scholarly journals Effect of Aeration Mode on Microbial Structure and Efficiency of Treatment of TSS-Rich Wastewater from Meat Processing

2020 ◽  
Vol 10 (21) ◽  
pp. 7414
Author(s):  
Piotr Jachimowicz ◽  
Agnieszka Cydzik-Kwiatkowska ◽  
Patrycja Szklarz

The present study investigated the effect of aeration mode on microbial structure and efficiency of treatment of wastewater with a high concentration of suspended solids (TSS) from meat processing in sequencing batch reactors (R). R1 was constantly aerated, while in R2 intermittent aeration was applied. DNA was isolated from biomass and analyzed using next-generation sequencing (NGS) and real-time PCR. As a result, in R1 aerobic granular sludge was cultivated (SVI30 = 44 mL g−1 MLSS), while in R2 a very well-settling mixture of aerobic granules and activated sludge was obtained (SVI30 = 65 mL g−1 MLSS). Intermittent aeration significantly increased denitrification and phosphorus removal efficiencies (68% vs. 43%, 73% vs. 65%, respectively) but resulted in decomposition of extracellular polymeric substances and worse-settling properties of biomass. In both reactors, microbial structure significantly changed in time; an increase in relative abundances of Arenimonas sp., Rhodobacterace, Thauera sp., and Dokdonella sp. characterized the biomass of stable treatment of meat-processing wastewater. Constant aeration in R1 cycle favored growth of glycogen-accumulating Amaricoccus tamworthensis (10.9%) and resulted in 2.4 times and 1.4 times greater number of ammonia-oxidizing bacteria and full-denitrifiers genes in biomass, respectively, compared to the R2.

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 386 ◽  
Author(s):  
Joanna Czarnota ◽  
Adam Masłoń ◽  
Monika Zdeb ◽  
Grzegorz Łagód

This study aimed to evaluate and compare the physical, chemical and biological properties of aerobic granular sludge from reactors with the addition of different powdered mineral materials. These properties have a significant impact on the efficiency of systems in which the biomass in granular form is used. Four identical granular sequencing batch reactors (GSBRs) were adopted for the research performed on a laboratory scale (R1—control reactor; R2, R3 and R4—with materials, PK, PG and PL respectively). The results indicate that the addition of powdered mineral materials improved the properties of biomass in reactors. The SVI5/SVI30 ratio values were significantly lower in the reactors with added materials (approx. 1.3 ± 0.3). The mean values of the sludge volume index at 30 min were the lowest in the R2 (39.8 ± 8.6 mL/g) and R4 (32.8 ± 10.7 mL/g) reactors. The settling velocity of biomass was the highest in the R2 reactor (15.4 ± 6.1 m/h). In the early days of the study, the highest extracellular polymeric substances (EPS) content was found in the biomass from the reactors to which the materials with higher Ca and Mg content were added (380.18–598.30 mg/g MLVSS). The rate of specific oxygen uptake (SOUR) by biomass indicated an insufficient biomass content in the R1 reactor—to 7.85 mg O2/(g MLVSS∙h)—while in the reactors with materials, the SOUR values were at the higher levels.


2015 ◽  
Vol 73 (3) ◽  
pp. 564-575 ◽  
Author(s):  
Jonas Margot ◽  
Samuel Lochmatter ◽  
D. A. Barry ◽  
Christof Holliger

Nitrifying wastewater treatment plants (WWTPs) are more efficient than non-nitrifying WWTPs to remove several micropollutants such as pharmaceuticals and pesticides. This may be related to the activity of nitrifying organisms, such as ammonia-oxidizing bacteria (AOBs), which could possibly co-metabolically oxidize micropollutants with their ammonia monooxygenase (AMO). The role of AOBs in micropollutant removal was investigated with aerobic granular sludge (AGS), a promising technology for municipal WWTPs. Two identical laboratory-scale AGS sequencing batch reactors (AGS-SBRs) were operated with or without nitrification (inhibition of AMOs) to assess their potential for micropollutant removal. Of the 36 micropollutants studied at 1 μg l−1 in synthetic wastewater, nine were over 80% removed, but 17 were eliminated by less than 20%. Five substances (bisphenol A, naproxen, irgarol, terbutryn and iohexol) were removed better in the reactor with nitrification, probably due to co-oxidation catalysed by AMOs. However, for the removal of all other micropollutants, AOBs did not seem to play a significant role. Many compounds were better removed in aerobic condition, suggesting that aerobic heterotrophic organisms were involved in the degradation. As the AGS-SBRs did not favour the growth of such organisms, their potential for micropollutant removal appeared to be lower than that of conventional nitrifying WWTPs.


2019 ◽  
Vol 131 ◽  
pp. 01129
Author(s):  
Xiaoying Zheng ◽  
Xiaoyao Shao ◽  
Yuan Zhang ◽  
Mengmeng Yang ◽  
Zhi Xu ◽  
...  

With the increasing use of cupric oxide nanoparticles (CuO NPs), its potential environmental toxicity has been concerned nowadays. Aerobic granular sludge (AGS) is a special collection of microorganisms. This research studied under long exposure to the concentration of 5, 10 and 20 mg/L of CuO NPs, pollutants removal efficiency of AGS, extracellular polymers (EPS) and microbial communities in aerobic/anaerobic/anoxic (A/O/A) sequencing batch reactors (SBRs). The results showed that COD removal rates was stable, and the removal efficiencies of TN decreased because of the high concentration CuO NPs. On the 45th day, the TP removal efficiency of the reactor with CuO NPs concentration of 10 mg/L and 20 mg/L decreased to 55.83% and 43.72%, respectively. The denitrifying phosphorus removal-aerobic granular sludge (DPR-AGS) had certain resistance to the short-term impact of CuO NPs, and the phosphorus removal ability decreased at the late stage of the impact test. Besides, CuO NPs decreased the stability of DPR-AGS. High-throughput sequencing showed that CuO NPs decreased microbial diversity of DPR-AGS.


Chemosphere ◽  
2020 ◽  
Vol 240 ◽  
pp. 124945 ◽  
Author(s):  
Yu Jiang ◽  
Yu Shang ◽  
Tengjing Gong ◽  
Zixin Hu ◽  
Kai Yang ◽  
...  

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