Newly Developed System for Acetamiprid Residue Screening in the Lettuce Samples Based on a Bioelectric Cell Biosensor

Population growth and increased production demands on fruit and vegetables have driven agricultural production to new heights. Nevertheless, agriculture remains one of the least optimized industries, with laboratory tests that take days to provide a clear result on the chemical level of produce. To address this problem, we developed a tailor-made solution for the industry that can allow multiple field tests on key pesticides, based on a bioelectric cell biosensor and the measurement of the cell membrane potential changes, according to the principle of the Bioelectric Recognition Assay (BERA). We developed a fully functional system that operates using a newly developed hardware for multiple data sources and an Android application to provide results within 3 min. The presence of acetamiprid residues caused a cell membrane hyperpolarization, which was distinguishable from the control samples. A database that classified samples Below or Above Maximum Residue Levels (MRL) was then created, based on a newly developed algorithm. Additionally, lettuce samples were analyzed with the conventional and the newly developed method, in parallel, revealing a high correlation on sample classification. Thus, it was demonstrated that the novel biosensor system could be used in the food supply chain to increase the number of tested products before they reach the market.

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Nitric oxide generated by the compound RuBPY promotes the vascular smooth cell membrane hyperpolarization

European Journal of Pharmaceutical Sciences ◽

10.1016/j.ejps.2013.01.003 ◽

2013 ◽

Vol 48 (4-5) ◽

pp. 604-610 ◽

Cited By ~ 6

Author(s):

Amanda C. Pereira ◽

Claure N. Lunardi ◽

Michele Paulo ◽

Roberto S. da Silva ◽

Lusiane M. Bendhack

Keyword(s):

Nitric Oxide ◽

Cell Membrane ◽

Membrane Hyperpolarization

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The GABAA agonist muscimol attenuates induced airway constriction in guinea pigs in vivo

Journal of Applied Physiology ◽

10.1152/japplphysiol.91314.2008 ◽

2009 ◽

Vol 106 (4) ◽

pp. 1257-1263 ◽

Cited By ~ 18

Author(s):

Neil R. Gleason ◽

George Gallos ◽

Yi Zhang ◽

Charles W. Emala

Keyword(s):

Smooth Muscle ◽

Cell Membrane ◽

Airway Smooth Muscle ◽

Guinea Pigs ◽

Neural Control ◽

Chronic Obstructive ◽

Airway Smooth Muscle Cells ◽

Membrane Hyperpolarization ◽

Airway Constriction

GABAA channels are ubiquitously expressed on neuronal cells and act via an inward chloride current to hyperpolarize the cell membrane of mature neurons. Expression and function of GABAA channels on airway smooth muscle cells has been demonstrated in vitro. Airway smooth muscle cell membrane hyperpolarization contributes to relaxation. We hypothesized that muscimol, a selective GABAA agonist, could act on endogenous GABAA channels expressed on airway smooth muscle to attenuate induced increases in airway pressures in anesthetized guinea pigs in vivo. In an effort to localize muscimol's effect to GABAA channels expressed on airway smooth muscle, we pretreated guinea pigs with a selective GABAA antagonist (gabazine) or eliminated lung neural control from central parasympathetic, sympathetic, and nonadrenergic, noncholinergic (NANC) nerves before muscimol treatment. Pretreatment with intravenous muscimol alone attenuated intravenous histamine-, intravenous acetylcholine-, or vagal nerve-stimulated increases in peak pulmonary inflation pressure. Pretreatment with the GABAA antagonist gabazine blocked muscimol's effect. After the elimination of neural input to airway tone by central parasympathetic nerves, peripheral sympathetic nerves, and NANC nerves, intravenous muscimol retained its ability to block intravenous acetylcholine-induced increases in peak pulmonary inflation pressures. These findings demonstrate that the GABAA agonist muscimol acting specifically via GABAA channel activation attenuates airway constriction independently of neural contributions. These findings suggest that therapeutics directed at the airway smooth muscle GABAA channel may be a novel therapy for airway constriction following airway irritation and possibly more broadly in diseases such as asthma and chronic obstructive pulmonary disease.

