scholarly journals Trends in the Design of Intensity-Based Optical Fiber Biosensors (2010–2020)

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 197
Author(s):  
Nerea De Acha ◽  
Abián B. Socorro-Leránoz ◽  
César Elosúa ◽  
Ignacio R. Matías
Keyword(s):  
Optical Fiber ◽  
Limit Of Detection ◽  
Early Stage ◽  
Point Of Care ◽  
Research Groups ◽  
Fiber Sensors ◽  
Environment Quality ◽  
Low Concentrations ◽  
Highly Sensitive ◽  
Fiber Dimensions

There exists an increasing interest in monitoring low concentrations of biochemical species, as they allow the early-stage detection of illnesses or the monitoring of the environment quality. Thus, both companies and research groups are focused on the development of accurate, fast and highly sensitive biosensors. Optical fiber sensors have been widely employed for these purposes because they provide several advantages for their use in point-of-care and real-time applications. In particular, this review is focused on optical fiber biosensors based on luminescence and absorption. Apart from the key parameters that determine the performance of a sensor (limit of detection, sensibility, cross-sensibility, etc.), other features are analyzed, such as the optical fiber dimensions, the sensing set ups and the fiber functionalization. The aim of this review is to have a comprehensive insight of the different aspects that must be taken into account when working with this kind of sensors.

scholarly journals Optical Fiber Gratings Immunoassays

Sensors ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 2595 ◽  
Author(s):  
Médéric Loyez ◽  
Maxime Lobry ◽  
Ruddy Wattiez ◽  
Christophe Caucheteur
Keyword(s):  
Optical Fibers ◽  
Limit Of Detection ◽  
Ex Vivo ◽  
Point Of Care ◽  
Label Free ◽  
Intrinsic Properties ◽  
Fiber Gratings ◽  
The Core ◽  
Low Concentrations ◽  
Highly Sensitive

Optical fibers are of growing interest for biosensing, especially for point-of-care and biomedical assays. Their intrinsic properties bestow them sought-after assets for the detection of low concentrations of analytes. Tilted fiber Bragg gratings (TFBGs) photo-inscribed in the core of telecommunication-grade optical fibers are known to be highly-sensitive refractometers. In this work, we present different strategies to use them for label-free immunoassays. Bare, gold-sputtered, gold-electroless-plated (ELP) and hybrid configurations are biofunctionalized with antibodies, aiming at the detection of cancer biomarkers. We discuss the relative performances of the tested configurations and show that each leads to singular key features, which therefore drives their selection as a function of the target application. The most sensitive configuration presents a limit of detection of 10−12 g/mL in laboratory settings and was successfully used ex vivo in freshly resected lung tissues.

scholarly journals Highly Sensitive Extrinsic X-Ray Polymer Optical Fiber Sensors Based on Fiber Tip Modification

2017 ◽  
Vol 17 (16) ◽  
pp. 5112-5117 ◽  
Author(s):  
Ana I. de Andres ◽  
Sinead O'Keeffe ◽  
Lingxia Chen ◽  
Oscar Esteban
Keyword(s):  
Optical Fiber ◽  
Optical Fiber Sensors ◽  
Polymer Optical Fiber ◽  
Fiber Sensors ◽  
X Ray ◽  
Highly Sensitive

scholarly journals Analytical validation of a highly sensitive point-of-care system for cardiac troponin I determination

2019 ◽  
Vol 58 (1) ◽  
pp. 138-145 ◽  
Author(s):  
Federica Braga ◽  
Elena Aloisio ◽  
Andrea Panzeri ◽  
Takahito Nakagawa ◽  
Mauro Panteghini
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Limit Of Detection ◽  
Clinical Performance ◽  
Point Of Care ◽  
Total Error ◽  
Method Comparison ◽  
Reference Limit ◽  
Highly Sensitive ◽  
Limit Of Quantitation

