PRGF-Modified Collagen Membranes for Guided Bone Regeneration: Spectroscopic, Microscopic and Nano-Mechanical Investigations

The aim of our study was to evaluate the properties of different commercially available resorbable collagen membranes for guided bone regeneration, upon addition of plasma rich in growth factors (PRGF). The structural and morphological details, mechanical properties, and enzymatic degradation were investigated in a new approach, providing clinicians with new data in order to help them in a successful comparison and better selection of membranes with respect to their placement and working condition. Particular characteristics such as porosity, fiber density, and surface topography may influence the mechanical behavior and performances of the membranes, as revealed by SEM/AFM and nanoindentation measurements. The mechanical properties and enzymatic degradation of the membranes were analyzed in a comparative manner, before and after PRGF-modification. The changes in Young modulus values are correlated with the ultrastructural properties of each membrane type. The enzymatic (trypsin) degradation test also emphasized that PRGF-modified membranes exhibit a slower degradation compared to the native ones.

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Evaluation of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Cross-Linked Collagen Membranes for Guided Bone Regeneration in Beagle Dogs

Materials ◽

10.3390/ma13204599 ◽

2020 ◽

Vol 13 (20) ◽

pp. 4599

Author(s):

Jong-Ju Ahn ◽

Hyung-Joon Kim ◽

Eun-Bin Bae ◽

Won-Tak Cho ◽

YunJeong Choi ◽

...

Keyword(s):

Tensile Strength ◽

Physical Properties ◽

Bone Regeneration ◽

Enzymatic Degradation ◽

Test Group ◽

Guided Bone Regeneration ◽

Control Group ◽

Collagen Membrane ◽

Micro Computed Tomography ◽

Collagen Membranes

The purpose of this study was to evaluate the bone regeneration efficacy of an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked collagen membrane for guided bone regeneration (GBR). A non-cross-linked collagen membrane (Control group), and an EDC-cross-linked collagen membrane (Test group) were used in this study. In vitro, mechanical, and degradation testing and cell studies were performed. In the animal study, 36 artificial bone defects were formed in the mandibles of six beagles. Implants were inserted at the time of bone grafting, and membranes were assigned randomly. Eight weeks later, animals were sacrificed, micro-computed tomography was performed, and hematoxylin-eosin stained specimens were prepared. Physical properties (tensile strength and enzymatic degradation rate) were better in the Test group than in the Control group. No inflammation or membrane collapse was observed in either group, and bone volumes (%) in defects around implants were similar in the two groups (p > 0.05). The results of new bone areas (%) analysis also showed similar values in the two groups (p > 0.05). Therefore, it can be concluded that cross-linking the collagen membranes with EDC is the method of enhancing the physical properties (tensile strength and enzymatic degradation) of the collagen membranes without risk of toxicity.

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Zn-Containing Membranes for Guided Bone Regeneration in Dentistry

Polymers ◽

10.3390/polym13111797 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1797

Author(s):

Manuel Toledano ◽

Marta Vallecillo-Rivas ◽

María T. Osorio ◽

Esther Muñoz-Soto ◽

Manuel Toledano-Osorio ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Regeneration ◽

Bone Healing ◽

Bone Repair ◽

Complex Modulus ◽

Bacterial Colonization ◽

Guided Bone Regeneration ◽

M2 Macrophage

Barrier membranes are employed in guided bone regeneration (GBR) to facilitate bone in-growth. A bioactive and biomimetic Zn-doped membrane with the ability to participate in bone healing and regeneration is necessary. The aim of the present study is to state the effect of doping the membranes for GBR with zinc compounds in the improvement of bone regeneration. A literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken, focusing on the antibacterial effects, physicochemical and biological properties of Zn-loaded membranes. Bioactivity, bone formation and cytotoxicity were analyzed. Microstructure and mechanical properties of these membranes were also determined. Zn-doped membranes have inhibited in vivo and in vitro bacterial colonization. Zn-alloy and Zn-doped membranes attained good biocompatibility and were found to be non-toxic to cells. The Zn-doped matrices showed feasible mechanical properties, such as flexibility, strength, complex modulus and tan delta. Zn incorporation in polymeric membranes provided the highest regenerative efficiency for bone healing in experimental animals, potentiating osteogenesis, angiogenesis, biological activity and a balanced remodeling. Zn-loaded membranes doped with SiO2 nanoparticles have performed as bioactive modulators provoking an M2 macrophage increase and are a potential biomaterial for promoting bone repair. Zn-doped membranes have promoted pro-healing phenotypes.

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Selection of Collagen Membranes for Bone Regeneration: A Literature Review

Materials ◽

10.3390/ma13030786 ◽

2020 ◽

Vol 13 (3) ◽

pp. 786 ◽

Cited By ~ 7

Author(s):

Luca Sbricoli ◽

Riccardo Guazzo ◽

Marco Annunziata ◽

Luca Gobbato ◽

Eriberto Bressan ◽

...

Keyword(s):

Bone Regeneration ◽

Clinical Performance ◽

Guided Bone Regeneration ◽

Treatment Modalities ◽

Barrier Membranes ◽

Collagen Membranes ◽

Collagen Versus ◽

The Right ◽

Grafting Materials ◽

Selection Of

Several treatment modalities have been proposed to regenerate bone, including guided bone regeneration (GBR) where barrier membranes play an important role by isolating soft tissue and allowing bone to grow. Not all membranes biologically behave the same way, as they differ from their origin and structure, with reflections on their mechanical properties and on their clinical performance. Collagen membranes have been widely used in medicine and dentistry, because of their high biocompatibility and capability of promoting wound healing. Recently, collagen membranes have been applied in guided bone regeneration with comparable outcomes to non-resorbable membranes. Aim of this work is to provide a review on the main features, application, outcomes, and clinical employment of the different types of collagen membranes. Comparisons with non-resorbable membranes are clarified, characteristics of cross-linked collagen versus native collagen, use of different grafting materials and need for membrane fixation are explored in order to gain awareness of the indications and limits and to be able to choose the right membrane required by the clinical condition.