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Epidermal growth factor, like glucagon, exerts a short-term stimulation of alanine transport in rat hepatocytes

Biochemical Journal ◽

10.1042/bj2470233 ◽

1987 ◽

Vol 247 (1) ◽

pp. 233-235 ◽

Cited By ~ 28

Author(s):

S K Moule ◽

J D McGivan

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Cell Membrane ◽

Rat Hepatocytes ◽

Short Term ◽

Membrane Hyperpolarization ◽

Control Value ◽

Alanine Transport ◽

Epidermal Growth ◽

Stimulation Of

Epidermal growth factor causes a transient stimulation of alanine transport in hepatocytes. The stimulation is maximal after 30 min, and the rate returns to the control value after 90 min. These characteristics are very similar to the short-term stimulation of alanine transport by glucagon, which has been attributed to cell membrane hyperpolarization.

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Development of human cell biosensor system for genotoxicity detection based on DNA damage-induced gene expression

Radiology and Oncology ◽

10.2478/v10019-010-0010-3 ◽

2010 ◽

Vol 44 (1) ◽

Cited By ~ 13

Author(s):

Valerija Zager ◽

Maja Cemazar ◽

Irena Hreljac ◽

Tamara Lah ◽

Gregor Sersa ◽

...

Keyword(s):

Gene Expression ◽

Dna Damage ◽

Human Cell ◽

Biosensor System ◽

Cell Biosensor

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Development of a Fish Cell Biosensor System for Genotoxicity Detection Based on DNA Damage-Induced Trans-Activation of p21 Gene Expression

Biosensors ◽

10.3390/bios2030318 ◽

2012 ◽

Vol 2 (3) ◽

pp. 318-340 ◽

Cited By ~ 9

Author(s):

Deyu Geng ◽

Zhixia Zhang ◽

Huarong Guo

Keyword(s):

Gene Expression ◽

Dna Damage ◽

Fish Cell ◽

Biosensor System ◽

Cell Biosensor

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Differential membrane potential and ion current responses to different types of shear stress in vascular endothelial cells

AJP Cell Physiology ◽

10.1152/ajpcell.00243.2003 ◽

2004 ◽

Vol 286 (6) ◽

pp. C1367-C1375 ◽

Cited By ~ 47

Author(s):

Deborah K. Lieu ◽

Pamela A. Pappone ◽

Abdul I. Barakat

Keyword(s):

Endothelial Cells ◽

Shear Stress ◽

Membrane Potential ◽

Cell Membrane ◽

Oscillation Frequency ◽

Vascular Endothelial Cells ◽

Steady Shear ◽

Vascular Endothelial ◽

Membrane Hyperpolarization ◽

Different Types

Vascular endothelial cells (ECs) distinguish among and respond differently to different types of fluid mechanical shear stress. Elucidating the mechanisms governing this differential responsiveness is the key to understanding why early atherosclerotic lesions localize preferentially in arterial regions exposed to low and/or oscillatory flow. An early and very rapid endothelial response to flow is the activation of flow-sensitive K+ and Cl− channels that respectively hyperpolarize and depolarize the cell membrane and regulate several important endothelial responses to flow. We have used whole cell current- and voltage-clamp techniques to demonstrate that flow-sensitive hyperpolarizing and depolarizing currents respond differently to different types of shear stress in cultured bovine aortic ECs. A steady shear stress level of 10 dyn/cm2 activated both currents leading to rapid membrane hyperpolarization that was subsequently reversed to depolarization. In contrast, a steady shear stress of 1 dyn/cm2 only activated the hyperpolarizing current. A purely oscillatory shear stress of 0 ± 10 dyn/cm2 with an oscillation frequency of either 1 or 0.2 Hz activated the hyperpolarizing current but only minimally the depolarizing current, whereas a 5-Hz oscillation activated neither current. These results demonstrate for the first time that flow-activated ion currents exhibit different sensitivities to shear stress magnitude and oscillation frequency. We propose that flow-sensitive ion channels constitute components of an integrated mechanosensing system that, through the aggregate effect of ion channel activation on cell membrane potential, enables ECs to distinguish among different types of flow.

