scholarly journals Enhancement of Bioreactor Performance Using Acclimatised Seed Sludge in Anaerobic Treatment of Chicken Slaughterhouse Wastewater: Laboratory Achievement, Energy Recovery, and Its Commercial-Scale Potential

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3313
Author(s):  
Tuan Nurfarhana Tuan Mohd Marzuki ◽  
Syazwani Idrus ◽  
Mohammed Ali Musa ◽  
Abdul Malek Abdul Wahab ◽  
Nur Syakina Jamali ◽  
...  

Lack of good management practice of chicken slaughterhouse wastewater (CSWW) has caused pollution into water bodies. In this study, the potential of seed sludge acclimatised modified synthetic wastewater (MSWW) on bioreactor performance and energy recovery of CSWW treatment was investigated. Two sets of upflow anaerobic sludge blanket (UASB) reactors were employed. The seed sludge in UASB 2 was acclimatised with MSWW for 30 days. In UASB 1, no acclimatisation process was undertaken on seed sludge for control purposes. After the acclimatisation process of UASB 2, both reactors were supplied with CSWW under the same condition of organic loading rate (OLR = 0.5 to 6 gCOD/L/d) and mesophilic condition (37 °C). COD removal efficiencies of UASB 2 were >80% all through the steady-state of the OLR applied. Meanwhile, a drastic decrease in overall performance was observed in UASB 1 when the OLR was increased to 3, 4, 5, and 6 gCOD/L/d. Energy recovery from laboratory scale and projected value from commercial-scale bioreactor were 0.056 kWh and 790.49 kWh per day, respectively. Preliminary design of an on-site commercial-scale anaerobic reactor was proposed at a capacity of 60 m3.

2015 ◽  
Vol 35 (2) ◽  
pp. 331-339 ◽  
Author(s):  
RONALDO FIA ◽  
ERLON L. PEREIRA ◽  
FÁTIMA R. L. FIA ◽  
DÉBORA G. EMBOABA ◽  
EMANUEL M. GOMES

This study aimed to evaluate the start-up of a horizontal anaerobic fixed bed reactor (HAFBR) followed by an upflow anaerobic sludge blanket (UASB) for the slaughterhouse wastewater treatment. HAFBR was filled with bamboo rings and had 1.2 m in length, 0.10 m in diameter and volume of 7.5 L. The UASB had the volume of 15 L. The HAFBR and UASB operated at organic loading rate and hydraulic retention time average of 8.46 and 3.77 kg m-3 d-1 of COD and 0.53 and 0.98 days, respectively. During 150 days of monitoring system it was found pH 6.8, relatively high values of bicarbonate alkalinity (> 1000 mg L-1) and reduced values of volatile acids (70 to 150 mg L-1), which afforded average removal efficiencies of COD total and total suspended solids of the order of 31 and 23% in HAFBR and 79% and 63% in UASB. It can be concluded that the generation and consumption of bicarbonate alkalinity and total volatile acids, thereby maintaining the pH during the study indicated stable operation of the reactors. The COD removal in the reactors was satisfactory especially when it considers that the assessment was conducted in a period of adaptation of organisms to the effluent and also the high organic load applied during this period.


2014 ◽  
Vol 953-954 ◽  
pp. 1105-1108 ◽  
Author(s):  
Seni Karnchanawong ◽  
Kraiwet Kabtum

The objective of this study was to investigate the toxicity of Na+and K+ions on performance of upflow anaerobic sludge blanket (UASB) system. Three laboratory-scale UASB reactors, 15.8 - l working volume, were employed with 1 reactor operated as control. They were loaded at organic loading rate (OLR) of 5 kg COD/(m3-d), treating synthetic wastewater with COD concentration ~ 5000 mg/l. Na+and K+ions were added in the range of 1010 - 7180 and 41 - 7320 mg/l, respectively. No toxicity was observed at influent Na+and K+concentrations up to 3340 and 2750 mg/l, respectively. Slight inhibitions on COD removal were founded at Na+and K+concentrations of 4610 and 3920 mg/l, respectively, but moderate effect on biogas production had occurred. When Na+and K+concentrations were increased to 7180 and 7320 mg/l, respectively, strong inhibitions were observed with COD removal dropped to 45.5 and 48.8 %, respectively. Ratios of biogas productions, as compared to the control reactor, were dropped to 0.31 and 0.32, respectively. Increasing cation concentrations had more detrimental effect on biogas production than COD removal.


