scholarly journals Temporal Profiling of the Cortical Synaptic Mitochondrial Proteome Identifies Ageing Associated Regulators of Stability

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3403
Author(s):  
Laura C. Graham ◽  
Rachel A. Kline ◽  
Douglas J. Lamont ◽  
Thomas H. Gillingwater ◽  
Neil A. Mabbott ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Profiles ◽  
Mitochondrial Protein ◽  
Wild Type Mouse ◽  
Proteomic Profiling ◽  
Temporal Regulation ◽  
Mitochondrial Proteome ◽  
Age Dependent ◽  
Protein Expression Profiles

Synapses are particularly susceptible to the effects of advancing age, and mitochondria have long been implicated as organelles contributing to this compartmental vulnerability. Despite this, the mitochondrial molecular cascades promoting age-dependent synaptic demise remain to be elucidated. Here, we sought to examine how the synaptic mitochondrial proteome (including strongly mitochondrial associated proteins) was dynamically and temporally regulated throughout ageing to determine whether alterations in the expression of individual candidates can influence synaptic stability/morphology. Proteomic profiling of wild-type mouse cortical synaptic and non-synaptic mitochondria across the lifespan revealed significant age-dependent heterogeneity between mitochondrial subpopulations, with aged organelles exhibiting unique protein expression profiles. Recapitulation of aged synaptic mitochondrial protein expression at the Drosophila neuromuscular junction has the propensity to perturb the synaptic architecture, demonstrating that temporal regulation of the mitochondrial proteome may directly modulate the stability of the synapse in vivo.

Comparative proteomic profiling of Hodgkin lymphoma cell lines

2016 ◽  
Vol 12 (1) ◽  
pp. 219-232 ◽  
Author(s):  
D. Vergara ◽  
P. Simeone ◽  
S. De Matteis ◽  
S. Carloni ◽  
P. Lanuti ◽  
...  
Keyword(s):  
Protein Expression ◽  
B Cell ◽  
Hodgkin Lymphoma ◽  
Cell Lines ◽  
Expression Profiles ◽  
Lymphoma Cell ◽  
Classical Hodgkin Lymphoma ◽  
Proteomic Profiling ◽  
Hodgkin Lymphoma Cell ◽  
Protein Expression Profiles

Classical Hodgkin lymphoma models of T- and B-cell derivation show significant differences in their protein expression profiles.

Age-dependent alterations in CRMP2 and CRMP4 protein expression profiles in cat visual cortex

2006 ◽  
Vol 1088 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Lieselotte Cnops ◽  
Tjing-Tjing Hu ◽  
Kalina Burnat ◽  
Estel Van der Gucht ◽  
Lutgarde Arckens
Keyword(s):  
Visual Cortex ◽  
Protein Expression ◽  
Expression Profiles ◽  
Cat Visual Cortex ◽  
Age Dependent ◽  
Protein Expression Profiles

Age-dependent Disturbances of Neuronal and Glial Protein Expression Profiles in Areas of Secondary Neurodegeneration Post-stroke

Neuroscience ◽  
2018 ◽  
Vol 393 ◽  
pp. 185-195 ◽  
Author(s):  
Murielle G. Kluge ◽  
Kimberley Jones ◽  
Lin Kooi Ong ◽  
Emma K. Gowing ◽  
Michael Nilsson ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Profiles ◽  
Post Stroke ◽  
Age Dependent ◽  
Protein Expression Profiles

Investigation of mitochondrial protein expression profiles of Yarrowia lipolytica in response to citric acid production

2020 ◽  
Vol 43 (9) ◽  
pp. 1703-1715
Author(s):  
Luana Vieira da Silva ◽  
Maria Alice Zarur Coelho ◽  
Márcia Regina Soares da Silva ◽  
Priscilla Filomena Fonseca Amaral
Keyword(s):  
Citric Acid ◽  
Protein Expression ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Expression Profiles ◽  
Mitochondrial Protein ◽  
Citric Acid Production ◽  
Protein Expression Profiles

scholarly journals The PAK Inhibitor PF-3758309 Promotes the Inhibitory Effects of Multiple Chemotherapeutic Reagents on Patient-Derived Pancreatic Cancer Cell Lines

