scholarly journals In Vitro Neutral Detergent Cellulase Method and Chemical Composition to Predict In Vivo Fermentable Organic Matter of Roughages

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1594
Author(s):  
Yue Liu ◽  
Rui Li ◽  
Hao Wu ◽  
Qingxiang Meng ◽  
Muhammad Zahoor Khan ◽  
...  
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Energy Production ◽  
Crop Residues ◽  
Correlation Equations ◽  
Strong Correlations ◽  
Current Experiment

In vivo fermentable organic matter (FOM) reflects the energy production and the potential of rumen’s microbial protein synthesis. However, the in vivo method with fistulated animals for FOM measurement compromises animal welfare and is laborious as well as expensive. Although the alternative in situ nylon bag technique has been widely used, it is also costly and requires rumen liquor. Therefore, the present study was performed to compare the in situ nylon bag technique with the in vitro neutral detergent cellulase (NDC) method or chemical composition to estimate in vivo FOM of roughages. For this purpose, we selected 12 roughages, including six each from forages and crop residues. Our results have shown the strong correlation equations between FOMin situ and FOMNDCFOM NDC of forages (n =6; R2 = 0.79), crop residues (n =6; R2 = 0.80), and roughages (n = 12; R2 = 0.84), respectively. Moreover, there were also strong correlations between the chemical composition of roughages and FOMin situ(n = 12; R2 = 0.84–0.93) or FOMNDC (n = 12; R2 = 0.79–0.89). In conclusion, the in vitro NDC method and chemical composition were alternatives to in situ nylon bag technique for predicting in vivo FOM of roughages in the current experiment.

Palatability,in situandin vitronutritive value of dried sainfoin (Onobrychis viciifolia)

2010 ◽  
Vol 148 (6) ◽  
pp. 723-733 ◽  
Author(s):  
H. KHALILVANDI-BEHROOZYAR ◽  
M. DEHGHAN-BANADAKY ◽  
K. REZAYAZDI
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Nutritive Value ◽  
Nutrient Content ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Acid Detergent Fibre

SUMMARYThe current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used forin situandin vivoexperiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively.In situeffective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production,in vitroandin vivodetermined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.

scholarly journals Comparison of methods used to predict the in vivo digestibility of feeds in ruminants

1999 ◽  
Vol 50 (5) ◽  
pp. 825 ◽  
Author(s):  
S. Kitessa ◽  
G. G. Irish ◽  
P. C. Flinn
Keyword(s):  
Chemical Composition ◽  
Energy Content ◽  
Starch Content ◽  
Gas Production ◽  
Near Infrared Reflectance ◽  
Laboratory Methods ◽  
Feed Samples

Digestibility is a very useful index of the energy content of ruminant feeds, but cheaper and quicker laboratory methods are required as an alternative to the ultimate measure of in vivo digestibility using animals. These methods involve either prediction of digestibility from chemical composition or in vitro and in situ simulation of the digestion process. This review presents a range of chemical and in vitro techniques for predicting digestibility, together with an assessment of their advantages and limitations, particularly the degree to which they account for the sources of variation in in vivo digestibility in ruminants. In situ digestion of feed samples in the actual rumen environment is probably the most accurate of the non in vivo procedures, but is not suitable for routine application. Thein vitro gas production technique offers no advantages in prediction of total tract digestibility, but is useful for screening cereal grains for rate of starch hydrolysis in the rumen. The preferred procedure for routine laboratory prediction of digestibility is the pepsin-cellulase technique, provided amylase is included or high temperature digestion is used for samples high in starch content. Prediction of digestibility from chemical composition is not recommended. The optical technique of near infrared reflectance spectroscopy can be calibrated against any of the methods outlined in this review, and is unsurpassed in terms of speed and repeatability. Direct NIR prediction of in vivo digestibility is also possible, but is limited by the lack of adequate numbers of feed samples with known in vivo values. Future work should be aimed at filling this gap and also improving the accuracy of laboratory methods for predicting the digestibility of low quality feeds.