2020 ◽  
Vol 9 (1) ◽  
pp. 32-51
Author(s):  
Revanuru Subramanyam

This research article describes start-up performance of an UASB (Upflow Anaerobic Sludge Blanket) reactor in terms of chemical oxygen demand (COD) removal efficiency, biogas production, sludge loading rate (SLR), volatile fatty acids (VFA), pH, alkalinity, total solids (TS) and volatile suspended solids (VSS), fed with synthetic wastewater with increased concentrations of glucose. The reactor was loaded up to an OLR (Organic Loading Rate) of 15 kg COD m-3 d-1 and achieved a COD removal efficiency of 82 ±3%. The results showed that digested seed sludge was successfully acclimatized and transformed finally into granular sludge within a period of 120 days. An increase in the accumulation of VFA at high OLRs showed that methanogenesis could be the rate-limiting step in the reactor operation. The SLR and VSS/TS ratio were increased with an increase in OLR. During the initial stages, uniform distribution of VSS concentration and later on maximum VSS concentration were found at port number two at a height of 350 mm. The carbon balance depicts that the maximum percentage of influent COD converted to methane COD. An increase in specific methanogenic activity values with the age of sludge confirmed the transformation of the seed sludge in to a granular sludge.


2001 ◽  
Vol 44 (4) ◽  
pp. 83-88 ◽  
Author(s):  
V. Del Nery ◽  
M. H.Z. Damianovic ◽  
F. G. Barros

This work studied the performance of the dissolved air flotation (DAF) system and the start-up and the operation of two 450 m3 UASB reactors in a poultry slaughterhouse in Sorocaba, Brazil. The DAF presented reduction efficiency of grease and fats, suspended solids and COD 50% higher. The reactors were seeded with non-adapted sludge. The average COD of the reactor influent was 2,695mg/L; and the initial organic loading rate (OLR) and the initial sludge loading rate at the start-up were 0.51 kg COD/m3.day and 0.04 kg COD/kg VTS.day, respectively. The start-up period was 144 days. During this time the reactor flow rate and OLR were gradually increased. At the reactor start-up, the maximum OLR value was 2.1 kg COD/m3.day, the COD reduction was higher than 80%, and the concentration of volatile fatsty acids (VFA) was below 100mg/L. The COD reductions, considering the reactor effluent raw COD and soluble COD were similar throughout the period studied in both reactors. The reactor effluent raw COD was approximately 10% higher than the soluble COD until the 225th day of operation. From the 225th day of operation this value increased 20%-30% due to the sludge washout. The effluent soluble COD reduction, the effluent VFA concentration and the operational stability attested the good performance of UASB reactors in poultry slaughterhouse wastewater treatment.


Author(s):  
Mohammed Musa ◽  
Syazwani Idrus ◽  
Che Hasfalina ◽  
Nik Daud

In this study, the performance of a laboratory scale upflow anaerobic sludge blanket (UASB) reactor operating at mesophilic temperature (35 °C) was examined. Cattle slaughterhouse wastewater (CSWW) was used as the main substrate. The total and effective volumes of the reactor were 8 L and 6 L, respectively. Twelve different organic loading rates (OLR) were applied and the performance was evaluated. The chemical oxygen demand (COD) removal efficiency was more than 90% during batch study. In the continuous study, COD removal was also approximately 90% at OLR 0.4 g/L d−1 which subsequently dropped to below 50% when the loading rate increased to 15 g/L d−1. Approximately 5 L/d of biogas was obtained with high methane concentration at stages VI and XI corresponding to OLR of 2 and 10 g/L d−1, respectively. It was observed that the concentration of volatile fatty acids was low and that the alkalinity of the wastewater was sufficient to avoid acidification. Specific methane yields of 0.36 and 0.38 LCH4/g COD added were achieved at OLR 7 and 10 g/L d−1. A hydraulic retention time (HRT) of 1 day was sufficient to remove greater than 70% of COD which correspond to 89% methane concentration. Parameters like soluble COD, NH3-N, pH, alkalinity, total suspended solid (TSS), fats, oil, and grease were also investigated. The results show that the UASB reactor could serve as a good alternative for anaerobic treatment of CSWW and methane production.


Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 806 ◽  
Author(s):  
Mohammed Ali Musa ◽  
Syazwani Idrus ◽  
Hasfalina Che Man ◽  
Nik Norsyahariati Nik Daud

Cattle slaughterhouse wastewater (CSWW) with an average chemical oxygen demand (COD) and biochemical oxygen demand of 32,000 mg/L and 17,000 mg/L, respectively, can cause a severe environmental hazard if discharged untreated. Conventional upflow anaerobic sludge blanket (UASB) reactor is used in the treatment of slaughterhouse wastewater to meet the discharge standard limit of wastewater discharge set by the Department of Environment Malaysia (DOE). However, at higher loading rates the conventional systems are characterized by slow-growing microorganism resulting in long startup period, surface scum formation, and sludge washout. In this work, the performance of two laboratory scale (12 L) conventional (R1) and modified (R2) UASB reactors treating CSWW at mesophilic (36 ± 1 °C) condition were investigated. Both reactors were subjected to increasing organic loading rate (OLR) from 1.75 to 32 g L−1 day−1. The average COD, BOD5, and TSS removal efficiencies were ˃90%, at an OLR between 1.75 to 5 g L−1 day−1. The study revealed that R1 drastically reduced to 50, 53, and 43% with increasing OLR until 16 g L−1 day−1, whereas R2 maintained 76, 77, and 88% respectively, under the same OLR. Sign of reactor instability was very much pronounced in R1, showing poorly active Methanosaeta spp., whereas R2 showed a predominantly active Methanosarcina spp.


Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 490
Author(s):  
Mohammed Ali Musa ◽  
Syazwani Idrus

Anaerobic digestion technology provides an alternative route for sustainable management of organic waste. In this study, the performance of the hybrid upflow anaerobic sludge blanket (HUASB) reactor consisting of synthetic grass media as attached growth surface was investigated for the treatment of cattle slaughterhouse wastewater under mesophilic (35 ± 1 °C) condition. After acclimatization with synthetic wastewater, the reactor was loaded up to OLR 10 g L−1d−1, corresponding to 20 g COD/L at a varying hydraulic retention time (HRT) of 24, 30, 36, 42, and 48 h. The system attained a maximum COD removal efficiency of 97% total suspended solids (TSS), volatile suspended solids (VSS), fats, oil, and grease (FOG), color removal, and turbidity were found as 97%, 284 mg/L, 79%, 78%, and 91% respectively. The biogas production after 48 h was found as 38 L/d, with about 85% methane and specific methane production of 0.24 LCH4/gCODadded. The ratio of alkalinity was 0.22, while ammonia nitrogen concentration reached a maximum of 839 mg/L at a steady state. Scanning electron microscopic (SEM) analysis revealed a predominance of Methanosarcina bacteria with the coccoidal shape at the end of the performance study. Therefore, the results of the experiment showed that increasing HRT significantly affects the performance of the system.


