Paramecium bursaria—A Complex of Five Cryptic Species: Mitochondrial DNA COI Haplotype Variation and Biogeographic Distribution

Ciliates are a diverse protistan group and many consist of cryptic species complexes whose members may be restricted to particular biogeographic locations. Mitochondrial genes, characterized by a high resolution for closely related species, were applied to identify new species and to distinguish closely related morphospecies. In the current study, we analyzed 132 sequences of COI mtDNA fragments obtained from P. bursaria species collected worldwide. The results allowed, for the first time, to generate a network of COI haplotypes and demonstrate the relationships between P. bursaria strains, as well as to confirm the existence of five reproductively isolated haplogroups. The P. bursaria haplogroups identified in the present study correspond to previously reported syngens (R1, R2, R3, R4, and R5), thus we decided to propose the following binominal names for each of them: P. primabursaria, P. bibursaria, P. tribursaria, P. tetrabursaria, and P. pentabursaria, respectively. The phylogeographic distribution of P. bursaria species showed that P. primabursaria and P. bibursaria were strictly Eurasian, except for two South Australian P. bibursaria strains. P. tribursaria was found mainly in Eastern Asia, in two stands in Europe and in North America. In turn, P. tetrabursaria was restricted to the USA territory, whereas P. pentabursaria was found in two European localities.

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Six new species of Tonsilla Wang & Yin, 1992 (Araneae: Agelenidae) from southern China, with the first description of the male of T. yanlingensis (Zhang, Yin & Kim, 2000)

Zootaxa ◽

10.11646/zootaxa.5091.2.7 ◽

2022 ◽

Vol 5091 (2) ◽

pp. 357-372

Author(s):

MENG ZHANG ◽

MUHAMMAD IRFAN ◽

LU-YU WANG ◽

ZHI-SHENG ZHANG

Keyword(s):

New Species ◽

Related Species ◽

Southern China ◽

New Combination ◽

Distribution Map ◽

Closely Related Species ◽

First Time

Six new species of the spider genus Tonsilla Wang & Yin, 1992 are described from southern China: T. jinyunensis sp. nov. (♂♀) from Chongqing, T. jiugongensis sp. nov. (♂♀) from Hubei, T. subtruculenta sp. nov. (♂♀), T. rutunda sp. nov. (♂♀) and T. yueliangensis sp. nov. (♂) from Guizhou and T. subrostrum sp. nov. (♂♀) from Hunan. In addition, T. yanlingensis (Zhang, Yin & Kim, 2000) is redescribed and its male is described here for the first time. Tonsilla subyanlingensis Liu & Xu, 2020 is transferred to Draconarius Ovtchinnikov, 1999, hence the new combination Draconarius subyanlingensis (Liu & Xu, 2020) comb. nov. is established. The female of T. subyanlingensis Liu & Xu, 2020 should be treated as T. yanlingensis (Zhang, Yin & Kim, 2000). Detailed descriptions, photographs of copulatory organs and somatic features, a distribution map and comparisons with closely related species are presented.

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A revision of the subgenus Leucandrena of the genus Andrena of eastern Asia (Hymenoptera: Apoidea: Andrenidae)

Zootaxa ◽

10.11646/zootaxa.2145.1.2 ◽

2009 ◽

Vol 2145 (1) ◽

pp. 36-46

Author(s):

HUANLI XU ◽

OSAMU TADAUCHI

Keyword(s):

New Species ◽

East Asian ◽

Eastern Asia ◽

New Synonyms ◽

Two New Species ◽

First Time

The subgenus Leucandrena of the genus Andrena of eastern Asia is revised, and seven species are recognized. Two new species, Andrena (Leucandrena) paramelanospila and Andrena (Leucandrena) banchan are described from Beijing and Xizang (China) respectively, and the male of Andrena (Leucandrena) melanospila Cockerell is described for the first time. A. (Euandrena) taisetsusana Tadauchi et Hirashima, and A. (Andrena) dolharubang Tadauchi and Xu are recognized as new synonyms of the Holarctic A. barbilabris (Kirby). A key to East Asian Leucandrena is given.

