scholarly journals Cytotoxicity of Different Composite Resins on Human Gingival Fibroblast Cell Lines

Biomimetics ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 26
Author(s):  
Riccardo Beltrami ◽  
Marco Colombo ◽  
Keren Rizzo ◽  
Alessio Di Cristofaro ◽  
Claudio Poggio ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Composite Resins ◽  
Wilcoxon Test ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Viable Cells

The aim of the present study was to evaluate and compare the cytotoxic effects of eight composite resins on immortalized human gingival fibroblasts. Composite resins were eluted in cell culture medium for 48 or 72 h at 37 °C. Immortalized human gingival fibroblast-1 (HGF-1) cell lines were seeded in 96-well (1 × 104) plates and incubated for 24 h at 37 °C with the obtained extraction medium. The percentage of viable cells in each well (MTT test) was calculated relative to control cells, which were set to 100%. Data observed were not normally distributed, and nonparametric statistical methods were used for statistical analysis. The Wilcoxon test was used for intragroup comparison, and the Kruskal–Wallis test was used for intergroup multiple comparisons. Significance value was set as p < 0.05. All materials tested showed cytotoxic effects on gingival fibroblasts, recordable as noncytotoxic, mildly cytotoxic or severely cytotoxic, depending on the percentage of cell viability. The Wilcoxon test for intragroup comparison showed that the percentage of viable cells decreased significantly for extracts, for all composite resins tested. The composite resins contained monomers that displayed cytotoxic properties. BisGMA, TEGDMA and UDMA had inhibitory effects and induced apoptotic proteins in pulp fibroblast. Composite resins that contained lower percentages of unbound free monomers—and that released less ions—possessed superior biocompatibility in vitro.

Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

1978 ◽  
Vol 57 (11-12) ◽  
pp. 1003-1015 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cover Slip ◽  
Fibroblast Cell Lines

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (1) ◽  
pp. 53-70 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals Cytotoxicity of Different Nano Composite Resins on Human Gingival and Periodontal Ligament Fibroblast Cell Lines: An In Vitro Study

Biomedicines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 48 ◽  
Author(s):  
Gamze Kavuncu ◽  
Ayse Mine Yilmaz ◽  
Betul Karademir Yilmaz ◽  
Pinar Yilmaz Atali ◽  
Elif Cigdem Altunok ◽  
...  
Keyword(s):  
Cell Lines ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Composite Resins ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Nano Composite ◽  
Periodontal Ligament Fibroblast

The aim of this study is to determine the cytotoxicity of three different nano composite resins (CRs) on human gingival fibroblast (hGF) and periodontal ligament fibroblast (hPDLF) cell lines. These CRs selected were nanohybrid organic monomer-based Admira Fusion (AF), nanohybrid Bis-(acryloyloxymethyl) tricyclo [5.2.1.0.sup.2,6] decane-based Charisma Topaz (CT), and supra nano filled resin-based Estelite Quick Sigma (EQS). MTT assay was performed to assess the cytotoxicity of CRs at 24 h and one week. AF and EQS applied on hGF cells at 24 h and one week demonstrated similar cytotoxic outcomes. Cytotoxicity of CT on hGF cells at one week was higher than 24 h (p = 0.04). Cytotoxicity of CT on hGF cells was higher at 24 h (p = 0.002) and one week (p = 0.009) compared to control. All composites showed higher cytotoxicity on hPDLF cells at one week than the 24 h (AF; p = 0.02, CT; p = 0.02, EQS; p = 0.04). AF and EQS demonstrated lower cytotoxicity on hPDLF cells than the control group at 24 h (AF; p = 0.01, EQS; p = 0.001). CT was found more cytotoxic on hPDLF cells than the control (p = 0.01) and EQS group (p = 0.008) at one week. The cytotoxicity of CRs on hGF and hPDLF cells vary, according to the type of composites, cell types, and exposure time.

Human gingival fibroblast cell lines in vitro.

