scholarly journals The Effect of Strain and Rearing Medium on the Chemical Composition, Fatty Acid Profile and Carotenoid Content in Silkworm (Bombyx mori) Pupae

Animals ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 103 ◽  
Author(s):  
Camilla Chieco ◽  
Lucia Morrone ◽  
Giampaolo Bertazza ◽  
Silvia Cappellozza ◽  
Alessio Saviane ◽  
...  
Keyword(s):  
Artificial Diet ◽  
Waste Product ◽  
Carotenoid Content ◽  
Natural Food ◽  
Mulberry Leaves ◽  
Silkworm Pupae ◽  
Golden Yellow ◽  
Lower Protein ◽  
Feeding Substrate ◽  
Nutritive Composition

The overexploitation of fishmeal and soy for the feedstuff industry has provided an opportunity to employ insects as an unconventional and more environmental friendly protein source. The evaluation of the nutritive properties of different insect species has consequently become a priority. The present study compares the pupal nutritive composition of two silkworm strains (White Cocoon Polyhybrid and Golden Yellow Cocoon Nistari) fed on two different rearing media (fresh mulberry leaves and a commercial artificial diet). Our results provide evidence that the composition of the feeding substrate strongly influences the fat and protein content of silkworm pupae. The two tested strains had higher fat and lower protein contents when fed with silkworm natural food (mulberry leaves) with respect to the commercial artificial diet. The analysis also showed that the n3/n6 ratio was affected almost exclusively by the feed substrate factor. On the contrary, the carotenoid content in pupae was specifically determined by the strain. The study identifies the interesting opportunity offered by silkworm pupae, which are usually a waste product of the silk-reeling process, to be used as alternative animal protein sources in a fully-closed circular production.

scholarly journals Investigation of the protein profile of silkworm (Bombyx mori) pupae reared on a well-calibrated artificial diet compared to mulberry leaf diet

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6723 ◽  
Author(s):  
Cristina Lamberti ◽  
Francesco Gai ◽  
Simona Cirrincione ◽  
Marzia Giribaldi ◽  
Micol Purrotti ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Artificial Diet ◽  
Protein Profile ◽  
Hormone Secretion ◽  
Differentially Expressed ◽  
Food Ingredient ◽  
Male And Female ◽  
Nutritional Profile ◽  
Mulberry Leaves ◽  
Silkworm Pupae

Background Silkworm pupae is the main by-product of the sericulture industry with an interesting nutritional profile, especially in terms of proteins. In consideration of its possible use as a food or food ingredient in Western countries, a comparative proteomic experiment has been performed to investigate the differences of the protein profile of male and female silkworm pupae reared on mulberry leaves or on an artificial diet. Methods The nutritional profile of lyophilized silkworm pupae in terms of dry matter and ash was evaluated according to the AOAC procedures, the total nitrogen content was determined by a nitrogen analyzer and the silkworm pupae gross energy value was measured using an adiabatic calorimetric bomb. The comparative proteomic analysis was performed on male and female silkworm pupae reared on mulberry leaves or on the artificial diet. Proteins were separated by two-dimensional electrophoresis and, after a multivariate statistical analysis, the differentially expressed proteins were identified by LC-MS/MS. Results The comparative proteomic approach highlighted 47 silkworm pupae proteins differentially expressed comparing diet and gender. PCA analysis showed that seven proteins were more effective in discriminating the sex and five were more effective in discriminating the diet type. In spite of the above-mentioned differences in the silkworm pupae protein profile, no strong alteration of the pupa physiological traits have been demonstrated, suggesting a general silkworm pupae flexibility to adapt to a well-balanced artificial diet. Differences in lipid transport and metabolism were found among the experimental groups, that might have a relevant effect on the timing and on hormone secretion. This aspect may also affect silk production, as univoltine strains are the most productive. The proteomic data provided in this work, may offer a contribution in understanding also the influence of gender and farming strategy on the allergen profile of Bombyx mori, when used as food or as a food ingredient. Female silkworm pupae reared on mulberry leaves seemed to contain lower levels of known allergens than those reared in the other experimental conditions; these findings will have to be taken into account when farming B. mori for food production purposes. However, our results need to be supported by further characterization of the allergenic potential of B. mori.

