scholarly journals Effects of Aerobic Exercise Training on MyomiRs Expression in Cachectic and Non-Cachectic Cancer Mice

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5728
Author(s):  
João Lucas Penteado Gomes ◽  
Gabriel Cardial Tobias ◽  
Tiago Fernandes ◽  
André Casanova Silveira ◽  
Carlos Eduardo Negrão ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Skeletal Muscle ◽  
Colon Cancer ◽  
Tibialis Anterior Muscle ◽  
Aerobic Exercise Training ◽  
Cross Sectional ◽  
Disease Evolution ◽  
Serum Microrna ◽  
Potential Biomarker ◽  
Mtor Protein

We investigated the effects of AET on myomiRs expression in the skeletal muscle and serum of colon cachectic (CT26) and breast non-cachectic (MMTV-PyMT) cancer mice models. Colon cancer decreased microRNA-486 expression, increasing PTEN in tibialis anterior muscle (TA), decreasing the PI3K/mTOR protein pathway, body and muscle wasting, fibers’ cross-sectional area and muscle dysfunction, that were not preserved by AET. In contrast, breast cancer decreased those muscle functions, but were preserved by AET. In circulation, the downregulation of microRNA-486 and -206 in colon cancer, and the downregulation of microRNA-486 and upregulation of microRNA-206 expression in breast cancer might be good cancer serum biomarkers. Since the microRNA-206 is skeletal muscle specific, their expression was increased in the TA, serum and tumor in MMTV, suggesting a communication among these three compartments. The AET prevents these effects on microRNA-206, but not on microRNA-486 in MMTV. In conclusion, cancer induced a downregulation of microRNA-486 expression in TA and serum of CT26 and MMTV mice and these effects were not prevented by AET; however, to MMTV, the trained muscle function was preserved, probably sustained by the downregulation of microRNA-206 expression. Serum microRNA-206 is a potential biomarker for colon (decreased) and breast (increased) cancer to monitor the disease evolution and the effects promoted by the AET.

Serum microRNA as potential biomarker to detect breast atypical hyperplasia and early-stage breast cancer

2018 ◽  
Vol 14 (30) ◽  
pp. 3145-3161 ◽  
Author(s):  
Xuefeng An ◽  
Hong Quan ◽  
Jinhui Lv ◽  
Lingyu Meng ◽  
Cheng Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Stage ◽  
Atypical Hyperplasia ◽  
Early Stage Breast Cancer ◽  
Serum Microrna ◽  
Potential Biomarker

scholarly journals Serum microRNA-155 as a potential biomarker for breast cancer screening

2012 ◽  
Vol 57 (26) ◽  
pp. 3466-3468 ◽  
Author(s):  
SuYing Zhao ◽  
Qian Wu ◽  
Fen Gao ◽  
ChunBing Zhang ◽  
XueWen Yang
Keyword(s):  
Breast Cancer ◽  
Cancer Screening ◽  
Breast Cancer Screening ◽  
Serum Microrna ◽  
Potential Biomarker

scholarly journals Serum MicroRNA-155 as a Potential Biomarker to Track Disease in Breast Cancer

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47003 ◽  
Author(s):  
Yu Sun ◽  
Minjie Wang ◽  
Guigao Lin ◽  
Shipeng Sun ◽  
Xuexiang Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Serum Microrna ◽  
Potential Biomarker

scholarly journals The Maintenance of Muscle Mass Is Independent of Testosterone in Adult Male Mice

2020 ◽  
Author(s):  
Arik Davidyan ◽  
Keith Baar ◽  
Sue C. Bodine
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Muscle Mass ◽  
Tibialis Anterior ◽  
Skeletal Muscle Mass ◽  
Tibialis Anterior Muscle ◽  
Male Mice ◽  
Cross Sectional ◽  
Western Blots ◽  
Physiological Serum

AbstractTestosterone is considered a potent anabolic agent in skeletal muscle with a well-established role in adolescent growth and development in males. However, alterations in the role of testosterone in the regulation of skeletal muscle mass and function throughout the lifespan has yet to be established. While some studies suggest that testosterone is important for the maintenance of skeletal muscle mass, an understanding of the role this hormone plays in young, adult, and old males with normal and low serum testosterone levels is lacking. We investigated the role testosterone plays in the maintenance of muscle mass by examining the effect of orchiectomy-induced testosterone depletion in C57Bl6 male mice at ages ranging from early postnatal through old age; the age groups we used included 1.5-, 5-, 12-, and 24-month old mice. Following 28 days of testosterone depletion, we assessed mass and fiber cross-sectional-area (CSA) of the tibialis anterior, gastrocnemius, and quadriceps muscles. In addition, we measured global rates of protein synthesis and degradation using the SuNSET method, western blots, and enzyme activity assays. 28 days of testosterone depletion resulted in smaller muscle mass in the two youngest cohorts but had no effect in the two older ones. Mean CSA decreased only in the youngest cohort and only in the tibialis anterior muscle. Testosterone depletion resulted in a general increase in proteasome activity at all ages. We did not detect changes in protein synthesis at the terminal time point. This data suggest that within physiological serum concentrations, testosterone is not important for the maintenance of muscle mass in mature male mice; however, in young mice testosterone is crucial for normal growth.

