scholarly journals Mass Spectrometry Imaging Analysis of Metabolic Changes in Green and Red Tomato Fruits Exposed to Drought Stress

2021 ◽  
Vol 12 (1) ◽  
pp. 216
Author(s):  
Hiroko Asakura ◽  
Shu Taira ◽  
Junko Funaki ◽  
Takashi Yamakawa ◽  
Keiko Abe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Drought Stress ◽  
Mass Spectrometry Imaging ◽  
Environmental Changes ◽  
Vegetable Crops ◽  
Imaging Analysis ◽  
Tomato Fruits ◽  
Physiological Processes ◽  
Drought Conditions

Plant metabolism is altered in response to various environmental changes. In vegetable crops such as tomato (Solanum lycopersicum), the metabolic composition of fruits varies depending on the variety or cultivar as well as the cultivation method used. Few studies have examined the metabolic fluctuations in fruits under stress conditions, such as drought. We previously examined the metabolomes of mature green tomato fruits, which undergo drastic changes in chemical composition during ripening, and mature red fruits in response to drought stress. We detected or predicted fluctuations in the levels of fatty acids and phospholipid constituents, such as inositol and ethanolamine. In this study, we determined the localizations of these metabolites in fruits using mass spectrometry imaging. The accumulation patterns of stearic acid and palmitic acid were similar, but unlike these fatty acids, oleic acid accumulated to high levels in the placenta. Inositol is involved in various physiological processes; under drought conditions, this metabolite is synthesized by a different pathway compared to under normal conditions. The biosynthesis of pectin, a component of the gel surrounding the seeds, was suppressed under drought stress but increased in seeds. We propose that under drought conditions, a shift to phospholipid biosynthesis occurs that protects seeds from dehydration.

scholarly journals Transcriptomic, peptidomic and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus

2021 ◽  
Author(s):  
Jens Habenstein ◽  
Franziska Schmitt ◽  
Sander Liessem ◽  
Alice Ly ◽  
Dennis Trede ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Imaging Analysis ◽  
The Brain

scholarly journals MALDI-TOF/TOF Tandem Mass Spectrometry Imaging Reveals Non-uniform Distribution of Disaccharide Isomers in Plant Tissues

2020 ◽  
Author(s):  
lingpeng zhan ◽  
xi huang ◽  
jinjuan xue ◽  
huihui liu ◽  
caiqiao xiong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Biological Tissues ◽  
Plant Tissues ◽  
Tandem Mass ◽  
Imaging Analysis ◽  
Maldi Tof ◽  
Ms Imaging ◽  
Imaging Approach

Mass spectrometry imaging (MSI) has been increasingly utilized in investigating the locations of biomolecules within tissues. However, the isomeric compounds are rarely distinguished in the MS images, due to inability of MSI methods to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds in ion images. Here we apply MALDI-TOF/TOF tandem mass spectrometry imaging approach to revealing the spatial distributions of isomeric disaccharides in plant tissues. First, the MS/MS imaging analysis of disaccharide-matrix droplet spots demonstrated the feasibility of distinguishing isomeric species in tissues, by measuring the relative intensity of specific fragments. Then, we conducted tandem MS imaging of disaccharides in onion bulb tissues, which indicated that sucrose and other unknown non-sucrose disaccharides exhibit heterogeneous locations throughout the tissues. This method enables us to image disaccharide isomers differentially in biological tissues, and to discover new saccharide species in plant. This work also emphasizes the necessity of considering isobaric compounds when interpreting MSI results.<br>

scholarly journals Raman2imzML converts Raman imaging data into the standard mass spectrometry imaging format

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Stefania Alexandra Iakab ◽  
Lluc Sementé ◽  
María García-Altares ◽  
Xavier Correig ◽  
Pere Ràfols
Keyword(s):  
Mass Spectrometry ◽  
Open Source ◽  
Open Source Software ◽  
Multimodal Imaging ◽  
Mass Spectrometry Imaging ◽  
Raman Imaging ◽  
Imaging Analysis ◽  
Imaging Data ◽  
Data Format ◽  
Computational Tools

Abstract Background Multimodal imaging that combines mass spectrometry imaging (MSI) with Raman imaging is a rapidly developing multidisciplinary analytical method used by a growing number of research groups. Computational tools that can visualize and aid the analysis of datasets by both techniques are in demand. Results Raman2imzML was developed as an open-source converter that transforms Raman imaging data into imzML, a standardized common data format created and adopted by the mass spectrometry community. We successfully converted Raman datasets to imzML and visualized Raman images using open-source software designed for MSI applications. Conclusion Raman2imzML enables both MSI and Raman images to be visualized using the same file format and the same software for a straightforward exploratory imaging analysis.

