scholarly journals Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3684 ◽  
Author(s):  
Yesong Gu ◽  
Po-Yuan Tseng ◽  
Xiang Bi ◽  
Jason Yang
Keyword(s):  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Complementary Sequence ◽  
Signal Decay ◽  
Target Dna ◽  
Hybridization Efficiency ◽  
Sh Group ◽  
Polymerase Chain ◽  
Or Gene

The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming. In this study, we have proposed a facile strategy to construct a DNA biosensor, in which the platinum electrode was modified with a dual-film of electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) resulting in immobilized gold nanoparticles, with the gold nanoparticles easily immobilized in a uniform distribution. The DNA probe labeled with a SH group was then assembled to the fabricated electrode and employed to capture the target DNA based on the complementary sequence. The hybridization efficiency was evaluated with differential pulse voltammetry (DPV) in the presence of daunorubicin hydrochloride. Our results demonstrated that the peak current in DPV exhibited a linear correlation the concentration of target DNA that was complementary to the probe DNA. Moreover, the electrode could be reused by heating denaturation and re-hybridization, which only brought slight signal decay. In addition, the addition of the oxidized form of nicotinamide adenine dinucleotide (NAD+) could dramatically enhance the sensitivity by more than 5.45-fold, and the limit-of-detection reached about 100 pM.

scholarly journals Sensitive and Selective Electrochemical Detection of Epirubicin as Anticancer Drug Based on Nickel Ferrite Decorated with Gold Nanoparticles

Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1334
Author(s):  
Mohammad Mehmandoust ◽  
Nevin Erk ◽  
Ceren Karaman ◽  
Fatemeh Karimi ◽  
Sadegh Salmanpour
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Nickel Ferrite ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
X Ray ◽  
Transmission Electron ◽  
The Stability ◽  
Electrochemical Electrode

The accurate and precise monitoring of epirubicin (EPR), one of the most widely used anticancer drugs, is significant for human and environmental health. In this context, we developed a highly sensitive electrochemical electrode for EPR detection based on nickel ferrite decorated with gold nanoparticles (Au@NiFe2O4) on the screen-printed electrode (SPE). Various spectral characteristic methods such as Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-Vis), energy-dispersive X-ray spectroscopy (EDX) and electrochemical impedance spectroscopy (EIS) were used to investigate the surface morphology and structure of the synthesized Au@NiFe2O4 nanocomposite. The novel decorated electrode exhibited a high electrocatalytic activity toward the electrooxidation of EPR, and a nanomolar limit of detection (5.3 nM) was estimated using differential pulse voltammetry (DPV) with linear concentration ranges from 0.01 to 0.7 and 0.7 to 3.6 µM. The stability, selectivity, repeatability reproducibility and reusability, with a very low electrode response detection limit, make it very appropriate for determining trace amounts of EPR in pharmaceutical and clinical preparations.

scholarly journals Novel Competitive Voltammetric Aptasensor Based on Electrospun Carbon Nanofibers-Gold Nanoparticles Modified Graphite Electrode for Salmonella enterica serovar Detection

2020 ◽  
Vol 11 (2) ◽  
pp. 8702-8715
Keyword(s):  
Gold Nanoparticles ◽  
Carbon Nanofibers ◽  
Electrochemical Detection ◽  
Salmonella Enterica ◽  
Electrochemical Impedance ◽  
Graphite Electrode ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Pencil Graphite Electrode ◽  
Experimental Conditions

