scholarly journals Morphological Identification of Lepidii Seu Descurainiae Semen and Adulterant Seeds Using Microscopic Analysis

2018 ◽  
Vol 8 (11) ◽  
pp. 2134 ◽  
Author(s):  
Jun-Ho Song ◽  
Byeong Moon ◽  
Goya Choi ◽  
Sungyu Yang
Keyword(s):  
Electron Microscope ◽  
Herbal Medicine ◽  
Northeast Asia ◽  
Microscopic Analysis ◽  
Morphological Identification ◽  
Traditional Herbal Medicine ◽  
Accurate Identification ◽  
Morphological Marker ◽  
Seed Identification ◽  
Scanning Electron

Lepidii seu Descurainiae Semen, the dried ripe seeds of Descurainia sophia (L.) Webb ex Prantl or Lepidium apetalum Willd., is used widely as a traditional herbal medicine in Northeast Asia. However, seeds of a number of other species have been misidentified as Lepidii seu Descurainiae Semen, and are therefore misused because of morphological similarities among the seeds of these species. To accurately identify Lepidii seu Descurainiae Semen, we investigated the morphology, mucilage reaction, and micromorphology of Lepidii seu Descurainiae Semen and the seeds of other plant species. We used a stereomicroscope and a scanning electron microscope to describe and compare various morphological and micromorphological characteristics of seeds, and a light microscope to determine the presence or absence of mucilage. We evaluated the potential usefulness of mucilage as a morphological marker for seed identification. The shape, outline, and size of seeds were useful characteristics for identification. The mucilage reaction and the three types of seed ornamentation that were identified in this study were particularly valuable for seed authentication. Based on these results, we created an identification key based on morphology and micromorphology of Lepidii seu Descurainiae Semen and adulterant seeds. The method of seed identification using microscopic examination, as described here, is easy and economical, making it a potential key tool for accurate identification of Lepidii seu Descurainiae Semen and seeds of other medicinal plants.

SEM analysis of dental enamel morphological structures on the basis of their replicas in patients with systemic calcium deficiency

2009 ◽  
Vol 4 (2) ◽  
pp. 190-195
Author(s):  
Ewa Bałczewska ◽  
Leszek Klimek ◽  
Aleksandra Palatyńska-Ulatowska
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Periodontal Diseases ◽  
Dental Enamel ◽  
Microscopic Analysis ◽  
Calcium Deficiency ◽  
Sem Analysis ◽  
Mineral Density ◽  
Dental Tissues ◽  
Scanning Electron

AbstractThe aim of the present study is to examine microscopically the surface of dental enamel by using a scanning electron microscope (SEM), using their replicas formed in female patients with diagnosed periodontal diseases and systemic calcium deficiency. Replicas of dental enamel surfaces in patients referred for treatment of periodontal diseases were subjected to microscopic analysis. The replicas, after coating with platinum-palladium alloy, were examined under the scanning electron microscope at magnifications of 15–5000 x. Densitometric examinations of spine (L2 - L4 segment) revealed bone mineral density BMD T-score lower than −2.5 in 5 patients, in the range of −1.5 to −2.5 in 10 patients, and higher than −1.5 in the remaining patients. Non-homogenous images of surfaces in the form of light and dark areas were observed. Light areas corresponded to damaged surfaces of dental tissues. Patients with higher systemic calcium deficiency had areas lighter in color. More of these areas were found in patients with higher systemic calcium deficiency. It can be assumed that the calcium deficit is likely to appear in the selected dental tissues, particularly in the dental enamel.

scholarly journals Morphological Identification of Bolbosoma turbinella (Acanthocephala) in Sei Whale (Balaenoptera borealis) from Straits of Malacca, Malaysia

2021 ◽  
Vol 13 (1) ◽  
pp. 83
Author(s):  
Mohd Ihwan Zakariah ◽  
Mohd Tamimi Ali Ahmad ◽  
Mohd Shafiq Razak ◽  
Norainy Mohd Husin ◽  
Wahidah Wahab ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Genomic Dna ◽  
Marine Mammals ◽  
East Coast ◽  
First Record ◽  
Peninsular Malaysia ◽  
Morphological Identification ◽  
Straits Of Malacca ◽  
Specific Species ◽  
Scanning Electron