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A new species of snake eel, Pisodonophis sangjuensis (Anguilliformes: Ophichthidae) from Korea

Zootaxa ◽

10.11646/zootaxa.2758.1.4 ◽

2011 ◽

Vol 2758 (1) ◽

pp. 57 ◽

Cited By ~ 8

Author(s):

HWAN-SUNG JI ◽

JIN-KOO KIM

Keyword(s):

Mitochondrial Dna ◽

New Species ◽

12S Rrna ◽

Pectoral Fin ◽

Closely Related Species ◽

Dorsal Fin ◽

Posterior Nostril ◽

South Sea ◽

A New Species ◽

Rrna Sequences

A new snake eel, Pisodonophis sangjuensis, is described based on 24 specimens collected from the South Sea of Korea (water depth 5–100 m) between 2005 and 2010. This species is characterized by the following morphological features: fleshy protrusions before and behind the posterior nostril; 1–2 regular rows of conical teeth in both jaws, prevomer and vomer are slightly separated from each other; the origin of the dorsal fin above the middle of the pectoral fin; and the pectoral fin is rounded and not elongated. Pisodonophis sangjuensis is most similar to P. cancrivorus in morphology, but the two species differ in their teeth shape (conical in P. sangjuensis vs. granular in P. cancrivorus), and their numbers of vertebrae (143–153 vs. 153–164, respectively). Pisodonophis sangjuensis differs from P. boro in the origin of the dorsal fin (above the middle of the pectoral fin in P. sangjuensis vs. far behind the pectoral fin in P. boro), their numbers of vertebrae (143–153 vs. 170–177, respectively), and their teeth shape (conical in P. sangjuensis vs. granular in P. boro). Pisodonophis sangjuensis is also easily distinguishable morphologically from the remaining seven Pisodonophis spp. worldwide. Molecular analysis using mitochondrial DNA 12S rRNA sequences supported that P. sangjuensis is a new species because of the considerable genetic distance from what appears to be its most closely related species, P. cancrivorus (d=0.068).

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Revision of world Ceroptresini (Hymenoptera: Cynipidae) with the description of a new genus and five new species

Zootaxa ◽

10.11646/zootaxa.4685.1.1 ◽

2019 ◽

Vol 4685 (1) ◽

pp. 1-67 ◽

Cited By ~ 3

Author(s):

IRENE LOBATO-VILA ◽

JULI PUJADE-VILLAR

Keyword(s):

New Species ◽

Type Species ◽

Morphological Traits ◽

New Genus ◽

Taxonomic Revision ◽

Valid Species ◽

Distribution Data ◽

The Status ◽

The Usa ◽

First Time

A taxonomic revision of the tribe Ceroptresini (Hymenoptera: Cynipidae) is conducted for the first time. Prior to this study, the total number of valid species of Ceroptres, the only genus within Ceroptresini to date, was 23. As a result of this revision, 15 Ceroptres species are retained as valid and one species, Amblynotus ensiger Walsh, 1864, is desynonymized from Ceroptres petiolicola (Osten-Sacken, 1861), being considered here as a valid Ceroptres species: C. ensiger (Walsh, 1864) status verified and comb. nov. An additional five new species are described from Mexico: Ceroptres junquerasi Lobato-Vila & Pujade-Villar sp. nov.; C. lenis Lobato-Vila & Pujade-Villar sp. nov.; C. mexicanus Lobato-Vila & Pujade-Villar sp. nov.; C. nigricrus Lobato-Vila & Pujade-Villar sp. nov.; C. quadratifacies Lobato-Vila & Pujade-Villar sp. nov., increasing the total number of valid Ceroptres species to 21. Ceroptres masudai Abe, 1997 is synonymized with C. kovalevi Belizin, 1973. Ceroptres niger Fullaway, 1911 is transferred to Andricus (Andricus confusus Lobato-Vila & Pujade-Villar comb. nov. and nom. nov.). Five species (Amblynotus inermis Walsh, 1864; Cynips quercusarbos Fitch, 1859; Cynips querficus Fitch, 1859; Cynips quercuspisum Fitch, 1859; and Cynips quercustuber Fitch, 1859) are not considered as valid Ceroptres. The status of Ceroptres quereicola (Shinji, 1938), previously classified as an unplaced species, is commented on. In addition, a Nearctic species from the USA, Ceroptres politus Ashmead, 1896, is here proposed as the type species of a new genus within Ceroptresini: Buffingtonella Lobato-Vila & Pujade-Villar gen. nov. Redescriptions, biological and distribution data, illustrations and keys to genera and species within Ceroptresini are provided. The diagnostic morphological traits of Ceroptresini, Ceroptres and the new genus are discussed.

