scholarly journals Redox Biomarker Baseline Levels in Cattle Tissues and Their Relationships with Meat Quality

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 958
Author(s):  
Zoi Skaperda ◽  
Angeliki Argyriadou ◽  
Paraskevi Maria Nechalioti ◽  
Maria Alvanou ◽  
Sotiria Makri ◽  
...  

Cattle breeds or crossbreds with high productivity traits have been developed to meet a growing demand for food. When intensive farming practices are followed, animals face several challenges which can result in poor performance, compromised welfare and the reduced quality of their products. Our study aims to highlight the resting values of the physiological oxidative stress that three cattle breeds exhibit, and their potential relationship with meat quality. For this purpose, we determined the levels of five common redox biomarkers (glutathione (GSH), catalase (CAT), total antioxidant capacity (TAC), thiobarbituric reactive substances (TBARS) and protein carbonyls (CARBS)) in the tissues of three commonly used beef cattle breeds (Charolais (CHA), Limousin (LIM) and Simmental (SIM)) and their association with specific meat quality traits that depend on color, pH and texture. The results revealed that LIM cattle breed animals have elevated intrinsic antioxidant defense systems in comparison to CHA and SIM cattle breed animals. In addition, the meat quality parameters were associated with the redox biomarkers. We propose that the determination of specific antioxidant parameters in the blood might be used as potential biomarkers to predict meat quality. This would allow farmers to nutritionally intervene to improve the quality of their products.

Author(s):  
Liisa Voutila ◽  
Anne Maria Mullen ◽  
Paul Allen ◽  
Declan Troy ◽  
Eero Puolanne

Loose structure and PSE like zones on the lateral surface of porcine semimembranosus muscle have beenobserved by other researchers. However the role of connective tissue in this abnormally loose structure insemimembranosus muscle has not yet been clarified. We collected about 100g samples from 7 loosestructured and 7 normal structured Irish commercial porcine semimembranosus muscles in order tocompare the onset and peak of thermal transition temperature of intramuscular connective tissue betweenthe samples from the two groups. Meat quality parameters, ultimate pH, rough estimate for drip loss,lightness (L), redness (a) yellowness (b) and as well as electrical conductivity and reflectance were alsomeasured on the samples. Four of the samples characterized as normal by visual assessment showed DFDcharacteristic so the statistical analysis was carried out both including and excluding those samples. Inboth cases the onset (p<0.001; 56.94ºC vs. 59.82ºC) and peak (p<0.001; 62.59ºC vs. 64.06ºC) of thermaltransition temperature were significantly lower in loose structured meat than in normal structured meat.Also reflectance% was lower (p<0.01; 45.18% vs. 69.17%) and the colour lighter (higher L value; p<0.01;55.05 vs. 45.52) and more yellow (higher b value; p<0.001; 18.27 vs. 14.78) in loose structured meat thanin normal structured meat when the DFD like samples were excluded. These results indicate that loosestructure in porcine semimembranosus muscle could be attributed in part to connective tissue propertiespossibly in conjunction with PSE effects.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 336-336
Author(s):  
Juliana A Torrecilhas ◽  
Elias San Vito ◽  
Josiane Lage ◽  
Giovani Fiorentini ◽  
Tiago Simioni ◽  
...  

Abstract This trial aimed to evaluate the fat color and myoglobin concentration of three cattle breeds, supplemented in two nutritional plans (NP) during the growing phase and finished in the intensive system on pasture or conventional feedlot. One hundred and nineteen bulls from three genetic groups: Nellore (N), ½ Angus x ½ Nellore (A) and ½ Senepol x ½ Nellore (S), were randomly assigned in two NP on growing phase: mineral (M) or concentrate (C, energy protein 0.3% BW). Following the growing phase, two replicates within each treatment were randomly assigned to one of two finishing systems: pasture (P, n = 59) and conventional feedlot (CF, n = 60). Bulls were supplemented daily with 2% BW of concentrate (16% CP and 78% TDN) and slaughtered at a commercial abattoir. Steaks from longissimus muscle (LM) between 12th and 13th ribs each animal were collected for analysis of concentration myoglobin (MYO) and subcutaneous fat were removed for color analysis (parameters: L*, a*, b*, C* and h*). The data were analyzed by ANOVA using PROC MIXED procedures in SAS 9.4, with a 3×2×2 factorial arrangement. The muscle of Nellore showed greater (P = 0.006) concentration of MYO compared to Angus, however, Senepol had values similar to Nellore and Angus (4.43, 4.03 and 4.25, Nellore, Angus, and Senepol, respectively), and the muscle of animal finished on CF had lower concentration of MYO compared to pasture (P &lt; 0.0001; 4.55 and 3.93, P and CF). The subcutaneous fat of animals finished on pasture showed high (P &lt; 0.05) of L*, a*, b* and C* compared to CF. Therefore, the meat quality parameters were more affected by finished systems, the meat of animals from pasture had the lighter fat and amount of yellow pigmentation, and also presented meat with greater level of myoglobin.


