scholarly journals Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction

2021 ◽  
Vol 11 (16) ◽  
pp. 7419
Author(s):  
Pascaline Aimee Uwineza ◽  
Anna Gramza-Michałowska ◽  
Marcin Bryła ◽  
Agnieszka Waśkiewicz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Array Detector ◽  
Frap Assay ◽  
Constant Flow Rate ◽  
Lamium Album

In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent at a constant flow rate of CO2, methanol, and pressure. The collected extracts were characterized in terms of antioxidant capacity by using DPPH, ABTS and FRAP in vitro antioxidant activity assays, whereas the Folin–Ciocalteu procedure was employed to estimate the total phenols content (TPC). On the other hand, phenolic compounds in the extracts were quantitated by liquid chromatography coupled with a photodiode array detector (UPLC-PDA) and confirmed with a mass detector (TQD). The extracts have shown high TPC ranged between 234.17 to 650.17 mg GAE/g extract. DPPH scavenging of the extracts was estimated and obtained EC50 values ranged from 0.12 to 0.37 mg/mL of solution. The ABTS radical scavenging activity ranged from 43.20 to 44.53 µg TE/g. The FRAP value was found within the range of 19.48 to 44.74 µmol TE/g of extract. Differences between extraction conditions were observed. In this research, 50 °C/250 bar was efficient for the TPC, DPPH, ABTS, and FRAP assays; moreover, statistically, TPCs and FRAP assay showed significant differences between the conditions at α = 0.05. The identification of phenolic compounds in the obtained extract of Lamium album flowers, using UPLC/PDA, revealed that chrysin, pinostrobin, myricetin, and trans-3-hydroxycinnamic acid were the significant molecules present, which may be responsible for the high content of polyphenols and antioxidant activity. The results obtained indicated that SC-CO2 could be considered an alternative method for extracting bioactive compounds of Lamium album. High antioxidant activity and the presence of various bioactive compounds indicate the potential of this plant from the Lamiaceae family and the possibility of its application in various industries, including agriculture, food technology, or pharmacy.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

Phytochemical Investigation of Phenolic Constituents and in vitro Evaluation of Antioxidant Activity of Five Algerian Propolis

2020 ◽  
Vol 16 ◽  
Author(s):  
Amina Bouaroura ◽  
Narimane Segueni ◽  
Ramazan Erenler ◽  
Abdghani May ◽  
Chawki Bensouici ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phenolic Contents ◽  
Phenolic Constituents

Background:: Algerian propolis has gained interest in the last recent years. Many researches concerning both its biological effects and chemical composition were performed. Objective:: The present study was designed to investigate the chemical composition in particular phenolic compounds and the antioxidant activity of five Algerian propolis collected from different geographical parts of Algerian north namely: Constantine (CN), Boumerdes (BN), Mila (MN), Tebessa (TN) and Guelma (GN). Method:: Propolis extracts were obtained using solvents of varying polarity: petroleum ether, chloroform, ethyl acetate and finally methanol. Phenolic compounds were determined by liquid chromatography-tandem mass spectrometry (LCMS/ MS). In addition, total flavonoid and phenolic contents were also determined. Antioxidant activity was investigated using five complementary tests namely: DPPH., ABTS.+ assays for radical-scavenging activity, β-carotene-linoleic acid assay for lipid peroxidation activity, CUPRAC and FRAP assays for reduction capacity. Results:: The main phenolic compounds detected in the present study were: caffeic, p-coumaric, cinnamic and chlorogenic acids as well as naringenin and kampferol. Among the tested extracts, ethyl acetate and methanol extracts exhibited the highest phenolic and flavonoid contents and the strongest antioxidant activity. Propolis of Constantine was the most active one. Conclusion:: Our results suggest a potential use of Algerian propolis as a natural source of bioactive compounds with antioxidant activity.

scholarly journals Effect of the Addition of Propolis Extract on Bioactive Compounds and Antioxidant Activity of Craft Beer

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Pablo A. Ulloa ◽  
Juan Vidal ◽  
María I. Ávila ◽  
Mariela Labbe ◽  
Seth Cohen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Linear Increase ◽  
Total Phenolic ◽  
Frap Assay ◽  
Propolis Extract

Antioxidant-rich foods and beverages play an essential role in the prevention of diseases. This study assessed the influence of the addition of ethanolic extract of propolis (EEP) to beer at different concentrations (0.05, 0.15, and 0.25 g/L). Total phenolic content (TPC) and total flavonoid content (TFC) were determined. Antioxidant activity (AA) was evaluated by radical scavenging activity (DPPH and ABTS) and reducing power (FRAP). The addition of EEP in beer resulted in a linear increase in the TPC with values of 4.5%, 16.7%, and 26.7% above a control (no EEP added; 242 mg gallic acid equivalent/L). A similar increase was observed with TFC values 16.0%, 49.7%, and 59.2% above the control (16.9 mg quercetin equivalent/L). The FRAP assay indicated linear increases in AA relative to control with values of 1555, 1705, and 1892 μmol Trolox equivalent/L following EEP additions. The incorporation of EEP resulted in increases in the bioactive compounds and AA in beer without altering the physicochemical parameters of golden ale beer. The results indicate a promising use of propolis extract as a functional ingredient in beer.

