scholarly journals p-Hydroxybenzoic Acid β-d-Glucosyl Ester and Cimidahurinine with Antimelanogenesis and Antioxidant Effects from Pyracantha angustifolia via Bioactivity-Guided Fractionation

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 258 ◽  
Author(s):  
Sun-Yup Shim ◽  
Ye Eun Lee ◽  
Hwa Young Song ◽  
Mina Lee
Keyword(s):  
Radical Scavenging ◽  
Ros Generation ◽  
Hydroxybenzoic Acid ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Melanin Production ◽  
B16f10 Cells ◽  
Tert Butyl Hydroperoxide ◽  
Glucosyl Ester ◽  
Dpph Radical Scavenging

This study evaluated bioactivity-guided fractionation as a means to identify therapeutic phytochemicals from Pyracantha angustifolia that can attenuate melanogenesis and oxidation. Seven compounds with inhibitory effects on melanin production and tyrosinase (TYR) activity, and ABTS and DPPH radical-scavenging activities, which have not been reported as whitening materials, were isolated from the n-butanol fraction from P. angustifolia leaves (PAL). Among the seven compounds, p-hydroxybenzoic acid β-d-glucosylester (HG), and cimidahurinine (CH) had strong inhibitory effects on melanin production and TYR activity, as well as ABTS and DPPH radical-scavenging activities. Western blot analysis showed that HG and CH suppressed tyrosinase-related protein (TYRP)-1 and TYRP-2 expression. Moreover, HG and CH inhibited reactive oxygen species (ROS) generation in tert-butyl hydroperoxide (t-BHP)-treated B16F10 cells. These results suggest that P. angustifolia containing active compounds, such as HG and CH, is a potent therapeutic candidate for the development of hypopigmenting agents.

scholarly journals p-Hydroxybenzoic Acid beta-D-Glucosyl Ester and Cimidahurinine with Antimelanogenesis and Antioxidant Effects from Pyracantha angustifolia via Bioactivity-Guided Fractionation

2020 ◽  
Author(s):  
Sun-Yup Shim ◽  
Ye Eun Lee ◽  
Hwa Young Song ◽  
Mina Lee
Keyword(s):  
Blood Circulation ◽  
Radical Scavenging ◽  
Hydroxybenzoic Acid ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Melanin Production ◽  
Glucosyl Ester ◽  
Dpph Radical Scavenging ◽  
Antioxidant Effects ◽  
Tyrosinase Related Protein

Pyracantha angustifolia has been used in traditional medicine to treat a range of diseases of the stomach and improve digestion, blood circulation, diarrhea, dysentery, and hemostasis. This study evaluated bioactivity-guided fractionation as a means to identify therapeutic phytochemicals from P. angustifolia that can attenuate melanogenesis and oxidation. Seven compounds with inhibitory effects on melanin production and tyrosinase (TYR) activity, as well as ABTS and DPPH radical scavenging activities, and have not been reported as whitening materials, were isolated from the n-butanol fraction from P. angustifolia leaves (PAL). Among the seven compounds, p-hydroxybenzoic acid beta-d-glucosylester (HG), and cimidahurinine (CD) had strong inhibitory effects of melanin production, TYR activity, and ABTS and DPPH radical scavenging activities. Western blot analysis showed that HG and CD suppressed tyrosinase-related protein (TYRP)-1 and TYRP-2 expression. These results suggest that P. angustifolia containing active compounds, such as HG and CH, is a potent therapeutic candidate for the development of hypopigmenting agents.

scholarly journals Antioxidant Compounds, Kirenol and Methyl ent-16α, 17-dihydroxy-kauran-19-oate Bioactivity-Guided Isolated from Siegesbeckia glabrescens Attenuates MITF-Mediated Melanogenesis via Inhibition of Intracellular ROS Production

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1940
Author(s):  
Sun-Yup Shim ◽  
Ye Eun Lee ◽  
Mina Lee
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Inhibitory Effects ◽  
Melanin Production ◽  
Mountainous Regions ◽  
B16f10 Cells ◽  
Tert Butyl Hydroperoxide ◽  
Melanoma B16f10 ◽  
Annual Herb

Siegesbeckia glabrescens (Compositae), an annual herb indigenous to Korean mountainous regions and has been eaten as a food in Korea. This study investigated ABTS, DPPH and nitric oxide (NO) radical-scavenging activities, and melanin production and TYR inhibitory effects-guided fractionation to identify therapeutic phytochemicals from S. glabrescens that can attenuate oxidation and melanogenesis in murine melanoma B16F10 cells. Nine compounds with inhibitory effects on melanin production, and TYR activity, and ABTS, DPPH, and NO radical scavenging activity were isolated from the 100% ethanol fraction from S. glabrescens. Among the nine compounds, kirenol (K), methyl ent-16α, 17-dihydroxy-kauran-19-oate (MDK) had strong inhibitory effects on melanin production and TYR activity with antioxidant effects. Western blot analysis revealed that K and MDK suppressed tyrosinase-related protein (TYRP)-1, TYRP-2 and microphthalmia-associated transcription factor (MITF) expression. Moreover, these two compounds inhibited intracellular reactive oxygen species (ROS) level in tert-butyl hydroperoxide (t-BHP)-treated B16F10 cells. Our results suggest that S. glabrescens containing active compounds such as K and MDK, which has antioxidant and antimelanogenesis effects, is the potent therapeutic and functional material for the prevention of oxidation-induced hyperpigmentation.

