scholarly journals A Comprehensive Analysis of Northern versus Liquid Hybridization Assays for mRNAs, Small RNAs, and miRNAs Using a Non-Radiolabeled Approach

2021 ◽  
Vol 43 (2) ◽  
pp. 457-484
Author(s):  
Waqar Ahmad ◽  
Bushra Gull ◽  
Jasmin Baby ◽  
Farah Mustafa
Keyword(s):  
Small Rnas ◽  
Cost Effective ◽  
Northern Blotting ◽  
Locked Nucleic Acid ◽  
Rna Detection ◽  
Effective Manner ◽  
Highly Sensitive ◽  
Hands On ◽  
Efficient Transfer ◽  
Biotinylated Probes

Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity. With the emergence of the field of microRNAs (miRNAs), the necessity for sensitive and quantitative NBs has again emerged. Here, we developed highly sensitive yet non-radiolabeled, fast, economical NB, and liquid hybridization (LH) assays without radioactivity or specialized reagents like locked nucleic acid (LNA)- or digoxigenin-labeled probes for mRNAs/small RNAs, especially miRNAs using biotinylated probes. An improvised means of hybridizing oligo probes along with efficient transfer, cross-linking, and signal enhancement techniques was employed. Important caveats of each assay were elaborated upon, especially issues related to probe biotinylation, use of exonuclease, and bioimagers not reported earlier. We demonstrate that, while the NBs were sensitive for mRNAs and small RNAs, our LH protocol could efficiently detect these and miRNAs using less than 10–100 times the total amount of RNA, a sensitivity comparable to radiolabeled probes. Compared to NBs, LH was a faster, more sensitive, and specific approach for mRNA/small RNA/miRNA detection. A comparison of present work with six seminal studies is presented along with detailed protocols for easy reproducibility. Overall, our study provides effective platforms to study large and small RNAs in a sensitive, efficient, and cost-effective manner.

A Study on the Use of Interactive Multimedia Courseware in the Learning of Rat Dissection

1998 ◽  
Vol 26 (3) ◽  
pp. 235-242 ◽  
Author(s):  
Johnson Y. K. Ngai ◽  
K. S. Chan
Keyword(s):  
Large Class ◽  
Development Process ◽  
Interactive Multimedia ◽  
Cost Effective ◽  
Evaluation Study ◽  
Multimedia Technology ◽  
Traditional Media ◽  
Effective Manner ◽  
Hands On ◽  
Biology Laboratory

Traditionally, students learned rat dissection through reading printed dissection guides, observing teacher demonstration, and hands-on dissection practice in a Biology laboratory. Teacher demonstration was always not very effective for a large class of students. Hands-on practice consumed a lot of rats before students could master the basic skills of rat dissection. Expenditure in terms of time and resources was high in the learning of rat dissection. To help students learn rat dissection in a more cost-effective manner, the authors designed and developed an interactive multimedia courseware. In addition, the authors had made an innovative use of multimedia technology to perform assessment in a manner which was not possible with traditional media. This article summarized the findings of an evaluation study of the interactive multimedia courseware. The evaluation study looked into students' attitude toward the interactive multimedia courseware, and the problems encountered during the use of the courseware. Lastly, the authors' reflection of the development process and product courseware also constituted part of the evaluation.

scholarly journals Pre-vaccination testing could expand coverage of two-dose COVID vaccines

2021 ◽  
Vol 6 ◽  
pp. 105
Author(s):  
Carl A. B. Pearson ◽  
Sam Clifford ◽  
Juliet R. C. Pulliam ◽  
Rosalind M. Eggo
Keyword(s):  
Vaccine Coverage ◽  
Point Of Care ◽  
Vaccine Dose ◽  
Cost Effective ◽  
Antibody Testing ◽  
Past Infection ◽  
Effective Manner ◽  
Highly Sensitive ◽  
Prior Infection ◽  
Antibody Tests

Recent evidence indicates that a single dose of mRNA-based vaccines produce similar immune responses in people with evidence of past infection compared with two doses in immunologically naive individuals. For COVID-19 vaccines with two dose regimens, point-of-care antibody testing for prior infection when administering the first dose could enable expanded vaccine access in a cost-effective manner. Generally, antibody tests with sensitivity and specificity well below that typically accepted for product licensure would still enable expanded vaccine coverage, though to be cost-beneficial total test cost (i.e. procurement and administration) needs to be less than roughly a third of total vaccine dose cost. For highly sensitive (90%) and specific (99%) tests, coverage could be expanded by more than 33%. Tests with the appropriate performance characteristics are plausible, though likely need setting specific tailoring.