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Effect of Two Different Bioabsorbable Collagen Membranes on Guided Bone Regeneration: A Comparative Histomorphometric Study in the Dog Mandible

Journal of Periodontology ◽

10.1902/jop.2007.070102 ◽

2007 ◽

Vol 78 (10) ◽

pp. 1943-1953 ◽

Cited By ~ 49

Author(s):

Michael M. Bornstein ◽

Dieter Bosshardt ◽

Daniel Buser

Keyword(s):

Bone Regeneration ◽

Guided Bone Regeneration ◽

Histomorphometric Study ◽

Collagen Membranes

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One-stage horizontal guided bone regeneration with autologous bone, anorganic bovine bone and collagen membranes - 3-year follow-up from a prospective study

Clinical Oral Implants Research ◽

10.1111/clr.291_13358 ◽

2018 ◽

Vol 29 ◽

pp. 406-406

Author(s):

Silvio Mario Meloni ◽

Edoardo Baldoni ◽

Milena Pisano ◽

Marco Duvina

Keyword(s):

Prospective Study ◽

Bone Regeneration ◽

Guided Bone Regeneration ◽

Bovine Bone ◽

One Stage ◽

Autologous Bone ◽

Collagen Membranes ◽

A Prospective Study

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Bioresorbable Collagen Membranes for Guided Bone Regeneration

Bone Regeneration ◽

10.5772/34667 ◽

2012 ◽

Cited By ~ 4

Author(s):

Haim Tal ◽

Ofer Moses ◽

Avital Kozlovsky ◽

Carlos Nemcovsky

Keyword(s):

Bone Regeneration ◽

Guided Bone Regeneration ◽

Collagen Membranes

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Improvement of the Physical Properties of Guided Bone Regeneration Membrane from Porcine Pericardium by Polyphenols-Rich Pomace Extract

Materials ◽

10.3390/ma12162564 ◽

2019 ◽

Vol 12 (16) ◽

pp. 2564 ◽

Cited By ~ 5

Author(s):

Nazario Russo ◽

Clara Cassinelli ◽

Elisa Torre ◽

Marco Morra ◽

Giorgio Iviglia

Keyword(s):

Physical Properties ◽

Bone Regeneration ◽

Enzymatic Degradation ◽

Guided Bone Regeneration ◽

Mechanical Stiffness ◽

Infra Red ◽

Space Maintenance ◽

The One ◽

Porcine Pericardium

To achieve optimal performances, guided bone regeneration membranes should have several properties, in particular, proper stiffness and tear resistance for space maintenance, appropriate resorption time, and non-cytotoxic effect. In this work, polyphenol-rich pomace extract (PRPE), from a selected grape variety (Nebbiolo), rich in proanthocyanidins and flavonols (e.g., quercetin), was used as a rich source of polyphenols, natural collagen crosslinkers, to improve the physical properties of the porcine pericardium membrane. The incorporation of polyphenols in the collagen network of the membrane was clearly identified by infra-red spectroscopy through the presence of a specific peak between 1360–1380 cm−1. Polyphenols incorporated into the pericardium membrane bind to collagen with high affinity and reduce enzymatic degradation by 20% compared to the native pericardium. The release study shows a release of active molecules from the membrane, suggesting a possible use in patients affected by periodontitis, considering the role of polyphenols in the control of this pathology. Mechanical stiffness is increased making the membrane easier to handle. Young’s modulus of pericardium treated with PRPE was three-fold higher than the one measured on native pericardium. Tear and suture retention strength measurement suggest favorable properties in the light of clinical practice requirements.

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Evaluation of Bone Gain and Complication Rates after Guided Bone Regeneration with Titanium Foils: A Systematic Review

Materials ◽

10.3390/ma13235346 ◽

2020 ◽

Vol 13 (23) ◽

pp. 5346

Author(s):

Elisabet Roca-Millan ◽

Enric Jané-Salas ◽

Albert Estrugo-Devesa ◽

José López-López

Keyword(s):

Systematic Review ◽

Clinical Trials ◽

Bone Regeneration ◽

Randomized Clinical Trials ◽

Guided Bone Regeneration ◽

Cochrane Library ◽

Complication Rates ◽

Native Bone ◽

Collagen Membranes ◽

Bone Gain

Guided bone regeneration techniques are increasingly used to enable the subsequent placement of dental implants. This systematic review aims to analyze the success rate of these techniques in terms of bone gain and complications rate using titanium membranes as a barrier element. Electronic and hand searches were conducted in PubMed/Medline, Scielo, Scopus and Cochrane Library databases for case reports, case series, cohort studies and clinical trials in humans published up to and including 19 September 2020. Thirteen articles were included in the qualitative analysis. Bone gain both horizontally and vertically was comparable to that obtained with other types of membranes more commonly used. The postoperative complication rate was higher that of native collagen membranes and non-resorbable titanium-reinforced membranes, and similar that of crosslinked collagen membranes and titanium meshes. The survival rate of the implants was similar to that of implants placed in native bone. Due to the limited scientific literature published on this issue, more randomized clinical trials comparing occlusive titanium barriers and other types of membranes are necessary to reach more valid conclusions.

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