scholarly journals Changes in Acute Phase Proteins in Bitches after Laparoscopic, Midline, and Flank Ovariectomy Using the Same Method for Hemostasis

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2223
Author(s):  
Ayla Del Romero ◽  
Belén Cuervo ◽  
Pau Peláez ◽  
Laura Miguel ◽  
Marta Torres ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Serum Levels ◽  
Paraoxonase 1 ◽  
Surgical Trauma ◽  
C Reactive Protein ◽  
Post Intervention ◽  
Reactive Protein ◽  
Post Surgery ◽  
Serum Crp

Acute phase proteins (APP) are biomarkers of systemic inflammation, which allow monitoring the evolution of diseases, the response to treatments, and post-operative complications. Ovariectomy (OVE) is frequently performed in veterinary medicine and can be a useful model to evaluate surgical trauma and inflammation in the bitch. The objective was to investigate and compare the acute phase response (APR) after applying three different OVE techniques by measuring serum levels of C-reactive protein (CRP), haptoglobin (Hp), albumin (Alb), and paraoxonase-1 (PON-1). Forty-five intact bitches were included in the study, being randomly distributed into three groups: laparoscopic OVE (L), midline OVE (M), and flank OVE (F). Serum CRP, Hp, Alb, and PON-1 were measured before surgery, 1, 24, 72, and 168 h post-intervention. CRP levels increased significantly 24 h post-surgery in the M and F groups, but no significant variation was observed in the L group at any time of the study period. Hp was significantly higher in group L than in group F 72 h post-surgery. Alb and PON-1 showed no statistical difference among groups or among sampling periods. CRP response suggests that the use of laparoscopic procedures produce lower inflammation compared to open conventional approaches when performing OVE in the bitch.

scholarly journals C-reactive protein and cytokines in the diagnosis of neonatal sepsis

2006 ◽  
Vol 59 (11-12) ◽  
pp. 545-549 ◽  
Author(s):  
Borisav Jankovic ◽  
Dobrila Veljkovic ◽  
Srdjan Pasic ◽  
Zorica Rakonjac ◽  
Dragana Jevtic ◽  
...  
Keyword(s):  
Acute Phase ◽  
Neonatal Sepsis ◽  
Current Knowledge ◽  
Systemic Infection ◽  
Serum Levels ◽  
C Reactive Protein ◽  
Correct Treatment ◽  
Acute Phase Reactants ◽  
Reactive Protein ◽  
Serum Crp

Introduction. Accurate evaluation and correct treatment of neonates for possible sepsis still represent the most challenging clinical tasks. Early diagnosis of neonatal sepsis is largely based on the measurement of serum concentrations of different mediators of systemic inflammation, as well as, on a group of proteins named acute phase reactants. Among acute phase reactants, C-reactive protein (CRP) has been the most extensively used and investigated so far. Synthesis and biological role of CRP. This article reviews current knowledge on the synthesis, structure and biologic roles of CRP. Also, we present our original results in regard to the kinetics of serum CRP concentration during the first 24 hours of systemic infection, as well as different patterns of CRP dynamics associated with the initial choice of antibiotics, complications and the final outcome of systemic infection. Interleukins and procalcitonin in diagnosis of sepsis. Because CRP is specific, but somewhat late marker of neonatal sepsis, possible diagnostic use of other indicators of inflammation, i.e. interleukins 6 and 8, and procalcitonin during neonatal sepsis is also considered. The theoretical advantage of these early indicators is discussed in comparative analysis of the time of their activation after initial infectious stimuli. Conclusion. In conclusion, we point to the diagnostic accuracy of serial measurements of serum CRP levels. As an alternative, simultaneous measurement of CRP and serum levels using a faster marker, such as procalcitonin, is recommended.

scholarly journals Serum C-reactive protein concentrations in dogs with idiopathic epilepsy

