Biocontrol Potential and Catabolic Profile of Endophytic Diaporthe eres Strain 1420S from Prunus domestica L. in Poland—A Preliminary Study

Recently, Diaporthe has been considered the most frequently isolated genera of endophytic fungi, having a broad spectrum of host plants and a worldwide distribution. The endophytic Diaporthe strain used in the present work came from the Fungal Collection of Phytopathology and Mycology Subdepartment, University of Life Sciences in Lublin (Poland), and was isolated from healthy Prunus domestica shoots during previous studies. Due to the possibility of using the Diaporthe endophytes as a promising option for plant disease management, the main goal of the research was to study the antagonistic effect of endophytic Diaporthe strain against six phytopathogens: Verticillium dahliae, Botrytis cinerea, Fusarium avenaceum, F. sprotrichioides, Alternaria alternata, and Trichothecium roseum based on the dual culture assay and to determine the catabolic profile of the endophyte by using Biolog FF Plates. The dual-culture test assay revealed the ability of the endophytic Diaporthe to limit the growth of all tested pathogens. The growth inhibition percentage ranged from 20% (V. dahliae) to 40% (T. roseum). A distinct zone of inhibition occurred between the endophytic Diaporthe and the pathogens T. roseum, V. dahliae, and B. cinerea in the co-growth combinations. As for the catabolic profile results, the most intensive utilization of carbon substrates was observed after 168 h of incubation. The growth of the analyzed strain was observed on 79 media containing carbohydrates, carboxylic acids, amino acids, amines and amides, polymers, and others. The most effective decomposition was observed in the polymers group, the least in amines and amides. Molecular identification indicated that this strain was closely related to the Diaporthe eres species complex.

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Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

KnE Life Sciences ◽

10.18502/kls.v2i6.1051 ◽

2017 ◽

Vol 2 (6) ◽

pp. 287 ◽

Cited By ~ 1

Author(s):

Fitri Widiantini ◽

Andri Herdiansyah ◽

Endah Yulia

Keyword(s):

Endophytic Bacteria ◽

Rice Blast ◽

Biocontrol Agent ◽

Pyricularia Oryzae ◽

Rice Blast Disease ◽

Blast Disease ◽

Dual Culture ◽

Biocontrol Activity ◽

Dual Culture Assay ◽

Biocontrol Potential

Isolation was attempted to collect endophytic bacteria as potential biocontrol agents against rice blast disease (Pyricularia oryzae Cav.). The disease is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led to the searching of alternative controlling method to replace the commonly used pesticide-based method. Endophytic bacteria are bacteria that have intimate relationship with their host without inducing any pathogenic symptom. The use of endophytic microbial as biocontrol agent has its own advantages as the microbes are more easily to adapt to the environment needed by the host plant. We evaluated endophytic bacteria isolated from healthy rice plants and tested for their potential biocontrol activity using dual culture assay. Ten isolates were found to inhibit the growth of P. oryzae of more than 50%. Microscopic observation showed that they were able to cause the mycelia malformation of P. oryzae. Further work is currently in progress to determine their effectiveness in the pot trial. Keywords: Endophytic bacteria; Pyricularia oryzae; biocontrol.

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Isolation, Identification and In Vitro Test for the Biocontrol Potential of Trichoderma viride on Fusarium oxysporum f. sp. Lycopersici

The Open Agriculture Journal ◽

10.2174/1874331502115010010 ◽

2021 ◽

Vol 15 (1) ◽

pp. 10-20

Author(s):

Tsegaye Mekuria Ayele ◽

Guesh Desta Gebremariam ◽

Subban Patharajan

Keyword(s):

Fusarium Oxysporum ◽

Trichoderma Viride ◽

Dual Culture ◽

In Vitro Test ◽

Tomato Production ◽

Biocontrol Efficacy ◽

Pests And Diseases ◽

Dual Culture Assay ◽

Biocontrol Potential

Introduction: Tomato production in Ethiopia is challenged by many pests and diseases. Fusarium wilt is one of the most important diseases of tomato affecting its productivity. Methods: Tomato tissue and soil samples were collected from tomato farmlands around Aksum town to isolate and identify pathogenic Fusarium species and Trichoderma species with biocontrol efficacy. Samples were processed in the Aksum University Biotechnology laboratory following standard procedures. Results and Discussion: Eight Fusarium and five Trichoderma isolates were obtained. Six of the Fusarium isolates were identified as Fusarium oxysporum, whereas the remaining two were Fusarium equiseti and Fusarium circinatum. Detached leaf bioassay of the F. oxysporum on tomato leaves showed leaf lesion on the tomato variety, Melka oda. The isolated Trichoderma strains were screened for biocontrol potential against virulent F. oxysporum in vitro. The Trichoderma isolate showing the highest biocontrol efficacy against the virulent Fusarium was morphologically identified as Trichoderma viride. in vitro F. oxysporum-T. viride dual culture assay demonstrated that T. viride inhibits the growth of F. oxysporum f.sp. lycopersici with 76.94% growth inhibition. Conclusion: Fusarium oxysporum is prevalent in tomato growing farmlands covered in this study. T. viride identified in this study is an effective biocontrol agent for the identified F. oxysporum fsp. lycopersici in vitro.

