scholarly journals Analysis of Microbial Communities and Pathogen Detection in Domestic Sewage Using Metagenomic Sequencing

Diversity ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 6
Author(s):  
Muhammad Yasir

Wastewater contains diverse microbes, and regular microbiological screening at wastewater treatment plants is essential for monitoring the wastewater treatment and protecting environmental health. In this study, a metagenomic approach was used to characterize the microbial communities in the influent and effluent of a conventional domestic sewage treatment plant in the metropolitan city of Jeddah. Bacteria were the prevalent type of microbe in both the influent and effluent, whereas archaea and viruses were each detected at <1% abundance. Greater diversity was observed in effluent bacterial populations compared with influent, despite containing similar major taxa. These taxa consisted primarily of Proteobacteria, followed by Bacteroidetes and Firmicutes. Metagenomic analysis provided broad profiles of 87 pathogenic/opportunistic bacteria belonging to 47 distinct genera in the domestic sewage samples, with most having <1% abundance. The archaea community included 20 methanogenic genera. The virus-associated sequences were classified mainly into the families Myoviridae, Siphoviridae, and Podoviridae. Genes related to resistance to antibiotics and toxic compounds, gram-negative cell wall components, and flagellar motility in prokaryotes identified in metagenomes from both types of samples. This study provides a comprehensive understanding of microbial communities in influent and effluent samples of a conventional domestic sewage treatment plant and suggests that metagenomic analysis is a feasible approach for microbiological monitoring of wastewater treatment.

2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.


2014 ◽  
Vol 955-959 ◽  
pp. 3393-3399 ◽  
Author(s):  
Wei Zheng ◽  
Yan Ming Yang ◽  
Yun Long Li ◽  
Jian Qiu Zheng

The process technique and design parameters of project of Solar Ozonic Ecological Sewage Treatment Plant (short for SOESTP) which consists of anaerobic reactor, horizontal subsurface flow (HSSF) constructed wetlands(CWs) and the combination of solar power and ozone disinfection are described, the paper further examines the removal efficiency for treating rural domestic sewage, running expense and recycling ability of product water. The results show that the average percentage removal values of CODcr,BOD5,SS,TN,NH3-N,TP range from 95.6% to 98.0%, 96.0% to 98.7%, 93.1% to 96.1%, 97.0% to 98.9%, 96.9% to 99.5%, 98.2% to 99.6%, respectively, the reduction of fecal coliform (FC) reaches 99.9%, the effluent quality meets the first level A criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant(GB18918-2002). The running cost of SOESTP is 0.063yuan/ m3, saves much more than traditional sewage treatment, and the ozone water obtained from the reservoir will be an ideal choice for disinfection .The system has characteristics of easy manipulation, low operating cost, achieving advanced water, energy conservation and environment protection, is thought to be very suitable for use as the promotion of rural small - scale sewage treatment.


2021 ◽  
Vol 766 (1) ◽  
pp. 012076
Author(s):  
Huang Jin ◽  
Zhang Xiaoxin ◽  
Sun Youfeng ◽  
Huang Xia ◽  
Wang Guanjun

1973 ◽  
Vol 2 (4) ◽  
pp. 473-482 ◽  
Author(s):  
J. C. Van Loon ◽  
J. Lichwa ◽  
D. Ruttan ◽  
J. Kinrade

2013 ◽  
Vol 807-809 ◽  
pp. 1241-1244
Author(s):  
Ying He ◽  
Zhong Xian You ◽  
Yi Jun Ji ◽  
Ya Fei Zhao

Refractory organic pollutants are wastewaters main pollutants in Tianjin TEDA modern industrial park. For clearing up refractory organic pollutants, we must improve the biological process of the sewage treatment plant. This paper focuses on how to use bioaugmentation technology to process the refractory organic pollutants which comes from wastewater of the district. The paper will record data and complete analysis of treatment effect for improving the biological process of sewage treatment plant and make advices.


2013 ◽  
Vol 579-580 ◽  
pp. 798-803
Author(s):  
Qi Zhu ◽  
Zhi Wen Feng

Sewage pumping station is pumping station import at the sewage treatment plant. Its role is to collect the wastewater from the sewage pipe network to subsequent wastewater treatment process facilities. The water level is usually low in sewage collection pipe network. And many wastewater treatment facilities is based on elevation settings. So it is necessary to use lift pump to improve lift of the collected sewage, using high gravity sewage errands subsequent process in order to reduce energy consumption. The traditional way of pumping generally has the shortcomings: the waste of power resources, low efficiency, poor reliability, low degree of automation, which seriously affects the urban sewage treatment capacity. This paper, views pump station control system at the sewage treatment plant as the background, researches frequency control constant pressure control system based on PLC, realizes the energy efficient, automated and reliable direction of development of the sewage pumping station.


2014 ◽  
Vol 1079-1080 ◽  
pp. 480-483
Author(s):  
Li Wang

The graduation design topic for a sewage treatment plant processesdesign - inverted AAO process in durian. Main task is tantamount to designaccording to the requirement of the nature of the city sewage, sewage, scalepreliminary design to complete sewage treatment plant and single processing structure design.


2017 ◽  
Vol 35 (1) ◽  
pp. 141-148 ◽  
Author(s):  
Michał Marzec

AbstractThe reliability of removal of selected contaminants in three technological solutions of the household sewage treatment plants was analysed in this paper. The reliability of the sewage treatment plant with activated sludge, sprinkled biological deposit and hybrid reactor (activated sludge and immersed trickling filter) was analyzed. The analysis was performed using the Weibull method for basic indicators of impurities, BOD5, COD and total suspended solids. The technological reliability of the active sludge treatment plant was 70% for BOD5, 87% for COD and 66% for total suspended solids. In the sewage treatment plant with a biological deposit, the reliability values determined were: 30% (BOD5), 60% (COD) and 67% (total suspended solids). In a treatment plant with a hybrid reactor, 30% of the BOD5and COD limit values were exceeded, while 30% of the total suspended solids were exceeded. The reliability levels are significantly lower than the acceptable levels proposed in the literature, which means that the wastewater discharged from the analysed wastewater treatment plants often exceeds the limit values of indicators specified in currently valid in Poland Regulation of the Minister of Environment for object to 2000 population equivalent.


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