scholarly journals Effect of Semi-Continuous Anaerobic Digestion on the Substrate Solubilisation of Lignin-Rich Steam-Exploded Ludwigia grandiflora

2021 ◽  
Vol 11 (10) ◽  
pp. 4452
Author(s):  
Pranshu Bhatia ◽  
Masaaki Fujiwara ◽  
Maria Cecilia D. Salangsang ◽  
Jun Qian ◽  
Xin Liu ◽  
...  

In this study, semi-continuous anaerobic digestion of lignin-rich steam-exploded Ludwigia grandiflora (Lignin = 25.22% ± 4.6% total solids) was performed to understand better the effect of steam explosion on the substrate solubilisation and inhibitors formation during the process. Steam explosion pretreatment was performed at 180 °C for 30 min at a severity factor of 3.8 to enhance the biogas yield of the lignocellulosic biomass. The semi-continuous anaerobic digestion was performed in a continuously stirred tank reactor for 98 days at an initial hydraulic retention time of 30 days and an organic loading rate of 0.9 g-VS L−1day−1. The performed steam explosion pretreatment caused biomass solubilisation, resulting in enhanced biogas production during the process. During the anaerobic digestion process, the average biogas yield was 265 mL g-VS−1, and the pH throughout the operation was in the optimum range of 6.5–8.2. Due to fluctuations in the biogas yield, the hydraulic retention time and organic loading rate were changed on day 42 (50 days and 0.5 g-VS L−1day−1) and on day 49 (40 days and 0.7 g-VS L−1day−1), and 1 M of NaOH was added to the liquid fraction of the steam-exploded L. grandiflora during the latter part of the operation to maintain the stability in the reactor. Therefore, the steam explosion pretreatment helped in the degradation of L. grandiflora by breaking the lignocellulose structure. In addition, changes in the operating conditions of the anaerobic digestion led to an increase in the biogas production towards the end of the process, leading to the stability in the CSTR.

2011 ◽  
Vol 29 (11) ◽  
pp. 1171-1176 ◽  
Author(s):  
Thomas Schmidt

Oil production from Jatropha curcas L. seeds generates large amounts of Jatropha press cake (JPC) which can be utilized as a substrate for biogas production. The objective of this work was to investigate anaerobic mono-digestion of JPC and the effects of an iron additive (IA) on gas quality and process stability during the increase of the organic loading rate (OLR). With the increase of the OLR from 1.3 to 3.2 gVS L−1 day−1, the biogas yield in the reference reactor (RR) without IA decreased from 512 to 194 LN kgVS−1 and the CH4 concentration decreased from 69.3 to 44.4%. In the iron additive reactor (IAR), the biogas yield decreased from 530 to 462 LN kgVS−1 and the CH4 concentration decreased from 69.4 to 61.1%. The H2S concentration in the biogas was reduced by addition of the IA to values below 258 ppm in the IAR while H2S concentration in the RR increased and exceeded the detection limit of 5000 ppm. The acid capacity (AC) in the RR increased to more than 20 g L−1, indicating an accumulation of organic acids caused by process instability. AC values in the IAR remained stable at values below 5 g L−1. The results demonstrate that JPC can be used as sole substrate for anaerobic digestion up to an OLR of 2.4 gVS l−1 day−1. The addition of IA has effectively decreased the H2S content in the biogas and has improved the stability of the anaerobic process and the biogas quality.


2018 ◽  
Vol 7 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Agus Haryanto ◽  
Sugeng Triyono ◽  
Nugroho Hargo Wicaksono