Evaluation of an Algorithm for Electronic Surveillance of Hospital-Acquired Infections Yielding Serial Weekly Point Prevalence Scores

2014 ◽  
Vol 35 (7) ◽  
pp. 888-890 ◽  
Author(s):  
Roel H. R. A. Streefkerk ◽  
Gerard J. J. M. Borsboom ◽  
Conrad P. van der Hoeven ◽  
Margreet C. Vos ◽  
Roel P. Verkooijen ◽  
...  
Keyword(s):  
Medical Center ◽  
Cost Effective ◽  
Point Prevalence ◽  
Software System ◽  
Hospital Acquired Infections ◽  
Effective Manner ◽  
Highly Sensitive ◽  
Integrated Software ◽  
Hospital Acquired ◽  
Electronic Hospital

Surveillance of hospital-acquired infections can be approximated by repeated surveys that are performed in a standardized, cost-effective manner. We developed an integrated software system for serial electronic hospital-wide point prevalence surveys using algorithms that proved highly sensitive and specific over a 5-year period in a large university medical center.Infect Control Hosp Epidemiol 2014;35(7):888–890

A Regional Slocum Glider Network in the Mid-Atlantic Bight Leverages Broad Community Engagement

2010 ◽  
Vol 44 (6) ◽  
pp. 185-195 ◽  
Author(s):  
Oscar Schofield ◽  
Josh Kohut ◽  
Scott Glenn ◽  
Julio Morell ◽  
Jorge Capella ◽  
...  
Keyword(s):  
Cost Effective ◽  
Test Bed ◽  
Underwater Gliders ◽  
Response Planning ◽  
Effective Manner ◽  
Hands On ◽  
New Sensors ◽  
Ocean Observing ◽  
Broad Community ◽  
The U.S

AbstractAutonomous underwater gliders have proven to be a cost-effective technology for measuring the 3-D ocean and now represent a critical component during the design and implementation of the Mid-Atlantic Regional Ocean Observing System (MARCOOS), a Region of the U.S. Integrated Ocean Observing System. The gliders have been conducting regional surveys of the Mid-Atlantic (MA) Bight, and during the 3 years of MARCOOS, the glider fleet has conducted 22 missions spanning 10,867 km and collecting 62,824 vertical profiles of data. In addition to collecting regional data, the gliders have facilitated collaboration for partners outside of MARCOOS. The existence of the MA glider observatory provided a unique test bed for cyber-infrastructure tools being developed as part of the National Science Foundation’s Ocean Observatory Initiative. This effort allowed the Ocean Observatory Initiative software to integrate the MARCOOS assets and provided a successful demonstration of an ocean sensor net. The hands-on experience of the MA glider technicians supported training and provided assistance of collaborators within the Caribbean Regional Association, also a region of the U.S. Integrated Ocean Observing System, to assess the efficacy of gliders to resolve internal waves. Finally, the glider fleet has enabled sensor development and testing in a cost-effective manner. Generally, new sensors were tested within the MARCOOS domain before they were deployed in more extreme locations throughout the world’s oceans. On the basis of this experience, the goal of the MARCOOS glider team will be to expand the MA network in coming years. The potential of how an expanded network of gliders might serve national needs was illustrated during the 2010 Macondo Gulf of Mexico oil spill, where gliders from many institutions collected subsurface mesoscale data to support regional models and oil response planning. The experience gained over the last 5 years suggests that it is time to develop a national glider network.

