scholarly journals Optimization of Propagation Medium for Enhanced Polyhydroxyalkanoate Production by Pseudomonas oleovorans

Fermentation ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 16
Author(s):  
Daniela Chmelová ◽  
Barbora Legerská ◽  
Miroslav Ondrejovič ◽  
Stanislav Miertuš
Keyword(s):  
Biomass Yield ◽  
Synthetic Medium ◽  
Ammonium Phosphate ◽  
Nitrogen Sources ◽  
Pseudomonas Oleovorans ◽  
Promising Alternative ◽  
Carbon And Nitrogen ◽  
Medium Chain Length ◽  
Propagation Medium ◽  
Carbon Excess

Polyhydroxyalkanoates (PHAs) represent a promising alternative to commercially used petroleum-based plastics. Pseudomonas oleovorans is a natural producer of medium-chain-length PHA (mcl-PHA) under cultivation conditions with nitrogen limitation and carbon excess. Two-step cultivation appears to be an efficient but more expensive method of PHA production. Therefore, the aim of this work was to prepare a minimal synthetic medium for maximum biomass yield and to optimize selected independent variables by response surface methodology (RSM). The highest biomass yield (1.71 ± 0.04 g/L) was achieved in the optimized medium containing 8.4 g/L glucose, 5.7 g/L sodium ammonium phosphate and 35.4 mM phosphate buffer. Under these conditions, both carbon and nitrogen sources were completely consumed after 48 h of the cultivation and the biomass yield was 1.7-fold higher than in the conventional medium recommended by the literature. This approach demonstrates the possibility of using two-stage PHA cultivation to obtain the maximum amount of biomass and PHA.

scholarly journals Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14

2010 ◽  
Vol 53 (3) ◽  
pp. 643-652 ◽  
Author(s):  
André Lacerda Ulysses de Carvalho ◽  
Fábio Henrique Portella Corrêa de Oliveira ◽  
Rosa de Lima Ramos Mariano ◽  
Ester Ribeiro Gouveia ◽  
Ana Maria Souto-Maior
Keyword(s):  
Bioactive Compounds ◽  
Biomass Yield ◽  
Anaerobic Respiration ◽  
High Growth ◽  
Nitrogen Sources ◽  
High Growth Rate ◽  
Excess Oxygen ◽  
Carbon And Nitrogen ◽  
High Biomass Yield ◽  
Short Transition

The physiology of B. subtilis R14 was investigated in minimal medium under excess-oxygen and oxygen-limited conditions. Growth and efficient sporulation could be achieved in excess-oxygen culture on medium with readily metabolizable carbon and nitrogen sources, which allowed high growth rate and high biomass yield. A short transition phase between the exponential growth and sporulation could be attained by formulating a medium with a well-balanced C/N ratio. Under oxygen-limitation, but in the presence of essential nutrients (i.e. excess-nutrient cultivation), B. subtilis R14 produced bioactive compounds, which showed activity against several phytopathogenic bacteria. Under anaerobic condition, the organism did not grow neither through fermentation nor anaerobic respiration. However, addition of pyruvate to the medium allowed its growth through fermentation and anaerobic respiration. The knowledge acquired in this work could be relevant both for the design of a production process as well as for the formulation of an effective commercial biocontrol product.

scholarly journals Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

2021 ◽  
Vol 9 (6) ◽  
pp. 1280
Author(s):  
Dana Byrtusová ◽  
Martin Szotkowski ◽  
Klára Kurowska ◽  
Volha Shapaval ◽  
Ivana Márová
Keyword(s):  
Biomass Yield ◽  
Synthetic Medium ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Potassium Nitrate ◽  
Economic Feasibility ◽  
Intracellular Lipids ◽  
Microbial Biotechnology ◽  
Food And Feed ◽  
Valuable Product

Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain R. kratochvilovae CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans.

scholarly journals STUDY ON EFFECT OF PHYSICOCHEMICAL PARAMETERS ON GROWTH OF FUSARIUM EQUISETI (MTCC9658)

2021 ◽  
pp. 53-54
Author(s):  
Arshi Naaj Afsana ◽  
Ajay Kumar Srivastava ◽  
Madhulika Singh
Keyword(s):  
Biomass Production ◽  
Biomass Yield ◽  
Nitrogen Sources ◽  
Fungal Species ◽  
Chemical Parameters ◽  
Fusarium Equiseti ◽  
Carbon And Nitrogen ◽  
Physical Conditions ◽  
Temperature Ranges ◽  
Physical And Chemical

The microorganisms like fungi proliferate in different environmental and ecological conditions depending upon the availability of nutrients and the form of nutrient that they absorb. In addition to this the physical conditions like temperature, pH, salinity and light etc. also effect the growth and biomass production of fungal species. The present study was carried out to determine the effect of variation in physical and chemical parameters on growth and biomass production of the fungal strain Fusarium equiseti (MTCC9658). A maximum biomass yield of 12.24 g/L at pH 5 and temperature of 30ºC was recorded; and minimum of 5.21 g/L at pH 9 and temperature of 25ºC.The observations clearly indicate that pH 9 and above did not support higher biomass production at any of the temperatures,while pH 5 to pH 6 supports higher dry biomass production in all temperature ranges applied.The different carbon and nitrogen sources used in the medium corresponded to variation in growth of the strain. Dextrose in combination with Yeast extract as nitrogen source was best for biomass production.

scholarly journals A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production
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