Centre ◽  
Quebec City ◽  
Original Material ◽  
Trifolium Pratense L ◽  
Cultivar Description

Tapani is a 21-clone diploid synthetic cultivar of red clover (Trifolium pratense L.). It was developed by phenotypic selection at Agriculture and Agri-Food Canada, Crops and Livestock Research Centre, in Charlottetown, Prince Edward Island, and at the Soils and Crops Research and Development Centre in Quebec City, Quebec. The original material for this strain was selected from collections made in old stands of red clover in three Atlantic Provinces of Canada. Tapani is early flowering and winterhardy. In Atlantic Canada, Tapani yielded an average of 109% of the check cultivar Marino over three production years. This cultivar has superior re-growth potential with high second-cut herbage yield. Key words: Red clover, Trifolium pratense L., cultivar description

scholarly journals The persistency evaluation of Czech varieties of Red (Trifolium pratense L.) and Alsike Clover (Trifolium hybridum L.)

2006 ◽  
Vol 54 (4) ◽  
pp. 133-138 ◽  
Author(s):  
Stanislav Hejduk
Keyword(s):  
Czech Republic ◽  
Trifolium Pratense ◽  
Arable Land ◽  
Forage Quality ◽  
Red Clover ◽  
Soil Condition ◽  
Forage Legume ◽  
The Czech Republic ◽  
Grassland Species ◽  
Trifolium Pratense L

Red clover is the most important forage legume in the Czech Republic. It is an unassuming species to climatic and soil condition, is suitable for mixtures with grasses and it is above other grassland species in forage quality. The most serious problem of red clover in grasslands is its lack of persistency (2–4 years). Considering of red clover growing area on arable land decrease and area of permanent grasslands increases, becomes persistency of varieties important attribute. The persistency of all Czech varieties was evaluated on the basis of dominance proportion investigation after 3 winters. Assessed varieties were established in mixture with grasses (60:40%). Significantly most persistent varieties were Amos 4n (24.5%), Dolina 4n (20.25 %) and Radegast 4n (19.25%). Varieties of Alsike clover were not significantly different from varieties of red clover.

scholarly journals Efficiency of SSR and PawS markers for evaluation of genetic polymorphism among red clover (Trifolium pratense L.) cultivars

2020 ◽  
Vol 181 (3) ◽  
pp. 100-109
Author(s):  
I. A. Klimenko ◽  
S. I. Kostenko ◽  
Yu. M. Mavlyutov ◽  
A. O. Shamustakimova
Keyword(s):  
Dna Polymorphism ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Crop Varieties ◽  
Pcr Products ◽  
Ssr Loci ◽  
Trifolium Pratense L ◽  
The Mean ◽  
Unique Amplicons ◽  
Dna Profiles

Background. Identification of crop varieties is presently one of the most important aspects due a significant annual increase in the number of newly developed cultivars. Application of molecular markers makes it possible to identify cultivars and secure protection of plant breeders’ rights. Marker techniques based on SSR loci and PawS markers were evaluated for their efficiency in revealing the DNA polymorphism in Russian red clover cultivars, and the research results are presented in this publication.Materials and Methods. The total genome DNA was extracted by a modified SDS method from 30 seedlings per each cultivar. Nine simple sequence repeats (SSR) and 4 PawS markers were used for genotyping. The basic genetic diversity parameters were measured and analyzed using the software resources GelAnalyzer 2010а, MStools v.3, and Statistica 7.0.Results and conclusion. The mean level of intervarietal DNA polymorphism in red clover was 38.6%. Cultivar-specific amplicons were obtained for 4 accessions (cvs. ‘Trifon’, ‘Topaz’, ‘Trio’ and ‘Mars’) with SSR loci RCS1307 and RCS3095. These loci were found appropriate for identification and certification of such cultivars. The tested PawS markers (individually and in combinations) proved non-informative for the analysis of intervarietal DNA polymorphism in red clover. The only primer pair PawS5+PawS16 generated reproducible PCR products, but unique amplicons were absent in the DNA profiles. The data obtained in this study may be helpful for further identification and certification of Russian red clover cultivars and promising breeding materials. 

scholarly journals The estimated nutritive value of three common grassland species at three primary growth harvest dates following ensiling and fractionation of press-cake

2013 ◽  
Vol 22 (1) ◽  
pp. 194-200 ◽  
Author(s):  
Joseph McEniry ◽  
Padraig O'Kiely
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Trifolium Pratense ◽  
Press Cake ◽  
Substantial Reduction ◽  
Red Clover ◽  
Grassland Species ◽  
Harvest Dates ◽  
The Press ◽  
Ruminant Diets

