The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica

Our present study concerns the influence of the picosecond rise-time-pulsed electromagnetic field, and the impact of nanosecond pulsed pressure on the aggregation state of horseradish peroxidase (HRP) as a model enzyme. The influence of a 640 kV/m pulsed electromagnetic field with a pulse rise-time of ~200 ps on the activity and aggregation state of an enzyme is studied by the single-molecule atomic force microscopy (AFM) method. The influence of such a field is shown to lead to aggregation of the protein and to a decrease in its enzymatic activity. Moreover, the effect of a shock wave with a pressure front rise-time of 80 ns on the increase in the HRP aggregation is demonstrated. The results obtained herein can be of use in modeling the impact of electromagnetic and pressure pulses on enzymes and on whole living organisms. Our results are also important for taking into account the effect of pulsed fields on the body in the development of drugs, therapeutic procedures, and novel highly sensitive medical diagnosticums.

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The impact of a pulsed electromagnetic field on the seed protein content of soybean

Journal of Agricultural Sciences Belgrade ◽

10.2298/jas2004311d ◽

2020 ◽

Vol 65 (4) ◽

pp. 311-320

Author(s):

Vojin Djukic ◽

Zlatica Miladinov ◽

Gordana Dozet ◽

Gorica Cvijanovic ◽

Jegor Miladinovic ◽

...

Keyword(s):

Electromagnetic Field ◽

Plant Breeding ◽

Exposure Time ◽

Pulse Generator ◽

Soybean Seed ◽

Organic Production ◽

Efficient Production ◽

Pulsed Electromagnetic Field ◽

Pulsed Electromagnetic ◽

The Impact

Many studies show that in the last 20 years, the increase in production per hectare has been achieved mainly due to the creation of new varieties and the development of plant breeding. Innovations in plant breeding are the main way to find new traits, values and tolerances, which are the only ones that can respond to the increased demand for yield and more efficient production. In addition to high and stable yields, it is very important that the soybean seed has a satisfactory technological quality. Therefore, the aim of this study was to examine how the application of pulsed electromagnetic fields (PEMFs) affected the protein yield of soybean seed depending on the year, exposure time and frequency. The field trials were conducted in the experimental field of the Institute of Field and Vegetable Crops in Novi Sad, Serbia in 2010-2013. For this research, the seeds of a medium-sized variety Valjevka exposed to a pulsed electromagnetic field (PEMF) using a pulse generator and a strip applicator were used. A low frequency pulsed electromagnetic field (16, 24, 30, 72 Hz) was used at the exposure times of 0, 30, 60, and 90 minutes. The results of the research show that the application of this method can increase the yield of protein in soybean seed for more than 20%, which is a significant increase, especially in organic production, where the use of seed treatment agents is very restricted. However, this measure can also have an inhibitory effect if an adverse combination of exposure time and frequency strength is selected.

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Abstract #283 Management of Diabetic Peripheral Neuropathy (DPN) Using Low Frequency Pulsed Electromagnetic Field (LF-PEMF) Post Unsatisfactory Pharmacological Treatment.

Endocrine Practice ◽

10.1016/s1530-891x(20)47128-9 ◽

2018 ◽

Vol 24 ◽

pp. 73-74

Author(s):

Satish Wasoori ◽

Chetan Bhardwaj ◽

Arsh Wasoori

Keyword(s):

Electromagnetic Field ◽

Peripheral Neuropathy ◽

Pharmacological Treatment ◽

Diabetic Peripheral Neuropathy ◽

Low Frequency ◽

Pulsed Electromagnetic Field ◽

Pulsed Electromagnetic

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02 / Antimicrobial effects of pulsed electromagnetic field: in-vitro polymicrobial periodontal subgingival biofilm model

10.26226/morressier.5ac3832d2afeeb00097a3f13 ◽

2018 ◽

Author(s):

Jamil A. Shibli

Keyword(s):

Electromagnetic Field ◽

Pulsed Electromagnetic Field ◽

Antimicrobial Effects ◽

Biofilm Model ◽

Pulsed Electromagnetic

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Influence of pulsed electromagnetic field treatment on stress-strain state of circumferential welded joints of aluminium АМg6 alloy

The Paton Welding Journal ◽

10.37434/tpwj2020.10.02 ◽

2020 ◽

Vol 2020 (10) ◽

pp. 7-12

Author(s):

L.M. Lobanov ◽

◽

M.O. Pashchin ◽

O.L. Mikhodui ◽

O.V. Cherkashin ◽

...

Keyword(s):

Electromagnetic Field ◽

Welded Joints ◽

Strain State ◽

Pulsed Electromagnetic Field ◽

Stress Strain ◽

Stress Strain State ◽

Pulsed Electromagnetic

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Pulsed electromagnetic field stimulation may improve fusion rates in cervical arthrodesis in high-risk populations

Bone and Joint Research ◽

10.1302/2046-3758.72.bjr-2017-0221.r1 ◽

2018 ◽

Vol 7 (2) ◽

pp. 124-130 ◽

Cited By ~ 3

Author(s):

D. Coric ◽

D. E. Bullard ◽

V. V. Patel ◽

J. T. Ryaby ◽

B. L. Atkinson ◽

...

Keyword(s):

Electromagnetic Field ◽

Risk Factor ◽

High Risk ◽

Clinical Risk Factor ◽

Field Stimulation ◽

Pulsed Electromagnetic Field ◽

Exact Test ◽

High Risk Populations ◽

Pulsed Electromagnetic ◽

Pemf Treatment

Objectives Pulsed electromagnetic field (PEMF) stimulation was evaluated after anterior cervical discectomy and fusion (ACDF) procedures in a randomized, controlled clinical study performed for United States Food and Drug Administration (FDA) approval. PEMF significantly increased fusion rates at six months, but 12-month fusion outcomes for subjects at elevated risk for pseudoarthrosis were not thoroughly reported. The objective of the current study was to evaluate the effect of PEMF treatment on subjects at increased risk for pseudoarthrosis after ACDF procedures. Methods Two evaluations were performed that compared fusion rates between PEMF stimulation and a historical control (160 subjects) from the FDA investigational device exemption (IDE) study: a post hoc (PH) analysis of high-risk subjects from the FDA study (PH PEMF); and a multicentre, open-label (OL) study consisting of 274 subjects treated with PEMF (OL PEMF). Fisher’s exact test and multivariate logistic regression was used to compare fusion rates between PEMF-treated subjects and historical controls. Results In separate comparisons of PH PEMF and OL PEMF groups to the historical control group, PEMF treatment significantly (p < 0.05, Fisher’s exact test) increased the fusion rate at six and 12 months for certain high-risk subjects who had at least one clinical risk factor of being elderly, a nicotine user, osteoporotic, or diabetic; and for those with at least one clinical risk factor and who received at least a two- or three-level arthrodesis. Conclusion Adjunctive PEMF treatment can be recommended for patients who are at high risk for pseudoarthrosis. Cite this article: D. Coric, D. E. Bullard, V. V. Patel, J. T. Ryaby, B. L. Atkinson, D. He, R. D. Guyer. Pulsed electromagnetic field stimulation may improve fusion rates in cervical arthrodesis in high-risk populations. Bone Joint Res 2018;7:124–130. DOI: 10.1302/2046-3758.72.BJR-2017-0221.R1.

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