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Development of the Ammonia Biosensor Monitoring System

Water Science & Technology ◽

10.2166/wst.1993.0683 ◽

1993 ◽

Vol 28 (11-12) ◽

pp. 435-445 ◽

Cited By ~ 3

Author(s):

H. Tanaka ◽

E. Nakamura ◽

H. Hoshikawa ◽

Y. Tanaka

Keyword(s):

Wastewater Treatment ◽

Wastewater Treatment Plant ◽

Treatment Plant ◽

Phosphate Buffer Solution ◽

Field Tests ◽

Nitrifying Bacteria ◽

Secondary Effluent ◽

Buffer Solution ◽

Biosensor System ◽

Isothermal Water

The ammonia biosensor system was newly developed for monitoring ammonia in a river and in secondary effluent from a wastewater treatment plant. The detector of the ammonia biosensor is nitrifying bacteria immobilized by two acetic cellulose membranes and the transducer of the sensor is a dissolved oxygen probe. The microbial probe performs excellent selectivity and quick response to ammonia although control of pH with a phosphate buffer solution and control of temperature with an isothermal water bath are required. The developed ammonia biosensor system processes automatic calibration, sampling, and measurement as a monitor in the field. Accuracy and reliability of the biosensor system were also demonstrated by field tests accomplished at a wastewater treatment plant and at a river quality monitoring station.

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Effects of Prolactin on Ionic Membrane Conductances in the Human Malignant Astrocytoma Cell Line U87-MG

Journal of Neurophysiology ◽

10.1152/jn.00710.2003 ◽

2004 ◽

Vol 91 (3) ◽

pp. 1203-1216 ◽

Cited By ~ 5

Author(s):

Thomas Ducret ◽

Anne-Marie Vacher ◽

Pierre Vacher

Keyword(s):

Cell Membrane ◽

Cell Line ◽

Malignant Astrocytoma ◽

Patch Clamp Technique ◽

Peripheral Tissues ◽

Membrane Hyperpolarization ◽

Membrane Conductances ◽

Astrocytoma Cell Line ◽

Astrocytoma Cell ◽

Ion Conductances

Prolactin (PRL) is involved in numerous biological processes in peripheral tissues and the brain. Although numerous studies have been conducted to elucidate the signal transduction pathways associated with the PRL receptor, very few have examined the role of ion conductances in PRL actions. We used the patch-clamp technique in “whole cell” configuration and microspectrofluorimetry to investigate the effects of PRL on membrane ion conductances in the U87-MG human malignant astrocytoma cell line, which naturally expresses the PRL receptor. We found that a physiological concentration (4 nM) of PRL exerted a biphasic action on membrane conductances. First, PRL activated a Ca2+-dependent K+ current that was sensitive to CTX and TEA. This current depended on PRL-induced Ca2+ mobilization, through a JAK2-dependent pathway from a thapsigargin- and 2-APB-sensitive Ca2+ pool. Second, PRL also activated an inwardly directed current, mainly due to the stimulation of calcium influx via nickel- and 2-APB-sensitive calcium channels. Both phases resulted in membrane hyperpolarizations, mainly through the activation of Ca2+-dependent K+ channels. As shown by combined experiments (electrophysiology and microspectrofluorimetry), the PRL-induced Ca2+ influx increased with cell membrane hyperpolarization and conversely decreased with cell membrane depolarization. Thus PRL-induced membrane hyperpolarizations facilitated Ca2+ influx through voltage-independent Ca2+ channels. Finally, PRL also activated a DIDS-sensitive Cl- current, which may participate in the PRL-induced hyperpolarization. These PRL-induced conductance activations are probably related to the PRL proliferative effect we have already described in U87-MG cells.

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