scholarly journals The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jiabin Shen ◽  
Bo Lv ◽  
Liqiong Luo ◽  
Jianmei He ◽  
Chanjuan Mao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Environmental Changes ◽  
Abiotic Stress Tolerance ◽  
Late Embryogenesis Abundant ◽  
High Salt ◽  
Marker Genes ◽  
Drought Conditions

Abstract Plants can perceive environmental changes and respond to external stressors. Here, we show that OsNAC2, a member of the NAC transcription factor family, was strongly induced by ABA and osmotic stressors such as drought and high salt. With reduced yields under drought conditions at the flowering stage, OsNAC2 overexpression lines had lower resistance to high salt and drought conditions. RNAi plants showed enhanced tolerance to high salinity and drought stress at both the vegetative and flowering stages. Furthermore, RNAi plants had improved yields after drought stress. A microarray assay indicated that many ABA-dependent stress-related genes were down-regulated in OsNAC2 overexpression lines. We further confirmed that OsNAC2 directly binds the promoters of LATE EMBRYOGENESIS ABUNDANT 3 (OsLEA3) and Stress-Activated Protein Kinases 1 (OsSAPK1), two marker genes in the abiotic stress and ABA response pathways, respectively. Our results suggest that in rice OsNAC2 regulates both abiotic stress responses and ABA-mediated responses, and acts at the junction between the ABA and abiotic stress pathways.

scholarly journals Histone Deacetylase Gene SlHDA3 Involves in ABA, Drought and Salt Response in Tomato 

2021 ◽  
Author(s):  
Jun-E Guo
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Histone Deacetylase ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Histone Deacetylation ◽  
New Members ◽  
Modifying Factors ◽  
Mda Content

Abstract Histone deacetylation, one of vital modifying factors of post-translation modifications, which is catalyzed by histone deacetylase. The genes of histone deacetylase(HDACs) play critical roles in various stress responses. However, detailed functions for most SlHDAC members in tomato still unknown. In this work, we found that a histone deacetylase, SlHDA3, involved in response to NaCl and drought abiotic stresses. The expression of SlHDA3 was also induced significantly by NaCl, drought stress and endogenous hormone treatments. Silencing of SlHDA3 in tomato, the RNAi transgenic plants presented depressed tolerance to drought and salt stresses compared with WT tomato. The results of sensitivity analysis indicated that the length of hypocotyl and roots in RNAi plants were more inhibited by ABA and salt stress than that of WT at post-germination stage. Worse growth status were exhibited in SlHDA3 transgenic plants under salt and drought stress as are evaluated by a series of physiological parameters related to stress responses, such as decreased RWC, survival rate, ABA content, chlorophyll content and CAT activity, and increased MDA content and proline content. Besides, the expressions analysis of transgenic plants showed that the transcripts of genes which associated with responses to abiotic stress were down-regulated under salt-stressed conditions. To sum up, SlHDA3 acts as a stress-responsive gene, plays a role in the positive regulation of abiotic stress tolerance, and may be one of the new members in the engineering breeding of salt- and drought-tolerant tomato.

scholarly journals A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant

2020 ◽  
Vol 21 (13) ◽  
pp. 4701
Author(s):  
Qing He ◽  
Hanyang Cai ◽  
Mengyan Bai ◽  
Man Zhang ◽  
Fangqian Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Pseudomonas Syringae ◽  
Stress Responses ◽  
Stomatal Closure ◽  
Negative Regulator ◽  
Marker Genes ◽  
Genes Expression ◽  
Salt And Drought Stress ◽  
Salt And Drought Stresses