Salmonella enterica is considered one of the most common bacterial agent causes of acute gastroenteritis and foodborne illness in humans worldwide. Antibiotic-resistant is considered as a major problem in Salmonella enterica Serovar. This study introduces a new simple and sensitive aptasensor based on chitosan (Chi)-electrospun carbon nanofibers (CNF) /gold nanoparticles (GNPs) decorated pencil graphite electrode (GE) as a novel platform for electrochemical detection of Salmonella enterica Serovar. A Salmonella-specific recognition aptamer ssDNA sequence was used in the development of this voltammetric biosensor. Electrochemical behaviors of electrodes; unmodified GE, CNF-Chi/GE, GNPs/CNF-Chi/GE, GNPs/CNF-Chi/GEs linked with the aptamer were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). After the optimization of experimental conditions (e.g., CNF concentration, pH, and incubation time), electrochemical detection of Salmonella was performed via differential pulse voltammetry (DPV) in methylene blue solution. The designed aptasensor exhibited a linear range of 10 to 105 (CFU/mL) with the limit of detection (LOD) 1.223 (Cfu/mL) for Salmonella. This aptasensor displayed excellent selectivity and remarkable sensitivity in terms of the detection of Salmonella enterica even in the real samples as compared to the polymerase chain reaction (PCR) technique. The constructed aptasensor is a highly sensitive sensor for the detection of Salmonella enterica and also can be tailored for various other targets.

scholarly journals A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Xin Li ◽  
Tao Song ◽  
Zhihua Chen ◽  
Xiaolong Shi ◽  
Congzhou Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Signal Detection ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Strand Displacement ◽  
Target Dna ◽  
Dna Strand Displacement ◽  
Or Gene ◽  
Dna Strand ◽  
Visual Results

DNA or gene signal detection is of great significance in many fields including medical examination, intracellular molecular monitoring, and gene disease signal diagnosis, but detection of DNA or gene signals in a low concentration with instant visual results remains a challenge. In this work, a universal fast and visual colorimetric detection method for DNA signals is proposed. Specifically, a DNA signal amplification “circuit” based on DNA strand displacement is firstly designed to amplify the target DNA signals, and then thiol modified hairpin DNA strands and gold nanoparticles are used to make signal detection results visualized in a colorimetric manner. If the target DNA signal exists, the gold nanoparticles aggregate and settle down with color changing from dark red to grey quickly; otherwise, the gold nanoparticles’ colloids remain stable in dark red. The proposed method provides a novel way to detect quickly DNA or gene signals in low concentrations with instant visual results. When applied in real-life, it may provide a universal colorimetric method for gene disease signal diagnosis.

A Porphyrin Molecularly Imprinted Biomimetic Electrochemical Sensor Based on Gold Nanoparticles and Carboxyl Graphene Composite for the Highly Efficient Detection of Methyl Parathion

NANO ◽  
2017 ◽  
Vol 12 (04) ◽  
pp. 1750046 ◽  
Author(s):  
Bin He ◽  
Ya-Li Mao ◽  
Ya Zhang ◽  
Wei Yin ◽  
Chang-Jun Hou ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Methyl Parathion ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Molecularly Imprinted ◽  
Zinc Porphyrin ◽  
Efficient Detection ◽  
Novel Strategy ◽  
Biomimetic Sensor

A highly sensitive and selective biomimetic sensor based on zinc porphyrin molecularly imprinted Polymer microspheres (MIPMs), gold nanoparticles (AuNPs) and carboxyl graphene (CG) nanomaterials was successfully developed for direct electrochemical detection of methyl parathion (MP). The novel strategy emphasized the fabrication of a porphyrin zinc-based sensor via attaching MIPMs on AuNPs/CG nanocomposites. MIPMs was prepared by free radical polymerization using MP as the template, Zinc porphyrin as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking reagent and azobisisobutyronitrile (AIBN) as the initiator. The introduction of AuNPs/CG significantly increased the effective electrode area, and amplified the sensor signal. The modified electrode was characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The parameters of the detection process were also optimized. The biomimetic sensor exhibits a much wider linear dynamic range between 1.0[Formula: see text][Formula: see text][Formula: see text]10[Formula: see text][Formula: see text]mol L[Formula: see text] and 8.0[Formula: see text][Formula: see text][Formula: see text]10[Formula: see text][Formula: see text]mol L[Formula: see text] and the limit of detection (LOD) down to 3.16[Formula: see text][Formula: see text][Formula: see text]10[Formula: see text][Formula: see text]mol L[Formula: see text] based on S/N [Formula: see text] 3. The sensor had good reproducibility, stability and selectivity for MP detection. The developed sensor was successfully employed for the detection of MP in real samples.