1980 ◽  
Vol 15 (3) ◽  
pp. 267-287 ◽  
Author(s):  
Toshihiko Yajima ◽  
George G. Rose ◽  
Charles J. Mahan
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals Cytotoxic Effects of Pistacia Atlantica (Baneh) Fruit Extract on Human KB Cancer Cell Line

2019 ◽  
Vol 62 (1) ◽  
pp. 30-34
Author(s):  
Zohreh Jaafari-Ashkvandi ◽  
Saeed Yousefi Shirazi ◽  
Somayeh Rezaeifard ◽  
Azadeh Hamedi ◽  
Nasrollah Erfani
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Normal Fibroblast ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Dependent Manner ◽  
Apoptosis Induction ◽  
Cytotoxic Effects ◽  
Induction Of Apoptosis

Plants with anticancer properties are considered as cancer preventive and treatment sources, due to their some biological effects. Apoptosis induction and anti-proliferative effects of Baneh extract on various cancer cell lines have been reported. Hence, this study was designed to evaluate the cytotoxic effects of this fruit on KB and human gingival fibroblast cell lines (HGF). KB and HGF cells were treated with various concentrations of ethanolic Baneh extract and cisplatin as positive control. Cytotoxic activity and apoptosis induction were investigated using WST-1 and Annexin V assays. Data were analyzed using ANOVA and student’s t-tests. IC50 after 24 and 48 hours treatment were respectively 2.6 and 1 mg/mL for KB cell line, and 1.5 and 1.6 mg/mL for HGF cell. During 48 hours Baneh extract induced apoptosis without significant necrosis, in a time- and dose-dependent manner. The induction of apoptosis in KB cells was significantly higher than HGF. It seems that ethanolic extract of Baneh contains compounds that can suppress KB cell growth through the induction of apoptosis. Within 48 hours, less cytotoxic effects were observed on normal fibroblast cells; therefore, it might be a potential anticancer agent.

scholarly journals Comparison of Cytomorphometry and Early Cell Response of Human Gingival Fibroblast (HGFs) between Zirconium and New Zirconia-Reinforced Lithium Silicate Ceramics (ZLS)

2018 ◽  
Vol 19 (9) ◽  
pp. 2718 ◽  
Author(s):  
María Rizo-Gorrita ◽  
Irene Luna-Oliva ◽  
María-Ángeles Serrera-Figallo ◽  
José-Luis Gutiérrez-Pérez ◽  
Daniel Torres-Lagares
Keyword(s):  
Cellular Response ◽  
Surface Characterization ◽  
Cellular Proliferation ◽  
Lithium Silicate ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Optical Profilometry

New zirconia-reinforced lithium silicate ceramics (ZLS) could be a viable alternative to zirconium (Y-TZP) in the manufacture of implantological abutments—especially in aesthetic cases—due to its good mechanical, optical, and biocompatibility properties. Although there are several studies on the ZLS mechanical properties, there are no studies regarding proliferation, spreading, or cytomorphometry. We designed the present study which compares the surface, cellular proliferation, and cellular morphology between Y-TZP (Vita YZ® T [Vita Zahnfabrik (Postfach, Germany)]) and ZLS (Celtra® Duo [Degudent (Hanau-Wolfgang, Germany)]). The surface characterization was performed with energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), and optical profilometry. Human gingival fibroblasts (HGFs) were subsequently cultured on both materials and early cellular response and cell morphology were compared through nuclear and cytoskeletal measurement parameters using confocal microscopy. The results showed greater proliferation and spreading on the surface of Y-TZP. This could indicate that Y-TZP continues to be a gold standard in terms of transgingival implant material: Nevertheless, more in vitro and in vivo research is necessary to confirm the results obtained in this study.

Establishment of immotalized human gingival fibroblast cell lines

2002 ◽  
Vol 32 (3) ◽  
pp. 603
Author(s):  
Jae-Bong Song ◽  
Hyun-A Kim ◽  
Ha-Na Hyun ◽  
Eun-Cheol Kim ◽  
Hyung-Keun You ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals Cytotoxicity Evaluation of Two Bis-Acryl Composite Resins Using Human Gingival Fibroblasts

2016 ◽  
Vol 27 (5) ◽  
pp. 492-496 ◽  
Author(s):  
Fabiano Palmeira Gonçalves ◽  
◽  
Gutemberg Alves ◽  
Vladi Oliveira Guimarães Júnior ◽  
Marco Antônio Gallito ◽  
...  
Keyword(s):  
Cell Viability ◽  
Membrane Integrity ◽  
Composite Resins ◽  
Human Gingival Fibroblasts ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Xtt Assay