Reproductive performance and transcriptome analyses of Tetranychus urticae when feeding on bean leaves and an artificial diet

2019 ◽  
Vol 24 (7) ◽  
pp. 1272-1283
Author(s):  
Yongjuan He ◽  
Jiale Lv ◽  
Endong Wang ◽  
Xuenong Xu
Keyword(s):  
Tetranychus Urticae ◽  
Artificial Diet ◽  
Mass Rearing ◽  
Differentially Expressed ◽  
Detoxification Enzymes ◽  
Adult Longevity ◽  
Natural Food ◽  
Leaf Extracts ◽  
Transcriptome Analyses ◽  
Bean Leaves

As an important pest, Tetranychus urticae fed on thousands of host plants and showed strong capability in host adaptation. However, hardly any success artificial diet has been developed for it. In this study, we compared adult longevity and reproduction of T. urticae that fed on its natural food (bean leaves) and an artificial diet with leaf extracts added, and tried to investigate the reason why the artificial diet was inefficient through transcriptome analyses. Mean adult longevity and cumulative fecundities of T. urticae was reduced by 53.4% and 93.8%, respectively. Transcriptome analyses showed that 1731 genes were differentially expressed comparing individuals fed with the artificial diet and with their natural food, among which most (77.1%) were down regulated. No significant induced expression of xenobiotic transporters and detoxification enzymes were observed when T. urticae were fed with the artificial diet. In contrast, differentially expressed genes were mainly enriched in digestive related terms, especially in lipid metabolism related pathways, with most genes down regulated. Our results indicated the significance in further investigating lipid demand and metabolism of T. urticae to improve its mass rearing techniques.

scholarly journals Comparison of Body Composition and Fatty Acid Profiles between Wild and Cultured Rio Grande Silvery Minnows

2017 ◽  
Vol 8 (2) ◽  
pp. 487-496 ◽  
Author(s):  
Madison S. Powell ◽  
Ronald W. Hardy ◽  
Alison M. Hutson ◽  
Louie A. Toya ◽  
Douglas Tave
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Artificial Diet ◽  
Condition Factor ◽  
Rio Grande ◽  
Wild Fish ◽  
Natural Food ◽  
Fatty Acid Profiles ◽  
Food Items ◽  
Supplementary Feed

Abstract Federally endangered Rio Grande Silvery Minnows (RGSM; Hybognathus amarus) were raised in one of three culture regimes: intensively, with only a hatchery diet; semi-intensively with access to natural food and hatchery diet supplementation; and with only natural food available at the Los Lunas Silvery Minnow Refugium (Los Lunas, New Mexico), a naturalized conservation refugium designed to mimic the natural environment of the RGSM in the Rio Grande. The project compared each culture regime and assessed differences and similarities in lipid and fatty acid content between feeding an artificial diet and consumption of natural food items in this species. After 117 d, whole-body lipid levels and fatty acid profiles were measured in each group and compared with values for wild RGSM. Fish fed the hatchery diet exclusively or as supplementary feed had significantly higher percent lipid (15.5% ± 0.5% and 10.6% ± 0.1%, respectively) than fish raised without access to the diet. Both groups had significantly higher percent lipid than fish raised in the refugium or wild fish (8.3% ± 0.1% and 7.8% ± 0.2%, respectively). Condition factor differed among groups and was highest in fish fed the hatchery diet (1.00) followed by fish supplemented with the hatchery diet (0.93), refugium fish (0.91), and wild fish (0.90). In this respect, refugium fish appeared more similar to wild fish than fish fed the hatchery diet or offered the diet as a supplement. Comparison of fatty acid profiles among groups showed marked differences among wild fish, refugium fish, and those fed the hatchery diet, either exclusively or as supplementary feed. Total omega-3 fatty acids, expressed as percentage of total fatty acids, were highest in wild fish but similar among other groups. Total omega-6 fatty acids showed an opposite trend, with five to nine times higher percentages of linoleic acid observed among fish from the three culture regimes compared with wild fish. Significant differences in lipid content and fatty acid composition between wild RGSM and cultured silvery minnows reflected their respective diets and culture regimes. Given similarities in fat content and condition factor with wild RGSM, we conclude that fish in the refugium do not require supplemental feeding with an artificial diet for this type of naturalized conservation management. Results from this study show that RGSM readily forage on natural food items present and also artificial feed when available, indicating dietary plasticity, which is advantageous for fish culture and future recovery.

