scholarly journals Release of VEGF from Dental Implant Surface (IML® Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations

2019 ◽  
Vol 9 (10) ◽  
pp. 2114 ◽  
Author(s):  
Andrea Palermo ◽  
Franco Ferrante ◽  
Eleonora Stanca ◽  
Fabrizio Damiano ◽  
Antonio Gnoni ◽  
...  
Keyword(s):  
Growth Factors ◽  
Liquid Phase ◽  
Growth Factor ◽  
Dental Implant ◽  
Biologically Active ◽  
Future Expectations ◽  
Implant Surface ◽  
Dental Implant Surface ◽  
Concentrated Growth Factors

This study aimed to evaluate the combined use of the Concentrated Growth Factor (CGF) and the liquid phase of CGF (LPCGF) on dental implant surfaces, using a medical device to determine the migration of growth factors, from the implant surface to the recipient. The implants were permeated by autologous growth factors, using a specific centrifuge device. CGF adhesion on the implant surface was evaluated through a scanning electron microscope analysis. To assess the release of the vascular endothelial growth factor (VEGF) from CGF, LPCGF, and CGF- or LPCGF-permeated implant, an ELISA assay was carried out. The results showed that the concentration of the growth factor VEGF was greater in CGF than in LPCGF. Our innovative technique allowed the incorporation of autologous growth factors on the surface of the dental implants. Moreover, we reported the release of VEGF, over time, by CGF- or LPCGF-permeated implant. On this basis, it was possible to obtain a biologically active implant surface, essential to create intercellular communication and neo-angiogenesis, to facilitate wound healing and tissue regeneration.

scholarly journals Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Carlos Nelson Elias ◽  
Patricia Abdo Gravina ◽  
Costa e Silva Filho ◽  
Pedro Augusto de Paula Nascente
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Osteoblastic Cells ◽  
Acid Etching ◽  
Implant Surface ◽  
Force Microscopy ◽  
Dental Implant Surface ◽  
Before And After ◽  
Titanium Surfaces

Statement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength.Objective. In this work, the effects of dental implant surface treatment and fibronectin adsorption on the adhesion of osteoblasts were analyzed.Materials and Methods. Two titanium dental implants (Porous-acid etching and PorousNano-acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy, and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine their effects on the interaction with osteoblastic cells.Results. The evaluation techniques used showed that the Porous and PorousNano implants have similar microstructural characteristics. Spectrophotometry demonstrated similar levels of fibronectin adsorption on both surfaces (80%). The association indexes of osteoblastic cells in FN-treated samples were significantly higher than those in samples without FN. The radioactivity values associated with the same samples, expressed as counts per minute (cpm), suggested that FN incorporation is an important determinant of thein vitrocytocompatibility of the surfaces.Conclusion. The preparation of bioactive titanium surfaces via fluoride and FN retention proved to be a useful treatment to optimize and to accelerate the osseointegration process for dental implants.

scholarly journals Porphyromonas Gingivalis Biofilm Formation on an Implant Surface Treated With Nanoselenium

2020 ◽  
Author(s):  
Mohamed Assadawy ◽  
Eman Helmy
Keyword(s):  
Dental Implant ◽  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Practical Implication ◽  
Antibacterial Properties ◽  
Implant Surface ◽  
Sem Images ◽  
Dental Implant Surface ◽  
Implant Coatings

Abstract Background: Biofilm formation on implants is the primary factor for implant loss. Porphyromonas gingivalis is a highly virulent pathogen that contributes to the development of periodontal disease and implant failure.Objectives: The goals of this study are to investigate the formation of P. gingivalis biofilms on nanoselenium-coated implants in vitro and the potential use of nanoselenium for peri-implantitis treatment.Materials and methods: Porphyromonas gingivalis ATCC 33277 was cultured to obtain an in vitro mature biofilm on the surface of the Hexacone implant system. The fixture was added into an Eppendorf tube and placed in a sterile air laminar flow cabinet. An automatic machine learning utility was used to calculate the biofilm size on the implant surface from SEM images, and the Trainable Weka Segmentation plugin in Fiji software was employed.Results The SeNPs affected the P. gingivalis biofilm (the effect size was 80.17%), and the difference was highly significant (p 0.000).Conclusion: The use of SeNPs as dental implant coatings presented promising anti-P. gingivalis biofilm activity.Clinical relevance:: The development of a dental implant surface treatment with efficient antibacterial properties, especially against the most virulent pathogens, has not yet been established.Principal findings: Nanoselenium particles as an implant surface coating prevented Porphyromonas gingivalis biofilm formation to a striking extent.Practical implication: Nanoparticles could provide a novel state-of-the-art therapeutic approach for Porphyromonas gingivalis (P. gingivalis biofilm on dental implants)

The use of laser therapy for dental implant surface decontamination: a narrative review of in vitro studies

2013 ◽  
Vol 29 (6) ◽  
pp. 1977-1985 ◽  
Author(s):  
Marina Salah Kamel ◽  
Amardeep Khosa ◽  
Andrew Tawse-Smith ◽  
Jonathan Leichter
Keyword(s):  
Laser Therapy ◽  
Dental Implant ◽  
Narrative Review ◽  
In Vitro Studies ◽  
Implant Surface ◽  
Dental Implant Surface ◽  
Surface Decontamination

scholarly journals In vitro comparison of two titanium dental implant surface treatments: 3M™ESPE™ MDIs versus Ankylos®

