scholarly journals Cell Differentiation and Replication during Postnatal Development of the Murine First Molar

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 776
Author(s):  
Rudi Balzano ◽  
Edoardo Stellini ◽  
Carla Mucignat-Caretta
Keyword(s):  
Nuclear Antigen ◽  
Apical Region ◽  
Wild Type ◽  
Ki 67 ◽  
Tooth Formation ◽  
Odontoblast Differentiation ◽  
Dividing Cells ◽  
Cd1 Mice ◽  
Cellular Replication ◽  
Hematoxylin Eosin

Various signaling molecular pathways are involved in odontogenesis to promote cellular replication and differentiation. Tooth formation is controlled mainly by epithelial–mesenchymal interactions. The aim of this work was to investigate how cellular replication and differentiation ensue during the formation of the murine first molar in postnatal ages until eruption, focusing on morphogenesis, odontoblast differentiation and cellular replication. Wild-type CD1 mice were examined from birth to weaning. Morphogenesis and interaction between developing epithelial and mesenchymal tissues were evaluated in hematoxylin–eosin and Gomori trichome stained sections. Immunohistochemistry for nestin, which mediates the differentiation of odontoblasts, especially their polarization and elongation, showed that this intermediate filament was apparent already at postnatal day P1 in the apical region of odontoblasts and progressed apically from cusp tips, while it was not present in epithelial tissues. The expression of nuclear antigen Ki-67 highlighted dividing cells in both epithelial and mesenchymal tissues at P1, while one week later they were restricted to the cementoenamel junction, guiding root elongation. The link between odontoblast maturation and cellular replication in the different tooth tissues is essential to understand the development of tooth shape and dimension, to outline mechanisms of tooth morphogenesis and possibly eruption.

Cytoskeleton-related structures in tetrahymena thermophila: microfilaments at the apical and division-furrow rings

1981 ◽  
Vol 51 (1) ◽  
pp. 241-253
Author(s):  
M. Jerka-Dziadosz
Keyword(s):  
Cell Membrane ◽  
Type Strain ◽  
Wild Type Strain ◽  
Tetrahymena Thermophila ◽  
Apical Region ◽  
Basal Bodies ◽  
Wild Type ◽  
Fixation Procedure ◽  
Contractile Ring ◽  
Dividing Cells

A ring consisting of microfilaments was found in the apical region of Tetrahymena thermophila wild-type strain B and janus mutant. This ring, about 0.4 micrometer wide and 0.2 micrometer thick, is located at the bases of the anterior, non-ciliated basal bodies of the apical ciliary couplets. The apical ring is made of fine filaments showing a banded pattern, the distance between bands depending on the fixation procedure and ranging from 30–200 nm. The bands are made of small beads fastened to the filaments. The microfilaments of the apical ring are attached to the bases of the basel bodies. No connection with the cell membrane was found. In dividing cells in the incipient furrow region of filamentous band originates from the epiplasmic fibrogranular meshwork. This contractile ring is about 0.4 micrometer wide and 0.8 micrometer thick. It is formed by circumferentially aligned microfibrils. During constriction the contractile ring remains associated with the epiplasmic layer, which in turn adheres to the inner alveolar membrane. The microfilaments of both the apical and the division-furrow rings have diameters ranging from about 3.8-7.I nm.

Expression of Ki-67 has Correlation with the Degree and Size of Endometriosis Cysts

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Ruswana Anwar ◽  
Muhammad Alif ◽  
Adhi Pribadi
Keyword(s):  
Laparoscopic Surgery ◽  
Cell Nucleus ◽  
Significant Relationship ◽  
Strong Relationship ◽  
Cyst Size ◽  
Ki 67 ◽  
Cut Method ◽  
Close Relationship ◽  
Dividing Cells ◽  
Aggressive Tumor

Endometriosis is one of the most common gynecological problem. Cells resulted in chronic inflammation and progressive, proliferative, invasive and even infiltrating an area that resembles the character of the malignancy. Ki-67 is an antigen on the cell nucleus that is found only in actively dividing cells. Expression of Ki-67 are associated with an aggressive tumor and metastasis. This study aims to determine the level of Ki-67 expression correlation with stage and size of the endometriosis cyst. Methods research is observational analytic cross cut method on 56 paraffin blocks of patients who have been diagnosed with endometriosis and had performed a laparotomy or laparoscopic surgery in Dr Hasan Sadikin Hospital. The results showed a significant relationship between the level of expression of Ki-67 with endometriosis cyst size (p <0.001) with a fairly strong relationship (0.55) according to statistics based on criteria Guilford. Moreover the results also showed a significant relationship between the level of expression of Ki-67 with endometriosis stage (p <0.001) with a fairly close relationship (0.564) according to statistics based on criteria Guilford. It can be concluded that the expression of Ki-67 associated with cyst size and stage of endometriosis. Keywords: Ki-67, endometriosis stage, endometriosis cyst

scholarly journals Changes in Cellular Regulatory Factors before and after Decompression of Odontogenic Keratocysts

2020 ◽  
Vol 10 (1) ◽  
pp. 30
Author(s):  
Slmaro Park ◽  
Han-Sung Jung ◽  
Young-Soo Jung ◽  
Woong Nam ◽  
Jung Yul Cha ◽  
...  
Keyword(s):  
Recurrence Rate ◽  
Biological Behavior ◽  
Nuclear Antigen ◽  
Epithelial Cyst ◽  
Ki 67 ◽  
Proliferation Markers ◽  
Odontogenic Keratocysts ◽  
Wide Range ◽  
Before And After ◽  
Cyst Lining