Author(s):  
Mohammed Ali Musa ◽  
Syazwani Idrus ◽  
Mohd Razif Harun ◽  
Tuan Farhana Tuan Mohd Marzuki ◽  
Abdul Malek Abdul Wahab

Cattle slaughterhouses generate wastewater that is rich in organic contaminant and nutrients, which is considered as high strength wastewater with a high potential for energy recovery. Work was undertaken to evaluate the efficiency of the 12 L laboratory scale conventional and a modified upflow anaerobic sludge blanket (UASB) reactors (conventional, R1 and modified, R2), for treatment of cattle slaughterhouse wastewater (CSWW) under mesophilic condition (35 ± 1 °C). Both reactors were acclimated with synthetic wastewater for 30 days, then continuous study with real CSWW proceeds. The reactors were subjected to the same loading condition of OLR, starting from 1.75, 3, 5 10, 14, and 16 g L−1d−1, corresponding to 3.5, 6, 10, 20, 28, and 32 g COD/L at constant hydraulic retention time (HRT) of 24 h. The performance of the R1 reactor drastically dropped at OLR 10 g L−1d−1, and this significantly affected the subsequent stages. The steady-state performance of the R2 reactor under the same loading condition as the R1 reactor revealed a high COD removal efficiency of 94% and biogas and methane productions were 27 L/d and 89%. The SMP was 0.21 LCH4/gCOD added, whereas the NH3-N alkalinity ratio stood at 651 mg/L and 0.2. SEM showed that the R2 reactor was dominated by Methanosarcina bacterial species, while the R1 reactor revealed a disturb sludge with insufficient microbial biomass.


2015 ◽  
Vol 72 (12) ◽  
pp. 2236-2242 ◽  
Author(s):  
L. I. Borges ◽  
C. M. López-Vazquez ◽  
H. García ◽  
J. B. van Lier

In this study, nitrite reduction and methanogenesis in a single-stage upflow anaerobic sludge blanket (UASB) reactor was investigated, using high-strength synthetic domestic wastewater as substrate. To assess long-term effects and evaluate the mechanisms that allow successful nitrite reduction and methanogenesis in a single-stage UASB, sludge was exposed to relatively high nitrite loading rates (315 ± 13 mgNO2−-N/(l.d)), using a chemical oxygen demand (COD) to nitrogen ratio of 18 gCOD/gNO2−-N, and an organic loading rate of 5.4 ± 0.2 gCOD/(l.d). In parallel, the effects of sludge morphology on methanogenesis inhibition were studied by performing short-term batch activity tests at different COD/NO2−-N ratios with anaerobic sludge samples. In long-term tests, denitrification was practically complete and COD removal efficiency did not change significantly after nitrite addition. Furthermore, methane production only decreased by 13%, agreeing with the reducing equivalents requirement for complete NO2− reduction to N2. Apparently, the spatial separation of denitrification and methanogenesis zones inside the UASB reactor allowed nitrite reduction and methanogenesis to occur at the same moment. Batch tests showed that granules seem to protect methanogens from nitrite inhibition, probably due to transport limitations. Combined COD and N removal via nitrite in a single-stage UASB reactor could be a feasible technology to treat high-strength domestic wastewater.


2012 ◽  
Vol 2 (2) ◽  
pp. 59-67 ◽  
Author(s):  
P. C. Vieira ◽  
M. von Sperling

We aimed to evaluate the performance and cost savings of an innovative design of a trickling filter (TF) for small population sizes, developed at the Federal University of Minas Gerais, Brazil referred to as an open trickling filter (OTF). The OTF had no side walls and no perforated bottom slab, and was applied for the post-treatment of sanitary sewage from an upflow anaerobic sludge blanket (UASB) reactor. The OTF had crushed-stone packing (3.5 m high) and was operated with an average surface hydraulic loading rate of 4.1 m3 m−2 d−1 and an average volumetric organic loading rate of 0.10 kg BOD m−3 d−1 (biochemical oxygen demand). The average concentrations obtained at the OTF effluent were 48 mg TSS L−1 (total suspended solids), 132 mg COD L−1 (chemical oxygen demand), 51 mg BOD L−1, 19 mg TKN L−1 (total Kjeldahl nitrogen), 16 mg NH4+-N L−1 and 10 mg NO3−-N L−1, complying with local discharge standards. Analysis of the construction costs indicated savings of 74% compared to conventional TF. Based on the performance, compactness, simplicity and reduced capital costs, it is believed that the proposed OTF is a good alternative for small communities, especially in developing countries.


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