HighlightIdentification of Morphological characteristic of the Bolbosoma sp.Genetic confirmation of the specific species of Bolbosoma turbinella.The first report in Straits of Malacca.Health status of Sei Whale (Balaenoptera borealis).AbstractThe study of ectoparasite and endoparasites of marine mammals are not habitually done because some species are endangered and protected by law. A stranded Sei Whale, Balaenoptera borealis (Anderson, 1878) from the East Coast of Peninsular Malaysia was examined for endoparasites. The objective of this study is to identify the species of acanthocephalan in the intestine of the Sei Whale found in the straits of Malacca. A total of ten parasite specimens were collected from the fresh intestine, and were fixed in 10% buffered formalin for further histological procedures. The morphological features of this parasite viewed under Scanning Electron Microscope (SEM) are referred to as the proboscis armature and variations in the spination of the area between the anterior and posterior cephalic bulb. Genomic DNA extracted by using QIAGEN DNeasy Blood & Tissue Kit DNA and sequenced with First Base Sequencer showed that this species belonged to Bolbosoma turbinella. This was the first record of a sei whale carrying the endoparasites Bolbosoma turbinella, in Malaysian waters.

scholarly journals Study of skin of an Egyptian mummy using a scanning electron microscope

2017 ◽  
Vol 80 (2) ◽  
pp. 233-242
Author(s):  
Hanna Mańkowska-Pliszka ◽  
Halina Przychodzeń ◽  
Michał Nawrot ◽  
Sylwia Tarka ◽  
Piotr Wasylczyk ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Microscopic Analysis ◽  
Unknown Origin ◽  
Ancient Egypt ◽  
Egyptian Mummy ◽  
Bacterial Endospores ◽  
The Right ◽  
First Time ◽  
Scanning Electron

AbstractThe first study of modified human remains using an electron microscope was carried out at the end of the 1950 and in 1979 the first result of the study involving a scanning electron microscope (SEM) was published for the first time. The study was mainly focused on the structure of tissues and cells. With the help of this technique cell and tissue elements, viruses and bacterial endospores as well as the structure of epithelium and the collagen contents of dermis were identified and described. In the above-mentioned case the object of the study using a SEM was a free part of the right hand (forearm with the dorsal and palmar parts of hand) of unknown origin, with signs of mummification revealed during microscopic analysis. Our study was aimed at finding the answer to the question if the mummification of the studied limb was natural or intentional, and if the study using a SEM could link the anonymous remains with ancient Egypt.

Somitogenesis in the amphibian Xenopus laevis: scanning electron microscopic analysis of intrasomitic cellular arrangements during somite rotation

Development ◽  
1981 ◽  
Vol 64 (1) ◽  
pp. 23-43
Author(s):  
B. Woo Youn ◽  
George M. Malacinski
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Xenopus Laevis ◽  
Microscopic Analysis ◽  
Block Rotation ◽  
Electron Microscopic ◽  
Cellular Changes ◽  
Scanning Electron Microscopic ◽  
Scanning Electron Microscopic Analysis ◽  
Scanning Electron

The intrasomitic changes in cell arrangement which accompany somite rotation during somitogenesis in Xenopus laevis were analysed with the scanning electron microscope (SEM). Longitudinal, horizontal fractures of whole embryos were examined at various dorsoventral levels of stage-22 to -24 embryos. Observations of the gross morphological features of somitogenesis, and the cellular changes which accompany somite segmentation and somite rotation were made. Several of these observations lead to modifications of previous models for the cellular basis of somitogenesis in Xenopus. Individual cellular rearrangements, rather than simultaneous block rotation of a whole somite, appear to be responsible for the 90° rotation of myotomal cells within a single somite. Cellular arrangments in fused somites were also examined. Some ultraviolet-irradiated embryos displayed a complete lack of a notochord. The somites in those embryos were fused across the midline beneath the neural tube. The dorsal and ventral arms of the somites are not fused. Normal rotation occurs only in the dorsal and ventral arms while, in the majority of cases, cells in the fused region fail to rotate normally. In some cases, individual cells in the fused region undergo partial rearrangement. Those observations support the notion that individual cellular rearrangements account for the rotation of the whole somite.