scholarly journals Isolation, Characterization and Antioxidant, Tyrosinase Inhibitory Activities of Constituents from the Flowers of Cercis glabra ‘Spring-1’

2021 ◽  
Vol 15 (4) ◽  
pp. 254-260
Author(s):  
Penghua Shu ◽  
Yamin Li ◽  
Yuehui Luo ◽  
Shujing Cai ◽  
Yingying Fei ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Mushroom Tyrosinase ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Isolation And Identification ◽  
Phytochemical Study ◽  
Inhibitory Activities ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

A phytochemical study on the flowers of Cercis glabra ‘Spring-1’ led to the isolation and identification of twelve compounds, including one new compound named as 1-O-α-l-rhamnosyl-(E)-phytol (1) and eleven known compounds. Their structures were elucidated based on physical data analysis, including HR-ESI-MS, NMR, UV, IR, and acid hydrolysis. All compounds were screened for in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl method. Compounds 4 and 5 exhibited obvious DPPH radical scavenging activities. All the isolates were tested for their inhibitory effects on mushroom tyrosinase, and compounds 6, 7, 10 and 11 showed moderate tyrosinase inhibitory activities.

scholarly journals The Use of Silica Extracted from Kaolin as Catalyst Support for Esterification of 4-Hydroxybenzoic Acid with Sucrose

2020 ◽  
Vol 23 (6) ◽  
pp. 196-202
Author(s):  
Iman Abdullah ◽  
Widayanti Wibowo ◽  
Soleh Kosela ◽  
Yuni Krisyuningsih Krisnandi
Keyword(s):  
Catalyst Support ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Solid Support ◽  
Esterification Reaction ◽  
Hydroxybenzoic Acid ◽  
Acid Catalysts ◽  
Dpph Radical ◽  
Xrd Pattern ◽  
Dpph Radical Scavenging

SiO2 was extracted from kaolin using concentrated HCl and HNO3 mixture and used as solid support for both Bronsted (HClO4 and H2SO4) and Lewis Acid (AlCl3 and ZnCl2). Before extraction, kaolin was calcined at 800°C for eight hours to form metastable kaolin. After the extraction procedure, silica was recovered in 51.6% yield. Silica and the resulting solid acids were then characterized using XRD, XRF, and FTIR. XRD pattern shows that silica synthesized from kaolin is in the mixture of amorphous and α-quartz silica. Immobilization of acids alters its structure becomes more crystalline. XRF analysis shows that the loading of AlCl3 and ZnCl2 was 9.5 and 24.8%, respectively. The acid catalysts were then used in the esterification reaction of 4-hydroxybenzoic acid with sucrose. The reactions were conducted in dimethyl sulfoxide (DMSO) solvent by varying reaction time and reaction temperature. The highest conversion of starting materials and selectivity towards ester was obtained over the AlCl3/SiO2 catalyst. DPPH radical scavenging test shows that the ester exhibits good antioxidant activity.

scholarly journals DPPH radical scavenging and lipoxygenase inhibitory effects in extracts from Erythrina senegalensis (Fabaceae) DC

2016 ◽  
Vol 10 (11) ◽  
pp. 185-191 ◽  
Author(s):  
Souleymane Fofana ◽  
Charlemagne Gnoula ◽  
Moussa Ou eacute draogo ◽  
Eloi Pal eacute ◽  
Roger HC N eacute bi eacute ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Dpph Radical Scavenging

Carum copticum L. essential oils as natural antimicrobial and antioxidant

2018 ◽  
Vol 44 (4) ◽  
pp. 515-520
Author(s):  
M Kazemi
Keyword(s):  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Carum Copticum ◽  
Microbial Strains ◽  
Pharmaceutical Industries ◽  
Dpph Radical Scavenging ◽  
Antioxidant Effects

Carum copticum L. comprises several relevant species for food, cosmetic, perfumery and pharmaceutical industries. GC/MS analysis of the enential oil of C. copticum revealed γ- terpinene as a major component of C. copticum, with its contribution of 33.85%. Essential oils (EOs) exhibited a significant antimicrobial activity against all tested microbial strains. In addition, the C. copticum oil demonstrated the highest DPPH radical scavenging activity. These results clearly show the antimicrobial and antioxidant effects of the plant essential oil.

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