Comparison of Cytomorphometry and Early Cell Response of Human Gingival Fibroblast (HGFs) between Zirconium and New Zirconia-Reinforced Lithium Silicate Ceramics (ZLS)

New zirconia-reinforced lithium silicate ceramics (ZLS) could be a viable alternative to zirconium (Y-TZP) in the manufacture of implantological abutments—especially in aesthetic cases—due to its good mechanical, optical, and biocompatibility properties. Although there are several studies on the ZLS mechanical properties, there are no studies regarding proliferation, spreading, or cytomorphometry. We designed the present study which compares the surface, cellular proliferation, and cellular morphology between Y-TZP (Vita YZ® T [Vita Zahnfabrik (Postfach, Germany)]) and ZLS (Celtra® Duo [Degudent (Hanau-Wolfgang, Germany)]). The surface characterization was performed with energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), and optical profilometry. Human gingival fibroblasts (HGFs) were subsequently cultured on both materials and early cellular response and cell morphology were compared through nuclear and cytoskeletal measurement parameters using confocal microscopy. The results showed greater proliferation and spreading on the surface of Y-TZP. This could indicate that Y-TZP continues to be a gold standard in terms of transgingival implant material: Nevertheless, more in vitro and in vivo research is necessary to confirm the results obtained in this study.

Download Full-text

Early Biofilm Formation on UV Light Activated Nanoporous TiO2SurfacesIn Vivo

International Journal of Biomaterials ◽

10.1155/2018/7275617 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Nagat Areid ◽

Eva Söderling ◽

Johanna Tanner ◽

Ilkka Kangasniemi ◽

Timo O. Närhi

Keyword(s):

Titanium Alloy ◽

Biofilm Formation ◽

Uv Light ◽

Antibacterial Properties ◽

Mutans Streptococci ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Biofilm Development

Purpose. To explore earlyS. mutansbiofilm formation on hydrothermally induced nanoporous TiO2surfacesin vivoand to examine the effect of UV light activation on the biofilm development.Materials and Methods. Ti-6Al-4V titanium alloy discs (n = 40) were divided into four groups with different surface treatments: noncoated titanium alloy (NC); UV treated noncoated titanium alloy (UVNC); hydrothermally induced TiO2coating (HT); and UV treated titanium alloy with hydrothermally induced TiO2coating (UVHT).In vivoplaque formation was studied in 10 healthy, nonsmoking adult volunteers. Titanium discs were randomly distributed among the maxillary first and second molars. UV treatment was administered for 60 min immediately before attaching the discs in subjects’ molars. Plaque samples were collected 24h after the attachment of the specimens. Mutans streptococci (MS), non-mutans streptococci, and total facultative bacteria were cultured, and colonies were counted.Results. The plaque samples of NC (NC + UVNC) surfaces showed over 2 times more oftenS. mutanswhen compared to TiO2surfaces (HT + UVHT), with the number of colonized surfaces equal to 7 and 3, respectively.Conclusion. Thisin vivostudy suggested that HT TiO2surfaces, which we earlier showed to improve blood coagulation and encourage human gingival fibroblast attachmentin vitro, do not enhance salivary microbial (mostly mutans streptococci) adhesion and initial biofilm formation when compared with noncoated titanium alloy. UV light treatment provided Ti-6Al-4V surfaces with antibacterial properties and showed a trend towards less biofilm formation when compared with non-UV treated titanium surfaces.

Download Full-text

Gene Profiling in Human Periodontal Ligament Fibroblasts by Subtractive Hybridization

Journal of Dental Research ◽

10.1177/154405910308200814 ◽

2003 ◽

Vol 82 (8) ◽

pp. 641-645 ◽

Cited By ~ 7

Author(s):

T. Yamamoto ◽

F. Myokai ◽

F. Nishimura ◽

T. Ohira ◽

N. Shiomi ◽

...

Keyword(s):

Mrna Expression ◽

Periodontal Ligament ◽

Subtractive Hybridization ◽

Gene Profiling ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Periodontal Ligament Fibroblasts ◽

Human Periodontal Ligament Fibroblasts

Genes expressed by human periodontal ligament fibroblasts (HPFs) are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 × 103 pfu) was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes, including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29, identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.

Download Full-text

Negative Control of the Myc Protein by the Stress-Responsive Kinase Pak2

Molecular and Cellular Biology ◽

10.1128/mcb.24.4.1582-1594.2004 ◽

2004 ◽

Vol 24 (4) ◽

pp. 1582-1594 ◽

Cited By ~ 53

Author(s):

Zhongdong Huang ◽

Jolinda A. Traugh ◽

J. Michael Bishop

Keyword(s):