Abstract Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even when cells were exposed to extracts prepared with longer conditioning times, the bis-acryl composite resins showed no significant cytotoxic effects (p>0.05), compared to the control group or in relation to the first 24 h of contact with the products. There were no differences among the results obtained for the bis-acryl composite resins evaluated 24 h, 7 days and 40 days after mixing. It may be concluded that the bis-acryl resins Protemp 4 and Luxatemp Star were cytocompatible with human gingival fibroblasts, suggesting that both materials are suitable for use in contact with human tissues.

scholarly journals Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report

2020 ◽  
Author(s):  
Barbara Sterczała ◽  
Kinga Grzech-Leśniak ◽  
Olga Michel ◽  
Witold Trzeciakowski ◽  
Kamil Jurczyszyn
Keyword(s):  
Energy Density ◽  
Preliminary Report ◽  
Human Fibroblasts ◽  
Mitochondrial Activity ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Proliferation ◽  
Gingival Fibroblasts ◽  
Healthy Donors

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types: Nd:YAG (1064nm), infrared diode laser (980nm) and prototype led laser emitting 405, 450 and 635nm were used to irradiate fibroblasts. Thanks to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm&sup2;, 25 J/cm&sup2;, 64 J/cm&sup2;. The viability and proliferation of cells were determined using the MTT test conducted 24, 48 and 72 hours after laser irradiation. Results: The highest percentage of mitochondrial activity (MA=122.1%) was observed in the group irradiated with the 635nm laser, with an energy density of 64 J/cm&sup2; after 48 hours. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405nm laser at 25 J/cm&sup2; gave similar results to the 635 nm laser. Conclusions: The application of the 635nm and 405nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.

scholarly journals Cytokine Responses to Treponema pectinovorum andTreponema denticola in Human Gingival Fibroblasts

2000 ◽  
Vol 68 (9) ◽  
pp. 5284-5292 ◽  
Author(s):  
Connie S. Nixon ◽  
Michelle J. Steffen ◽  
Jeffrey L. Ebersole
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Cytokine Production ◽  
Inflammatory Responses ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cytokine Profiles ◽  
Fibroblast Cell Lines

ABSTRACT Human gingival fibroblasts were challenged with Treponema pectinovorum and Treponema denticola to test three specific hypotheses: (i) these treponemes induce different cytokine profiles from the fibroblasts, (ii) differences in cytokine profiles are observed after challenge with live versus killed treponemes, and (iii) differences in cytokine profiles are noted from different gingival fibroblast cell lines when challenged with these treponemes. Three normal gingival fibroblast cell cultures were challenged withT. pectinovorum and T. denticola strains, and the supernatants were analyzed for cytokine production (i.e., interleukin-1α [IL-1α], IL-1β, IL-6, IL-8, IL-10, gamma interferon, macrophage chemotactic protein 1 [MCP-1], platelet-derived growth factor, tumor necrosis factor alpha, and granulocyte-macrophage colony-stimulating factor). Unstimulated fibroblast cell lines produced IL-6, IL-8, and MCP-1. T. pectinovorum routinely elicited the greatest production of these cytokines from the fibroblast cell lines, increasing 10- to 50-fold over basal production. While T. denticola also induced IL-6 and IL-8 production, these levels were generally lower than those elicited by challenge with T. pectinovorum. MCP-1 levels were significantly lower after T. denticola challenge, and the kinetics suggested that this microorganism actually inhibited basal production by the fibroblasts. No basal or stimulated production of the other cytokines was observed. Significant differences were noted in the responsiveness of the various cell lines with respect to the two species of treponemes and the individual cytokines produced. Finally, dead T. pectinovorum generally induced a twofold-greater level of IL-6 and IL-8 than the live bacteria. These results supported the idea that different species of oral treponemes can elicit proinflammatory cytokine production by gingival cells and that this stimulation did not require live microorganisms. Importantly, a unique difference was noted in the ability of T. pectinovorum to induce a robust MCP-1 production, while T. denticolaappeared to inhibit this activity of the fibroblasts. While the general cytokine profiles of the fibroblast cell cultures were similar, significant differences were noted in the quantity of individual cytokines produced, which could relate to individual patient variation in local inflammatory responses in the periodontium.

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