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DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau

BMC Evolutionary Biology ◽

10.1186/s12862-020-01718-0 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Tai Wang ◽

Yan-ping Zhang ◽

Zhuo-yu Yang ◽

Zhe Liu ◽

Yan-yan Du

Keyword(s):

New Species ◽

Cryptic Species ◽

Dna Barcoding ◽

Related Species ◽

Native Species ◽

Molecular Methods ◽

Tibet Plateau ◽

Closely Related Species ◽

Northeastern Part ◽

Qinghai Tibet Plateau

Abstract Background The northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs). Results After careful identification and comparison of the morphology and DNA barcoding of 1630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions. Conclusions The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

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New species of the genus Epiphragma Osten Sacken from Yunnan, Southwest China (Diptera, Limoniidae)

Zootaxa ◽

10.11646/zootaxa.2121.1.5 ◽

2009 ◽

Vol 2121 (1) ◽

pp. 44-56 ◽

Cited By ~ 3

Author(s):

MENG MAO ◽

DING YANG

Keyword(s):

New Species ◽

Related Species ◽

Southwest China ◽

Closely Related Species ◽

First Time

The subgenus Epiphragma (Epiphragma) Osten Sacken, 1860 is recorded from Yunnan (Southwest China) for the first time. The following five species with two dark rings on the femora are described as new to science: E. mediale sp. nov., E. yunnanense sp. nov., E. ancistrum sp. nov., E. breve sp. nov. and E. elongatum sp. nov. Their relationships with closely related species are discussed. A key to the species of the subgenus Epiphragma from China is presented for the first time.

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DNA Barcoding Reveals Cryptic Diversity in the Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau

10.21203/rs.3.rs-42121/v1 ◽

2020 ◽

Author(s):

tai wang ◽

Yanping Zhang ◽

Zhuoyu Yang ◽

Zhe Liu ◽

Yanyan Du

Keyword(s):

New Species ◽

Cryptic Species ◽

Dna Barcoding ◽

Related Species ◽

Native Species ◽

Molecular Methods ◽

Tibet Plateau ◽

Ecological Value ◽

Closely Related Species ◽

Qinghai Tibet Plateau

Abstract Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) is one of the areas where the number of species of plateau loach is the largest. As one of the three major groups of fishes distributed on the QTP, plateau loach have very important ecological value. However, their taxonomy and systematics are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and there are many cryptic species. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the taxonomic characteristics of the plateau loach by means of morphology, DNA barcoding and multiple species demarcation methods to accurately describe species and allocate taxonomic units to unknown specimens. Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, 20 of which were considered valid local species and two of which were new species that had not been described. Based on the combination of morphological and molecular methods, a total of 24 native species have been found, two of which are cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes, which allow them to be reliably identified. The remaining cases involved 10 closely related species, some of which were rapidly differentiated, had a disputed taxonomic status, or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially in closely related species such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

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Notes on the leaf insects of the genus Phyllium of Sumatra and Java, Indonesia, including the description of two new species with purple coxae (Phasmatodea, Phylliidae)

ZooKeys ◽

10.3897/zookeys.913.49044 ◽

2020 ◽

Vol 913 ◽

pp. 89-126 ◽

Cited By ~ 3

Author(s):

Royce T. Cumming ◽

Sarah Bank ◽

Stephane Le Tirant ◽

Sven Bradler

Keyword(s):

Cryptic Species ◽

Morphological Difference ◽

Nuclear Gene ◽

Molecular Data ◽

Mitochondrial Genes ◽

Peninsular Malaysia ◽

The Other ◽

Two New Species ◽

First Time ◽

Two Populations

Within the last two years, the leaf insects of the genus Phyllium of both the islands of Java and Sumatra have been reviewed extensively based on morphological observations. However, cryptic species which cannot be differentiated morphologically may be present among the various populations. Since it has frequently been demonstrated that analyses based on molecular data can bring clarity in such cases, we conducted a phylogenetic analysis based on three genes (nuclear gene 28S and mitochondrial genes COI and 16S) from the Phyllium species of these islands. The results show distinct molecular divergence for several populations and suggest the presence of two new cryptic species, morphologically inseparable from Phyllium hausleithneri Brock, 1999. From Sumatra, the population originally thought to be a range expansion for Phyllium hausleithneri, is now here described as Phyllium nisussp. nov., with the only consistent morphological difference being the color of the eggs between the two populations (dark brown in P. hausleithneri and tan in P. nisussp. nov.). Further, an additional population with purple coxae from Java was morphologically examined and found to have no consistent features to separate it morphologically from the other purple coxae species. This cryptic species from Java was however shown to be molecularly distinct from the other purple coxae populations from Sumatra and Peninsular Malaysia and is here described as Phyllium gardabagusisp. nov. In addition, Phyllium giganteum is here officially reported from Java for the first time based on both historic and modern records of male specimens.