Cellular Response ◽

Cellular Proliferation ◽

Suppressor Gene ◽

Negative Control ◽

Negative Regulator ◽

Serine Threonine Kinase ◽

Response To Stress ◽

Nih 3T3

ABSTRACT Pak2 is a serine/threonine kinase that participates in the cellular response to stress. Among the potential substrates for Pak2 is the protein Myc, encoded by the proto-oncogene MYC. Here we demonstrate that Pak2 phosphorylates Myc at three sites (T358, S373, and T400) and affects Myc functions both in vitro and in vivo. Phosphorylation at all three residues reduces the binding of Myc to DNA, either by blocking the requisite dimerization with Max (through phosphorylation at S373 and T400) or by interfering directly with binding to DNA (through phosphorylation at T358). Phosphorylation by Pak2 inhibits the ability of Myc to activate transcription, to sustain cellular proliferation, to transform NIH 3T3 cells in culture, and to elicit apoptosis on serum withdrawal. These results indicate that Pak2 is a negative regulator of Myc, suggest that inhibition of Myc plays a role in the cellular response to stress, and raise the possibility that Pak2 may be the product of a tumor suppressor gene.

Download Full-text

Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report

10.20944/preprints202010.0600.v1 ◽

2020 ◽

Author(s):

Barbara Sterczała ◽

Kinga Grzech-Leśniak ◽

Olga Michel ◽

Witold Trzeciakowski ◽

Kamil Jurczyszyn

Keyword(s):

Energy Density ◽

Preliminary Report ◽

Human Fibroblasts ◽

Mitochondrial Activity ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Proliferation ◽

Gingival Fibroblasts ◽

Healthy Donors

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types: Nd:YAG (1064nm), infrared diode laser (980nm) and prototype led laser emitting 405, 450 and 635nm were used to irradiate fibroblasts. Thanks to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm², 25 J/cm², 64 J/cm². The viability and proliferation of cells were determined using the MTT test conducted 24, 48 and 72 hours after laser irradiation. Results: The highest percentage of mitochondrial activity (MA=122.1%) was observed in the group irradiated with the 635nm laser, with an energy density of 64 J/cm² after 48 hours. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405nm laser at 25 J/cm² gave similar results to the 635 nm laser. Conclusions: The application of the 635nm and 405nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.

Download Full-text

Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

Journal of Dental Research ◽

10.1177/00220345780570110101 ◽

1978 ◽

Vol 57 (11-12) ◽

pp. 1003-1015 ◽

Cited By ~ 16

Author(s):

George G. Rose ◽

Toshihiko Yajima ◽

Charles J. Mahan

Keyword(s):

Tissue Culture ◽

Thin Layer ◽

Cell Lines ◽

Fibroblast Cell ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Gingival Fibroblasts ◽

Cover Slip ◽

Fibroblast Cell Lines

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

Download Full-text

Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report

Journal of Personalized Medicine ◽

10.3390/jpm11020098 ◽

2021 ◽

Vol 11 (2) ◽

pp. 98

Author(s):

Barbara Sterczała ◽

Kinga Grzech-Leśniak ◽

Olga Michel ◽

Witold Trzeciakowski ◽

Marzena Dominiak ◽

...

Keyword(s):

Energy Density ◽

Human Fibroblasts ◽

Mitochondrial Activity ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Proliferation ◽

Gingival Fibroblasts ◽

Healthy Donors ◽

Thiazolyl Blue Tetrazolium

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types, Nd:YAG (1064 nm), infrared diode laser (980 nm), and prototype led laser emitting 405, 450, and 635 nm were used to irradiate the fibroblasts. Due to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm2, 25 J/cm2, 64 J/cm2. The viability and proliferation of cells were determined using the (Thiazolyl Blue Tetrazolium Blue) (MTT) test conducted 24, 48, and 72 h after laser irradiation. Results: The highest percentage of mitochondrial activity (MA = 122.1%) was observed in the group irradiated with the 635 nm laser, with an energy density of 64 J/cm2 after 48 h. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405 nm laser at 25 J/cm2 gave similar results to the 635 nm laser. Conclusions: The application of the 635 nm and 405 nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.

Download Full-text

Cytotoxicity of Different Composite Resins on Human Gingival Fibroblast Cell Lines

Biomimetics ◽

10.3390/biomimetics6020026 ◽

2021 ◽

Vol 6 (2) ◽

pp. 26

Author(s):

Riccardo Beltrami ◽

Marco Colombo ◽

Keren Rizzo ◽

Alessio Di Cristofaro ◽

Claudio Poggio ◽

...

Keyword(s):

Cell Lines ◽

Fibroblast Cell ◽

Composite Resins ◽

Wilcoxon Test ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Gingival Fibroblasts ◽

Cytotoxic Effects ◽

Viable Cells

The aim of the present study was to evaluate and compare the cytotoxic effects of eight composite resins on immortalized human gingival fibroblasts. Composite resins were eluted in cell culture medium for 48 or 72 h at 37 °C. Immortalized human gingival fibroblast-1 (HGF-1) cell lines were seeded in 96-well (1 × 104) plates and incubated for 24 h at 37 °C with the obtained extraction medium. The percentage of viable cells in each well (MTT test) was calculated relative to control cells, which were set to 100%. Data observed were not normally distributed, and nonparametric statistical methods were used for statistical analysis. The Wilcoxon test was used for intragroup comparison, and the Kruskal–Wallis test was used for intergroup multiple comparisons. Significance value was set as p < 0.05. All materials tested showed cytotoxic effects on gingival fibroblasts, recordable as noncytotoxic, mildly cytotoxic or severely cytotoxic, depending on the percentage of cell viability. The Wilcoxon test for intragroup comparison showed that the percentage of viable cells decreased significantly for extracts, for all composite resins tested. The composite resins contained monomers that displayed cytotoxic properties. BisGMA, TEGDMA and UDMA had inhibitory effects and induced apoptotic proteins in pulp fibroblast. Composite resins that contained lower percentages of unbound free monomers—and that released less ions—possessed superior biocompatibility in vitro.