The basic leucine zipper (bZIP) is a plant-specific transcription factor family that plays crucial roles in response to biotic and abiotic stresses. However, little is known about the function of bZIP genes in soybean. In this study, we isolated a bZIP gene, GmbZIP19, from soybean. A subcellular localization study of GmbZIP19 revealed its nucleus localization. We showed that GmbZIP19 expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid), but reduced under salt and drought stress conditions. Further, GmbZIP19 overexpression Arabidopsis lines showed increased resistance to S. sclerotiorum and Pseudomonas syringae associated with upregulated ABA-, JA-, ETH- (ethephon-)and SA-induced marker genes expression, but exhibited sensitivity to salt and drought stresses in association with destroyed stomatal closure and downregulated the salt and drought stresses marker genes’ expression. We generated a soybean transient GmbZIP19 overexpression line, performed a Chromatin immunoprecipitation assay and found that GmbZIP19 bound to promoters of ABA-, JA-, ETH-, and SA-induced marker genes in soybean. The yeast one-hybrid verified the combination. The current study suggested that GmbZIP19 is a positive regulator of pathogen resistance and a negative regulator of salt and drought stress tolerance.

scholarly journals TheSsDREBTranscription Factor from the Succulent HalophyteSuaeda salsaEnhances Abiotic Stress Tolerance in Transgenic Tobacco

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Xu Zhang ◽  
Xiaoxue Liu ◽  
Lei Wu ◽  
Guihong Yu ◽  
Xiue Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Soluble Sugars ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Dreb Transcription Factor

Dehydration-responsive element-binding (DREB) transcription factor (TF) plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designatedSsDREB, was isolated from succulent halophyteSuaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed thatSsDREBwas significantly induced under salinity and drought stress. Overexpression ofSsDREBcDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that theSsDREBtranscription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression.

scholarly journals The bZIP Transcription Factor GmbZIP15 Negatively Regulates Salt- and Drought-Stress Responses in Soybean

2020 ◽  
Vol 21 (20) ◽  
pp. 7778
Author(s):  
Man Zhang ◽  
Yanhui Liu ◽  
Hanyang Cai ◽  
Mingliang Guo ◽  
Mengnan Chai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Negative Regulator ◽  
Bzip Transcription Factor ◽  
Antioxidant Enzyme Activities ◽  
Oilseed Crop ◽  
Abiotic Stress Response ◽  
Salt And Drought Stress

Soybean (Glycine max), as an important oilseed crop, is constantly threatened by abiotic stress, including that caused by salinity and drought. bZIP transcription factors (TFs) are one of the largest TF families and have been shown to be associated with various environmental-stress tolerances among species; however, their function in abiotic-stress response in soybean remains poorly understood. Here, we characterized the roles of soybean transcription factor GmbZIP15 in response to abiotic stresses. The transcript level of GmbZIP15 was suppressed under salt- and drought-stress conditions. Overexpression of GmbZIP15 in soybean resulted in hypersensitivity to abiotic stress compared with wild-type (WT) plants, which was associated with lower transcript levels of stress-responsive genes involved in both abscisic acid (ABA)-dependent and ABA-independent pathways, defective stomatal aperture regulation, and reduced antioxidant enzyme activities. Furthermore, plants expressing a functional repressor form of GmbZIP15 exhibited drought-stress resistance similar to WT. RNA-seq and qRT-PCR analyses revealed that GmbZIP15 positively regulates GmSAHH1 expression and negatively regulates GmWRKY12 and GmABF1 expression in response to abiotic stress. Overall, these data indicate that GmbZIP15 functions as a negative regulator in response to salt and drought stresses.

scholarly journals CaDHN5, a Dehydrin Gene from Pepper, Plays an Important Role in Salt and Osmotic Stress Responses

2019 ◽  
Vol 20 (8) ◽  
pp. 1989 ◽  
Author(s):  
Dan Luo ◽  
Xiaoming Hou ◽  
Yumeng Zhang ◽  
Yuancheng Meng ◽  
Huafeng Zhang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Manganese Superoxide Dismutase ◽  
Transgenic Arabidopsis ◽  
Abiotic Stress Tolerance ◽  
Late Embryogenesis Abundant ◽  
Virus Induced Gene Silencing ◽  
Dehydrin Gene ◽  
Salt And Osmotic Stress