scholarly journals Substitution time of natural food by artificial diet on survival rate and growth of pacific white shrimp (Litopenaeus vannamei) postlarvae during rearing in low salinity media

2011 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Ferdinand Hukama Taqwa ◽  
Daniel Djokosetiyanto ◽  
Ridwan Affandi
Keyword(s):  
Survival Rate ◽  
Litopenaeus Vannamei ◽  
Crude Protein ◽  
Artificial Diet ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Natural Food ◽  
Daily Growth ◽  
Growth And Survival ◽  
Low Salinity

<p>This research was conducted to determine natural food substitution time by artificial diet   after salinity acclimatization from 20 ppt until 2 ppt, which can increase survival and growth of (<em>Litopenaeus vannamei</em>) postlarvae during rearing period. Design experiment was completely randomized design with five treatments and three replications of natural food <em>Chironomus</em> sp.  (60% of crude protein) substitution time by artificial diet (40% of crude protein) at day: 1 (A), 7 (B), 14 (C), 21 (D) and full natural food without artificial diet (E) during 28 days rearing period. White shrimp postlarvae and rearing media in this experiment based from best result of earlier research that is PL<sub>25 </sub>from acclimatization in media 2 ppt with addition of potassium 25 ppm to freshwater media.  The densities of PL<sub>25</sub> white shrimp were 20 PLs/50 liters of 2 ppt media. The result of this experiment showed that the use of artificial diet as soon as after salinity acclimatization (PL<sub>25</sub>) gave best performance production compared to which only that was given natural food <em>Chironomus</em> sp. during experiment or with treatment by artificial diet substitution at day-7, day-14 or day-21, shown with the highest value of food consumption level, protein retention, energy retention, daily growth rate and food efficiency. Survival rate of PL<sub>54</sub> was above 80% and not significant different between treatment. That is supported by chemical-physical value of water quality still in range appropriate to survival rate of white shrimp post larvae during a rearing period. The result of this experiment indicated that requirement nutrient of PL<sub>25</sub> in low salinity did not fulfilled if only rely on natural food, so that require artificial diet with nutrition content to support growth and survival rate of white shrimp post larvae more maximal.</p> <p>Key words: salinity, natural food, artificial diet, Pacific white shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tujuan penelitian ini adalah untuk menentukan waktu penggantian pakan alami oleh pakan buatan yang tepat selama masa pemeliharaan postlarva udang vaname di media bersalinitas rendah setelah melalui masa aklimatisasi penurunan salinitas dari 20 ppt hingga 2 ppt, sehingga dapat meningkatkan pertumbuhan dan kelangsungan hidup. Rancangan percobaan berupa rancangan acak lengkap dengan perlakuan yang diterapkan berupa waktu penggantian pakan alami <em>Chironomus </em>sp. (kadar protein 62%) oleh pakan buatan (kadar protein 40%) pada hari ke-1 (A), ke-7 (B), ke-14 (C), ke-21 (D) dan pakan alami (E) selama masa pemeliharaan. Postlarva udang vaname dan media pemeliharaan yang dipergunakan selama percobaan mengacu pada hasil terbaik yang didapatkan dari penelitian pendahuluan yaitu berupa PL<sub>25 </sub>hasil aklimatisasi di media bersalinitas 2 ppt  dengan penambahan kalium 25 ppm ke media air tawar pengencer. Padat tebar sebanyak 20 ekor/50 liter/wadah. Hasil percobaan menunjukkan bahwa pemberian pakan buatan yang diberikan segera setelah masa aklimatisasi salinitas (pada awal pemeliharaan PL<sub>25</sub>) memberikan performa produksi budidaya terbaik bila dibandingkan dengan yang hanya diberi pakan alami selama masa pemeliharaan maupun waktu penggantian pakan alami oleh pakan buatan pada hari ke-7, ke-14 dan hari ke-21 yang ditunjukkan dengan tingkat konsumsi pakan, retensi protein, retensi energi, laju pertumbuhan harian dan efisiensi pakan yang tertinggi.  Kelangsungan hidup di akhir pemeliharaan (PL<sub>54</sub>)  di atas 80% dan tidak berbeda nyata antar perlakuan. Hal ini ditunjang oleh nilai fisika kimia air yang berada dalam kisaran yang layak selama masa pemeliharaan. Hasil percobaan ini menunjukkan bahwa kebutuhan nutrisi pada stadia PL<sub>25</sub> di media bersalinitas rendah tidak terpenuhi jika hanya mengandalkan pakan alami sehingga perlu ditunjang dari pakan buatan dengankandungan nutrisi yang dapat mendukung pertumbuhan dan kelangsungan hidup yang lebih maksimal.</p> <p>Kata kunci: salinitas, pakan alami, pakan buatan, udang vaname.</p>