Gold Nanoparticle Based Electrochemical Immunosensor for Detection of T3 Hormone

2020 ◽  
Vol 20 (10) ◽  
pp. 6057-6062
Author(s):  
Rahul Saxena ◽  
H. Fouad ◽  
Sudha Srivastava
Keyword(s):  
Gold Nanoparticles ◽  
Cyclic Voltammetry ◽  
Electrode Surface ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Electrochemical Immunosensor ◽  
Antigen Concentration ◽  
Antigen Present ◽  
Pulse Voltammetry

We report a nanoparticles based electrochemical immunosensor to detect and quantify triiodothyronine (T3) hormone. Immunosensor developed using gold nanoparticles and anti-T3 antibody, was employed for quantification of T3 antigen using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) technique. The electrochemical response of the developed immunosensor correlates well with the amount of antigen present in the sample. With increase in antigen concentration the immunocomplex formation on electrode surface increases and hence redox current decreases. The immunosensor shows a lower limit of detection of 1 pg/mL and dynamic range from 1 to 500 pg/mL. Sensitivity of the immunosensor was found to be 29.81 μA/pg/mL/cm2.

scholarly journals Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles

Biosensors ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 78
Author(s):  
Dezhi Feng ◽  
Jing Su ◽  
Guifang He ◽  
Yi Xu ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Early Diagnosis ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Target Dna ◽  
Potential Applications ◽  
Sandwich System

BRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TSP) and poly-adenine (polyA) mediated gold nanoparticles (AuNPs) for the sensitive detection of BRCA1. A thiol-modified TSP was used as the scaffold on the surface of the screen-printed AuNPs electrode. The capture DNA (TSP) and reporter DNA were hybridized to the target DNA (BRCA1), respectively, to form the typical sandwich system. The nanocomposites of reporter DNA (polyA at the 5′ end) combined with AuNPs were employed for signal amplification which can capture multiple enzymes by the specificity between biotin and streptavidin. Measurements were completed in the electrochemical workstation by cyclic voltammetry and amperometry and we obtained the low limit of detection of 0.1 fM with the linear range from 1 fM to 1 nM. High sensitivity and good specificity of the proposed electrochemical DNA sensor showed potential applications in clinical early diagnosis for breast cancer.

scholarly journals Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Yuzhong Zhang ◽  
Zhen Wang ◽  
Yuehong Wang ◽  
Lei Huang ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Dna Biosensor ◽  
Mercaptoacetic Acid ◽  
Electrochemical Impedance Spectra ◽  
Target Dna ◽  
Covalently Linked ◽  
Film Assembly

A sensitive electrochemical DNA biosensor was prepared based on mercaptoacetic acid (MAA)/gold nanoparticles (AuNPs) modified electrode. Probe DNA (NH2-DNA) was covalently linked to the carboxyl group of MAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxyl-succinimide (NHS). Scanning electron microscopy (SEM) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. The DNA hybridization events were monitored by differential pulse voltammetry (DPV), and adriamycin was used as the electrochemical indicator. Also the factors influencing the performance of the DNA hybridization were investigated in detail. Under the optimal conditions, the signal was linearly changed with target DNA concentration increased from 5.0 × 10−13to 1.0 × 10−9 M and had a detection limit of 1.7 × 10−13 M (signal/noise ratio of 3). In addition, the DNA biosensor showed good reproducibility and stability during DNA assay.

scholarly journals Interferences of PCR effectivity: importance for quantitative analyse

2010 ◽  
Vol 27 (Special Issue 2) ◽  
pp. 42-49 ◽  
Author(s):  
J. Hodek ◽  
J. Ovesná ◽  
L. Kučera
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Genetically Modified Organisms ◽  
Dna Quantification ◽  
Chain Reaction ◽  
Quantitative Analyse ◽  
Pcr Efficiency ◽  
Target Dna ◽  
Polymerase Chain ◽  
Or Gene