The efficiency of biogas production in semi-continuous anaerobic digester is influenced by several factors, among other is loading rate. This research aimed at determining the effect of hydraulic retention time (HRT) on the biogas yield. Experiment was conducted using lab scale self-designed anaerobic digester of 36-L capacity with substrate of a mixture of fresh cow dung and water at a ratio of 1:1. Experiment was run with substrate initial amount of 25 L and five treatment variations of HRT, namely 1.31 gVS/L/d (P1), 2.47 gVS/L/d (P2), 3.82 gVS/L/d (P3), 5.35 gVS/L/d (P4) and 6.67 gVS/L/d (P5). Digester performance including pH, temperature, and biogas yield was measured every day. After stable condition was achieved, biogas composition was analyzed using a gas chromatograph. A 10-day moving average analysis of biogas production was performed to compare biogas yield of each treatment. Results showed that digesters run quite well with average pH of 6.8-7.0 and average daily temperature 28.7-29.1. The best biogas productivity (77.32 L/kg VSremoval) was found in P1 treatment (organic loading rate of 1.31 g/L/d) with biogas yield of 7.23 L/d. With methane content of 57.23% treatment P1 also produce the highest methane yield. Biogas production showed a stable rate after the day of 44. Modified Gompertz kinetic equation is suitable to model daily biogas yield as a function of digestion time.Article History: Received March 24th 2018; Received in revised form June 2nd 2018; Accepted June 16th 2018; Available onlineHow to Cite This Article: Haryanto, A., Triyono, S., and Wicaksono, N.H. (2018) Effect of Loading Rate on Biogas Production from Cow Dung in A Semi Continuous Anaerobic Digester. Int. Journal of Renewable Energy Development, 7(2), 93-100.https://doi.org/10.14710/ijred.7.2.93-100


Author(s):  
Napisa Pattharaprachayakul ◽  
Narumon Kesonlam ◽  
Pongpitak Duangjumpa ◽  
Vilai Rungsardthong ◽  
Worakrit Suvajittanont ◽  
...  

Pineapple wastes are produced in huge amount during the industrial canning process of pineapple; in Thailand over 400,000 tons per annum of canned pineapple exported leaving behind the waste. Besides the pulps and peels as solid wastes, the squeezed pineapple liquid wastes (SPLW) extracted from solid wastes can also be used for anaerobic digestion. In the present study, the anaerobic digestion of liquid squeezed from industrial pineapple peels was carried out using a lab-scale hybrid reactor. The reactor was operated for over 170 days with the hydraulic retention time (HRT) of 20 days decreasing down to 5 days and simultaneous control of organic loading rate (OLR). Under controlled conditions in the hybrid reactor, pH was maintained at 6.5–7.6 by adding alkaline for anaerobic microbial activity. Results showed that the chemical oxygen demand (COD) removal efficiency was at ≥ 90% for all conditions. The biogas production (mL/day) increased thoroughly from longer HRT to shorter HRT, as same as methane production with the maximum values (HRT 5 days, OLR 5 g/COD/ day with recirculation) of 55,130 and 30,322 mL/day, respectively. Moreover, the highest yields of biogas and methane were also investigated under similar conditions with the values of 0.504 and 0.277 L/gCOD, respectively. Interestingly, this optimization of both HRT and OLR of lab-scale anaerobic digestion process could be further practically applied to pilot or industrial scale in canned pineapple factories for biogas production.


2011 ◽  
Vol 64 (2) ◽  
pp. 320-325 ◽  
Author(s):  
J. Gustavsson ◽  
B. H. Svensson ◽  
A. Karlsson

The aim of this study was to investigate the effect of trace element supplementation on operation of wheat stillage-fed biogas tank reactors. The stillage used was a residue from bio-ethanol production, containing high levels of sulfate. In biogas production, high sulfate content has been associated with poor process stability in terms of low methane production and accumulation of process intermediates. However, the results of the present study show that this problem can be overcome by trace element supplementations. Four lab-scale wheat stillage-fed biogas tank reactors were operated for 345 days at a hydraulic retention time of 20 days (37 °C). It was concluded that daily supplementation with Co (0.5 mg L−1), Ni (0.2 mg L−1) and Fe (0.5 g L−1) were required for maintaining process stability at the organic loading rate of 4.0 g volatile solids L−1 day−1.