Nutritive value of the olive leaf: effects of cultivar, season of harvesting and system of drying

1992 ◽  
Vol 119 (2) ◽  
pp. 205-210 ◽  
Author(s):  
A. Cabrera-Gomez ◽  
A. Garrido ◽  
J. E. Guerrero ◽  
V. Ortiz
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Nutritive Value ◽  
Drying Procedures ◽  
Significant Difference ◽  
Different Seasons ◽  
Organic Matter Digestibility ◽  
Olive Trees

SUMMARYLeaves of olive trees (Olea europaea L.) of eight cultivars, Manzanilla, Picual, Hojiblanca, Edremit, Conservolia, Picholine-Marroqui, Chemlali and Frantoio, were collected in different seasons and years in Córdoba, Spain. Their chemical composition and in vitro organic matter digestibility values were determined. Differences in composition and nutritive value between cultivars, years and seasons were studied in Manzanilla, Picual and Hojiblanca, the three most important local cultivars. There were significant differences both between cultivars and seasons (P < 0·05) and a non-significant difference between years.The effect of various drying procedures (fresh, oven-dried, air-dried indoors or air-dried outdoors) on chemical composition, in vitro digestibility and in vivo digestibility in sheep was also examined. The in vivo organic matter digestibility of fresh leaves was significantly higher than that of dried leaves. No difference was found between leaves air-dried indoors and oven-dried leaves. However, leaves air-dried outdoors were significantly less digestible. Quick drying in an oven reduced the digestibility of crude protein and acid detergent fibres compared to air drying indoors. There was a significant decrease in digestibility of 11% when leaves were dried separated from the branches as compared to leaves dried on the branches.

scholarly journals In vitro estimation of rumen fermentable organic matter using rumen fluid and a cell free preparation of rumen fluid

1994 ◽  
Vol 42 (4) ◽  
pp. 343-356 ◽  
Author(s):  
J.W. Cone ◽  
A.H. Van Gelder ◽  
E.T. Veerman ◽  
A.M. Van Vuuren
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Good Prediction ◽  
Feed Composition ◽  
In Vitro Methods ◽  
In Situ Method ◽  
A Cell

The amount of microbial protein leaving the rumen is considered as a function of the amount of rumen-fermentable organic matter (FOM) in the rumen. FOM can be calculated using tables, or estimated by in situ incubation, but both methods have some drawbacks. In vitro methods were therefore developed to estimate FOM, using fresh rumen fluid or a cell-free preparation of rumen fluid. Results were compared with the in situ method and a method using chemical feed composition. The in vitro methods gave a good prediction of the in situ estimation of FOM for the majority of feeds. For some feeds rich in starch or fat, the correlation was poor. Because no in vivo data of FOM were available, it could not be determined whether the in vitro or in situ methods gave false results. However, arguments suggest that the in situ method is not suitable for some feeds.

Prediction of in vivo organic matter digestibility of beef cattle diets from degradation parameters estimated from in situ and in vitro incubations

2019 ◽  
Vol 157 (9-10) ◽  
pp. 711-720
Author(s):  
Pedro Del Bianco Benedeti ◽  
Sebastião de Campos Valadares Filho ◽  
Diego Zanetti ◽  
Fabyano Fonseca e Silva ◽  
Breno de Castro Silva ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Fixed Effects ◽  
Stepwise Regression ◽  
Meta Analysis ◽  
Insoluble Fraction ◽  
Water Soluble

AbstractThe objective of this meta-analysis study was to develop and validate equations estimated from in situ and in vitro methods to predict in vivo ruminal digestibility of organic matter (OM) of beef cattle diets. The database was composed of individual data of 23 diets from six experiments. Information collected from these studies was: in vivo digestibility and degradation parameters of OM calculated from in situ and in vitro incubations. The values of estimated times for the in situ and in vitro incubations to access in vivo digestibility of OM, and differences between degradation at 24, 48 and 72 h (in situ and in vitro) and in vivo digestibility were analysed in a model that included the fixed effects of forage neutral detergent fibre level. Thereafter, a multiple stepwise regression was carried out using OM digestibility as a dependent variable and degradation parameters (A = water-soluble fraction; B = potentially degradable water-insoluble fraction; and kd = degradation rate of fraction B) as independent variables. Equation validation was performed using data from a seventh experiment that have the same methods than previous studies. Stepwise regression results showed that the kd contributed significantly in most of the algorithms derived to predict in vivo digestibility. Validation analysis showed that equations developed from both in vitro and in situ incubations accurately estimated the in vivo digestibility of OM (P > 0.05). Our results suggest that equations developed to estimate OM digestibility showed both precision and accuracy; however, in situ method presented better results than in vitro.

scholarly journals A comparison of in situ and in vitro methods to estimate in vivo fermentable organic matter of forages in ruminants

2004 ◽  
Vol 52 (1) ◽  
pp. 29-45 ◽  
Author(s):  
J.M.J. Gosselink ◽  
J.P. Dulphy ◽  
C. Poncet ◽  
S. Tamminga ◽  
J.W. Cone
Keyword(s):  
Organic Matter ◽  
In Vitro Methods

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

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