 In a Green Biorefinery processing green biomass one possible application for the press-cake fraction is as a feedstuff for ruminants. This study investigates the effects of ensiling and fractionation on the estimated nutritive value of three grassland species harvested at different stages of maturity. Perennial ryegrass (Lolium perenne L., var. Gandalf), cocksfoot (Dactylis glomerata L., var. Pizza) and red clover (Trifolium pratense L., var. Merviot) were grown in field plots and harvested and ensiled in laboratory silos. These silages were subsequently fractionated into press-cake and press-juice fractions. Loss of soluble, fermentable organic matter during ensiling increased the relative proportions of fibre and crude protein. Fractionation resulted in the substantial reduction of herbage soluble nutrient and mineral content, increasing the fibre content and reducing digestibility and crude protein. The low energy and protein content of the press-cake fraction, especially at later harvest dates, will limit its use in ruminant diets.

scholarly journals Evaluation of Lentil Genotypes (Lens culinaris Medikus) for Growth and Yield Performances across Climate Conditions of Central Highlands of Ethiopia

2018 ◽  
Vol 6 (11) ◽  
pp. 1575
Author(s):  
Nano Alemu Daba ◽  
Eba Muluneh Sorecha
Keyword(s):  
Seed Yield ◽  
Agricultural Research ◽  
Global Climate ◽  
Block Design ◽  
Genotypic Variation ◽  
Growth And Yield ◽  
Research Centre ◽  
Cropping Season ◽  
Central Highlands ◽  
Cropping Seasons

Lentil crop has largely been affected by global climate changing, particularly, due to rainfall and temperature changing and variability. Hence, the experiment was conducted in Central Highlands of Ethiopia, namely Akaki, Chefe Donsa and Bishoftu Agricultural Research Centre, in the 2009 and 2010 cropping seasons to assess the genotypic variation of lentil for growth and yield performances. Five lentil genotypes viz. 87S-93549 X EL-103-16, 87S-93549 X EL-103-9-1, Alemaya X FLIP 88-41L-02-AK-14, Alemaya X FLIP 88-41L-02-AK-6 were arranged in randomized complete block design with four replications at all locations. The result of study revealed that the genotype 87S-93549 X EL-103-9-1 produced the highest seed yield of 1985.90 and 2500.00 kg ha-1 during 2009 and 2010 cropping seasons, respectively, at Akaki. On the other hand, the genotype Alemaya X FLIP 88-41L-02-AK-14 gave the highest seed yield (4189.1 kg ha-1) at Chefe Donsa in 2009 cropping season. The genotype X FLIP 88-41L-02-AK-14 was found to be the earliest in physiological maturity, accounting 128.75 days, and the genotype 87S-93549 X EL-103-9-1 had yield advantage of 27.6% over the local check at Chefe Donsa in the 2010 cropping season. Generally, all lentil genotypes were performed better at Chefe Donsa, followed by at Akaki and Bishoftu locations during both cropping seasons. Rainfall and temperature conditions have enormous influences on the growth and yields performances of lentil genotypes at all research locations. The study inspires other studies to be conducted on other factors than climate events thwarting the production of lentil crops in the study areas.

AC Endure red clover

1998 ◽  
Vol 78 (1) ◽  
pp. 113-114 ◽  
Author(s):  
B. R. Christie ◽  
T. M. Choo ◽  
Y. A. Papadopoulos ◽  
J. Lewis ◽  
R. Michaud
Keyword(s):  
Key Words ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Forage Yield ◽  
Research Centre ◽  
Mass Selection ◽  
Trifolium Pratense L ◽  
Cultivar Description ◽  
Diploid Cultivar

AC Endure red clover (Trifolium pratense L.) is a diploid cultivar of the double-cut type developed by mass selection at the Crop and Livestock Research Centre, Agriculture and Agri-Food Canada, Charlottetown, PEI. It is earlier flowering than Florex or Marino, and higher in forage yield, especially in the second and third harvest years. Key words: Red clover, Trifolium pratense L., cultivar description

AC Christie red clover

1999 ◽  
Vol 79 (2) ◽  
pp. 257-258 ◽  
Author(s):  
R. A. Martin ◽  
B. R. Christie ◽  
Y. A. Papadopoulos ◽  
R. C. Martin
Keyword(s):  
Key Words ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Phenotypic Selection ◽  
Early Flowering ◽  
Research Centre ◽  
Atlantic Canada ◽  
Trifolium Pratense L ◽  
Cultivar Description ◽  
Diploid Cultivar

AC Christie red clover (Trifolium pratense L.) is a diploid cultivar of the double-cut type. It was developed by phenotypic selection at the Crop and Livestock Research Centre, Agriculture and Agri-Food Canada, Charlottetown, PEI. It is early flowering, very winterhardy and has no pubescence on the stems. In Atlantic Canada it has produced more forage than the check cultivars in the second and third harvest years. Key words: Red clover, Trifolium pratense, cultivar description

scholarly journals THE EVALUATION OF THE COMPATIBILITY OF CEREAL AND GREEN MANURE ON THE BASIS OF NUTRIENTS

2018 ◽  
Author(s):  
Aušra ARLAUSKIENĖ ◽  
Viktorija GECAITĖ ◽  
Danutė JABLONSKYTĖ-RAŠČĖ
Keyword(s):  
Winter Wheat ◽  
Spring Wheat ◽  
Green Manure ◽  
Trifolium Pratense ◽  
Cropping System ◽  
Red Clover ◽  
Research Centre ◽  
Similar Amount ◽  
Clay Loam ◽  
Trifolium Pratense L

