scholarly journals Nanotechnological advances of Lipid film based biosensors for the rapid detection of biological compounds and toxicants

2020 ◽  
pp. 27-35
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Keyword(s):  
Lipid Membranes ◽  
Rapid Detection ◽  
Lipid Membrane ◽  
Cholesterol Oxidase ◽  
Lipid Film ◽  
Biological Interest ◽  
Wide Range ◽  
Biological Compounds ◽  
Novel Devices ◽  
D Dimer

The exploration of lipid membranes for the construction of nanobiosensors has recently provided the opportunity to construct devices to monitor a wide range of compounds of biological interest. Nanobiosensor miniaturization using nanotechnological tools has given novel ways to attach a wide range of “receptors” in the lipid membrane. The lipids used to construct a lipid film based device are dipalmiloylphosphatidylcholine {DPPC} and in some cases dipalmitoylphosphatidic acid (DPPA) which is an anionic lipid and is used to increase the sensitivity of detection. Most common “receptors” used in lipid film biosensors are enzymes such as urease, cholesterol oxidase, urecase, etc, antibodies such as D-dimer antibody and artificial or natural receptors such as saxitoxin, cholera toxin, calyx[4]arene phospjoryl receptor, etc. This chapter reviews and investigates the construction of nanobiosensors based on lipid membranes that are used to monitor various toxicants. It also exploits examples of applications with an emphasis on novel devices, new nanobiosensing techniques and nanotechnology-based transduction schemes. The compounds that can be detected are insecticides, toxins, hormones, dioxins, etc.

scholarly journals Nanotechnological advances of Lipid film-based biosensors for the rapid detection of biological compounds and toxicants

2019 ◽  
pp. 32-40
Author(s):  
Georgia-Paraskevi Nikoleli
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Cholesterol Oxidase ◽  
Environmental Pollutants ◽  
Clinical Analysis ◽  
Lipid Film ◽  
Biological Interest ◽  
Wide Range ◽  
Biological Compounds ◽  
Novel Devices

The exploration of lipid membranes for the construction of nanobiosensors has recently provided the opportunity to construct devices to monitor a wide range of compounds of biological interest. Nanobiosensor miniaturization using nanotechnological tools has given novel ways to attach a wide range of “receptors” in the lipid membrane. The lipids used to construct a lipid film-based device are dipalmiloylphosphatidylcholine {DPPC} and in some cases dipalmitoylphosphatidic acid (DPPA) which is an anionic lipid and is used to increase the sensitivity of detection. Most common “receptors” used in lipid film biosensors are enzymes such as urease, cholesterol oxidase, urecase, etc, antibodies such as D-dimer antibody and artificial or natural receptors such as saxitoxin, cholera toxin, calyx [4] arene phospjoryl receptor, etc. This chapter reviews and investigates the construction of nanobiosensors based on lipid membranes that are used to monitor various toxicants. It also exploits examples of applications with an emphasis on novel devices, new nanobiosensing techniques and nanotechnology-based transduction schemes. The compounds that can be detected are insecticides, toxins, hormones, dioxins, etc. Keywords: Lipid membrane based nanosensors; Nanoyechology; Graphene and ZnO electrodes; Food toxicants; Environmental pollutants; Clinical analysis

scholarly journals Ανάπτυξη και αξιολόγηση μεθόδων προσδιορισμού PCBs στα τρόφιμα

2012 ◽  
Author(s):  
Αντώνιος Μιχαλολιάκος
Keyword(s):  
Electrolyte Solution ◽  
Rapid Detection ◽  
Active Sites ◽  
Lipid Membrane ◽  
Flow Mode ◽  
Raman Spectrometry ◽  
Lipid Film ◽  
Real Samples ◽  
Wide Range ◽  
Aroclor 1242