Download Full-text

Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix

Brazilian Dental Journal ◽

10.1590/s0103-64402010000300001 ◽

2010 ◽

Vol 21 (3) ◽

pp. 179-189 ◽

Cited By ~ 12

Author(s):

Annelissa Zorzeto Rodrigues ◽

Paulo Tambasco de Oliveira ◽

Arthur Belém Novaes Jr. ◽

Luciana Prado Maia ◽

Sérgio Luís Scombatti de Souza ◽

...

Keyword(s):

Cell Monolayer ◽

Acellular Dermal Matrix ◽

Human Gingival Fibroblasts ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Gingival Fibroblasts ◽

Dermal Matrix ◽

The Matrix ◽

Acellular Dermal

The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.

Download Full-text

The Radiation-Induced Regenerative Response of Adult Tissue-Specific Stem Cells: Models and Signaling Pathways

Cancers ◽

10.3390/cancers13040855 ◽

2021 ◽

Vol 13 (4) ◽

pp. 855

Author(s):

Paola Serrano Martinez ◽

Lorena Giuranno ◽

Marc Vooijs ◽

Robert P. Coppes

Keyword(s):

Signaling Pathways ◽

Progenitor Cells ◽

Tissue Regeneration ◽

Normal Tissue ◽

Cellular Response ◽

Adult Tissue ◽

Tissue Specific ◽

Radiation Induced

Radiotherapy is involved in the treatment of many cancers, but damage induced to the surrounding normal tissue is often inevitable. Evidence suggests that the maintenance of homeostasis and regeneration of the normal tissue is driven by specific adult tissue stem/progenitor cells. These tasks involve the input from several signaling pathways. Irradiation also targets these stem/progenitor cells, triggering a cellular response aimed at achieving tissue regeneration. Here we discuss the currently used in vitro and in vivo models and the involved specific tissue stem/progenitor cell signaling pathways to study the response to irradiation. The combination of the use of complex in vitro models that offer high in vivo resemblance and lineage tracing models, which address organ complexity constitute potential tools for the study of the stem/progenitor cellular response post-irradiation. The Notch, Wnt, Hippo, Hedgehog, and autophagy signaling pathways have been found as crucial for driving stem/progenitor radiation-induced tissue regeneration. We review how these signaling pathways drive the response of solid tissue-specific stem/progenitor cells to radiotherapy and the used models to address this.

Download Full-text

Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum

International Journal of Implant Dentistry ◽

10.1186/s40729-021-00316-z ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Chu-nan Zhang ◽

Lin-yi Zhou ◽

Shu-jiao Qian ◽

Ying-xin Gu ◽

Jun-yu Shi ◽

...

Keyword(s):

Molecular Mechanisms ◽

Underlying Mechanism ◽

Cell Number ◽

Human Gingival Fibroblasts ◽

Superior Performance ◽

Control Group ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Gingival Fibroblasts ◽

Integrin Β1

Abstract Objectives This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms. Materials and methods Titanium plates were divided into two groups: (1) no coating (Ti, control), (2) Tantalum-coated titanium (Ta-coated Ti). All samples were characterized by scanning electronic microscopy, surface roughness, and hydrophilicity. Fibroblasts’ performance were analyzed by attached cell number at 1 h, 4 h, and 24 h, morphology at 1 h and 4 h, viability at 1 day, 3 days, 5 days, and 7 days, recovery after wounding at 6 h, 12 h, and 24 h. RT-PCR, western blot were applied to detect attachment-related genes’ expression and protein synthesis at 4 h and 24 h. Student’s t test was used for statistical analysis. Results Tantalum-coated titanium demonstrates a layer of homogeneously distributed nano-grains with mean diameter of 25.98 (± 14.75) nm. It was found that after tantalum deposition, human gingival fibroblasts (HGFs) adhesion, viability, proliferation, and migration were promoted in comparison to the control group. An upregulated level of Integrin β1 and FAK signaling was also detected, which might be the underlying mechanism. Conclusion In the present study, adhesion, viability, proliferation, migration of human gingival fibroblasts are promoted on tantalum-coated titanium, upregulated integrin β1 and FAK might contribute to its superior performance, indicating tantalum coating can be applied in transmucosal part of dental implant. Clinical significance Tantalum deposition on titanium surfaces can promote human gingival fibroblast adhesion, accordingly forming a well-organized soft tissue sealing and may contribute to a successful osseointegration.

Download Full-text