2017 ◽  
Vol 86 (2) ◽  
pp. 79-83
Author(s):  
E. Segers ◽  
V. Martlé ◽  
S. Piepers ◽  
L. Van Ham ◽  
S. F. M. Bhatti
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Idiopathic Epilepsy ◽  
Control Group ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Cluster Seizures ◽  
Serum Crp

Inflammatory reactions in dogs are associated with systemic changes in serum, called the acute phase response; changes in the concentration of acute phase proteins in the serum take place. C-reactive protein (CRP) is a positive acute phase protein, which increases during inflammation. The role of inflammation in epilepsy remains unclear. In this study, the inflammatory response in dogs with idiopathic epilepsy (IE) was investigated. The aims of the study were: 1. to measure serum CRP concentrations in dogs with IE and in healthy dogs, 2. to measure serum CRP concentrations in dogs with acute cluster seizures and in dogs with isolated seizures and 3. to observe the evolution of serum CRP concentrations in time after the last seizure. This study showed no significant differences in serum CRP concentrations between dogs with IE (7.8 mg/l) and dogs of the control group (8.3 mg/l). Furthermore, the results showed higher mean serum CRP concentrations in dogs with IE exhibiting cluster seizures (11,8 mg/l) than in dogs with isolated seizures (5.7 mg/l). However, these results were not statistically significant (P = 0,077). Finally, no statistically significant decrease in serum CRP concentrations was seen with time after the last epileptic seizure in dogs with IE (P = 0,077).

Acute phase response after myocardial infarction: Correlation between serum levels of cytokines and C-reactive protein

1990 ◽  
Vol 68 (21) ◽  
pp. 1083-1083 ◽  
Author(s):  
H. Tilg ◽  
J. Mair ◽  
M. Herold ◽  
W. E. Aulitzky ◽  
P. Lechleitner ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Levels ◽  
Phase Response ◽  
C Reactive Protein ◽  
Reactive Protein

The acute phase response and C-reactive protein

2020 ◽  
pp. 2199-2207
Author(s):  
Mark B. Pepys
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Phase Response ◽  
Serum Amyloid ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Serum Amyloid A Protein

The acute phase response—trauma, tissue necrosis, infection, inflammation, and malignant neoplasia induce a complex series of nonspecific systemic, physiological, and metabolic responses including fever, leucocytosis, catabolism of muscle proteins, greatly increased de novo synthesis and secretion of a number of ‘acute phase’ plasma proteins, and decreased synthesis of albumin, transthyretin, and high- and low-density lipoproteins. The altered plasma protein concentration profile is called the acute phase response. Acute phase proteins—these are mostly synthesized by hepatocytes, in which transcription is controlled by cytokines including interleukin 1, interleukin 6, and tumour necrosis factor. The circulating concentrations of complement proteins and clotting factors increase by up to 50 to 100%; some of the proteinase inhibitors and α‎1-acid glycoprotein can increase three- to fivefold; but C-reactive protein (CRP) and serum amyloid A protein (an apolipoprotein of high-density lipoprotein particles) are unique in that their concentrations can change by more than 1000-fold. C-reactive protein—this consists of five identical, nonglycosylated, noncovalently associated polypeptide subunits. It binds to autologous and extrinsic materials which contain phosphocholine, including bacteria and their products. Ligand-bound CRP activates the classical complement pathway and triggers the inflammatory and opsonizing activities of the complement system, thereby contributing to innate host resistance to pneumococci and probably to recognition and safe ‘scavenging’ of cellular debris. Clinical features—(1) determination of CRP in serum or plasma is the most useful marker of the acute phase response in most inflammatory and tissue damaging conditions. (2) Acute phase proteins may be harmful in some circumstances. Sustained increased production of serum amyloid A protein can lead to the deposition of AA-type, reactive systemic amyloid.

scholarly journals Acute phase proteins in bitches subjected to conventional and minimally invasive ovariohysterectomy