2020 ◽  
Author(s):  
Dejene Tsegaye Bedane ◽  
Mohammed Mazharuddin Khan ◽  
Seyoum Leta Asfaw

Abstract Background : Wastewater from agro-industries such as slaughterhouse is typical organic wastewater with high value of biochemical oxygen demand, chemical oxygen demand, biological organic nutrients (Nitrogen and phosphate) which are insoluble, slowly biodegradable solids, pathogenic and non-pathogenic bacteria and viruses, parasite eggs. Moreover it contains high protein and putrefies fast leading to environmental pollution problem. This indicates that slaughterhouses are among the most environmental polluting agro-industries. Anaerobic digestion is a sequence of metabolic steps involving consortiums of several microbial populations to form a complex metabolic interaction network resulting in the conversation of organic matter into methane (CH 4 ), carbon dioxide (CO 2 ) and other trace compounds. Separation of the phase permits the optimization of the organic loading rate and HRT based on the requirements of the microbial consortiums of each phase. The purpose of this study was to optimize the working conditions for the hydrolytic - acidogenic stage in two step/phase anaerobic digestion of slaughterhouse wastewater. The setup of the laboratory scale reactor was established at Center for Environmental Science, College of Natural Science with a total volume of 40 liter (36 liter working volume and 4 liter gas space). The working parameters for hydrolytic - acidogenic stage were optimized for six hydraulic retention time 1-6 days and equivalent organic loading rate of 5366.43 – 894.41 mg COD/L day to evaluate the effect of the working parameters on the performance of hydrolytic – acidogenic reactor. Result : The finding revealed that hydraulic retention time of 3 day with organic loading rate of 1,788.81 mg COD/L day was a as an optimal working conditions for the parameters under study for the hydrolytic - acidogenic stage. The degree of hydrolysis and acidification were mainly influenced by lower hydraulic retention time (higher organic loading rate) and highest values recorded were 63.92 % at hydraulic retention time of 3 day and 53.26% at hydraulic retention time of 2 day respectively. Conclusion : The finding of the present study indicated that at steady state the concentration of soluble chemical oxygen demand and total volatile fatty acids increase as hydraulic retention time decreased or organic loading rate increased from 1 day hydraulic retention time to 3 day hydraulic retention time and decreases as hydraulic retention time increase from 4 to 6 day. The lowest concentration of NH 4 + -N and highest degree of acidification was also achieved at hydraulic retention time of 3 day. Therefore, it can be concluded that hydraulic retention time of 3 day/organic loading rate of 1,788.81 mg COD/L .day was selected as an optimal working condition for the high performance and stability during the two stage anaerobic digestion of slaughterhouse wastewater for the hydrolytic-acidogenic stage under mesophilic temperature range selected (37.5℃). Keywords : Slaughterhouse Wastewater, Hydrolytic – Acidogenic, Two Phase Anaerobic Digestion, Optimal Condition, Agro-processing wastewater


Energies ◽  
2020 ◽  
Vol 13 (9) ◽  
pp. 2392 ◽  
Author(s):  
Marcin Dębowski ◽  
Marcin Zieliński ◽  
Marta Kisielewska ◽  
Joanna Kazimierowicz

The aim of this study was the performance evaluation of anaerobic digestion of dairy wastewater in a multi-section horizontal flow reactor (HFAR) equipped with microwave and ultrasonic generators to stimulate biochemical processes. The effects of increasing organic loading rate (OLR) ranging from 1.0 g chemical oxygen demand (COD)/L·d to 4.0 g COD/L·d on treatment performance, biogas production, and percentage of methane yield were determined. The highest organic compounds removals (about 85% as COD and total organic carbon—TOC) were obtained at OLR of 1.0–2.0 g COD/L·d. The highest biogas yield of 0.33 ± 0.03 L/g COD removed and methane content in biogas of 68.1 ± 5.8% were recorded at OLR of 1.0 g COD/L·d, while at OLR of 2.0 g COD/L·d it was 0.31 ± 0.02 L/COD removed and 66.3 ± 5.7%, respectively. Increasing of the OLR led to a reduction in biogas productivity as well as a decrease in methane content in biogas. The best technological effects were recorded in series with an operating mode of ultrasonic generators of 2 min work/28 min break. More intensive sonication reduced the efficiency of anaerobic digestion of dairy wastewater as well as biogas production. A low nutrient removal efficiency was observed in all tested series of the experiment, which ranged from 2.04 ± 0.38 to 4.59 ± 0.68% for phosphorus and from 9.67 ± 3.36 to 20.36 ± 0.32% for nitrogen. The effects obtained in the study (referring to the efficiency of wastewater treatment, biogas production, as well as to the results of economic analysis) proved that the HFAR can be competitive to existing industrial technologies for food wastewater treatment.