Abstract Background Highly sensitive cardiac troponin assays (hs-cTn) are not available as point-of-care (POC) measurements. As rapid testing cannot be achieved at the expense of clinical performance, there is an urgent need to develop and rigorously validate POC hs-cTn. Konica Minolta (KM) has recently developed a surface plasmon-field enhanced fluorescence spectroscopy-based POC hs-cTn I system. Methods We validated the analytical characteristics of the KM POC system according to the international guidelines. Results Limit of blank (LoB) and limit of detection (LoD) were 0.35 and 0.62 ng/L, respectively, hs-cTn I concentrations corresponding to a total CV of 20%, 10% and 5% were 1.5, 3.9 and 11.0 ng/L, respectively. Method comparison studies showed that KM calibration was successfully traced to higher-order references. Limit of quantitation (LoQ), i.e. the hs-cTn I concentration having a total error of measurement of ≤34%, was 10.0 ng/L. The upper reference limit (URL) for 600 healthy blood donors was calculated at 12.2 ng/L (90% confidence interval [CI]: 9.2–39.2), while sex-partitioned URLs were 20.6 (males) and 10.7 ng/L (females), respectively (p < 0.0001). KM assay measured hs-cTn I concentrations >LoD in 65.7% of all reference individuals, in 76.7% of males and in 54.7% of females, respectively. Conclusions The KM system joins the characteristics of POC systems to the analytical performance of hs-cTn.

scholarly journals Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4902
Author(s):  
Anna Go ◽  
Sung Ryul Park ◽  
Yejin Ku ◽  
Mingge Sun ◽  
Sangho Yeon ◽  
...  
Keyword(s):  
Ketone Bodies ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Modified Electrodes ◽  
Electrochemical Measurement ◽  
Clinical Results ◽  
Current Increase ◽  
Sensing Material ◽  
Highly Sensitive ◽  
Good Agreement

In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-β-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing material to investigate its electroanalytical properties by means of cyclic voltammetry (CV). The modified electrodes are used for the detection of AcAc and present a linear current increase when the AcAc concentration increases. The electrode presents a limit of detection (LOD) of 6.25 mg/dL in the range of 6.25–100 mg/dL for investigation of clinical relevance. Finally, the electrode was evaluated using 20 patient samples. The measured results of urine ketone by the developed electrode were compared with the clinical results from a commercial kit, and the analysis showed good agreement. The proposed electrode was demonstrated to be a very promising platform as a miniaturized electrochemical analyzer for point-of-care monitoring of the critical biochemical parameters such as urine ketone.

scholarly journals Specificity of Biomarker Detection in Microfluidic Sensors

2020 ◽  
Author(s):  
Praneet Prakash ◽  
Manoj Varma
Keyword(s):  
Early Stage ◽  
Point Of Care ◽  
Biomarker Detection ◽  
Reaction Parameter ◽  
Reaction Parameters ◽  
Specific Detection ◽  
Early Detection Of Disease ◽  
Low Concentrations ◽  
Microfluidic Sensors ◽  
Weak Target

<p>The microfluidics based point-of-care (POC) sensing devices offer unmatched possibilities of fast and high throughput diagnosis over conventional strategies. A major challenge for the early detection of disease is the significantly lower concentration of biomarkers as compared to the interfering noise molecules. In this work, we investigate the ‘reaction parameter’ phase space to identify suitable reaction parameters to enhance biomarker detection specificity. Under similar target biomarker and noise concentration levels, we show that a target biomarker is more likely to be detected at low concentrations and weak target and noise-receptor binding kinetics. Importantly, a simulation verified time-scale based methodology is developed to guide the appropriate choice of biomarkers for specific detection. This study demonstrates the prospect of successful POC diagnostic devices during early stage of diseases such as cancer. </p>

scholarly journals Specificity of Biomarker Detection in Microfluidic Sensors

2020 ◽  
Author(s):  
Praneet Prakash ◽  
Manoj Varma
Keyword(s):  
Early Stage ◽  
Point Of Care ◽  
Biomarker Detection ◽  
Reaction Parameter ◽  
Reaction Parameters ◽  
Specific Detection ◽  
Early Detection Of Disease ◽  
Low Concentrations ◽  
Microfluidic Sensors ◽  
Weak Target