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Screening of Fungal Endophytes for their Biocontrol Potential against Rhizopus sp. Isolated from Diseased Cassava (Manihot esculenta Crantz)

African Journal of Biology and Medical Research ◽

10.52589/ajbmr/dvesyirz ◽

2021 ◽

Vol 4 (2) ◽

pp. 25-37

Author(s):

Onyemaechi H.O. ◽

Obehi V.O. ◽

Felix O.

Keyword(s):

Culture Filtrate ◽

Fungal Endophytes ◽

Dual Culture ◽

Yeast Isolate ◽

Manihot Esculenta Crantz ◽

Mycelia Growth ◽

Dual Culture Assay ◽

Fusarium Sp ◽

Biocontrol Potential ◽

Rhizopus Sp

The aim of this study was to screen for the bio-control potential of fungal endophytes isolated from cassava against a test pathogen of cassava. Fungal endophytes and pathogen were isolated and identified from healthy and diseased cassava respectively. The isolated fungal endophytes were screened for their biocontrol potential against a test pathogen using the dual culture and culture filtrate assay. Fusarium sp., Botryosphaeria sp., Colletotrichum sp., yeast isolate 1 and 2 were the isolated fungal endophytes while the pathogen was Rhizopus sp. The effect of endophytes on the mycelia growth of Rhizopus sp. using the dual culture assay indicated that yeast isolate 1 & 2 and Colletotrichum sp. were effective in inhibiting the mycelia growth of the test pathogen while Fusarium sp. and Botryosphaeria sp. were not effective. The co-culture of yeast isolate 2 with the test pathogen gave the lowest mycelia growth (1.66a±0.09) at day 2. The effect of endophytic culture filtrate on the mycelia growth of Rhizopus sp. showed that Fusarium sp. gave the lowest mycelia growth in the three days observed. The findings from this study suggested that the test endophytes have biocontrol potential against Rhizopus sp. The biocontrol abilities of the test endophytes vary using the dual culture and culture filtrate assay.

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In vitro and in vivo Assay for Assessment of the Biological Control Potential of Streptomyces sp. KT

Journal of Plant Studies ◽

10.5539/jps.v7n1p10 ◽

2017 ◽

Vol 7 (1) ◽

pp. 10

Author(s):

Tatsuya Ohike ◽

Minori Maeda ◽

Tetsuya Matsukawa ◽

Masahiro Okanami ◽

Shin’ichiro Kajiyama ◽

...

Keyword(s):

Biological Control ◽

Suppressive Effect ◽

Phytopathogenic Fungi ◽

Dual Culture ◽

Biological Control Agents ◽

Bipolaris Oryzae ◽

Biological Control Potential ◽

Dual Culture Assay

Rhizoctonia solani is fungal plant pathogen that infects many different host plants. Recently, biological control agents that are friendly to the environment and ecosystems have attracted much attention as an alternative to the use of chemical fungicide which have been used worldwide to control soil borne pathogens including R. solani. In this study, 53 strains of actinomycetes isolated from environmental soils, and antifungal activities of them were assessed by the dual culture assay. Strain KT showed strong inhibitory activities against 8 phytopathogenic fungi. A great suppressive effect on R. solani growth was observed in the inoculation test of plants using cucumber and chin-geng-sai. In addition, infection of Bipolaris oryzae also could be suppressed in the detached leaf assay using oats. As a result of genetic analysis, it was shown that KT was a species closely related to Streptomyces lavenduligriseus NRRL B-3173T. However, as far as we know, there is no report for biological control agents using S. lavenduligriseus. This study suggests that the strain KT may useful as biological control agents to suppress various crop diseases.