2020 ◽  
Vol 43 ◽  
pp. e50347
Author(s):  
Laís de Oliveira Lima ◽  
Vinícius Carneiro de Souza ◽  
Juliana Duarte Messana ◽  
Pablo de Souza Castagnino ◽  
Ana Rebeca Castro Lima ◽  
...  

This study evaluated the effect of phosphorus (P) supplementation on the intake, digestibility, and quality of aged meat from Nellore young bulls fed on high-grain diets finished in feedlot. Forty young bulls (30 months old) with an initial body weight (IBW) of 296 ± 25 kg were used. It was distributed in a completely randomized experimental design. The treatments were: without P supplementation (CO), commercial mineral supplement (CM), and supplementation with dicalcium phosphate (DP) with 2.4, 4.2, or 5.0 g of P per kg of dry matter (DM), respectively. Diets were composed of sugarcane bagasse (200 g kg-1) plus concentrate (800 g kg-1) on a dry matter (DM) basis. The meat quality parameters analyzed were pH, color, cooking losses, shear force, and water-holding capacity. P supplementation did not affect the intake and digestibility of nutrients There was no interaction (p > 0.05%) between diets and the aging time for the meat quality parameters. However, bulls fed with DP exhibited lower pH (5.98) compared to CO and CM (6.19 and 6.14, respectively). The longer aging time increased the cooking losses and intensity of yellow (b*). Under Brazilian conditions, feedlot Nellore cattle fed with high-grain diets do not require additional mineral supplements.


1991 ◽  
Vol 71 (2) ◽  
pp. 311-319 ◽  
Author(s):  
B. C. Vincent ◽  
S. D. M. Jones ◽  
L. E. Jeremiah ◽  
J. A. Newman ◽  
M. A. Price

Three groups of once-calved (OCH) heifers were slaughtered following the weaning of their calves at 3 (OCH3, n = 32), 5 (OCH5, n = 33) and 7 (OCH7, n = 31) months postcalving. A fourth group of heifers was fed a silage/grain diet and slaughtered at 15 mo of age (C, n = 32). Alternate sides of each carcass were electrically stimulated (ES) at 475 volts for 1 min (20 pulses m−1, 60 Hz) at 45 min post-stunning. All left sides were dissected into fat, lean and bone and meat quality parameters recorded. Rib eye steaks were evaluated for palatability by a trained panel and for consumer acceptability after an aging period of 6 d. All OCH groups produced heavier carcasses with a higher level of marbling than the C group after adjustment of the data to a constant proportion of carcass fatness, but the proportional yields of lean and bone tissue were similar for all heifer groups. OCH groups produced meat that was darker with a lower drip loss and higher 45 min and 6 d pH than the C group. Consumer acceptability of rib eye steaks was similar for all heifer groups, but the OCH7 (oldest) group had a higher amount of connective tissue when evaluated by a trained panel than other heifer groups. Taste panel evaluations showed that ES increased meat tenderness and overall palatability and reduced the amount of connective tissue when compared to meat from unstimulated carcasses. ES also increased the consumer acceptability of tenderness by 14.3%, flavor by 5.6%, juiciness by 5.2% and overall palatability by 6.7% over meat from unstimulated carcasses. It was concluded that once-calved heifers could produce heavier carcasses of similar composition and meat with similar eating quality to conventionally managed nonpregnant feedlot heifers. Electrical stimulation improved the consumer acceptability of meat primarily through an improvement in meat tenderness. Key words: Beef, carcass, electrical stimulation, meat quality, once-calved heifers


2016 ◽  
Vol 24 (4) ◽  
pp. 303 ◽  
Author(s):  
S. Mattioli ◽  
M. Martino ◽  
S. Ruggeri ◽  
V. Roscini ◽  
L. Moscati ◽  
...  