<p>The microfluidics based point-of-care (POC) sensing devices offer unmatched possibilities of fast and high throughput diagnosis over conventional strategies. A major challenge for the early detection of disease is the significantly lower concentration of biomarkers as compared to the interfering noise molecules. In this work, we investigate the ‘reaction parameter’ phase space to identify suitable reaction parameters to enhance biomarker detection specificity. Under similar target biomarker and noise concentration levels, we show that a target biomarker is more likely to be detected at low concentrations and weak target and noise-receptor binding kinetics. Importantly, a simulation verified time-scale based methodology is developed to guide the appropriate choice of biomarkers for specific detection. This study demonstrates the prospect of successful POC diagnostic devices during early stage of diseases such as cancer. </p>

scholarly journals Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction

2020 ◽  
Author(s):  
Shan Wei ◽  
Esther Kohl ◽  
Alexandre Djandji ◽  
Stephanie Morgan ◽  
Susan Whittier ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Diagnostic Testing ◽  
Rna Extraction ◽  
Nucleic Acid Amplification ◽  
Nasopharyngeal Swab ◽  
Viral Transport ◽  
Extraction Step ◽  
Highly Sensitive

AbstractThe COVID-19 pandemic has resulted in an urgent global need for rapid, point-of-care diagnostic testing. Existing methods for nucleic acid amplification testing (NAAT) require an RNA extraction step prior to amplification of the viral RNA. This step necessitates the use of a centralized laboratory or complex and costly proprietary cartridges and equipment, and thereby prevents low-cost, scalable, point-of-care testing. We report the development of a highly sensitive and robust, easy-to-implement, SARS-CoV-2 test that utilizes isothermal amplification and can be run directly on viral transport media following a nasopharyngeal swab without the need for prior RNA extraction. Our assay provides visual results in 30 min with 85% sensitivity, 100% specificity, and a limit of detection (LoD) of 2.5 copies/μl, and can be run using a simple heat block.

scholarly journals Highly Sensitive Refractive Index Optical Fiber Sensors Fabricated by a Femtosecond Laser

2011 ◽  
Vol 3 (6) ◽  
pp. 1189-1197 ◽  
Author(s):  
Jinpeng Yang ◽  
Lan Jiang ◽  
Sumei Wang ◽  
Qianghua Chen ◽  
Benye Li ◽  
...  
Keyword(s):  
Refractive Index ◽  
Femtosecond Laser ◽  
Optical Fiber ◽  
Optical Fiber Sensors ◽  
Fiber Sensors ◽  
Highly Sensitive

Ultra-Sensitive Point of Care Biosensor for Detecting Pathogeneses

2019 ◽  
Vol 28 (03n04) ◽  
pp. 1940014
Author(s):  
Negin Farzad ◽  
Chengde Cui ◽  
Ali Senejani ◽  
Saion Sinha
Keyword(s):  
Viral Infections ◽  
Early Stage ◽  
Point Of Care ◽  
High Specificity ◽  
Viral Disease ◽  
Diagnostic Techniques ◽  
Effective Manner ◽  
Highly Sensitive ◽  
Selective Basis ◽  
Pcr Techniques

Pathogen diseases cause considerable loses in production of food which impact human health from diverse bacterial/viral infections. Precise spotting and diagnosis of such infectious disease is significant to prevent it from further outbreak issues. Moreover, to detect this kind of diseases at an early stage with highly sensitive and selective basis is necessary to avoid the spread of invasive pathogens. The conventional methods such as ELISA, PCR techniques are currently in use to diagnose bacterial/viral disease with high throughput. Though these diagnostic techniques assist in detect and identify the diseases, there are few modern challenges to be meet in order to make this diagnostic more effective in recent days. In this paper, our designed device consists of Bionanosensor works on nucleic acid-based testing provides result with high specificity and selectivity which is vital for early stage identification in a rapid real-time effective manner

scholarly journals Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1556
Author(s):  
Melike Karakaya
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Extraction Process ◽  
Single Step ◽  
Enzymatic Amplification ◽  
Whole Process ◽  
Low Concentrations ◽  
Paper Microfluidic

In this work, we focused on the development of a novel point of care (POC) paper-based analytical microfluidic chip. The system has been developed for paper-based extraction, non-enzymatic amplification and electroanalytical detection of human papillomavirus (HPV). The device comprises paper extraction support material as well as paper-based detection tool. Herein, a special DNA modification method has been utilized to allow non-enzymatic amplification by using microbeads and silver nanoparticles labeled primers. The device is capable of extracting more than 10–100 copies per mL of DNA approximately in 15 min along with a single step extraction process from patient samples. In addition, it is able to detect low concentrations taking just less than 10 min with high selectivity to HPV kinds of 16&18. It only takes the low-cost point of care device less than 40 min with a low limit of detection (LOD) to complete the whole process.

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