scholarly journals An Efficient Bioemulsifier-Producing Bacillus subtilis UCP 0146 Isolated from Mangrove Sediments

2018 ◽  
Vol 2 (4) ◽  
pp. 58 ◽  
Author(s):  
Patrícia Maia ◽  
Vanessa Santos ◽  
Adriana Ferreira ◽  
Marcos Luna ◽  
Thayse Silva ◽  
...  
Keyword(s):  
Surface Tension ◽  
Bacillus Subtilis ◽  
Engine Oil ◽  
Textile Dye ◽  
Mangrove Sediments ◽  
Ionic Charge ◽  
Emulsification Index ◽  
Charge Stability ◽  
Cassava Wastewater

In this work, we investigated the potential of Bacillus subtilis UCP 0146 in the bioconversion of a medium containing 100% cassava flour wastewater to obtain a bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE24) and the surface tension (ST). The ionic charge, stability (temperature, salinity, and pH measured by IE24 and viscosity), and ability to remove and disperse oil and textile dye were investigated. B. subtilis produced an anionic bioemulsifier in the medium containing 100% cassava wastewater under Condition 4 of the factorial design (inoculum 9% at a temperature of 35 °C and shaken at 100 rpm), and showed a surface tension of 39 mN/m, an IE24 of 95.2%, and a yield of 2.69 g·L−1. The bioemulsifier showed stability at different pH (2–8), temperatures (0–120 °C), and NaCl concentrations, a dispersion oil displacement area (ODA) test of 55.83 cm2, and a reduction of the viscosity of the burned engine oil (90.5 Cp). The bioemulsifier was able to remove petroleum (94.4%) and methylene blue azo dye (62.2%). The bioemulsifier and its synthesis from bacteria also emphasizes the role of surfactants in oil remediation.

scholarly journals An Efficient Producing-Bioemulsifier by Bacillus Subtilis UCP 0146 Isolated from Mangrove Sediments

2018 ◽  
Author(s):  
Patrícia. C.V.S. Maia ◽  
Vanessa P. Santos ◽  
Adriana S Ferreira ◽  
Marcos A.C. Luna ◽  
Thayse A. L. Silva ◽  
...  
Keyword(s):  
Surface Tension ◽  
Bacillus Subtilis ◽  
Engine Oil ◽  
Textile Dye ◽  
Mangrove Sediments ◽  
Ionic Charge ◽  
Emulsification Index ◽  
Charge Stability ◽  
Cassava Wastewater

In this work was investigated the potential of Bacillus subtilis UCP 0146 in the bioconversion of the medium containing 100% of cassava flour wastewater to obtain bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE24) and surface tension (TS). The ionic charge, stability (temperature, salinity and pH measured by IE24 and viscosity), ability to remove and disperse oil and textile dye were investigated. B.subtilis produced an anionic bioemulsifier in the medium containing 100% of cassava wastewater in condition 4 of the factorial design (9% of the inoculum, at 35 °C and agitation of 100 rpm) with surface tension of 39mN/m, IE24 of 95.2 % and yield 2.69 g.L-1. Stability at different pH (2-8), temperatures (0-120ºC) and NaCl, dispersed (55.83 cm2-ODA) and reduced the viscosity of the burned engine oil (90.5 cP) , removed 94.4% petroleum and demonstrated efficiency in methylene blue removal (62.2%). The bioemulsifier and its synthesis from bacteria and also emphases on the role of surfactants in oil remediation.

scholarly journals Biosurfactants Production Ability of Lactic Acid Bacteria Strains, Emulsification Activity and Cell Adhesion to Hydrocarbons

2020 ◽  
Vol 70 (12) ◽  
pp. 4493-4498
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Lactic Acid Bacteria ◽  
Engine Oil ◽  
Bacterial Cells ◽  
Ionic Charge ◽  
Polluted Soils ◽  
Emulsification Activity ◽  
Emulsification Index ◽  
Hydrocarbon Degraders

The paper presents the results of research carried out for screening of 10 lactic acid bacteria strains with antifungal activity for their ability to produce biosurfactants and to select the most performant strains for further biotechnological application as hydrocarbon-degraders in bioremediation of oil-polluted soils. All the strains of lactic acid bacteria were able to form circle displacement area in engine oil 2T with various diameters, confirming the production of biosurfactants in various amounts. Emulsification activity was registered, with values of emulsification index E24 ranging from minimum 25.75% for the strain LAB 132 to maximum 75.93% for the strain LAB 13. Bacterial cells presented adhesion to hydrocarbons (kerosene), degrees of hydrophobicity variable, ranged between 25,57% for LAB 58 and 75,42% for Lpl and formed emulsions stable for over 7 days. Results of the assay of ionic charge revealed the anionic nature of the biosurfactants. Keywords: biosurfactants, lactic acid bacteria, emulsification, hydrophobicity, cell adhesion to hydrocarbons

Surfactant Production by Bacillus subtilis Using Very Low Grade and Cheap Substrate