scholarly journals MALDI-TOF/TOF Tandem Mass Spectrometry Imaging Reveals Non-uniform Distribution of Disaccharide Isomers in Plant Tissues

2020 ◽  
Author(s):  
lingpeng zhan ◽  
xi huang ◽  
jinjuan xue ◽  
huihui liu ◽  
caiqiao xiong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Biological Tissues ◽  
Plant Tissues ◽  
Tandem Mass ◽  
Imaging Analysis ◽  
Maldi Tof ◽  
Ms Imaging ◽  
Imaging Approach

Mass spectrometry imaging (MSI) has been increasingly utilized in investigating the locations of biomolecules within tissues. However, the isomeric compounds are rarely distinguished in the MS images, due to inability of MSI methods to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds in ion images. Here we apply MALDI-TOF/TOF tandem mass spectrometry imaging approach to revealing the spatial distributions of isomeric disaccharides in plant tissues. First, the MS/MS imaging analysis of disaccharide-matrix droplet spots demonstrated the feasibility of distinguishing isomeric species in tissues, by measuring the relative intensity of specific fragments. Then, we conducted tandem MS imaging of disaccharides in onion bulb tissues, which indicated that sucrose and other unknown non-sucrose disaccharides exhibit heterogeneous locations throughout the tissues. This method enables us to image disaccharide isomers differentially in biological tissues, and to discover new saccharide species in plant. This work also emphasizes the necessity of considering isobaric compounds when interpreting MSI results.<br>

scholarly journals MALDI-TOF/TOF Tandem Mass Spectrometry Imaging Reveals Non-uniform Distribution of Disaccharide Isomers in Plant Tissues

2020 ◽  
Author(s):  
lingpeng zhan ◽  
xi huang ◽  
jinjuan xue ◽  
huihui liu ◽  
caiqiao xiong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Biological Tissues ◽  
Plant Tissues ◽  
Tandem Mass ◽  
Imaging Analysis ◽  
Maldi Tof ◽  
Ms Imaging ◽  
Imaging Approach

Mass spectrometry imaging (MSI) has been increasingly utilized in investigating the locations of biomolecules within tissues. However, the isomeric compounds are rarely distinguished in the MS images, due to inability of MSI methods to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds in ion images. Here we apply MALDI-TOF/TOF tandem mass spectrometry imaging approach to revealing the spatial distributions of isomeric disaccharides in plant tissues. First, the MS/MS imaging analysis of disaccharide-matrix droplet spots demonstrated the feasibility of distinguishing isomeric species in tissues, by measuring the relative intensity of specific fragments. Then, we conducted tandem MS imaging of disaccharides in onion bulb tissues, which indicated that sucrose and other unknown non-sucrose disaccharides exhibit heterogeneous locations throughout the tissues. This method enables us to image disaccharide isomers differentially in biological tissues, and to discover new saccharide species in plant. This work also emphasizes the necessity of considering isobaric compounds when interpreting MSI results.<br>

scholarly journals The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jiabin Shen ◽  
Bo Lv ◽  
Liqiong Luo ◽  
Jianmei He ◽  
Chanjuan Mao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Environmental Changes ◽  
Abiotic Stress Tolerance ◽  
Late Embryogenesis Abundant ◽  
High Salt ◽  
Marker Genes ◽  
Drought Conditions

Abstract Plants can perceive environmental changes and respond to external stressors. Here, we show that OsNAC2, a member of the NAC transcription factor family, was strongly induced by ABA and osmotic stressors such as drought and high salt. With reduced yields under drought conditions at the flowering stage, OsNAC2 overexpression lines had lower resistance to high salt and drought conditions. RNAi plants showed enhanced tolerance to high salinity and drought stress at both the vegetative and flowering stages. Furthermore, RNAi plants had improved yields after drought stress. A microarray assay indicated that many ABA-dependent stress-related genes were down-regulated in OsNAC2 overexpression lines. We further confirmed that OsNAC2 directly binds the promoters of LATE EMBRYOGENESIS ABUNDANT 3 (OsLEA3) and Stress-Activated Protein Kinases 1 (OsSAPK1), two marker genes in the abiotic stress and ABA response pathways, respectively. Our results suggest that in rice OsNAC2 regulates both abiotic stress responses and ABA-mediated responses, and acts at the junction between the ABA and abiotic stress pathways.

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