<p>The aim of this trial was to study the effect of an alternative housing system on the oxidative status and meat quality of fattening rabbits. From May to June 2014, 60 rabbits of 35 d of age were reared in Mobile Arks (MA) placed on alfalfa grass and frequently moved for 40 d. To assess the health status of animals, blood samples were collected at slaughter in MA and in conventional cages (C). Meat quality parameters were also evaluated. Concerning the <em>in vivo </em>oxidative status, ark-reared rabbits showed higher thiobarbituric reactive substances values than C ones, probably for the higher motor activity due to the larger living area. The lipid percentage of <em>Longissimus lumborum </em>muscle was lower (1.22 <em>vs. </em>1.48%) in the ark group. There were no significant differences in the muscle pH, colour, water holding capacity and cooking loss. Given the higher intake of grass, rich in vitamins, carotenes, polyphenols and polyunsaturated fatty acids, the antioxidant content of meat was higher in ark-reared rabbits (7.42 <em>vs. </em>6.82 µg/g of retinol, 719.2 <em>vs. </em>683.3 ng/g of α-tocopherol, respectively). Even the fatty acid profile of MA rabbits reflected the higher intake of essential fatty acids from grass and the n-3 long chain polyunsaturated fatty acids (arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid) were almost doubled. Our study suggested that the fattening of rabbits in ark system could be a possible alternative system to improve the meat quality of rabbits.</p><p><strong><br /></strong></p>


2014 ◽  
Vol 56 (1) ◽  
pp. 1060-1065
Author(s):  
G. Holló ◽  
B. Barna ◽  
K. Nuernberg

Abstract. The aim of this observational study (field experiment) was to evaluate carcass composition and meat quality of male suckling buffalo calves. The animals were born between June and September 2011 and were kept on natural pasture in a cow/calf herd. From the end of November 2011, the suckling calves were fed only roughage as feed supplement in a feedlot until slaughter. For a generalised characterisation, the basic statistics of 18 male calves were calculated. The animals were slaughtered at an average live weight of 196.0 kg. Growth, live weight development and most carcass traits varied substantially between individual calves. The 12th rib muscle composition measured by computer tomography indicated comparable meat contents as evaluated by manual dissection of the carcass. Meat quality parameters (pH and colour) and the nutrient composition (protein, fat, ash) of longissimus muscle samples of calves were relatively similar. The intramuscular fat content of longissimus muscle was low, but shows a high variation between 0.3–1.1 %.


2018 ◽  
Vol 26 (2) ◽  
pp. 165 ◽  
Author(s):  
Zhaoming Wang ◽  
Z. He ◽  
Hongjun Li

<p>We investigated the effect of repeated freeze-thaw cycles on the quality of rabbit meat. Twenty-five Hyla rabbits were slaughtered using standard commercial procedures. A freeze-thaw procedure—i.e., seven days frozen at –18°C followed by thawing at 4°C for 12h— was repeated 5 times, and 9 <em>Longissimus thoracis et lumborum</em> muscles were randomly selected at pre-set cycles (0, 1, 2, 3, and 5). The <em>Longissimus lumborum</em> muscles were used to determine meat quality parameters, while the <em>Longissimus thoracis</em> muscles were used for chemical analysis. During the repeated freeze-thaw process, muscle pH, redness, hardness, and water holding capacity gradually decreased, whereas meat lightness and yellowness gradually increased. The amount of total volatile basic nitrogen significantly increased (<em>P</em>&lt;0.05) and exceeded the threshold value for frozen meat after 5 repeated freeze-thaw cycles. The metmyoglobin proportion, thiobarbituric acid-reactive substances (TBARS) and protein carbonyl content in rabbit meat samples increased with a higher number of freeze-thaw cycles (<em>P</em>&lt;0.05), and the proportions of these compounds were positively correlated. During the repeated freeze-thaw process, extractable haeme iron levels significantly decreased (<em>P</em>&lt;0.05), and non-haeme iron levels markedly increased (<em>P</em>&lt;0.05). An sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis indicated that the degradation of both water- and salt-soluble proteins was more prevalent in samples subjected to higher numbers of freeze-thaw cycles. Additionally, a principal component analysis identified good correlations between physicochemical properties (TBARS, protein carbonyl levels and metmyoglobin content) and quality parameters (thawing loss, redness, lightness and hardness). Taken together, we conclude that the repeated freeze-thaw process can strongly affect rabbit meat quality as well as its physicochemical properties.</p>