2018 ◽  
Author(s):  
Kai Wang ◽  
Nhi Huynh ◽  
Xiao Wang ◽  
Marina Pajic ◽  
Ashleigh Parkin ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Lines ◽  
Tumour Growth ◽  
Pancreatic Cancer Cell ◽  
Expression Profiles ◽  
Ductal Adenocarcinoma ◽  
Inhibitory Effects ◽  
Protein Levels ◽  
Protein Expression Profiles

Pancreatic ductal adenocarcinoma (PDA) remains the most lethal malignancy due to lack of an effective treatment. P21-activated kinases (PAKs) play key roles in PDA growth, and the PAK inhibitor PF-3758309 synergistically reduced PDA growth with gemcitabine. The aim of this study was to determine the effect of PF-3758309 with multiple chemotherapeutic reagents on a panel of patient-derived PDA cell lines. Cells were treated with PF-3758309 plus or minus gemcitabine, 5-fluorouracil (5-FU) or abraxane, and cell proliferation was determined. Protein expression profiles were measured by Western blot. PDA cells were subcutaneously injected into the flanks of SCID mice which were then treated with PF-3758309, gemcitabine, PF-3758309 plus gemcitabine, or gemcitabine plus abraxane. Tumour growth was measured by volume and weight. PF-3758309 enhanced the inhibitory effects of 5-FU, gemcitabine and abraxane on a panel of patient-derived PDA cells, inhibited HIF-1 protein expression and reduced the protein levels of palladin and -SMA in these cells. The combination of PF-3758309 with gemcitabine maximally inhibited PDA growth in vivo, which was comparable to the combination of gemcitabine with abraxane. PF-3758309 enhanced the suppressive effects of multiple chemotherapeutic reagents on the growth of a panel of patient-derived PDA cell lines. The combination of PF-3758309 with gemcitabine provides a potential treatment option with less toxicity than gemcitabine plus abraxane.

scholarly journals Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 308
Author(s):  
Ying-Ray Lee ◽  
Chia-Ming Chang ◽  
Yuan-Chieh Yeh ◽  
Chi-Ying F. Huang ◽  
Feng-Mao Lin ◽  
...  
Keyword(s):  
Protein Expression ◽  
Western Blotting ◽  
Expression Profiles ◽  
Plaque Assay ◽  
Virus Titer ◽  
Enterovirus 71 ◽  
Luciferase Reporter ◽  
Pathogen Invasion

Honeysuckle (Lonicera japonica Thunb) is a traditional Chinese medicine (TCM) with an antipathogenic activity. MicroRNAs (miRNAs) are small non-coding RNA molecules that are ubiquitously expressed in cells. Endogenous miRNA may function as an innate response to block pathogen invasion. The miRNA expression profiles of both mice and humans after the ingestion of honeysuckle were obtained. Fifteen overexpressed miRNAs overlapped and were predicted to be capable of targeting three viruses: dengue virus (DENV), enterovirus 71 (EV71) and SARS-CoV-2. Among them, let-7a was examined to be capable of targeting the EV71 RNA genome by reporter assay and Western blotting. Moreover, honeysuckle-induced let-7a suppression of EV71 RNA and protein expression as well as viral replication were investigated both in vitro and in vivo. We demonstrated that let-7a targeted EV71 at the predicted sequences using luciferase reporter plasmids as well as two infectious replicons (pMP4-y-5 and pTOPO-4643). The suppression of EV71 replication and viral load was demonstrated in two cell lines by luciferase activity, RT-PCR, real-time PCR, Western blotting and plaque assay. Furthermore, EV71-infected suckling mice fed honeysuckle extract or inoculated with let-7a showed decreased clinical scores and a prolonged survival time accompanied with decreased viral RNA, protein expression and virus titer. The ingestion of honeysuckle attenuates EV71 replication and related pathogenesis partially through the upregulation of let-7a expression both in vitro and in vivo. Our previous report and the current findings imply that both honeysuckle and upregulated let-7a can execute a suppressive function against the replication of DENV and EV71. Taken together, this evidence indicates that honeysuckle can induce the expression of let-7a and that this miRNA as well as 11 other miRNAs have great potential to prevent and suppress EV71 replication.

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