scholarly journals Maintenance of muscle mass in adult male mice is independent of testosterone

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0240278
Author(s):  
Arik Davidyan ◽  
Suraj Pathak ◽  
Keith Baar ◽  
Sue C. Bodine
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Muscle Mass ◽  
Tibialis Anterior ◽  
Skeletal Muscle Mass ◽  
Tibialis Anterior Muscle ◽  
Male Mice ◽  
Cross Sectional ◽  
Western Blots ◽  
Physiological Serum

Testosterone is considered a potent anabolic agent in skeletal muscle with a well-established role in adolescent growth and development in males. However, the role of testosterone in the regulation of skeletal muscle mass and function throughout the lifespan has yet to be fully established. While some studies suggest that testosterone is important for the maintenance of skeletal muscle mass, an understanding of the role this hormone plays in young, adult, and old males with normal and low serum testosterone levels is lacking. We investigated the role testosterone plays in the maintenance of muscle mass by examining the effect of orchiectomy-induced testosterone depletion in C57Bl6 male mice at ages ranging from early postnatal through old age (1.5-, 5-, 12-, and 24-month old mice). Following 28 days of testosterone depletion, we assessed mass and fiber cross-sectional-area (CSA) of the tibialis anterior, gastrocnemius, and quadriceps muscles. In addition, we measured global rates of protein synthesis and degradation using the SuNSET method, western blots, and enzyme activity assays. Twenty-eight days of testosterone depletion resulted in reduced muscle mass in the two youngest cohorts, but had no effect in the two oldest cohorts. Mean CSA decreased only in the youngest cohort and only in the tibialis anterior muscle. Testosterone depletion resulted in a general increase in proteasome activity at all ages. No change in protein synthesis was detected at the terminal time point. These data suggest that within physiological serum concentrations, testosterone may not be critical for the maintenance of muscle mass in mature male mice; however, in young mice testosterone is crucial for normal growth.

Inactivation of PPARβ/δ adversely affects satellite cells and reduces postnatal myogenesis

2015 ◽  
Vol 309 (2) ◽  
pp. E122-E131 ◽  
Author(s):  
Preeti Chandrashekar ◽  
Ravikumar Manickam ◽  
Xiaojia Ge ◽  
Sabeera Bonala ◽  
Craig McFarlane ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cell ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Tibialis Anterior ◽  
Tibialis Anterior Muscle ◽  
Skeletal Muscle Regeneration ◽  
Muscle Weight ◽  
Cross Sectional ◽  
Postnatal Myogenesis

Peroxisome proliferator-activated receptor β/δ ( PPARβ/δ) is a ubiquitously expressed gene with higher levels observed in skeletal muscle. Recently, our laboratory showed (Bonala S, Lokireddy S, Arigela H, Teng S, Wahli W, Sharma M, McFarlane C, Kambadur R. J Biol Chem 287: 12935–12951, 2012) that PPARβ/δ modulates myostatin activity to induce myogenesis in skeletal muscle. In the present study, we show that PPARβ/δ-null mice display reduced body weight, skeletal muscle weight, and myofiber atrophy during postnatal development. In addition, a significant reduction in satellite cell number was observed in PPARβ/δ-null mice, suggesting a role for PPARβ/δ in muscle regeneration. To investigate this, tibialis anterior muscles were injured with notexin, and muscle regeneration was monitored on days 3, 5, 7, and 28 postinjury. Immunohistochemical analysis revealed an increased inflammatory response and reduced myoblast proliferation in regenerating muscle from PPARβ/δ-null mice. Histological analysis confirmed that the regenerated muscle fibers of PPARβ/δ-null mice maintained an atrophy phenotype with reduced numbers of centrally placed nuclei. Even though satellite cell numbers were reduced before injury, satellite cell self-renewal was found to be unaffected in PPARβ/δ-null mice after regeneration. Previously, our laboratory had showed (Bonala S, Lokireddy S, Arigela H, Teng S, Wahli W, Sharma M, McFarlane C, Kambadur R. J Biol Chem 287: 12935–12951, 2012) that inactivation of PPARβ/δ increases myostatin signaling and inhibits myogenesis. Our results here indeed confirm that inactivation of myostatin signaling rescues the atrophy phenotype and improves muscle fiber cross-sectional area in both uninjured and regenerated tibialis anterior muscle from PPARβ/δ-null mice. Taken together, these data suggest that absence of PPARβ/δ leads to loss of satellite cells, impaired skeletal muscle regeneration, and postnatal myogenesis. Furthermore, our results also demonstrate that functional antagonism of myostatin has utility in rescuing these effects.