Dehydrins (DHNs), as a sub-family of group two late embryogenesis-abundant (LEA) proteins, have attracted considerable interest owing to their functions in enhancing abiotic stress tolerance in plants. Our previous study showed that the expression of CaDHN5 (a dehydrin gene from pepper) is strongly induced by salt and osmotic stresses, but its function was not clear. To understand the function of CaDHN5 in the abiotic stress responses, we produced pepper (Capsicum annuum L.) plants, in which CaDHN5 expression was down-regulated using VIGS (Virus-induced Gene Silencing), and transgenic Arabidopsis plants overexpressing CaDHN5. We found that knock-down of CaDHN5 suppressed the expression of manganese superoxide dismutase (MnSOD) and peroxidase (POD) genes. These changes caused more reactive oxygen species accumulation in the VIGS lines than control pepper plants under stress conditions. CaDHN5-overexpressing plants exhibited enhanced tolerance to salt and osmotic stresses as compared to the wild type and also showed increased expression of salt and osmotic stress-related genes. Interestingly, our results showed that many salt-related genes were upregulated in our transgenic Arabidopsis lines under salt or osmotic stress. Taken together, our results suggest that CaDHN5 functions as a positive regulator in the salt and osmotic stress signaling pathways.

scholarly journals Molecular characterization of two AP2/ERF transcription factor genes from Egyptian tomato cultivar (Edkawy)

2017 ◽  
Vol 4 (1) ◽  
pp. 12 ◽  
Author(s):  
Kareem Mosa ◽  
Eslam Heb El-din ◽  
Ahmed Ismail ◽  
Fawzy El- Feky ◽  
Ali El-Refy
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Molecular Characterization ◽  
Abiotic Stress Tolerance ◽  
Isolation And Identification ◽  
Tomato Cultivar ◽  
Erf Transcription Factor

The tomato is ranked first amongst vegetable crops in Egypt in relation to surface area and production. The Egyptian tomato cultivar Edkawy has shown abiotic stress tolerance characteristics. However, there is not much information about the molecular characterization of this cultivar. Furthermore, information regarding the identification of abiotic stress tolerance genes from the Edkawy tomato cultivar is lacking. Here, we investigated the ability of the Edkawy cultivar to tolerate drought stress. Two varieties were used as a control in this study; Peto86 (sensitive variety) and Strain B (tolerant variety). Edkawy, Peto86 and Strain B varieties were exposed to drought stress by reducing the water supply gradually. Interestingly, Edkawy demonstrated a remarkable tolerance phenotype to drought stress. Furthermore, we identified and isolated two members of the AP2/ERF transcription factor family from Edkawy which are associated with abiotic stress, particularly drought, i.e. ERF1 and ERF5. Protein prediction, validation and active site prediction of ERF1 and ERF5 were also determined. In addition to the domain obtained by the pfam online tool, the interaction between Edkawy ERFs proteins and other proteins in the Solanaceae family was obtained. Furthermore, subcellular localization was determined by the ngLOC and Plant-mPLoc online tools. Characterization of the Edkawy tomato cultivar and isolation and identification of such transcription factors will help in the engineering of tomato plants with abiotic stress tolerance.

scholarly journals Cloning and Expression Analysis of bZIP Transcription Factor from Finger Millet (Eleusine coracana L.) Under Abiotic Stress Conditions

2022 ◽  
Vol 9 (1) ◽  
pp. 1-13
Author(s):  
Jenifer Lolita C
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Expression Analysis ◽  
Finger Millet ◽  
Abiotic Stress Tolerance ◽  
Eleusine Coracana ◽  
Stress Conditions ◽  
Cloning And Expression

Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic stress tolerance. In this study, we were attempted to study characterization of bZIP, a transcription factor from a climate smart cereal finger millet (Eleusine coracana L.). Seeds of Eleusine coracana (finger millet) was purchase from local market and were grown under field conditions drought and salt stress conditions. In this study, EcbZIP gene was isolated from finger millet, cloned into DH5α cells, screened by using colony PCR and expression analysis in response to two abiotic stresses was carried out by using qRT PCR. EcbZIP coding DNA sequence and protein sequence were retrieved from NCBI Nucleotide Database and Genpept of Accession number KP033192.1 and AJP67539.1 and validated by using SMART (simple modular architecture tool) Domain Tool. Cloning and expression studies were carried out using standardized molecular biology protocol. Results depicted that EcbZIP transcription factor showed significant upregulation under both salt and drought stress conditions, indicating that it plays an important role in tolerance towards these stresses. In conclusion, expression analysis of bZIP gene from finger millet seed cultivar ML-365 showed 5-fold upregulation to salt stress to drought stress and 8-fold upregulation to salt stress. Hence, it can serve as a candidate gene for improving abiotic stress tolerance and can be helpful in enhancing the crop productivity under stress conditions.