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Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria

International Journal of Microbiology ◽

10.1155/2010/430873 ◽

2010 ◽

Vol 2010 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Helena Lind ◽

Anders Broberg ◽

Karin Jacobsson ◽

Hans Jonsson ◽

Johan Schnürer

Keyword(s):

Propionic Acid ◽

Starter Cultures ◽

Dual Culture ◽

Penicillium Roqueforti ◽

Glycerol Conversion ◽

Antifungal Effect ◽

Dual Culture Assay ◽

Penicillium Commune ◽

Dairy Propionibacteria ◽

Type Strains

Dairy propionibacteria are widely used in starter cultures for Swiss type cheese. These bacteria can ferment glucose, lactic acid, and glycerol into propionic acid, acetic acid, and carbon dioxide. This research examined the antifungal effect of dairy propionibacteria when glycerol was used as carbon source for bacterial growth. Five type strains of propionibacteria were tested against the yeastRhodotorula mucilaginosaand the moldsPenicillium communeandPenicillium roqueforti. The conversion of13C glycerol byPropionibacterium jenseniiwas followed with nuclear magnetic resonance. In a dual culture assay, the degree of inhibition of the molds was strongly enhanced by an increase in glycerol concentrations, while the yeast was less affected. In broth cultures, decreased pH in glycerol medium was probably responsible for the complete inhibition of the indicator fungi. NMR spectra of the glycerol conversion confirmed that propionic acid was the dominant metabolite. Based on the results obtained, the increased antifungal effect seen by glycerol addition to cultures of propionibacteria is due to the production of propionic acid and pH reduction of the medium.

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Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

Diyala Agricultural Sciences Journal ◽

10.52951/dasj.21130107 ◽

2021 ◽

Vol 13 (1) ◽

pp. 69-80

Author(s):

Majida Hadi Mahdi Alsaady ◽

Hussein Ali Salim ◽

Rakib A. Al-ani ◽

Hadi M. Aboud ◽

Jamal Talib M Al Roubaie

Keyword(s):

Fusarium Graminearum ◽

Culture Method ◽

Dry Weight ◽

Antagonistic Effect ◽

Crown Rot ◽

Control Treatment ◽

Enzyme Analysis ◽

Dual Culture ◽

Rot Disease

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere of wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. graminearum was molecularly diagnosed using the Polymerase chain reaction (PCR) technique. Each bacteria were tested for the production of catalase enzyme, oxidase enzyme, analysis of starch, analyze of gelatin, and the motility, where Azotobacter, Azospirillum, and Bacillus subtilis were positive for all tested. Fungal inhibition tests were performed by using the dual culture method and agar well diffusion technique. Among them, Streptomyces and Azospirillum exhibited potent inhibition to the growth of F. graminearum (72.14% and 66.42%) respectively, followed by B.pumillus, P.fluorescens, B. subtilis and Azotobacter ( 58.28%, 43.23%, 39.71% and 35.71%) respectively as compared with the control treatment (0.0%).The dry weight of the fungus biomass was decreased with bacteria P. fluorescens, Streptomyces sp, Azotobacter sp, Azospirillum sp, B. subtilis, and B. pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, and 0.107 g) respectively compared to the control treatment that was 0. 665 g.

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Impact of selected antagonistic fungi on Fusarium species – toxigenic cereal pathogens

Acta Mycologica ◽

10.5586/am.2008.004 ◽

2013 ◽

Vol 43 (1) ◽

pp. 29-40 ◽

Cited By ~ 19

Author(s):

Delfina Popiel ◽

Hanna Kwaśny ◽

Jerzy Chełkowski ◽

Łukasz Stępień ◽

Magdalena Laskowska

Keyword(s):

Fusarium Species ◽

Fungal Species ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Fusarium Avenaceum ◽

Dual Culture ◽

Ear Blight ◽

Yield And Quality ◽

Significant Yield ◽

The Impact

Fusarium-ear blight is a destructive disease in various cereal-growing regions and leads to significant yield and quality losses for farmers and to contamination of cereal grains with mycotoxins, mainly deoxynivalenol and derivatives, zearalenone and moniliformin. Fusarium pathogens grow well and produce significant inoculum on crop resiudues. Reduction of mycotoxins production and pathogen sporulation may be influenced by saprophytic fungi, exhibiting antagonistic effect. Dual culture bioassays were used to examine the impact of 92 isolates (belonging to 29 fungal species) against three toxigenic species, i.e. Fusarium avenaceum (Corda) Saccardo, F. culmorum (W.G.Smith) Saccardo and F. graminearum Schwabe. Both F.culmorum and F. graminearum isolates produce trichothecene mycotoxins and mycohormone zearalenone and are considered to be the most important cereal pathogens worldwide. Infection with those pathogens leads to accumulation of mycotoxins: deoxynivalenol (DON) and zearalenone (ZEA) in grains. Fusarium avenaceum isolates are producers of moniliformin (MON) and enniatins. Isolates of Trichoderma sp. were found to be the most effective ones to control the growth of examined Fusarium species. The response of Fusarium isolates to antagonistic activity of Trichoderma isolates varied and also the isolates of Trichoderma differed in their antagonistic activity against Fusarium isolates. The production of MON by two isolates of F. avenaceum in dual culture on rice was reduced by 95% to 100% by T. atroviride isolate AN 35. The same antagonist reduced the amount of moniliformin from 100 μg/g to 6.5 μg/g when inoculated to rice culture contaminated with MON, which suggests the possible decomposition of this mycotoxin.

