scholarly journals Thymus vulgaris L. Essential Oil Solid Formulation: Chemical Profile and Spasmolytic and Antimicrobial Effects

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 860 ◽  
Author(s):  
Matteo Micucci ◽  
Michele Protti ◽  
Rita Aldini ◽  
Maria Frosini ◽  
Ivan Corazza ◽  
...  
Keyword(s):  
Essential Oil ◽  
Capillary Electrochromatography ◽  
Ex Vivo ◽  
Thymus Vulgaris ◽  
Bioactive Constituents ◽  
Liquid Chromatography Tandem Mass ◽  
Oil Formulation ◽  
Salmonella Thyphimurium ◽  
Array Detection ◽  
Different Strains

A new Thymus vulgaris L. solid essential oil (SEO) formulation composed of liquid EO linked to solid excipients has been chemically analysed and evaluated for its intestinal spasmolytic and antispastic effects in ex vivo ileum and colon of guinea pig and compared with liquid EO and excipients. Liquid EO and solid linked EO were analysed by original capillary electrochromatography coupled to diode array detection (CEC-DAD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodologies. The main bioactive constituents are thymol and carvacrol, with minor constituents for a total of 12 selected analysed compounds. Liquid EO was the most effective in decreasing basal contractility in ileum and colon; excipients addiction permitted normal contractility pattern in solid linked EO SEO. In ileum and colon, the Thymus vulgaris L. solid formulation exerted the relaxant activity on K+-depolarized intestinal smooth muscle as well as liquid EO. The solid essential oil exhibits antimicrobial activity against different strains (Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli, Salmonella Thyphimurium, Candida albicans) similarly to liquid oil, with activity against pathogen, but not commensal strains (Bifidobacterium Breve, Lactobacillus Fermentum) in intestinal homeostasis. Therefore, Thymus vulgaris L. solid essential oil formulation can be proposed as a possible spasmolytic and antispastic tool in medicine.

scholarly journals HUMIC SUBSTANCES AND MOISTURE CONTENT IN THE PRODUCTION OF BIOMASS AND BIOACTIVE CONSTITUENTS OF Th ymus vulgaris L.

2011 ◽  
Vol 34 (3) ◽  
pp. 183
Author(s):  
Cecilia R. Juárez R. ◽  
Lyle E. Craker ◽  
Ma. de las Nieves Rodríguez-Mendoza ◽  
Juan A. Aguilar-Castillo
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Humic Substances ◽  
Direct Interaction ◽  
Field Capacity ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Bioactive Constituents ◽  
Positive Effects ◽  
Phenolic And Flavonoid

Thyme (Thymus vulgaris L.) is an aromatic and medicinal plant with antioxidant, antimicrobial and antinfungical properties that have attracted the interest of pharmacological and food industries. Humic substances (HS) are a natural product that has shown beneficial effects on soil and on plant growth. These positive effects are explained by the direct interaction of HS with physiological processes. But it is unknown their effect on the composition of the thyme bioactive constituents. To test the effect of humic substances, seeds of T. vulgaris were germinated and grown at different rates of HS (100, 200, 300 and 400 mg L-1). Th yme plants were also grown at three levels of moisture (20, 40 and 60 % of field capacity). At harvest plant height and shoot fresh and dry weight were measured. Bioactive constituents were determined in terms of yield and principal components of essential oil (by distillation in Clevenger apparatus and gas chromatography). Antioxidant activity, total phenolic and flavonoid content were determined with spectrophotometric techniques. Morphological variables, except for shoot fresh weight, showed signifi cant differences (P ≤ 0.05) among treatments. The essential oil yield and composition, as well as antioxidant activity and total phenolic and flavonoid contents, were higher at the highest levels of HS and substrate moisture.

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

Diterpenoid and phenolic contents of Sideritis hololeuca Boiss & Heldr. Apud Bentham with antioxidant and anticholinesterase activity

2020 ◽  
Vol 75 (5-6) ◽  
pp. 161-169
Author(s):  
Sema Carikci ◽  
Ahmet C. Goren ◽  
Turgut Kilic
Keyword(s):  
Essential Oil ◽  
Biological Activities ◽  
Reducing Power ◽  
Acetone Extract ◽  
Inhibition Activity ◽  
Phenolic Contents ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Main Component ◽  
Butyrylcholinesterase Inhibition

AbstractThe objective of this study is to determine essential oil, diterpenoid and phenolic contents of Sideritis hololeuca along with their biological activities. Phytol was found to be the main component of the essential oil. Seven known kaurane diterpenoids, siderol (140 mg, 0.16%, w/w), 7-acetoxy sideroxol (15 mg, 0.02%, w/w), eubol (6 mg, 0.01%, w/w), eubotriol (5 mg, 0.03%, w/w), 7-epicandicandiol (3 mg, 0.02%, w/w), ent-7α-acetoxy-18-hydroxykaur-16-ene (5 mg, 0.01%, w/w) and linearol [by liquid chromatography-tandem mass spectrometry (LC-MS/MS)] were determined from the species. Moreover, vanillin (21 mg, 0.10%, w/w), which was not isolated from any Sideritis species earlier, was isolated from an acetone extract of S. hololeuca. Quantitative amounts of some phenolic compounds in n-hexane, dichloromethane, acetone, methanol extracts and infusion and decoction of the plants were also investigated by LC-MS/MS. Antioxidant capacity and acetylcholinesterase, butyrylcholinesterase inhibition effects of the species were evaluated. The extracts of methanol and infusion and decoction of species showed moderate butyrylcholinesterase inhibition activity. The highest inhibition was observed from the decoction of species. The Cu2+ reducing power of infusion was determined as 1.435 mmol TR g−1.