Strategic planning in grassland farming: Principles andapplications

1997 ◽  
pp. 191-197
Author(s):  
W.J. Parker ◽  
N.M. Shadbolt ◽  
D.I. Gray
Keyword(s):  
Strategic Planning ◽  
Performance Indicators ◽  
Cost Effective ◽  
Key Performance Indicators ◽  
Strategic Plans ◽  
Effective Manner ◽  
Farm Business ◽  
Pastoral Farming ◽  
Financial Environment

Three levels of planning can be distinguished in grassland farming: strategic, tactical and operational. The purpose of strategic planning is to achieve a sustainable long-term fit of the farm business with its physical, social and financial environment. In pastoral farming, this essentially means developing plans that maximise and best match pasture growth with animal demand, while generating sufficient income to maintain or enhance farm resources and improvements, and attain personal and financial goals. Strategic plans relate to the whole farm business and are focused on the means to achieve future needs. They should be routinely (at least annually) reviewed and monitored for effectiveness through key performance indicators (e.g., Economic Farm Surplus) that enable progress toward goals to be measured in a timely and cost-effective manner. Failure to link strategy with control is likely to result in unfulfilled plans. Keywords: management, performance

scholarly journals Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 186
Author(s):  
Jia-Huan Qu ◽  
Karen Leirs ◽  
Remei Escudero ◽  
Žiga Strmšek ◽  
Roman Jerala ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Fluorescent Protein ◽  
Cost Effective ◽  
Nitrilotriacetic Acid ◽  
Antibody Fragments ◽  
Rapid Screening ◽  
Red Fluorescent Protein ◽  
Effective Manner ◽  
Surface Regeneration ◽  
Sensitivity Specificity

To date, surface plasmon resonance (SPR) biosensors have been exploited in numerous different contexts while continuously pushing boundaries in terms of improved sensitivity, specificity, portability and reusability. The latter has attracted attention as a viable alternative to disposable biosensors, also offering prospects for rapid screening of biomolecules or biomolecular interactions. In this context here, we developed an approach to successfully regenerate a fiber-optic (FO)-SPR surface when utilizing cobalt (II)-nitrilotriacetic acid (NTA) surface chemistry. To achieve this, we tested multiple regeneration conditions that can disrupt the NTA chelate on a surface fully saturated with His6-tagged antibody fragments (scFv-33H1F7) over ten regeneration cycles. The best surface regeneration was obtained when combining 100 mM EDTA, 500 mM imidazole and 0.5% SDS at pH 8.0 for 1 min with shaking at 150 rpm followed by washing with 0.5 M NaOH for 3 min. The true versatility of the established approach was proven by regenerating the NTA surface for ten cycles with three other model system bioreceptors, different in their size and structure: His6-tagged SARS-CoV-2 spike fragment (receptor binding domain, RBD), a red fluorescent protein (RFP) and protein origami carrying 4 RFPs (Tet12SN-RRRR). Enabling the removal of His6-tagged bioreceptors from NTA surfaces in a fast and cost-effective manner can have broad applications, spanning from the development of biosensors and various biopharmaceutical analyses to the synthesis of novel biomaterials.

scholarly journals Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2940
Author(s):  
Antonella Curulli
Keyword(s):  
Food Industry ◽  
Electrochemical Sensors ◽  
Cost Effective ◽  
Electrochemical Biosensors ◽  
Food Chains ◽  
Sensing Systems ◽  
Highly Sensitive ◽  
Skilled Personnel ◽  
Recent Developments ◽  
Key Issues

Safety and quality are key issues for the food industry. Consequently, there is growing demand to preserve the food chain and products against substances toxic, harmful to human health, such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices, such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long analyses, expensive and complex procedures, and skilled personnel. Therefore, developing performant electrochemical biosensors can significantly support the screening of food chains and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in food screening and their challenges.

scholarly journals Postnatal management of children with antenatal hydronephrosis

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Mohammed S. ElSheemy
Keyword(s):  
Renal Function ◽  
Urinary Tract Obstruction ◽  
Cost Effective ◽  
Voiding Cystourethrography ◽  
Main Body ◽  
Antenatal Hydronephrosis ◽  
Postnatal Management ◽  
Effective Manner ◽  
Lower Urinary Tract Obstruction