Forensic tools for the diagnosis of electrocution death: Case study and literature review

2018 ◽  
Vol 86 (2) ◽  
pp. 89-93 ◽  
Author(s):  
Cristina Mondello ◽  
Antonio Micali ◽  
Luigi Cardia ◽  
Antonina Argo ◽  
Stefania Zerbo ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Troponin I ◽  
Microscopic Analysis ◽  
Myocardial Damage ◽  
The Body ◽  
Variable Pressure ◽  
Creatine Kinase Mb ◽  
Iron And Zinc ◽  
Scanning Electron

Diagnosis of death by electrocution may be difficult when electric marking is not visible or unclear. Accordingly, the body of a man who appeared to have died from accidental electrocution was carefully forensically analysed. Macroscopic and microscopic analysis of the current mark was carried out using a variable-pressure scanning electron microscope equipped with energy dispersive X-ray microanalyser to highlight skin metallisation, indicating the presence of iron and zinc. The histological findings of electrocution myocardial damage were supported by the results of biochemical analysis which demonstrated the creatine kinase-MB and cardiac troponin I elevation. The effects of electric current flow were also highlighted by perforations of endothelial surface of a pulmonary artery using scanning electron microscope, and all the results were analysed by the main tools suggested in the literature.

Morphology, Distribution and Identification of Micro-Constituents Along Grain Boundaries in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 176-177
Author(s):  
D. E. Fornwalt ◽  
A. R. Geary ◽  
B. H. Kear
Keyword(s):  
Electron Microscope ◽  
Grain Boundaries ◽  
Volume Fraction ◽  
Rupture Life ◽  
Stress Rupture ◽  
High Volume ◽  
Precipitate Morphology ◽  
Stress Rupture Life ◽  
Nickel Base ◽  
Scanning Electron

A systematic study has been made of the effects of various heat treatments on the microstructures of several experimental high volume fraction γ’ precipitation hardened nickel-base alloys, after doping with ∼2 w/o Hf so as to improve the stress rupture life and ductility. The most significant microstructural chan§e brought about by prolonged aging at temperatures in the range 1600°-1900°F was the decoration of grain boundaries with precipitate particles.Precipitation along the grain boundaries was first detected by optical microscopy, but it was necessary to use the scanning electron microscope to reveal the details of the precipitate morphology. Figure 1(a) shows the grain boundary precipitates in relief, after partial dissolution of the surrounding γ + γ’ matrix.

Preparatory Procedures for Electron Microscopic Analysis at the Molecular Level of Resolution

1978 ◽  
Vol 36 (3) ◽  
pp. 516-520
Author(s):  
F.J. Sjostrand
Keyword(s):  
Electron Microscope ◽  
Molecular Level ◽  
Microscopic Analysis ◽  
Resolving Power ◽  
Cellular Structures ◽  
Electron Microscopic ◽  
Systematic Analysis ◽  
Technical Developments ◽  
Thin Sectioning ◽  
Obvious Reason

In the 1940's and 1950's electron microscopy conferences were attended with everybody interested in learning about the latest technical developments for one very obvious reason. There was the electron microscope with its outstanding performance but nobody could make very much use of it because we were lacking proper techniques to prepare biological specimens. The development of the thin sectioning technique with its perfectioning in 1952 changed the situation and systematic analysis of the structure of cells could now be pursued. Since then electron microscopists have in general become satisfied with the level of resolution at which cellular structures can be analyzed when applying this technique. There has been little interest in trying to push the limit of resolution closer to that determined by the resolving power of the electron microscope.

The Alteration of the Pore Spaces in Sandstones

1973 ◽  
Vol 31 ◽  
pp. 210-211
Author(s):  
R. E. Ferrell ◽  
G. G. Paulson
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Other ◽  
Coarse Grained ◽  
Depositional Fabric ◽  
Soluble Components ◽  
Scanning Electron ◽  
Shape And Size

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

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