scholarly journals Myorelaxant Effect of the Dysphania ambrosioides Essential Oil on Sus scrofa domesticus Coronary Artery and Its Toxicity in the Drosophila melanogaster Model

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2041
Author(s):  
Luiz Jardelino de Lacerda Neto ◽  
Andreza Guedes Barbosa Ramos ◽  
Renata Evaristo Rodrigues da Silva ◽  
Luís Pereira-de-Morais ◽  
Fernanda Maria Silva ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Essential Oil ◽  
Coronary Arteries ◽  
Sus Scrofa ◽  
Ex Vivo ◽  
Alternative Methods ◽  
Major Constituent ◽  
Sus Scrofa Domesticus

Purpose: Alternative methods for the use of animals in research have gained increasing importance, due to assessments evaluating the real need for their use and the development of legislation that regulates the subject. The principle of the 3R’s (replacement, reduction and refinement) has been an important reference, such that in vitro, ex vivo and cord replacement methods have achieved a prominent place in research. Methods: Therefore, due to successful results from studies developed with these methods, the present study aimed to evaluate the myorelaxant effect of the Dysphania ambrosioides essential oil (EODa) using a Sus scrofa domesticus coronary artery model, and the toxicity of both the Dysphania ambrosioides essential oil and its major constituent, α-terpinene, against Drosophila melanogaster in toxicity and negative geotaxis assays. Results: The EODa relaxed the smooth muscle of swine coronary arteries precontracted with K+ and 5-HT in assays using Sus scrofa domesticus coronary arteries. The toxicity results presented LC50 values of 1.546 mg/mL and 2.282 mg/mL for the EODa and α-terpinene, respectively, thus showing the EODa and α-terpinene presented toxicity to these dipterans, with the EODa being more toxic. Conclusions: Moreover, the results reveal the possibility of using the EODa in vascular disease studies since it promoted the relaxation of the Sus scrofa domesticus coronary smooth muscle.

Potencial do óleo essencial de Thymus vulgaris na qualidade sanitária em sementes de Caesalpinia férrea/ Essential oil potential Thymus vulgaris on the health quality Caesalpinia ferrea seeds

2021 ◽  
Vol 7 (7) ◽  
pp. 66772-66785
Author(s):  
Andréa Celina Ferreira Demartelaere ◽  
Pablo Wenderson Ribeiro Coutinho ◽  
Hailson Alves Ferreira Preston ◽  
José George Ferreira Medeiros ◽  
Jaltiery Bezerra de Souza ◽  
...  
Keyword(s):  
Essential Oil ◽  
Thymus Vulgaris ◽  
Health Quality

scholarly journals Novel polymeric micelles for insect pest control: encapsulation of essential oil monoterpenes inside a triblock copolymer shell for head lice control

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3171 ◽  
Author(s):  
Alejandro Lucia ◽  
Ariel Ceferino Toloza ◽  
Eduardo Guzmán ◽  
Francisco Ortega ◽  
Ramón G. Rubio
Keyword(s):  
Essential Oil ◽  
Pest Control ◽  
Polymeric Micelles ◽  
Ex Vivo ◽  
Insect Pest ◽  
Immersion Test ◽  
Poloxamer 407 ◽  
Head Lice ◽  
Hydrophobic Core ◽  
Micellar Systems

BackgroundEssential oil components (EOCs) are molecules with interesting application in pest control, these have been evaluated against different insect pest from more than 100 years, but their practical use is rather limited. Thus, the enhancement of their bioavailability and manageability due to their dispersion in water can open new perspective for the preparation of formulations for the control of insect pest. In this work, we studied the encapsulation of different monoterpenes in a poloxamer shell in order to prepare aqueous formulations that can be used for the development of platforms used in pest control.MethodsMicellar systems containing a 5 wt% of poloxamer 407 and 1.25 wt% of the different monoterpenes were prepared. Dynamic Light Scattering (DLS) experiments were carried out to characterize the dispersion of the EOCs in water. The pediculicidal activity of these micellar systems was tested on head lice using anex vivoimmersion test.ResultsThe poloxamers allowed the dispersion of EOCs in water due to their encapsulation inside the hydrophobic core of the copolymer micelles. From this study, we concluded that it is possible to make stable micellar systems containing water (>90 wt%), 1.25 wt% of different monoterpenes and a highly safe polymer (5wt% Poloxamer 407). These formulations were effective against head lice with mortality ranging from 30 to 60%, being the most effective emulsions those containing linalool, 1,8-cineole,α-terpineol, thymol, eugenol, geraniol and nonyl alcohol which lead to mortalities above 50%.DiscussionSince these systems showed good pediculicidal activity and high physicochemical stability, they could be a new route for the green fabrication of biocompatible and biosustainable insecticide formulations.

Bioactive constituents of Homalomena aromatica essential oil and its antifungal activity against dermatophytes and yeasts

2012 ◽  
Vol 22 (1) ◽  
pp. 83-87 ◽  
Author(s):  
R.S. Policegoudra ◽  
S. Goswami ◽  
S.M. Aradhya ◽  
S. Chatterjee ◽  
S. Datta ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Bioactive Constituents
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