scholarly journals Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1482
Author(s):  
Hirokazu Yagi ◽  
Saeko Yanaka ◽  
Rina Yogo ◽  
Akari Ikeda ◽  
Masayoshi Onitsuka ◽  
...  
Keyword(s):  
Artificial Diet ◽  
Isotope Labeling ◽  
Whole Body ◽  
Enrichment Ratio ◽  
Silkworm Larvae ◽  
Nmr Studies ◽  
Recombinant Glycoproteins ◽  
Silkworm Pupae ◽  
Selective Isotope Labeling ◽  
Bmnpv Bacmid

Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method for isotope labeling of glycoproteins for nuclear magnetic resonance (NMR) studies using silkworm larvae reared on an artificial diet containing 15N-labeled yeast crude protein extract. Here, we further develop this method by introducing a technique for the expression of isotope-labeled glycoproteins by silkworm pupae, which has several potential advantages relative to larvae-based techniques in terms of production yield, ease of handling, and storage. Here, we fed fifth instar larvae an artificial diet with an optimized composition containing [methyl-13C]methionine, leading to pupation. Nine-day-old pupae were then injected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid for expression of recombinant human immunoglobulin G (IgG). From the whole-body homogenates of pupae, 0.35 mg/pupa of IgG was harvested, which is a yield that is five times higher than can be obtained from larvae. Recombinant IgG, thus prepared, exhibited mainly three kinds of pauci-mannose-type oligosaccharides and had a 13C-enrichment ratio of approximately 80%. This enabled selective observation of NMR signals originating from the methionyl methyl group of IgG, confirming its conformational integrity. These data demonstrate the utility of silkworm pupae as factories for producing recombinant glycoproteins with amino-acid-selective isotope labeling.