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Exploration of multitrait antagonistic microbes against Fusarium oxysporum f.sp. lycopersici

Journal of Applied and Natural Science ◽

10.31018/jans.v11i2.2111 ◽

2019 ◽

Vol 11 (2) ◽

pp. 503-510 ◽

Cited By ~ 2

Author(s):

Prachi Singh ◽

Jyoti Singh ◽

Rahul Singh Rajput ◽

Anukool Vaishnav ◽

Shatrupa Ray ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Cost Effective ◽

Field Studies ◽

Dual Culture ◽

Cost Effective Approach ◽

Maximum Inhibition ◽

Plant Growth Promoting Activities ◽

Plant Growth Promoting ◽

Dual Culture Assay

Fusarium wilt is one of the major diseases of tomato causing extensive loss of production. Exploration of agriculturally important microbes (AIMs) for management of the tomato wilt is an ecofriendly and cost effective approach. In the present study, a total 30 Trichoderma and 30 bacterial isolates were screened in the laboratory for their biocontrol activity against Fusarium oxysporum f.sp. lycopersici (FOL). Out of all the isolates tested, Trichoderma asperellum BHU P-1 and Ochrobactrum sp. BHU PB-1 were found to show maximum inhibition of FOL in dual culture assay. Both the microbes also exhibited plant growth promoting activities such as phosphate solubilisation, production of siderophore, hydrogen cyanide (HCN), indole acetic acid (IAA) and protease activity. These microbes could be evaluated further in greenhouse and field studies for their potential use in management of Fusarium wilt of tomato.

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Evaluation of Pseudomonas fluorescens Strains, Fungicides and Non-conventional Chemicals Against Botyrotinia ricini Causing Grey Mold Disease in Castor

International Journal of Bio-resource and Stress Management ◽

10.23910/1.2021.2293 ◽

2021 ◽

Vol 12 (5) ◽

pp. 339-347

Author(s):

S. Ameer Basha ◽

◽

V. Ramya ◽

A. Sajeli Begum ◽

G. Raghavendra ◽

...

Keyword(s):

Pseudomonas Fluorescens ◽

Rhizosphere Soil ◽

Antagonistic Effect ◽

Culture Technique ◽

Soil Samples ◽

Dual Culture ◽

Integrated Disease Management ◽

Grey Mold ◽

Time Periods

A study was made to evaluate the efficacy of Pseudomonas fluorescens strains, fungicides and non-conventional chemicals against Botryotinia ricini, causing grey mold disease in castor, under in vitro conditions. Among the 40 strains isolated from rhizosphere soil samples of different crops across the State of Telangana, India, only eight strains inhibited the growth of B. ricini under dual culture technique, of which strains Pf 21 (90.56%), Pf 23 (88.89%), Pf 34 (86.11%) and Pf 36 (84.17%) were the most effective. Among the seven chemicals (four fungicides and three non-conventional chemicals) tested for their efficacy, carbendazim followed by propiconazole had significant antagonistic effect against B. ricini. Exposure of healthy castor capsules to B. ricini and P. fluorescens for different time periods revealed that strains Pf 34 and Pf 36 were effective in completely inhibiting the growth of B. ricini and hence these two strains have been identified as effective biocontrol agents, on par with carbendazim, which offer scope for sustainable and integrated disease management of grey mold disease in castor.

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Biological control of Aspergillus niger, the cause of Black-rot disease of Allium cepa L. (onion), by Penicillium species

Journal of Agrobiology ◽

10.2478/v10146-012-0003-5 ◽

2012 ◽

Vol 29 (1) ◽

pp. 23-28 ◽

Cited By ~ 9

Author(s):

Ibatsam Khokhar ◽

Muhammad Saleem Haider ◽

Irum Mukhtar ◽

Sobia Mushtaq

Keyword(s):

Biological Control ◽

Aspergillus Niger ◽

Agar Plate ◽

Antagonistic Effect ◽

Black Rot ◽

Dual Culture ◽

Penicillium Species ◽

Allium Cepa L ◽

Rot Disease ◽

Very High

Abstract The purpose of the study was to explore the control of the onion black rot pathogen, Aspergillus niger, with Penicillium species as biological control agents. Fourteen Penicillium species were isolated from the rhizosphere of different plants. In dual culture agar plate assays, these isolates showed very high antagonistic effects on the growth of A. niger mycelium. Penicillium roqueforti and P. viridicatum greatly inhibited the growth of A. niger by 66% and 60%, respectively, followed by P. bilaii (57%) and P. olsonii (53%). However, it was also observed that the Penicillium species completely overgrew the A. niger colony. The study revealed that some species of the genus Penicillium possessed a high antagonistic effect on the onion black rot pathogen.

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