scholarly journals ANTIOXIDANT ACTIVITY TEST OF FRACTIONS FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA

2020 ◽  
pp. 97-100
Author(s):  
ANNISA DHANIRA ◽  
BERNA ELYA ◽  
KATRIN BASAH
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Star Fruit

Objectives: Previous in vitro research showed that ethyl acetate fractions of star fruit leaves exhibit strong antioxidant activities toward 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC50 = 90 μg/ml). This study’s aim was to evaluate antioxidant activity of fractions from leaf extracts from star fruitsourced from three different regions in Indonesia (Depok, Sukabumi, and Subang) and, for the most active fraction (that with the highest antioxidantactivity), determine whether there was any correlation with phenolic and flavonoid content.Methods: Liquid–liquid partitioning, fractions were evaluated for in vitro antioxidant activity using DPPH radical scavenging and ferric reducingability of plasma (FRAP) assay.Results: The ethyl acetate fraction from the Subang region exhibited the strongest radical scavenging activity with both the DPPH assay (IC50 = 96 μg/ml)and the FRAP assay (FeEAC value = 1405 μmol/g).Conclusion: Ethyl acetate fractions from star fruit leaves could be used as natural antioxidants. No correlation was found between antioxidant activityand phenolic or flavonoid content.

scholarly journals A study on in vitro antioxidant activity of aqueous seed extract of sesbania sesban (l) merr.

2021 ◽  
Vol 8 (2) ◽  
pp. 69-74
Author(s):  
Kathiravan S ◽  
Shwetha V Kalava
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Seed Extract ◽  
Dependent Manner ◽  
Sesbania Sesban ◽  
Frap Assay ◽  
Increase With Increase ◽  
In Vitro Antioxidant Activity

The present study was done to investigate the in vitro antioxidant activity of aqueous extract of Sesbania sesban seeds. The assays such as DPPH, Chelation, ferrous ion, ABTS, Superoxide radical, hydroxyl radical assay, FRAP assay and total antioxidant activity were done to assess the antioxidant potential of the seed extract. The extract was tested at a concentration range of 100 – 500 μg/ml for all the assays and the values were compared with a standard. The results obtained showed that the radical scavenging activity was in a dose dependent manner and found to increase with increase in concentration of the extract. The IC50 value was calculated for the assays and tabulated for inference. Different assays revealed different levels of radical scavenging potential of the extract and exhibited as a better antioxidant source for therapeutic applications.

scholarly journals In Vitro Antioxidant Activities of Three Selected Dates from Tunisia (Phoenix dactyliferaL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Wissal Kchaou ◽  
Fatma Abbès ◽  
Hamadi Attia ◽  
Souhail Besbes
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Food Additives ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Frap Assay

Second-grade dates (Phoenix dactyliferaL.), with hard texture, from three selected Tunisian cultivars (Allig, Deglet Nour, and Bejo) were analysed from their antioxidant activities using DPPH radical scavenging activity, FRAP assay, H2O2scavenging activity, and metal chelating activity. Date extracts showed strong and concentration-dependant activity in all tested methods. The results showed that the best antioxidant activity was obtained in Allig, followed by Bejo and Deglet Nour. Total phenolics, total flavonoids, carotenoids, and tannins were determined spectrophotometrically in three date extracts. Results indicated that date contained significantly different amounts of these compounds. In fact, Allig presented the highest antioxidant compounds, followed by Bejo and Deglet Nour. Correlation analyses indicated a positive linear relationship between antioxidant activities and total phenolic and flavonoid content of date. This study demonstrates the potential antioxidant activity with Tunisian date, where we can use these natural extracts as food additives in replacement of synthetic compounds.

scholarly journals In Vitro Antioxidant Activity of Sesame Milk Fermentation in Human Low Density Lipoprotein (LDL)

2019 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sesame Seed ◽  
Hydrolysis Rate ◽  
Ldl Oxidation ◽  
Array Detector ◽  
Glucosidase Activity ◽  
In Vitro Antioxidant Activity