scholarly journals Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8716
Author(s):  
Lihua Xie ◽  
Miaomiao Cai ◽  
Xiangyu Li ◽  
Huifang Zheng ◽  
Yali Xie ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Leaf Senescence ◽  
Stress Responses ◽  
Abiotic Stress Tolerance ◽  
Moso Bamboo ◽  
Biotic And Abiotic Stress ◽  
Phyllostachys Edulis ◽  
Salt And Drought Stress ◽  
Nac Family

The NAC family is one of the largest transcription factor families unique to plants, which regulates the growth and development, biotic and abiotic stress responses, and maturation and senescence in plants. In this study, PheNAC3, a NAC gene, was isolated and characterized from moso bamboo (Phyllostachys edulis). PheNAC3 belong to the NAC1 subgroup and has a conserved NAC domain on the N-terminus, which with 88.74% similarity to ONAC011 protein. PheNAC3 localized in the nucleus and exhibited transactivation activity. PheNAC3 was upregulated during the process of senescence of leaves and detected shoots. PheNAC3 was also induced by ABA, MeJA, NaCl and darkness, but it had no remarkable response to PEG and SA treatments. Overexpression of PheNAC3 could cause precocious senescence in Arabidopsis. Transgenic Arabidopsis displayed faster seed germination, better seedling growth, and a higher survival rate than the wild-type under salt or drought stress conditions. Moreover, AtSAG12 associated with senescence and AtRD29A and AtRD29b related to ABA were upregulated by PheNAC3 overexpression, but AtCAB was inhibited. These findings show that PheNAC3 may participate in leaf senescence and play critical roles in the salt and drought stress response.

Heterologous Overexpression of ZmHDZIV13 Enhanced Drought and Salt Tolerance in Arabidopsis and Tobacco

2021 ◽  
Author(s):  
Fang Wang ◽  
Peng Fang ◽  
Huiping Yan ◽  
Xiangzhuo Ji ◽  
Yunling Peng
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Leucine Zipper ◽  
Abiotic Stress Tolerance ◽  
Heterologous Overexpression ◽  
Malondialdehyde Content ◽  
Drought And Salt Tolerance ◽  
Relative Water ◽  
Dependent Signaling Pathway

Abstract The homeodomain leucine zipper (HD-Zip) IV transcription factor is indispensable in the response of plants to abiotic stress. Systematic studies have been carried out in Arabidopsis, rice and other species from which a series of stress resistance-related genes have been isolated. However, the function of the HD-Zip-IV protein in maize is not clear. In this study, we cloned the HD-Zip-IV gene ZmHDZIV13 and identified its function in the stress response. Our phylogenetic analysis showed that ZmHDZIV13 and AtHDG11 had high homology and might have similar functions. The heterologous overexpression of ZmHDZIV13 in Arabidopsis resulted in sensitivity to abscisic acid (ABA), salt tolerance during germination and drought tolerance in seedlings. Under drought stress, the transgenic Arabidopsis showed stronger drought resistance than the wild-type showed (control). The malondialdehyde content of ZmHDZIV13 transgenic plants was lower than that of the control, and the relative water content and proline content were significantly higher than those of the control. After the drought was relieved, the expression of P5CS1, RD22, RD29B, RD29A, NCED3 and ERD1 were upregulated in transgenic Arabidopsis. Also, modified tobacco plants (35S::ZmHDZIV13) exhibited proper stomatal changes in response to drought conditions. These results show that ZmHDZIV13, as a stress-responsive transcription factor, plays a role in the positive regulation of abiotic stress tolerance and can regulate an ABA-dependent signaling pathway to regulate drought response in plants.

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