Decompression followed by enucleation, which is one of the treatments used for odontogenic keratocysts (OKCs), is frequently used in OKC lesions of large sizes. This method offers the advantage of minimizing the possibility of sensory impairment without creating a wide-range bone defect; moreover, the recurrence rate can be significantly lower than following simple enucleation. This study aimed to assess the changes in histology and expression of proliferation markers in OKCs before and after decompression treatment. A total of 38 OKC tissue samples from 19 patients who had undergone decompression therapy were examined morphologically and immunohistochemically to observe changes in proliferative activity before and after decompression. The markers used for immunohistochemistry (IHC) staining were Bcl-2, epidermal growth factor receptor (EGFR), Ki-67, P53, PCNA, and SMO. The immunohistochemistry positivity of the 6 markers was scored by using software ImageJ, version 1.49, by quantifying the intensity and internal density of IHC-stained epithelium. The values of Bcl-2, Ki-67, P53, proliferating cell nuclear antigen (PCNA), and SMO in OKCs before and after decompression showed no significant change. No correlation between clinical shrinkage and morphologic changes or expression of proliferation and growth markers could be found. There was no statistical evidence that decompression treatment reduces potentially aggressive behavior of OKC within the epithelial cyst lining itself. This might indicate that decompression does not change the biological behavior of the epithelial cyst lining or the recurrence rate.

Haemopoiesis in Lepidoptera. III. A note on the multiplication of spherule cells and granular haemocytes

1983 ◽  
Vol 61 (1) ◽  
pp. 275-277 ◽  
Author(s):  
J. W. Arnold ◽  
C. F. Hinks
Keyword(s):  
Mitotic Index ◽  
Alcian Blue ◽  
Maximum Production ◽  
Spherule Cell ◽  
Dividing Cells ◽  
Lepidoptera Noctuidae ◽  
Potential Maximum ◽  
Hematoxylin Eosin ◽  
Cell Mitosis

Blood films from early sixth instar larvae of Euxoa declarata (Lepidoptera: Noctuidae) stained in hematoxylin – eosin – alcian blue showed unequivocal examples of mitosis in spherule cells. The improved visibility of mitosis and the estimation of the mitotic index from counts of dividing cells per 1000 cells of each type indicated a far greater potential maximum production of spherule cells and granular haemocytes by mitosis than reported previously. Certain other methods of staining showed similar clear examples of spherule cell mitosis.

Comparison of immunostainings for proliferating cell nuclear antigen and Ki-67 in human extraocular lesions

1997 ◽  
Vol 235 (12) ◽  
pp. 767-772 ◽  
Author(s):  
Tatsuo Kodama ◽  
Katsue Kawamoto ◽  
Tatsuro Kono ◽  
Yuzo Shibuya ◽  
Tomoichi Setogawa
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Ki 67 ◽  
Proliferating Cell

Mitotic Index and Ki-67 Nuclear Antigen Labeling Index as Predictors of Chemotherapy Response in Uterine Cervical Carcinoma

2001 ◽  
Vol 83 (3) ◽  
pp. 555-559 ◽  
Author(s):  
Seiryu Kamoi ◽  
Yoshiharu Ohaki ◽  
Susumu Okada ◽  
Norihiro Matsushita ◽  
Takashi Kawamura ◽  
...  
Keyword(s):  
Mitotic Index ◽  
Cervical Carcinoma ◽  
Labeling Index ◽  
Nuclear Antigen ◽  
Chemotherapy Response ◽  
Ki 67 ◽  
Uterine Cervical Carcinoma

scholarly journals Pulmonary ischemia induces lung remodeling and angiogenesis

2006 ◽  
Vol 100 (2) ◽  
pp. 587-593 ◽  
Author(s):  
Elizabeth M. Wagner ◽  
Irina Petrache ◽  
Brian Schofield ◽  
Wayne Mitzner
Keyword(s):  
Left Lung ◽  
Lung Parenchyma ◽  
Left Pulmonary Artery ◽  
Nuclear Antigen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Visceral Pleura ◽  
Artery Ligation ◽  
Dividing Cells ◽  
Apoptotic Activity ◽  
Pulmonary Ischemia

Cellular remodeling during angiogenesis in the lung is poorly described. Furthermore, it is the systemic vasculature of the lung and surrounding the lung that is proangiogenic when the pulmonary circulation becomes impaired. In a mouse model of chronic pulmonary thromboembolism, after left pulmonary artery ligation (LPAL), the intercostal vasculature, in proximity to the ischemic lung, proliferates and invades the lung ( 12 ). In the present study, we performed a detailed investigation of the kinetics of remodeling using histological sections of the left lung of C57Bl/6J mice after LPAL (4 h to 20 days) or after sham surgery. New vessels were seen within the thickened visceral pleura 4 days after LPAL predominantly in the upper portion of the left lung. Connections between new vessels within the pleura and pulmonary capillaries were clearly discerned by 7 days after LPAL. The visceral pleura and the lung parenchyma showed intense tissue remodeling, as evidenced by markedly elevated levels of both proliferating cell nuclear antigen and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling positive cells. Rapidly dividing cells were predominantly macrophages and type II pneumocytes. The increased apoptotic activity was further quantified by caspase-3 activity, which showed a sixfold increase relative to naive lungs, by 24 h after LPAL. Because sham surgeries had little effect on measured parameters, we conclude that both thoracic wound healing and pulmonary ischemia are required for systemic neovascularization.

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