Polychlorinated biphenyls (PCBs) are industrial compounds which are known to be among the most persistent and widely distributed pollutants in the global ecosystem. Since they are lipophilic, they tend to bioaccumulate in the fatty tissues of living organisms, including humans. Owing to the chemical and physical properties of PCBs, the analysis by conventional methods is difficult and expensive. Therefore simple methods for the detection of PCBs in environmental, industrial and food samples are required. The Ph.D. thesis involved the study, design and construction of electrochemical bilayer lipid membrane-based biosensors for rapid detection of compounds of biomedical, environmental and industrial interest such as PCBs. This work describes a novel electrochemical biosensor based on a supported polymerized lipid film with incorporated Sheep anti-PCB antibody for the rapid detection of aroclor 1242, at the levels of 10-9 M concentrations, in flowing solution streams. The antibody was incorporated into the lipid film during polymerization. Injections of Aroclor 1242 antigen solutions were made into flowing streams of a carrier electrolyte solution. Experiments were done in a stopped-flow mode using lipid mixtures containing 15% (w/w) dipalmitoylphosphatidic acid (DPPA) to provide only a single transient current signal with a magnitude related to the antigen concentration. An immunosensor based on the BLM transduction scheme should be regenerable and capable of multiple analyses. Thus, lipid films containing 35% DPPA were used to examine regeneration of the active sites of antibody after complex formation by washing with the carrier electrolyte solution. Repetitive cycles of injection of antigen have shown that the maximum number of cycles is about 5. The mechanism of signal generation was investigated by physicochemical methods of IR, Raman spectrometry and Scanning Electron Microscopy. The device was tested/ evaluated in real samples of vegetables. The investigation of the effect of potent interferences included a wide range of compounds usually found in foods. The results showed no interferences from these compounds in concentration levels usually found in real samples. The analyses that have been concluded in order to define the PCBs in the foods as well as the comparison of these results against the results of other established methods have proved that the biosensor used provides reliable results and it can therefore constitute a valuable tool for future applications in the field of Environmental Chemistry.

scholarly journals The Application of Lipid Membranes in Biosensing

Membranes ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 108 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Dimitrios Nikolelis ◽  
Christina Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Food Quality ◽  
Lipid Membranes ◽  
Recent Progress ◽  
Lipid Membrane ◽  
Environmental Pollutants ◽  
Quality Monitoring ◽  
Future Prospects ◽  
Considerable Part ◽  
Lipid Film ◽  
Food Toxicants

The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. This paper reviews recent progress in biosensor technologies based on lipid membranes suitable for food quality monitoring and environmental applications. Numerous biosensing applications based on lipid membrane biosensors are presented, putting emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The range of analytes that can be currently using these lipid film devices that can be detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/validation and eventually commercialization of the proposed lipid membrane-based biosensors.

scholarly journals Potentiometric cholesterol biosensing application of graphene electrode with stabilized polymeric lipid membrane

2013 ◽  
Vol 11 (9) ◽  
pp. 1554-1561 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Zafar Ibupoto ◽  
Dimitrios Nikolelis ◽  
Vlassis Likodimos ◽  
Nikolas Psaroudakis ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Cholesterol Oxidase ◽  
Biological Applications ◽  
Blood Samples ◽  
Graphene Electrode ◽  
Oxidase Enzyme ◽  
Polymerization Mixture ◽  
Linear Slope ◽  
Cholesterol Biosensor

AbstractAbstract A novel potentiometric cholesterol biosensor has been fabricated through the immobilization of the stabilized polymeric lipid membrane onto graphene electrode. The stabilized polymeric lipid membrane is composed of cholesterol oxidase enzyme and polymerization mixture; which holds paramount influence on the properties of the cholesterol biosensor. The presented biosensor reveals an appreciable reproducibility, good selectivity and high sensing capability with a linear slope curve of ∼64 mV per decade. The strong biocompatibility among stabilized polymeric lipid membranes and human biofluids provides the possibility to use for real blood samples and other biological applications. Graphical abstract

scholarly journals Potentiometric Biosensing Applications of Graphene Electrode with Stabilized Polymer Lipid Membranes

2018 ◽  
Author(s):  
Georgia-Pareaskevi Nikoleli ◽  
Dimitrios P. Nikolelis ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Stephanos Karapetis
Keyword(s):  
Lipid Membranes ◽  
Rapid Detection ◽  
Toxic Substance ◽  
Environmental Pollutants ◽  
High Sensitivity ◽  
Fast Response ◽  
Toxic Compounds ◽  
Wide Range ◽  
Sensitivity And Selectivity ◽  
Clinical Interest

This review provides informations and details for the fabrication of biosensors that are composed from lipid membranes and have been utilized and applied to rapidly detect food toxic compounds, environmental pollutants and analytes of clinical interest. Biosensors based on polymeric lipid membranes have been used to rapidly detect a wide range of these analytes and offer several advantages such as fast response, high sensitivity and selectivity, can be portable for in the field applications, and small size. A description of the construction of these devices and their applications for the rapid detection of food toxic substance, environmental pollutants and analytes of clinical interest is provided in this review.

scholarly journals Recent Lipid Membrane-Based Biosensing Platforms

2019 ◽  
Vol 9 (9) ◽  
pp. 1745 ◽  
Author(s):  
Georgia-Paraskevi Nikoleli ◽  
Christina G. Siontorou ◽  
Marianna-Thalia Nikolelis ◽  
Spyridoula Bratakou ◽  
Dimitrios K. Bendos
Keyword(s):  
Lipid Membrane ◽  
Clinical Chemistry ◽  
Environmental Pollutants ◽  
Membrane Technology ◽  
Lipid Film ◽  
Wide Range ◽  
Lipid Films ◽  
Clinical Interest ◽  
Food Toxicants