Abstract Background Postnatal management of infants with antenatal hydronephrosis (ANH) is still one of the most controversial issues. The majority of infants with ANH are asymptomatic with only few children who develop renal insufficiency. Thus, the biggest challenge for pediatric urologists is to distinguish children who will require further investigations and possible intervention prior to the development of symptoms, complications or renal damage in a cost effective manner without exposing them to the hazards of unnecessary investigations. Main body In this review article, literature on ANH were reviewed to present the current suggestions, recommendations, guidelines and their rational for postnatal management of ANH. It is agreed that a large portion of infants with ANH will improve; thus, the protocol of management is based mainly on observation and follow-up by ultrasound to detect either resolution, stabilization or worsening of hydronephrosis. The first 2 years of life are critical for this follow-up as the final picture is mostly reached during that period. Advanced imaging using voiding cystourethrography or renal scintigraphy are required for children at risk. Then, surgical intervention is selected only for a subgroup of these infants who showed worsening of hydronephrosis or renal function. Conclusions The protocol of management is based mainly on observation and follow-up by US to detect either resolution, stabilization or worsening of hydronephrosis. Postnatal evaluation should be performed for any neonate with a history ANH at any stage during pregnancy even if it was resolved during third trimester. Exclusion of UTI should be performed by urinalysis for all cases followed by urine culture if indicated. Serum creatinine should be performed especially in patients with bilateral ANH. US is the initial standard diagnostic imaging technique. Other imaging modalities like VCUG and nuclear renal scans may be required according to the results of the US evaluation. The most important items in decision making are the presence of bilateral or unilateral hydronephrosis, presence or absence of hydroureter, presence of lower urinary tract obstruction and degree of hydronephrosis on the initial postnatal US. Then an intervention is selected only for a subgroup of these patients who showed deterioration in renal function or degree of hydronephrosis or were complicated by UTIs. All these recommendations are based on the available literature. However, management of ANH is still a controversial issue due to lack of high evidence-based recommendations. Randomised controlled studies are still needed to provide a high level evidence for different aspects of management.

scholarly journals An Artificial-Intelligence-Discovered Functional Ingredient, NRT_N0G5IJ, Derived from Pisum sativum, Decreases HbA1c in a Prediabetic Population

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1635
Author(s):  
Sweeny Chauhan ◽  
Alish Kerr ◽  
Brian Keogh ◽  
Stephanie Nolan ◽  
Rory Casey ◽  
...  
Keyword(s):  
Artificial Intelligence ◽  
Pisum Sativum ◽  
Cost Effective ◽  
Glycated Haemoglobin ◽  
Glucose Regulation ◽  
Functional Ingredient ◽  
Effective Manner ◽  
Human Skeletal Muscle Cells ◽  
Increased Risk

The prevalence of prediabetes is rapidly increasing, and this can lead to an increased risk for individuals to develop type 2 diabetes and associated diseases. Therefore, it is necessary to develop nutritional strategies to maintain healthy glucose levels and prevent glucose metabolism dysregulation in the general population. Functional ingredients offer great potential for the prevention of various health conditions, including blood glucose regulation, in a cost-effective manner. Using an artificial intelligence (AI) approach, a functional ingredient, NRT_N0G5IJ, was predicted and produced from Pisum sativum (pea) protein by hydrolysis and then validated. Treatment of human skeletal muscle cells with NRT_N0G5IJ significantly increased glucose uptake, indicating efficacy of this ingredient in vitro. When db/db diabetic mice were treated with NRT_N0G5IJ, we observed a significant reduction in glycated haemoglobin (HbA1c) levels and a concomitant benefit on fasting glucose. A pilot double-blinded, placebo controlled human trial in a population of healthy individuals with elevated HbA1c (5.6% to 6.4%) showed that HbA1c percentage was significantly reduced when NRT_N0G5IJ was supplemented in the diet over a 12-week period. Here, we provide evidence of an AI approach to discovery and demonstrate that a functional ingredient identified using this technology could be used as a supplement to maintain healthy glucose regulation.

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