scholarly journals Inhibition of Advanced Glycation End-Product Formation by High Antioxidant-Leveled Spices Commonly Used in European Cuisine

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 100 ◽  
Author(s):  
Małgorzata Starowicz ◽  
Henryk Zieliński
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antioxidant Capacity ◽  
Bovine Serum ◽  
Radical Scavenging ◽  
Model Systems ◽  
Total Phenolic ◽  
Advanced Glycation ◽  
Advanced Glycation End Product ◽  
Star Anise

Spices and herbs, as good sources of polyphenols, could be strong inhibitors of advanced glycation end-product (AGE) formation. The aim of this research was to measure the ability of various spices to inhibit AGEs and to study the correlation of AGE inhibition with total phenolic (TP) content and antioxidant capacity. Fourteen spices commonly used in European cuisine were extracted with a 50% ethanol solution, and their water and total phenolic contents and antioxidant capacities were examined. Antioxidant capacity was evaluated using three methods: (1) Measurement of the radical scavenging ability of 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and (2) 2,2-diphenyl-1-picrylhydrazyl (DPPH●); and (3) photochemiluminescence (PCL) assay. Antiglycation properties were studied in vivo using two model systems: Bovine serum albumin-glucose (BSA-glucose) and bovine serum albumin-methylglyoxal (BSA-MGO). The most potent glycation inhibitors, according to the BSA-MGO assay, were star anise (88%), cinnamon (85%), allspice (81%), and cloves (79%), whereas in the BSA-glucose measurement, oregano was noted to be a very effective inhibitor of the glycation process. The ability to inhibit glycation was highly correlated with TP values in the BSA-MGO and BSA-glucose assay (r = 0.84 and 0.76, respectively). Our research showed the high antiglycation ability of cinnamon, cloves, and allspice, and we suggest, for the first time, that anise could also be considered a good glycation inhibitor.

scholarly journals Evaluation of Anti-glycation Activities of Phlorotannins in Human and Bovine Serum Albumin-methylglyoxal Models

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Shingo Sugiura ◽  
Yuya Minami ◽  
Ryosuke Taniguchi ◽  
Reiji Tanaka ◽  
Hideo Miyake ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Positive Control ◽  
Ionization Mass ◽  
Advanced Glycation ◽  
Eisenia Arborea ◽  
Glycation End Products ◽  
End Products ◽  
Inhibitory Activities

In this study, the anti-glycation activities of phlorotannins contained in the Japanese Lessoniaceae ( Ecklonia cava, Eck. kurome, Eck. stolonifera, Eisenia arborea, and Eis. bicyclis) were tested using serum albumin-methlglyoxal (MGO) models. In the human serum albumin (HSA)-MGO model and the bovine serum albumin (BSA)-MGO model, the concentrations of crude phlorotannins at 50% inhibition (IC50) of fluorescent advanced glycation end products (AGEs) formation was in the range of 0.45 to 0.53 mg/mL and 0.43 to 0.53 mg/mL, respectively. Among the purified phlorotannins, phlorofucofuroeckol A with the benzobisbenzofuran skeleton had the highest inhibitory activities (IC50: 4.8 × 102 μM in HSA-MGO model and 4.1 × 102 μM in BSA-MGO model) against fluorescent AGEs formation, and showed about 13 times the inhibition in the HSA-MGO model, and about 20 times the inhibition in the BSA-MGO model, with respect to aminoguanidine hydrochloride, which was used as a positive control. In the test on MGO-scavenging activity using HPLC analysis, it showed that eckol and its simple dimers (dieckol and 8,8′-bieckol) had higher scavenging rate than aminoguanidine hydrochloride. In addition, it was revealed by liquid chromatography-electrospray ionization-mass spectrometry that eckol reacted with MGO and formed two adducts with it. These results strongly suggest that phlorotannins are novel and effective anti-glycation substances derived from natural plants.

scholarly journals Evaluation of Anti-glycation Activities of Phlorotannins in Human and Bovine Serum Albumin-glyceraldehyde Models

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Shingo Sugiura ◽  
Ryosuke Taniguchi ◽  
Yoshihiko Nishioka ◽  
Ryota Iwase ◽  
Reiji Tanaka ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Inhibitory Activity ◽  
Bovine Serum ◽  
Positive Control ◽  
Scavenging Activity ◽  
Advanced Glycation ◽  
Eisenia Arborea ◽  
Glycation End Products ◽  
End Products

The anti-glycation activities of phlorotannins contained in the Japanese Lessoniaceae ( Ecklonia cava, Eck. kurome, Eck. stolonifera, Eisenia arborea, and Eis. bicyclis) were tested using serum albumin-glyceraldehyde (GA) models. In the human serum albumin (HSA)-GA model and the bovine serum albumin (BSA)-GA model, the concentrations of crude phlorotannins at 50% inhibition (IC50) of fluorescent advanced glycation end products (AGEs) formation was in the range of 0.48 to 0.70 mg/mL and 0.52 to 0.75 mg/mL, respectively. In tests using phloroglucinol and purified phlorotannins (eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, and 8,8'-bieckol), dieckol had the highest inhibitory activity (IC50: 5.5 × 102 μM) against fluorescent AGEs formation in HSA-GA model and showed about 18 times inhibition compared with aminoguanidine hydrochloride of positive control. In the BSA albumin model, 8,8'-bieckol had about 27 times AGEs formation inhibitory activity (IC50: 6.2 × 102 μM) against aminoguanidine hydrochloride. In tests on GA scavenging activity, it was shown that compounds with phloroglucinol tetramer or higher had a scavenging rate of 70%, or more, with a reaction time of 120 minutes. These results suggest that among the phlorotannins, in particular the dimers of eckol (dieckol and 8,8'-bieckol), there are effective compounds for inhibiting the formation of AGEs derived from GA.

Impact of bovine serum albumin – A protein corona on toxicity of ZnO NPs in environmental model systems of plant, bacteria, algae and crustaceans

Chemosphere ◽  
2020 ◽  
pp. 128629
Author(s):  
B. Janani ◽  
Lija L. Raju ◽  
Ajith M. Thomas ◽  
Mohammed Nasser Alyemeni ◽  
Gani Asa Dudin ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Protein Corona ◽  
Model Systems ◽  
Zno Nps ◽  
Environmental Model

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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