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Jagjit Singh Dhaliwal ◽  
Juliana Marulanda ◽  
Jingjing Li ◽  
Sharifa Alebrahim ◽  
Jocelyne Sheila Feine ◽  
...  
Keyword(s):  
Dental Implant ◽  
Surface Treatments ◽  
Implant Surface ◽  
Dental Implant Surface ◽  
Titanium Dental Implant ◽  
In Vitro Comparison

scholarly journals Dental Pulp Stem Cells on Implant Surface: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Luigi Laino ◽  
Marcella La Noce ◽  
Luca Fiorillo ◽  
Gabriele Cervino ◽  
Ludovica Nucci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Implant ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Implant Surface ◽  
Oral Surgical Procedures ◽  
Excellent Starting Point ◽  
Starting Point ◽  
Dental Implant Surface

In the field of biology and medicine, one hears often about stem cells and their potential. The dental implant new surfaces, subjected to specific treatments, perform better and allow for quicker healing times and better clinical performance. The purpose of this study is to evaluate from a biological point of view the interaction and cytotoxicity between stem cells derived from dental pulp (DPSCs) and titanium surfaces. Through the creation of complex cells/implant, this study is aimed at analyzing the cytotoxicity of dental implant surfaces (Myth (Maipek Manufacturer Industrial Care, Naples, Italy)) and the adhesion capacity of cells on them and at considering the essential factors for implant healing such as osteoinduction and vasculogenesis. These parameters are pointed out through histology (3D cell culture), immunofluorescence, proliferation assays, scanning electron microscopy, and PCR investigations. The results of the dental implant surface and its interaction with the DPSCs are encouraging, obtaining results increasing the mineralization of the tissues. The knowledge of this type of interaction, highlighting its chemical and biological features, is certainly also an excellent starting point for the development of even more performing surfaces for having better healing in the oral surgical procedures related to dental implant positioning.

scholarly journals Effect of Magnesium-Based Coatings on Titanium or Zirconia Substrates on Bone Regeneration and Implant Osseointegration- A Systematic Review

2021 ◽  
Vol 8 ◽  
Author(s):  
Ahmad H Almehmadi
Keyword(s):  
Systematic Review ◽  
Dental Implant ◽  
Animal Studies ◽  
Surface Coatings ◽  
Implant Failure ◽  
Implant Surface ◽  
Bone Implant ◽  
Cellular Behavior ◽  
Dental Implant Surface

Magnesium (Mg) is an essential trace element that has a significant role in the human body through its effects on bone metabolism. It has various applications in orthodpaedics and dentistry and the interest of this systematic review lies in its potential role as a dental implant surface coating. The dental implants can fail at different stages starting with its osseointegration phase to the restorative stage in the oral cavity. The biological loss of bone integration to the implant surface has been classified as one of the primary reasons for dental implant failure. There have been numerous strategies that have been shown to compensate this reason for implant failure, among which are the dental implant surface coatings. These coatings have been shown to improve the enhance the adhesion as well as the process of osseointegration. There are numerous studies in the existing literature that have analyzed the effects of Mg-based coatings on cellular as well as biological processes in bone-implant integration. A systematic search of various databases yielded 175 articles, of which 14 in vitro and experimental animal studies that analyzed the effect of Mg-based coatings and compared it to other coatings or no surface coatings were included in this systematic review. The main outcomes of this systematic review have been cellular behavior, osseointegration, and osteogenic markers and the effects of Mg-based coatings in these parameters have been highlighted in this review.

scholarly journals Decontamination of Dental Implant Surfaces by the Er:YAG Laser Beam: A Comparative in Vitro Study of Various Protocols

2018 ◽  
Vol 6 (4) ◽  
pp. 66 ◽  
Author(s):  
Rima Nejem Wakim ◽  
Melanie Namour ◽  
Hoang Nguyen ◽  
Andre Peremans ◽  
Toni Zeinoun ◽  
...  
Keyword(s):  
Dental Implant ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Low Energy ◽  
Vitro Study ◽  
Water Spray ◽  
Implant Surface ◽  
Dental Implant Surface ◽  
Irradiation Energy

Oral rehabilitation with dental implants has revolutionized the field of dentistry and has been proven to be an effective procedure. However, the incidence of peri-implantitis has become an emerging concern. The efficacy of the decontamination of the implant surface, by means of lasers, is still controversial. Previous studies have revealed a reduction in osteoblast adhesion to carbon-contaminated implant surfaces. This in-vitro study aimed to evaluate the decontamination of failed implants by assessing the carbon proportion, after irradiation by low-energy erbium yttrium-aluminum-garnet laser (Er:YAG) (Fotona; 2940 nm, Ljubljana, Slovenia) for a single and for multiple passages, until getting a surface, free of organic matters; to find the appropriate procedure for dental-implant surface-decontamination. Ninety implants were used. Thirty sterile implants were kept as a negative control. Thirty failed implants were irradiated by the Er:YAG laser, for a single passage, and the other thirty, for multiple passages. The parameters used in our experiments were an irradiation energy of 50 mJ, frequency of 30 Hz, and an energy density of 3.76 J/cm2. A sapphire tip, with a length of 8 mm, was used with concomitant water spray irrigation, under air 6 and water spray 4. Super short pulse mode (SSP) was of 50 μs; irradiation speed being 2 mm/s. We used energy-dispersive X-ray spectroscopy (EDX) to evaluate the carbon proportion on the surfaces of the sterile implants, the contaminated, and the lased implants, with one (LX1) and with three passages (LX3). Statistical analysis was performed by ANOVA. Results showed mean difference between the three groups (contaminated, LX1, and LX3) with p < 0.0001, as between LX1 and Group A (p < 0.0001), while the difference between LX3 and the control group was not statistically significant. The decontamination of the implant surfaces with a low-energy Er:YAG laser with three passages, appeared to be an encouraging approach.

Sign in / Sign up

Export Citation Format

Share Document