Importance of the Polymerase chain reaction (PCR) have already crossed the border of mere target DNA sequence present or absence analysis. For number analyses e.g. Genetically Modified Organisms (GMOs) or gene expression assesment the DNA quantification is demanded. Real-time (or quantitative) PCR is the most used tool for nucleic acids quantification. PCR efficiency has relevant importance on DNA quantification – it should be almost same for each PCR and its value should varied between 90–100%. There are a lot of PCR enhancers and inhibitors well known. We described impact of used DNA solvent and used laboratory plastic on real-time PCR efficiency.

Indirect Determination of Amikacin by Gold Nanoparticles as Redox Probe

2020 ◽  
Vol 17 ◽  
Author(s):  
Mansureh Alizadeh ◽  
Mandana Amiri ◽  
Abolfazl Bezaatpour
Keyword(s):  
Gold Nanoparticles ◽  
Bacterial Infections ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Cathodic Peak ◽  
Easy Method ◽  
Peak Current ◽  
Indirect Determination ◽  
Tract Infections

: Amikacin is an aminoglycoside antibiotic used for many gram-negative bacterial infections like infections in the urinary tract, infections in brain, lungs and abdomen. Electrochemical determination of amikacin is a challenge in electroanalysis because it shows no voltammetric peak at the surface of bare electrodes. In this approach, a very simple and easy method for indirect voltammetric determination of amikacin presented in real samples. Gold nanoparticles were electrodeposited at the surface of glassy carbon electrode in constant potential. The effect of several parameters such as time and potential of deposition, pH and scan rates on signal were studied. The cathodic peak current of Au3+ decreased with increasing amikacin concentration. Quantitative analysis of amikacin was performed using differential pulse voltammetry by following cathodic peak current of gold ions. Two dynamic linear ranges of 1.0 × 10−8–1.0 × 10-7 M and 5.0 × 10−7–1.0 × 10-3 M were obtained and limit of detection was estimated 3.0× 10−9 M. The method was successfully determined amikacin in pharmaceutical preparation and human serum. The effect of several interference in determination of amikacin was also studied.

scholarly journals Simultaneous electrochemical sensing of dihydroxy benzene isomers at cost-effective allura red polymeric film modified glassy carbon electrode

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pattan-Siddappa Ganesh ◽  
Ganesh Shimoga ◽  
Seok-Han Lee ◽  
Sang-Youn Kim ◽  
Eno E. Ebenso
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Oxidation Potential ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Allura Red

Abstract Background A simple and simultaneous electrochemical sensing platform was fabricated by electropolymerization of allura red on glassy carbon electrode (GCE) for the interference-free detection of dihydroxy benzene isomers. Methods The modified working electrode was characterized by electrochemical and field emission scanning electron microscopy methods. The modified electrode showed excellent electrocatalytic activity for the electrooxidation of catechol (CC) and hydroquinone (HQ) at physiological pH of 7.4 by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techniques. Results The effective split in the overlapped oxidation signal of CC and HQ was achieved in a binary mixture with peak to peak separation of 0.102 V and 0.103 V by CV and DPV techniques. The electrode kinetics was found to be adsorption-controlled. The oxidation potential directly depends on the pH of the buffer solution, and it witnessed the transfer of equal number of protons and electrons in the redox phenomenon. Conclusions The limit of detection (LOD) for CC and HQ was calculated to be 0.126 μM and 0.132 μM in the linear range of 0 to 80.0 μM and 0 to 110.0 μM, respectively, by ultra-sensitive DPV technique. The practical applicability of the proposed sensor was evaluated for tap water sample analysis, and good recovery rates were observed. Graphical abstract Electrocatalytic interaction of ALR/GCE with dihydroxy benzene isomers.

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