Research was carried out at the Lithuanian Research Centre for Agriculture and Forestry’s (LAMMC) Joniškėlis Experimental Station on a clay loam Endocalcari Endohypogleyic Cambisol. The study was aimed to explore the aboveground mass of perennial forage legumes: red clover (Trifolium pratense L.) and lucerne (Medicago sativa L.), and their mixtures with festulolium (x Festuliolium), used as green manure, qualitative parameters and compatibility with cereals on the basis of nutrients nitrogen (N), phosphorus (P) and potassium (K). The deficiency of other nutrients (P, K) and intensity of green manure mineralization can lead to N absorption. It has been determined that winter wheat takes one kg of N together with 0.2 kg P and 0.6 kg K. Spring wheat requires a similar amount of P but a higher amount of K. Average winter wheat grain yield can be 4.0 t ha-1 on a clay loam Cambisol in organic cropping system. NPK content – 134 kg ha-1 is needed for such productivity (grain + straw). This content is lower for spring winter growing. P:N and K:N ratios are more favourable in perennial forage legume mixture with festulolium, as compared to legume alone. To obtain grain yields of 4 t ha-1 of winter wheat and 3 t ha-1 of spring wheat in balanced organic crop rotation it is sufficient to apply 3.0 and 2.0 t ha-1 DM of pure legume mass as green manure. “Cut-and-carry” fertilisers do not satisfy the wheat demand for P.

scholarly journals Allelic Variants for Candidate Nitrogen Fixation Genes Revealed by Sequencing in Red Clover (Trifolium pratense L.)

2019 ◽  
Vol 20 (21) ◽  
pp. 5470 ◽  
Author(s):  
Oldřich Trněný ◽  
David Vlk ◽  
Eliška Macková ◽  
Michaela Matoušková ◽  
Jana Řepková ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Candidate Genes ◽  
Trifolium Pratense ◽  
Genotypic Variation ◽  
Intraspecific Variability ◽  
Red Clover ◽  
Ethylene Response Factor ◽  
Allelic Variants ◽  
Alternative Phenotypes ◽  
Biological Nitrogen

Plant–rhizobia symbiosis can activate key genes involved in regulating nodulation associated with biological nitrogen fixation (BNF). Although the general molecular basis of the BNF process is frequently studied, little is known about its intraspecific variability and the characteristics of its allelic variants. This study’s main goals were to describe phenotypic and genotypic variation in the context of nitrogen fixation in red clover (Trifolium pretense L.) and identify variants in BNF candidate genes associated with BNF efficiency. Acetylene reduction assay validation was the criterion for selecting individual plants with particular BNF rates. Sequences in 86 key candidate genes were obtained by hybridization-based sequence capture target enrichment of plants with alternative phenotypes for nitrogen fixation. Two genes associated with BNF were identified: ethylene response factor required for nodule differentiation (EFD) and molybdate transporter 1 (MOT1). In addition, whole-genome population genotyping by double-digest restriction-site-associated sequencing (ddRADseq) was performed, and BNF was evaluated by the natural 15N abundance method. Polymorphisms associated with BNF and reflecting phenotype variability were identified. The genetic structure of plant accessions was not linked to BNF rate of measured plants. Knowledge of the genetic variation within BNF candidate genes and the characteristics of genetic variants will be beneficial in molecular diagnostics and breeding of red clover.

scholarly journals A Forage Mixture with Red Clover in the Fourth Experimental Year

2016 ◽  
Vol 73 (2) ◽  
pp. 349
Author(s):  
Ioan Rotar ◽  
Roxana Vidican ◽  
Florin Păcurar ◽  
Anamaria Mălinaş ◽  
Ioana Vaida ◽  
...  
Keyword(s):  
Festuca Arundinacea ◽  
Trifolium Pratense ◽  
Agricultural Research ◽  
Complex Mixture ◽  
Red Clover ◽  
Climatic Conditions ◽  
Phleum Pratense ◽  
Mineral Fertilization ◽  
Agricultural Research And Development ◽  
Different Doses

The present paper aims to follow the behaviour of a forage mixture with red clover in the fourth experimental year. We followed the behaviour of a complex mixture consisting of Trifolium pratense (15%), Lolium perenne (20%), Festulolium (25%), Festuca arundinacea (25%) and Phleum pratense (15%) under the influence of mineral fertilization in the climatic conditions specific Plateau of Transylvania, Romania. Experience was installed in the spring of 2012 in experimental fields located inside the Agricultural Research and Development Station Turda. The mixture was sown on 2 densities namely 12.5 cm and 25 cm distances between rows and they were fertilized in early March with complex NPK and ammonium nitrate (33.3%) in four different doses: V1 - control variant, unfertilized, V2-N50P60K80, V3- N75P60K80 and V4 -N100P60K80. An interesting evolution of the forage mixture was observed in the fourth experimental year. Fertilization with N75P60K80 seemed to have a favourable influence on forage mixture productivity. 

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