Association of Serum Bilirubin and Functional Variants of Heme Oxygenase 1 and Bilirubin UDP-Glucuronosyl Transferase Genes in Czech Adult Patients with Non-Alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disorder worldwide. The aim of our study was to assess the role of bilirubin, and the heme oxygenase 1 (HMOX1) and bilirubin UDP-glucuronosyl transferase (UGT1A1) promoter gene variants, which are involved in bilirubin homeostasis, in the NAFLD development in adult patients. The study was performed on 84 patients with NAFLD and 103 age/sex-matched controls. Routine biochemistry, inflammatory markers, adipokines, and the fibrosis/steatohepatitis stage were determined in the NAFLD patients. The (GT)n/(TA)n dinucleotide variations in HMOX1/UGT1A1 gene promoters, respectively, were analyzed by fragment analysis. Compared to controls, serum bilirubin concentrations in NAFLD patients tended to be decreased, while the prevalence of phenotypic Gilbert syndrome was significantly low. Genetic variations in HMOX1 and UGT1A1 gene promoters did not differ between NAFLD patients and controls, and no relationship was found in the NAFLD patients between these gene variants and any of the laboratory or histological parameters. In conclusion, metabolism of bilirubin is dysregulated in NAFLD patients, most likely due to increased oxidative stress, since frequencies of the major functional variants in the HMOX1 or UGT1A1 gene promoters did not have any effect on development of NAFLD in adult patients.

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PS-074 Heme Oxygenase-1 Gene Promoter Polymorphism And Insulin Resistance Are Associated With Susceptibility To Non-alcoholic Fatty Liver Disease In Children

Archives of Disease in Childhood ◽

10.1136/archdischild-2014-307384.371 ◽

2014 ◽

Vol 99 (Suppl 2) ◽

pp. A138.3-A139

Author(s):

P Chang ◽

Y Lin ◽

K Liu ◽

S Yeh

Keyword(s):

Insulin Resistance ◽

Liver Disease ◽

Heme Oxygenase ◽

Fatty Liver Disease ◽

Gene Promoter ◽

Promoter Polymorphism ◽

Heme Oxygenase 1 ◽

Alcoholic Fatty Liver ◽

Alcoholic Fatty Liver Disease ◽

Gene Promoter Polymorphism

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Association of Serum Bilirubin Level with Metabolic Syndrome and Non-Alcoholic Fatty Liver Disease: A Cross-Sectional Study of 1672 Obese Children

Journal of Clinical Medicine ◽

10.3390/jcm10132812 ◽

2021 ◽

Vol 10 (13) ◽

pp. 2812

Author(s):

Cristina Bellarosa ◽

Giorgio Bedogni ◽

Annalisa Bianco ◽

Sabrina Cicolini ◽

Diana Caroli ◽

...

Keyword(s):

Metabolic Syndrome ◽

Fatty Liver ◽

Fatty Liver Disease ◽

Serum Bilirubin ◽

Cross Sectional Study ◽

Sectional Study ◽

Obese Children ◽

Cross Sectional ◽

Alcoholic Fatty Liver ◽

Alcoholic Fatty Liver Disease

As in adults, obesity also plays a central role in the development of metabolic syndrome (MS) in children. Non-alcoholic fatty liver disease (NAFLD) is considered a manifestation of MS. Not only MS but also NAFLD seem to be inversely associated with serum bilirubin concentrations, an important endogenous tissue protector when only mild elevated. The aim of the study was to evaluate the association between serum bilirubin levels and the prevalence of MS and NAFLD in Italian obese children and adolescents. A retrospective cross-sectional study was performed in 1672 patients aged from 5 to 18 years. Clinical and laboratory parameters were assessed. NAFLD was measured by liver ultrasonography. The study was approved by the Ethical Committee of the Istituto Auxologico Italiano (research project code 1C021_2020, acronym BILOB). MS was present in 24% and fatty liver (FL) in 38% of this population. Bilirubin was not associated with FL and MS as a whole, but it was inversely associated only with selected components of MS, i.e., large WC, high blood pressure and high triglycerides. Our data suggest that bilirubin is not protective against MS and NAFLD in the presence of severe obesity.

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