The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.

scholarly journals Assessing the use of ellipsoidal microparticles for determining lipid membrane viscosity

2021 ◽  
Author(s):  
Philip E. Jahl ◽  
Raghuveer Parthasarathy
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Translational Diffusion ◽  
Lipid Domains ◽  
Giant Vesicles ◽  
Membrane Viscosity ◽  
Cellular Processes ◽  
Wide Range ◽  
Chain Lengths ◽  
Good Agreement

The viscosity of lipid membranes sets the timescales of membrane-associated flows and therefore influences the dynamics of a wide range of cellular processes. Techniques to measure membrane viscosity remain sparse, however, and reported measurements to date, even of similar systems, give viscosity values that span orders of magnitude. To address this, we improve a method based on measuring both the rotational and translational diffusion of membrane-anchored microparticles and apply this approach and one based on tracking the motion of phase-separated lipid domains to the same system of phase-separated giant vesicles. We find good agreement between the two methods, with inferred viscosities within a factor of two of each other. Our technique uses ellipsoidal microparticles, and we show that the extraction of physically meaningful viscosity values from their motion requires consideration of their anisotropic shape. The validation of our method on phase-separated membranes makes possible its application to other systems, which we demonstrate by measuring the viscosity of bilayers composed of lipids with different chain lengths ranging from 14 to 20 carbon atoms, revealing a very weak dependence of two-dimensional viscosity on lipid size. The experimental and analysis methods described here should be generally applicable to a variety of membrane systems, both reconstituted and cellular.

scholarly journals Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek
Keyword(s):  
Electrochemical Properties ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Membrane Resistance ◽  
Electrochemical Characteristics ◽  
Freeze Thaw ◽  
Lipid Films ◽  
Supported Lipid Membranes

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.

scholarly journals Interactions of Linear Analogues of Battacin with Negatively Charged Lipid Membranes

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 192
Author(s):  
Kinga Burdach ◽  
Dagmara Tymecka ◽  
Aneta Urban ◽  
Robert Lasek ◽  
Dariusz Bartosik ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Liquid Crystalline ◽  
Lipid Membrane ◽  
Attenuated Total Reflection ◽  
Gram Positive ◽  
Insertion Depth ◽  
Force Microscopy ◽  
Acyl Chains ◽  
Hydrophobic Portion ◽  
Membrane Fluidization

The increasing resistance of bacteria to available antibiotics has stimulated the search for new antimicrobial compounds with less specific mechanisms of action. These include the ability to disrupt the structure of the cell membrane, which in turn leads to its damage. In this context, amphiphilic lipopeptides belong to the class of the compounds which may fulfill this requirement. In this paper, we describe two linear analogues of battacin with modified acyl chains to tune the balance between the hydrophilic and hydrophobic portion of lipopeptides. We demonstrate that both compounds display antimicrobial activity with the lowest values of minimum inhibitory concentrations found for Gram-positive pathogens. Therefore, their mechanism of action was evaluated on a molecular level using model lipid films mimicking the membrane of Gram-positive bacteria. The surface pressure measurements revealed that both lipopeptides show ability to bind and incorporate into the lipid monolayers, resulting in decreased ordering of lipids and membrane fluidization. Atomic force microscopy (AFM) imaging demonstrated that the exposure of the model bilayers to lipopeptides leads to a transition from the ordered gel phase to disordered liquid crystalline phase. This observation was confirmed by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) results, which revealed that lipopeptide action causes a substantial increase in the average tilt angle of lipid acyl chains with respect to the surface normal to compensate for lipopeptide insertion into the membrane. Moreover, the peptide moieties in both molecules do not adopt any well-defined secondary structure upon binding with the lipid membrane. It was also observed that a small difference in the structure of a lipophilic chain, altering the balance between hydrophobic and hydrophilic portion of the molecules, results in different insertion depth of the active compounds.

Optical portable biosensors based on stabilized lipid membrane for the rapid detection of doping materials in human urine

2008 ◽  
Vol 130 (2) ◽  
pp. 577-582 ◽  
Author(s):  
Dimitrios P. Nikolelis ◽  
Garyfallia Raftopoulou ◽  
Petros Chatzigeorgiou ◽  
Georgia-Paraskevi Nikoleli ◽  
Kyriakos Viras
Keyword(s):  
Human Urine ◽  
Rapid Detection ◽  
Lipid Membrane
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