Lignan sesaminol triglucoside is a bioactive compound in sesame milk that showed higher antioxidant activity after it is hydrolyzed by β-glucosidase. The objectives of this study were to determine in vitro antioxidant activity of fermented sesame milk (FSM) extract and sesame milk (SM) extract against DPPH and LDL oxidation and to examine phenolic, sesaminol triglucoside content and β-glucosidase activity. Antioxidant activity was examined using DPPH and TBARS assay with LDL as the oxidation substrate. Sesaminol triglucoside was identified with HPLC diode array detector and β-glucosidase activity was determined by measuring hydrolysis rate of ρ-nitrophenyl-β-D-glucopyranoside (ρNPG). After fermentation of sesame milk with L. plantarum Dad 13, the β-glucosidase activity was 70.3±0.023 mU/mL fermented sesame milk, the sesaminol triglucoside content of SM and FSM were 5.65 to 2.56 mg/100 mL of sesame milk, respectively, the phenolic content of SM and FSM were 3.81±0.10 and 7.9±0.08 mg GAE/g dry sesame seed, respectively, radical scavenging activity of SM and FSM 18±0.64, and 45.5±0.37% respectively. Fermented sesame milk inhibited human plasma LDL oxidation by 1.82 compared to unfermented sesame milk. This result related to hydrolysis of sesaminol triglucoside by β-glucosidase which was produced by L. plantarum Dad 13 that resulted in sesaminol. This results suggest that fermented sesame milk extract has in vitro antioxidant activity in human LDL better than sesame milk extract

scholarly journals In-vitro Antioxidant and Anti-lipid Peroxidation Activity of Ethanolic Extracts of Bougainvillea shubhra, Bougainvillea peruviana and Bougainvillea bhuttiana Golden Glow: A Comparative Study

2015 ◽  
Vol 15 (1) ◽  
pp. 43 ◽  
Author(s):  
Mosmi Medpilwar ◽  
Darshil Maru ◽  
Meenakshi Upadhyay ◽  
Neha Lavania ◽  
Madhavi Vernekar ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Frap Assay ◽  
Ethanolic Extracts ◽  
In Vitro Inhibition ◽  
Reducing Power Assay

The aim of our present study was to evaluate the antioxidant and anti-lipid peroxidation activity of ethanolic extracts of <em>Bougainvillea species viz B. shubhra, B. peruviana and B. bhuttiana</em> golden glow. Phytochemical constituents viz. total phenolics, flavonoids and tannins were assayed using standard protocol. The antioxidant activity was studied by DPPH assay, FRAP assay, reducing power assay and in-vitro inhibition of lipid peroxidation. Of the three species, <em>B. bhuttiana</em> golden glow showed highest amount of phenols (6.78±0.001), flavonoids (27.7±0.012) and tannin (11.08±0.008) contents. It also showed highest antioxidant activity of 15.15± 0.008 and 8.08± 0.018 as determined by FRAP assay and reducing power assay respectively. The percent DPPH radical scavenging activity was 82.72% and in-vitro inhibition of lipid peroxidation in mitochondrial membrane was also found to be highest in <em>B. bhuttiana</em> golden glow.

Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

2020 ◽  
Vol 16 ◽  
Author(s):  
Sajjad Esmaeili ◽  
Nazanin Ghobadi ◽  
Donya Nazari ◽  
Alireza Pourhossein ◽  
Hassan Rasouli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibitor ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Curcumin Derivatives ◽  
Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

scholarly journals The Effects of Bioactive Compounds from Blueberry and Blackcurrant Powder on Oat Bran Pastes: Enhancing In Vitro Antioxidant Activity and Reducing Reactive Oxygen Species in Lipopolysaccharide-Stimulated Raw264.7 Macrophages

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 388
Author(s):  
Xiao Dan Hui ◽  
Gang Wu ◽  
Duo Han ◽  
Xi Gong ◽  
Xi Yang Wu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Raw264.7 Macrophages ◽  
Oat Bran

In this study, blueberry and blackcurrant powder were chosen as the phenolic-rich enrichments for oat bran. A Rapid Visco Analyser was used to form blueberry and blackcurrant enriched oat pastes. An in vitro digestion process evaluated the changes of phenolic compounds and the in vitro antioxidant potential of extracts of pastes. The anthocyanidin profiles in the extracts were characterised by the pH differential method. The results showed that blueberry and blackcurrant powder significantly increased the content of phenolic compounds and the in vitro antioxidant capacity of pastes, while the total flavonoid content decreased after digestion compared to the undigested samples. Strong correlations between these bioactive compounds and antioxidant values were observed. Lipopolysaccharide-stimulated RAW264.7 macrophages were used to investigate the intracellular antioxidant activity of the extracts from the digested oat bran paste with 25% enrichment of blueberry or blackcurrant powder. The results indicated that the extracts of digested pastes prevented the macrophages from experiencing lipopolysaccharide (LPS)-stimulated intracellular reactive oxygen species accumulation, mainly by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signalling pathway. These findings suggest that the bioactive ingredients from blueberry and blackcurrant powder enhanced the in vitro and intracellular antioxidant capacity of oat bran pastes, and these enriched pastes have the potential to be utilised in the development of the functional foods.

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