2018 ◽  
Vol 38 (11) ◽  
pp. 2124-2128 ◽  
Author(s):  
Elizabeth M.S. Schmidt ◽  
Camila P. Rubio ◽  
Funmilola Thomas ◽  
João C.P. Ferreira ◽  
David P. Eckersall
Keyword(s):  
Minimally Invasive ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Serum Haptoglobin ◽  
Hemoglobin Binding ◽  
Mixed Breed ◽  
Significant Difference ◽  
Haptoglobin Concentration

ABSTRACT: The aim of this study was to evaluate and to compare the possible inflammatory changes by screening acute phase proteins concentrations in healthy bitches subjected to ovariohysterectomy. Minimally invasive and conventional (laparotomy) ovariohysterectomies were performed in 17 client-owned adult female mixed breed dogs. Nine animals were subjected to minimally invasive and eight animals to conventional ovariohysterectomy. Blood samples were taken before surgery, 24, 48 hours, and seven days postoperatively. Serum C-reactive concentration was determined by a commercial ELISA kit and serum haptoglobin concentration was measured via hemoglobin binding assay, both previously validated for use in dogs. As the data did not meet the normal distribution criteria, the nonparametric Kruskall-Wallis was performed to compare quantitative variables between groups. One-way ANOVA and the Friedman test were used for multiple comparisons between time points, with a P<0.05 considered significant. C-reactive protein concentration was significantly different (P<0.0001) at 24 hours postoperatively between groups. There was no significant difference in haptoglobin concentration between groups. C-reactive protein and haptoglobin concentrations were significantly different at 24 and 48 hours postoperatively for minimally invasive and conventional ovariohisterectomies. These findings provided an overview of the short-term inflammatory effects produced by minimally invasive and conventional ovariohysterectomies.

scholarly journals Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

1986 ◽  
Vol 32 (5) ◽  
pp. 743-747 ◽  
Author(s):  
J Braun ◽  
T Schultek ◽  
K F Tegtmeier ◽  
A Florenz ◽  
C Rohde ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Immunoglobulin A ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Coefficients Of Variation ◽  
Alpha 1 Antitrypsin ◽  
Heel Prick ◽  
Secretory Immunoglobulin

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

Abstract B87: Acute-phase proteins (C-reactive protein and Serum Amyloid A) and post-diagnosis mammographic density in breast cancer survivors

2012 ◽  
Vol 5 (11 Supplement) ◽  
pp. B87-B87
Author(s):  
Anne Dee ◽  
Roberta McKean-Cowdin ◽  
Anne McTiernan ◽  
Richard N. Baumgartner ◽  
Kathy B. Baumgartner ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Survivors ◽  
Mammographic Density ◽  
Acute Phase ◽  
Breast Cancer Survivors ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A

Evolutionary changes in acute-phase proteins in alcoholic hepatocellular diseases.

1983 ◽  
Vol 29 (1) ◽  
pp. 45-47 ◽  
Author(s):  
C Perier ◽  
A Chamson ◽  
R Engler ◽  
J Frey
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Hepatic Disease ◽  
Serum Transferrin ◽  
Chronic Alcohol ◽  
C Reactive Protein ◽  
Chronic Alcohol Consumption ◽  
Reactive Protein ◽  
Evolutionary Changes ◽  
Two Stages

Abstract We studied the pattern of acute-phase proteins (orosomucoid, C-reactive protein, and haptoglobin) in hepatocellular deficiency due to chronic alcohol consumption, characterized by a decrease in serum transferrin concentration. We found that their patterns could vary independently of hepatocellular deficiency, but depend on the progression of hepatic disease. The most useful protein for discriminating the stage of inflammatory reaction is orosomucoid. In moderate hepatocellular deficiency, acute-phase proteins are increased independently of the decrease in transferrin, whereas in severe hepatocellular deficiency the acute-phase proteins are also decreased. Thus, it is possible to distinguish the two stages of hepatocellular deficiency by following changes in the concentration of orosomucoid.

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