scholarly journals Association between skeletal muscle mass and mammographic breast density

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kwan Ho Lee ◽  
Seoung Wan Chae ◽  
Ji Sup Yun ◽  
Yong Lai Park ◽  
Chan Heun Park
Keyword(s):  
Breast Cancer ◽  
Skeletal Muscle ◽  
Muscle Mass ◽  
Skeletal Muscle Mass ◽  
Premenopausal Women ◽  
Cross Sectional Study ◽  
Causal Link ◽  
The Body ◽  
Mammographic Breast Density ◽  
Cross Sectional

AbstractMammographic density (MD) of the breast and body mass index (BMI) are inversely associated with each other, but have inconsistent associations with respect to the risk of breast cancer. Skeletal muscle mass index (SMI) has been considered to reflect a relatively accurate fat and muscle percentage in the body. So, we evaluated the relation between SMI and MD. A cross-sectional study was performed in 143,456 women who underwent comprehensive examinations from 2012 to 2016. BMI was adjusted to analyze whether SMI is an independent factor predicting dense breast. After adjustment for confounding factors including BMI, the odds ratios for MD for the dense breasts was between the highest and lowest quartiles of SMI at 2.65 for premenopausal women and at 2.39 for postmenopausal women. SMI was a significant predictor for MD, which could be due to the similar growth mechanism of the skeletal muscle and breast parenchymal tissue. Further studies are needed to understand the causal link between muscularity, MD and breast cancer risk.

Prognostic impact of skeletal muscle volume derived from cross-sectional computed tomography images in breast cancer

2018 ◽  
Vol 172 (2) ◽  
pp. 425-436 ◽  
Author(s):  
Eun Jin Song ◽  
Chan Wha Lee ◽  
So-Youn Jung ◽  
Byeong Nam Kim ◽  
Keun Seok Lee ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Computed Tomography ◽  
Skeletal Muscle ◽  
Muscle Volume ◽  
Prognostic Impact ◽  
Cross Sectional ◽  
Computed Tomography Images

Beneficial effects of cod protein on skeletal muscle repair following injury

2012 ◽  
Vol 37 (3) ◽  
pp. 489-498 ◽  
Author(s):  
Junio Dort ◽  
Amélie Sirois ◽  
Nadine Leblanc ◽  
Claude H. Côté ◽  
Hélène Jacques
Keyword(s):  
Skeletal Muscle ◽  
Muscle Mass ◽  
Tibialis Anterior Muscle ◽  
Muscle Growth ◽  
Peanut Protein ◽  
Muscle Repair ◽  
Post Injury ◽  
Cross Sectional ◽  
Resolution Of Inflammation ◽  
Mass Recovery

This study examined the effect of peanut and cod proteins on post-damage skeletal muscle repair, compared with casein. We hypothesized that because of their high arginine content, these proteins would improve the resolution of inflammation and muscle mass recovery following injury. One hundred and twenty-eight male Wistar rats were assigned to isoenergetic diets composed of casein and peanut (experiment 1) or cod protein (experiment 2). After 21 days of feeding, one tibialis anterior muscle (TA) was injured with bupivacaine, while the contralateral TA was injected with saline (sham muscle). Measurements were taken at days 0, 3, 14, and 24 post-injury. Compared with casein, peanut protein reduced muscle mass at days 0 (–12%, p = 0.005) and 14 post-injury in the injured muscle (–13%, p = 0.04), and lowered myofiber cross-sectional area in both the sham (–21%, p = 0.008) and injured muscles (–26%, p = 0.05) at day 24 post-injury, showing that peanut protein has a weak potential to support muscle growth. At day 14 post-injury, muscle mass in the sham (13%, p = 0.02) and injured muscles (12%, p = 0.01) was higher in cod-protein-fed rats, indicating better muscle mass recovery, than in casein-fed rats. Cod protein tended (p = 0.06) to decrease the density of neutrophils (–24%) at day 14 post-injury in the injured muscle, and to decrease the density of ED1+ macrophages at day 24 post-injury in both sham (–29%, p = 0.03) and injured (–40%, p = 0.01) muscles. No effects were observed for peanut protein. These data indicate that cod protein is better for promoting growth and regeneration of skeletal muscle after trauma, partly because of the improved resolution of inflammation.

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