2020 ◽  
Author(s):  
Niranjan Koirala ◽  
Sareeta Khanal ◽  
Sujan Chaudhary ◽  
Sagar Gautam ◽  
Shiv Nandan Sah ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Engine Oil ◽  
Contaminated Site ◽  
Mustard Oil ◽  
Low Grade ◽  
Cell Free Extract ◽  
Emulsification Index ◽  
Mustard Oil Cake ◽  
Wide Range ◽  
Surface Active

Bio-surfactants are surface-active molecules which are produced by the wide range of microbes including bacteria, fungi, and yeast. This study was conducted to identify bio-surfactants by Bacillus subtilis combined with use of cheap substrates and industrial wastes (Mustard cake, Whey and Soya cake) which are found locally in Nepal. Bacillus subtilis, one of the most potential bio-surfactants producer; was isolated from soil sample of hydrocarbon contaminated site. Isolates were grown in a Minimal Salt Media (MSM) with 10% (v/v) mustard oil cake, whey and soya cake separately. The presence and potential of surfactant was determined by the oil spreading technique, emulsification index (%E24) and surface tension measurement. It was revealed that the surface tensions of cell free extract were 54.41, 60.02 and 56.64 mN/m for from mustard cake, whey and soya cake respectively as compared to distilled water (72.09) at 25oC. The emulsification index values are was found to be highest in engine oil from the bio-surfactant extracted from mustard cake, soya cake and whey respectively. Similarly, mustard oil showed the lowest value of emulsification index. The highest emulsification activity was shown in mustard oil i.e. 1.13 from the cell free extract from mustard oil and lowest in engine oil i.e., 0.07, by the extract from soya cake medium, when measured in spectrophotometer at 540 nm. In conclusion, strain of Bacillus subtilis was found to be the potential surface active agent producers on the mustard oil cake, which can be useful medium for various environmental, food and industrial processes.

Directive Effect of Chain Length in Modulating Peptide Nano-assemblies

2020 ◽  
Vol 27 (9) ◽  
pp. 923-929
Author(s):  
Gaurav Pandey ◽  
Prem Prakash Das ◽  
Vibin Ramakrishnan
Keyword(s):  
Electron Microscopy ◽  
Amino Acids ◽  
Light Scattering ◽  
Chain Length ◽  
Self Assembly ◽  
The Self ◽  
Ionic Charge ◽  
Self Assembling ◽  
Biophysical Techniques

Background: RADA-4 (Ac-RADARADARADARADA-NH2) is the most extensively studied and marketed self-assembling peptide, forming hydrogel, used to create defined threedimensional microenvironments for cell culture applications. Objectives: In this work, we use various biophysical techniques to investigate the length dependency of RADA aggregation and assembly. Methods: We synthesized a series of RADA-N peptides, N ranging from 1 to 4, resulting in four peptides having 4, 8, 12, and 16 amino acids in their sequence. Through a combination of various biophysical methods including thioflavin T fluorescence assay, static right angle light scattering assay, Dynamic Light Scattering (DLS), electron microscopy, CD, and IR spectroscopy, we have examined the role of chain-length on the self-assembly of RADA peptide. Results: Our observations show that the aggregation of ionic, charge-complementary RADA motifcontaining peptides is length-dependent, with N less than 3 are not forming spontaneous selfassemblies. Conclusion: The six biophysical experiments discussed in this paper validate the significance of chain-length on the epitaxial growth of RADA peptide self-assembly.

scholarly journals Optimization for enhanced ecofriendly decolorization and detoxification of Reactive Blue160 textile dye by Bacillus subtilis

2020 ◽  
Vol 28 ◽  
pp. e00522
Author(s):  
Selvaraj Barathi ◽  
K.N. Aruljothi ◽  
Chinnannan Karthik ◽  
Indra Arulselvi Padikasan
Keyword(s):  
Bacillus Subtilis ◽  
Textile Dye

On the Role of Marangoni Effects on the Critical Heat Flux for Pool Boiling of Binary Mixtures

1996 ◽  
Vol 118 (1) ◽  
pp. 103-109 ◽  
Author(s):  
W. R. McGillis ◽  
V. P. Carey
Keyword(s):  
Surface Tension ◽  
Heat Flux ◽  
Binary Mixtures ◽  
Critical Heat Flux ◽  
Full Range ◽  
Pool Boiling ◽  
Marangoni Effect ◽  
Restoring Force ◽  
Marangoni Effects

The Marangoni effect on the critical heat flux (CHF) condition in pool boiling of binary mixtures has been identified and its effect has been quantitatively estimated with a modified model derived from hydrodynamics. The physical process of CHF in binary mixtures, and models used to describe it, are examined in the light of recent experimental evidence, accurate mixture properties, and phase equilibrium revealing a correlation to surface tension gradients and volatility. A correlation is developed from a heuristic model including the additional liquid restoring force caused by surface tension gradients. The CHF condition was determined experimentally for saturated methanol/water, 2-propanol/water, and ethylene glycol/water mixtures, over the full range of concentrations, and compared to the model. The evidence in this study demonstrates that in a mixture with large differences in surface tension, there is an additional hydrodynamic restoring force affecting the CHF condition.

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