scholarly journals Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 247
Author(s):  
Andi Setiawan ◽  
Widyastuti Widyastuti ◽  
Arik Irawan ◽  
Oklis Syahrin Wijaya ◽  
Aspita Laila ◽  
...  
Keyword(s):  
Solid State ◽  
Secondary Metabolites ◽  
Solid State Fermentation ◽  
Bioactive Metabolites ◽  
Marine Actinomycetes ◽  
Etoac Extract ◽  
Primary Screening ◽  
Inhibition Concentration ◽  
Shrimp Shell ◽  
Shrimp Shell Waste

Marine actinomycetes are prolific microorganisms; however, knowledge of their diversity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an untapped source of novel bioactive compounds. In this study, we investigated shrimp shell as substrates for model production bioactive metabolites from actinomycetes under solid state fermentation (SSF) conditions. A total of fifteen actinomycetes were isolated from six sponges and one tunicate. The isolated actinomycetes were grown on solid shrimp shells. Cultures of actinomycetes were extracted with ethyl acetate (EtOAc) and extracts were bioassayed for activity against Staphylococcus aureus. One isolate 18A13O1 from the sponge, Rhabdastrella globostellata, exhibited antibacterial activity on primary screening compared to the other samples and was chosen for further study. Visualization using SEM showed aerial and substrate mycelia. Through phylogenetic analysis, it was confirmed that isolate 18A13O1 is a Pseudonocardia carboxydivorans. Purification of an EtOAc extract yielded A13B2, which showed a minimum inhibition concentration against S. aureus at 15.6 μg/mL. It can be concluded that this basic information is very important for further studies related to the development of the production of bioactive secondary metabolites through the solid state fermentation process.

scholarly journals SECONDARY METABOLITES PRODUCED BY MARINE ACTINOMYCETE STREPTOMYCES SP. G246

2021 ◽  
Vol 59 (1) ◽  
pp. 1
Author(s):  
Doan Thi Mai Huong
Keyword(s):  
Solid State ◽  
Secondary Metabolites ◽  
Solid State Fermentation ◽  
Culture Broth ◽  
Comparison Study ◽  
Streptomyces Sp ◽  
Marine Actinomycete ◽  
Metabolites Production ◽  
Sponge Halichondria Panicea

In a recent study, we described two new lavandulylated flavonoids, along with eight known compounds from the culture broth of a Streptomyces sp. (strain G246), isolated from the sponge Halichondria panicea, collected in the sea of Son Tra peninsula (Da Nang). A comparison study was conducted to differentiate between solid and liquid fermentation technique for secondary metabolites production of strain G246. In this paper, we report the isolation and structural characterization of 9 secondary metabolites (1-9) from strain G246 by solid state fermentation. Compound 3 was the only one similarity between these fermentation techniques.

Recent developments on solid-state fermentation for production of microbial secondary metabolites: Challenges and solutions

2020 ◽  
pp. 124566
Author(s):  
Vinod Kumar ◽  
Vivek Ahluwalia ◽  
Saurabh Saran ◽  
Jitendra Kumar ◽  
Anil Kumar Patel ◽  
...  
Keyword(s):  
Solid State ◽  
Secondary Metabolites ◽  
Solid State Fermentation ◽  
Recent Developments

scholarly journals Effect of the solid state fermentation of cocoa shell on the secondary metabolites, antioxidant activity, and fatty acids

2017 ◽  
Vol 27 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Ozana Almeida Lessa ◽  
Nadabe dos Santos Reis ◽  
Selma Gomes Ferreira Leite ◽  
Melissa Limoeiro Estrada Gutarra ◽  
Alexilda Oliveira Souza ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Solid State ◽  
Secondary Metabolites ◽  
Solid State Fermentation ◽  
Cocoa Shell

scholarly journals Optimization of Protease from Aspergillus Niger under Solid-State Fermentation Utilizing Shrimp Shell Substrate

2021 ◽  
Vol 11 (6) ◽  
pp. 14809-14824
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Proteolytic Enzymes ◽  
Initial Moisture Content ◽  
Inoculum Size ◽  
Protease Production ◽  
Shrimp Shell

Proteases, also known as proteinases or proteolytic enzymes, belong to a group of hydrolases. It can be applied in numerous fields and industries. Solid-state fermentation (SSF) is recognized as an effective method to produce protease. The ultimate aim of this study is to optimize the production of protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as a solid substrate. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for enzyme production is analyzed using statistical software, Minitab 16. A 23 full factorial experimental design was employed, and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content, and 1.0 inoculum size. It is concluded that SSF protease was successfully produced from Aspergillus niger by utilizing shrimp waste as substrate. Through optimization study, moisture content, the interaction between incubation temperature and moisture content, interaction between moisture content and inoculum size significantly impact protease production.

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