2013 ◽  
Vol 29 (2) ◽  
pp. 385-397
Author(s):  
S. Aleksic ◽  
Sun Fang ◽  
Liu Di ◽  
M.M. Petrovic ◽  
V. Pantelic ◽  
...  

This paper presents the results of crossing Domestic Spotted breed with beef cattle breeds in the People's Republic of China and the Republic of Serbia. China is a big country of beef production and consumption. In 2012, beef production in China was 5,540,000 tons, which accounted for 9.7% of the global beef production, ranking the third in the world. The main sources of China?s beef are from crossbreeding cattle (native breed crossbred with foreign beef cattle). Simmental cattle are the most-widely used beef cattle in China?s improved beef cattle. China has cultivated its own Simmental after over 40 years? crossbreeding and improvement. China?s consumers mainly have three demands for beef quality as follows: expensive beef produced from Wagyu crossbreed with better marbling; lean beef from Simmental, Charolais and Limousin crossbreeds, top parts supplied to hotels while common parts to supermarkets; veal from cow calves. Chinese researchers are carrying out researches which are centered on marbling beef, lean beef and veal on complete techniques of good breed, feeding management, slaughter and cutting and carcass classification. At present, researchers have lively interest in functional genomics of meat quality traits of cattle, they expect to use these methods to study meat quality traits and then improve the meat quality. Improved cattle breeds universally utilized in China are mainly Simmental followed by Charolais, Limousin, Wagyu and Angus. Other three cattle breeds including Belgian blue cattle, Piedmontese and Gelbvien have ever been applied, however, rarely used in present beef cattle production. Republic of Serbia has in the future to quickly and efficiently provide adequate quantities of top quality meat. One of the ways to increase the yield and quality of the meat is crossing of Domestic spotted cattle of lower production traits with French beef cattle breeds. Beef production in the EU is adapted to the consumer taste. Meat must have a light red colour, equally suffused with fat and with pronounced sensory characteristics such as tenderness, juiciness, flavour and aroma. The results on the quality of meat of F1 generation crosses (Domestic Spotted breed with French beef cattle breeds Charolais and Limousine) indicate that by industrial crossing beef, meat-packing and organoleptic characteristics of meat can be improved.


2013 ◽  
Vol 56 (1) ◽  
pp. 315-327
Author(s):  
U. Sen ◽  
M. Kuran ◽  
U. Ensoy

Abstract. The aim of this study was to determine growth performance, carcass and meat quality of Karayaka female lambs born in different seasons. Winter born (breeding season; n=15) and autumn born (out of the breeding season; n=15) singleton female lambs were used in the study. Birth and slaughter weights of lambs in both groups were similar, but the weaning weight of the autumn born lambs was higher (P<0.05). The autumn born lambs had lower carcass yield, lung, spleen and gastrocnemious muscle weights (P<0.05), but higher liver, kidney, empty reticulo-rumen, empty small intestine, internal fat, suprarenal fat weights and a higher fat thickness over the longissimus dorsi muscle (P<0.05). Additionally, the winter born lambs had higher cooking loss, drip loss and shear force values (P<0.05), but lower dry matter and intramuscular fat (P<0.05) in longissimus dorsi and semitendinosus muscles. There were significant differences between both groups in terms of meat colour characteristics (P<0.05) except b* values for longissimus dorsi at 1 h and for semitendinosus at 24 h. The results suggest that Karayaka female lambs born in winter and autumn seasons have different meat quality parameters and growth patterns at pre- and post-weaning.


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