Impacts of Food Industrial Wastes on Soil and Its Utilization as Novel Approach for Value Addition

2020 ◽  
pp. 226-243
Author(s):  
Nowsheeba Rashid ◽  
Ifra Ashraf ◽  
Shazia Ramzan
Keyword(s):  
Waste Product ◽  
Food Additives ◽  
Chemical Properties ◽  
Industrial Sector ◽  
Industrial Wastes ◽  
Natural Food ◽  
Novel Approach ◽  
Physico Chemical ◽  
Chemical Properties Of Soils ◽  
Valuable Compounds

Among the various agro-industries, food processing industries are the second prime generator of wastes after domestic sewage. In the current epoch of the rapid budding world, the wastes are mounting, which robustly sway the health of ecosystems and eventually the human population. For that reason, each agro-industrial sector has critical stipulation toward the secure utilization of agro-materials all the way through recycling of wastes. A crude disposal and littering of these waste materials frequently signifies a problem that is additionally provoked by different legal restrictions. Inadequate management of these solid waste constituents could lead to drastic change in physico-chemical properties of soils. The waste product, which is discarded into the environment, is loaded with valuable compounds. They are new, innate, and monetary sources of colorants, protein, dietary fiber, flavoring, antimicrobials, and antioxidants, which can be utilized in the food industry as a basis of natural food additives.

scholarly journals Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet

Insects ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 851
Author(s):  
DaoYuan Qin ◽  
GenHong Wang ◽  
ZhaoMing Dong ◽  
QingYou Xia ◽  
Ping Zhao
Keyword(s):  
Metabolic Profiling ◽  
Artificial Diet ◽  
Protein Biosynthesis ◽  
Physical Development ◽  
Gas Chromatography Mass Spectrometry ◽  
Artificial Diets ◽  
Mulberry Leaves ◽  
Leaf Meal ◽  
Mulberry Leaf ◽  
Silkworm Bombyx Mori

Metabonomics accurately monitors the precise metabolic responses to various dietary patterns. Metabolic profiling allows simultaneous measurement of various fecal metabolites whose concentrations may be affected by food intake. In this study, we analyzed the fecal metabolomes of silkworm (Bombyx mori) larvae reared on fresh mulberry leaves and artificial diets. 57 differentially expressed metabolites were identified by gas chromatography–mass spectrometry. Of these, 39 were up-regulated and 18 were downregulated in the mulberry leaf meal group. Most of the amino acids, carbohydrates and lipids associated with physical development and silk protein biosynthesis were enriched in silkworms reared on mulberry leaves. In contrast, the urea, citric acid, D-pinitol, D-(+)-cellobiose and N-acetyl glucosamine levels were relatively higher in the silkworm feeding on the artificial diets. The findings of this study help clarify the association between diet and metabolic profiling.

scholarly journals Artificial Diet of Silkworms (Bombyx Mori) Improved With Bee Pollen - Biotechnological Approach in Global Centre of Excellence For Advanced Research in Sericulture and Promotion of Silk Production

2020 ◽  
Vol 77 (1) ◽  
pp. 51
Author(s):  
Adela Ramona MOISE ◽  
Lucia Lelia POP ◽  
Thomas Vicentiu VEZETEU ◽  
Bianca DOMUȚ AGOSTON ◽  
Claudia PAŞCA ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Gene Pool ◽  
Artificial Diet ◽  
Alternative Food ◽  
Bee Pollen ◽  
Mulberry Leaves ◽  
Silk Production ◽  
Biotechnological Approach ◽  
Centre Of Excellence ◽  
Continental Climate

Global Centre of Excellence for Advanced Research in Sericulture and Promotion of Silk Production (GCEARS-PSP) is an important organisation for Romanian sericulture; the main aim of the centre is the revival of Romanian sericulture, the maintenance of Bombyx mori gene pool and the development of research in this domain. The moderate continental climate in Romania does not permit the rearing of silkworms all over the year and consequently, the production of silk is related to mulberry leaves production. A dietary substituent for the mulberry leaves is therefore needed. We herein tried to develop an alternative food recipe by including different concentrations of bee pollen to the commercial artificial diet recipe and measure its impact on larvae length and weight and silk production; our results demonstrate a good adaptability of silkworms to the artificial diet, and the addition of bee pollen improves the studied parameters, but without significant differences.

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