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Taxonomic study on the photid amphipods (Senticaudata, Corophiida, Photoidea, Photidae) from Korean waters, with descriptions of a new genus and seven new species

ZooKeys ◽

10.3897/zookeys.886.38511 ◽

2019 ◽

Vol 886 ◽

pp. 1-59

Author(s):

Tae Won Jung ◽

Charles Oliver Coleman ◽

Seong Myeong Yoon

Keyword(s):

New Species ◽

New Genus ◽

Posterior Lobe ◽

Closely Related Species ◽

Korean Waters ◽

The Family ◽

Three New Species ◽

First Time ◽

A New Species ◽

Japan And China

In this paper, seven new species of the family Photidae from Korean waters are described and illustrated in detail. Among them, Exiliphotis petilasp. nov. is a monotype of the newly reported Exiliphotisgen. nov., which is characterized by slenderer and more elongate pereopods 5–7 than those of other genera of the family Photidae. The genus Latigammaropsis is reported for the first time from Korean waters based on the description of Latigammaropsis careocavatasp. nov., which is differentiated from other Gammaropsis group by having weakly sexual dimorphic gnathopods 2, those have simple palmar margins in both sexes. Three new species of Photis are also described: Photis broncasp. nov., Photis posterolobussp. nov., and Photis longicarpussp. nov. The formerly misidentified Photis longicaudata from Japan and China as well as the Korean material could be classified as a new species, P. broncasp. nov. For that, the syntypes of P. longicaudata were re-examined, and lectotype and paralectotypes were newly designated in this study. Photis broncasp. nov. is characterized by quadrate tooth on the palmar margin medially on gnathopod 2 in both sexes. Photis posterolobussp. nov. shows a pointed posterior lobe on the ischium and a well-developed process of the propodus on male gnathopod 2. Photis longicarpussp. nov. can be distinguished from other species of the genus by very elongate carpus of male gnathopod 1. Two new species belonging to the genus Podoceropsis are also reported: Podoceropsis insinuomanussp. nov. has a strongly bisinuous palmar margin on male gnathopod 2, and Podoceropsis pseudoclavapessp. nov. differs from the closely related species of Podoceropsis clavapes by different shape of the palmar margin and shorter dactylus of male gnathopod 2. Additionally, a key to the Korean species of Photidae is provided.

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Discovery of a Cryptic Species, Erysiphe Salicina Sp. Nov., and Reconstruction of The Phylogeny of Powdery Mildews On Populus and Salix Spp.

10.21203/rs.3.rs-1199630/v1 ◽

2022 ◽

Author(s):

Jing Feng ◽

Shu-Yan Liu ◽

Uwe Braun ◽

Susumu Takamatsu ◽

Michael Bradshaw ◽

...

Keyword(s):

New Species ◽

Cryptic Species ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Closely Related Species ◽

Rdna Sequences ◽

Powdery Mildews ◽

Salix Gracilistyla ◽

28S Rdna Sequences ◽

Salix Spp

Abstract Recently performed phylogenetic-taxonomic analyses of species belonging to Erysiphe sect. Uncinula on willows (Salix spp.) demonstrated a much higher diversity than previously assumed. Phylogenetic analyses and morphological examinations of Chinese Erysiphe collections on Salix abscondita (= S. raddeana), S. sinica and S. taraikensis, all belonging to Salix subgen. Vetrix sect. Vetrix, revealed an additional cryptic species in sister position to the E. salicis clade, which is described as Erysiphe salicina. The new species clearly distinguished from all allied species in morphological and genetical results. The phylogeny of the new species and closely related species on Populus and Salix spp. (Salicaceae) was reconstructed and discussed using a combined alignment of the internal transcribed spacer (ITS) regions and 28S rDNA sequences. The present phylogenetic analyses suggest that the recently described E. salicicola (on Salix gracilistyla in South Korea) has to be reduced to synonymy with E. salicis var. salicis-gracilistylae (º Uncinula salicis-gracilistylae).

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