2018 ◽  
Vol 65 ◽  
pp. 05025 ◽  
Author(s):  
Sagor Kumar Pramanik ◽  
Fatihah Binti Suja ◽  
Biplob Kumar Pramanik ◽  
Shahrom Bindi Md Zain

Solid organic wastes create potential risks to environmental pollution and human health due to the uncontrolled discharge of huge quantities of hazardous wastes from numerous sources. Now-a-days, anaerobic digestion (AD) is considered as a verified and effective alternative compared to other techniques for treating solid organic waste. The paper reviewed the biological process and parameters involved in the AD along with the factors could enhance the AD process. Hydrolysis is considered as a rate-limiting phase in the complex AD process. The performance and stability of AD process is highly influenced by various operating parameters like temperature, pH, carbon and nitrogen ratio, retention time, and organic loading rate. Different pre-treatment (e.g. mechanical, chemical and biological) could enhance the AD process and the biogas yield. Co-digestion can also be used to provide suitable nutrient balance inside the digester. Challenges of the anaerobic digestion for biogas production are also discussed.


Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1487
Author(s):  
Vicky De Groof ◽  
Marta Coma ◽  
Tom C. Arnot ◽  
David J. Leak ◽  
Ana B. Lanham

Production of medium chain carboxylic acids (MCCA) as renewable feedstock bio-chemicals, from food waste (FW), requires complicated reactor configurations and supplementation of chemicals to achieve product selectivity. This study evaluated the manipulation of organic loading rate in an un-supplemented, single stage stirred tank reactor to steer an anaerobic digestion (AD) microbiome towards acidogenic fermentation (AF), and thence to chain elongation. Increasing substrate availability by switching to a FW feedstock with a higher COD stimulated chain elongation. The MCCA species n-caproic (10.1 ± 1.7 g L−1) and n-caprylic (2.9 ± 0.8 g L−1) acid were produced at concentrations comparable to more complex reactor set-ups. As a result, of the adjusted operating strategy, a more specialised microbiome developed containing several MCCA-producing bacteria, lactic acid-producing Olsenella spp. and hydrogenotrophic methanogens. By contrast, in an AD reactor that was operated in parallel to produce biogas, the retention times had to be doubled when fed with the high-COD FW to maintain biogas production. The AD microbiome comprised a diverse mixture of hydrolytic and acidogenic bacteria, and acetoclastic methanogens. The results suggest that manipulation of organic loading rate and food-to-microorganism ratio may be used as an operating strategy to direct an AD microbiome towards AF, and to stimulate chain elongation in FW fermentation, using a simple, un-supplemented stirred tank set-up. This outcome provides the opportunity to repurpose existing AD assets operating on food waste for biogas production, to produce potentially higher value MCCA products, via simple manipulation of the feeding strategy.


2011 ◽  
Vol 64 (3) ◽  
pp. 715-721 ◽  
Author(s):  
C. Cavinato ◽  
D. Bolzonella ◽  
F. Fatone ◽  
A. Giuliano ◽  
P. Pavan

This paper deals with the optimization of a two-phase anaerobic process treating biowaste for hydrogen and methane production. Neither physical nor chemical pre-treatments were used to optimize the process. The work was carried out at pilot scale, using two CSTRs (200 and 380 L working volume respectively) both maintained at thermophilic temperature (55 °C) and fed semi-continuously with biowaste. The experiment was divided into three periods; during the first two periods the organic loading rate was maintained at 20 kg TVS/m3 d and the hydraulic retention time was changed from 6.6 to 3.3 days, while in the last period the digestate of the second reactor was recirculated to the first reactor in order to buffer the system and control pH at levels around 5. The HRT was maintained at 3.3 days and the OLR was decreased at 16.5 kg TVS/m3 d. The best yield was obtained in the last period where a specific hydrogen production of 50.9 L/kg VSfed was reached, with a H2 content in biogas from the first reactor of 36%. The methanogenic stage after the hydrogen conversion reached a specific biogas production of 0.62 m3/kg VSfed and an overall organic removal above 70%, without any stability problem. The overall biogas production was some 1.5 m3 per day with a gas composition of 10% H2 and 50% CH4.


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