scholarly journals 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1717
Author(s):  
Silvia Ravera ◽  
Nadia Bertola ◽  
Claudio Pasquale ◽  
Silvia Bruno ◽  
Stefano Benedicenti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Squamous Cell Carcinoma ◽  
Energy Metabolism ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Continuous Wave ◽  
Neck Squamous Cell Carcinoma ◽  
Antioxidant Defenses ◽  
Cellular Model

Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-malignant cells remain unknown. Thus, the primary endpoint was to assess the safety of PBM treatment parameters on head and neck squamous cell carcinoma (HNSCC) proliferation or survival. The secondary endpoint was to assess any putative anti-cancer effects of PBM treatments. Cell viability, energy metabolism, oxidative stress, and pro- and anti-apoptotic markers expression were investigated on a Human Head and Neck Squamous Cell Carcinoma cellular model (OHSU-974 FAcorr cell line). PBM therapy was administered through the 810 nm diode laser (GaAlAs) device (Garda Laser, 7024 Negrar, Verona, Italy) at the powers of 0, 0.25, 0.50, 0.75, 1.00, or 1.25 W in continuous wave (CW) mode for an exposure time of 60 s with a spot-size of 1 cm2 and with a distance of 1.86 cm from the cells. Results showed that 810-nm PBM affected oxidative phosphorylation in OHSU-971 FAcorr, causing a metabolic switch to anaerobic glycolysis. In addition, PBM reduced the catalase activity, determining an unbalance between oxidative stress production and the antioxidant defenses, which could stimulate the pro-apoptotic cellular pathways. Our data, at the parameters investigated, suggest the safeness of PBM as a supportive cancer therapy. Pre-clinical and clinical studies are necessary to confirm the in vitro evidence.

scholarly journals Acquisition of Cisplatin Resistance Shifts Head and Neck Squamous Cell Carcinoma Metabolism toward Neutralization of Oxidative Stress

Cancers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1670 ◽  
Author(s):  
Wangjie Yu ◽  
Yunyun Chen ◽  
Nagireddy Putluri ◽  
Cristian Coarfa ◽  
Matthew J. Robertson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Cisplatin Resistance ◽  
Integrated Approach ◽  
Metabolic Reprogramming ◽  
Neck Squamous Cell Carcinoma ◽  
Cross Resistance

Background: Cisplatin (CDDP) is commonly utilized in the treatment of advanced solid tumors including head and neck squamous cell carcinoma (HNSCC). Cisplatin response remains highly variable among individual tumors and development of cisplatin resistance is common. We hypothesized that development of cisplatin resistance is partially driven by metabolic reprogramming. Methods: Using a pre-clinical HNSCC model and an integrated approach to steady state metabolomics, metabolic flux and gene expression data we characterized the interaction between cisplatin resistance and metabolic reprogramming. Results: Cisplatin toxicity in HNSCC was driven by generation of intra-cellular oxidative stress. This was validated by demonstrating that acquisition of cisplatin resistance generates cross-resistance to ferroptosis agonists despite the fact that cisplatin itself does not trigger ferroptosis. Acquisition of cisplatin resistance dysregulated the expression of genes involved in amino acid, fatty acid metabolism and central carbon catabolic pathways, enhanced glucose catabolism and serine synthesis. Acute cisplatin exposure increased intra-tumoral levels of S-methyl-5-thiadenosine (MTA) precursors and metabotoxins indicative of generalized oxidative stress. Conclusions: Acquisition of cisplatin resistance is linked to metabolic recovery from oxidative stress. Although this portends poor effectiveness for directed metabolic targeting, it supports the potential for biomarker development of cisplatin effectiveness using an integrated approach.

Tumor Tissue Oxidative Stress Changes and Na, K-ATPase Evaluation in Head and Neck Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Marina Marques Toledo ◽  
Bruno De Souza Gonçalves ◽  
Natalie Mounteer Colodette ◽  
Aline Lauda Freitas Chaves ◽  
Luciana Vieira Muniz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Tumor Tissue ◽  
Neck Squamous Cell Carcinoma ◽  
Stress Changes

Assessment of oxidative stress in tumors and histologically normal mucosa from patients with head and neck squamous cell carcinoma

2013 ◽  
Vol 22 (6) ◽  
pp. 558-560 ◽  
Author(s):  
Didier Dequanter ◽  
Maureen Van de Velde ◽  
Vincent Nuyens ◽  
Nathalie Nagy ◽  
Paul Van Antwerpen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Normal Mucosa ◽  
Neck Squamous Cell Carcinoma

scholarly journals Lipid Peroxidation and Antioxidant Status in Head and Neck Squamous Cell Carcinoma Patients

2009 ◽  
Vol 2 (2) ◽  
pp. 68-72 ◽  
Author(s):  
Aashita Gupta ◽  
Madan L. B. Bhatt ◽  
Mithilesh K. Misra
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Squamous Cell Carcinoma ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Patients ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma

Oxidative stress, a consequence of an imbalance between the formation and inactivation of reactive oxygen species, may be involved in the pathogenesis of many diseases including cancer. To evaluate the magnitude of oxidative stress, a study on the plasma levels of superoxide dismutase, total thiols, ascorbic acid and malondialdehyde (MDA) has been done in head and neck squamous cell carcinoma patients before the start of any oncological treatment and compared with healthy controls. The specific activity of superoxide dismutase in cancer patients is decreased significantly when compared to the control (p < 0.05). The total thiol and ascorbic acid levels are significantly reduced (p < 0.005) whereas MDA levels are significantly increased in the patients (p < 0.00005). Our findings show that the oxidative stress is elevated in cancer patients as evidenced by elevated levels of lipid peroxidation products and depletion of enzymatic and non-enzymatic antioxidants.

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