scholarly journals Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2351
Author(s):  
Anil K. Verma ◽  
Sayanti Mandal ◽  
Aadhya Tiwari ◽  
Chiara Monachesi ◽  
Giulia N. Catassi ◽  
...  
Keyword(s):  
Gene Editing ◽  
Wheat Gluten ◽  
Wheat Variety ◽  
Transgenic Wheat ◽  
Wheat Genome ◽  
Current Status ◽  
Gluten Free ◽  
Breeding Methods ◽  
Wheat Varieties ◽  
Multiple Challenges

Wheat gluten contains epitopes that trigger celiac disease (CD). A life-long strict gluten-free diet is the only treatment accepted for CD. However, very low-gluten wheat may provide an alternative treatment to CD. Conventional plant breeding methods have not been to produce celiac-safe wheat. RNA interference technology, to some extent, has succeeded in the development of safer wheat varieties. However, these varieties have multiple challenges in terms of their implementation. Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease 9 (CRISPR/Cas9) is a versatile gene-editing tool that has the ability to edit immunogenic gluten genes. So far, only a few studies have applied CRISPR/Cas9 to modify the wheat genome. In this article, we reviewed the published literature that applied CRISPR/Cas9 in wheat genome editing to investigate the current status of the CRISPR/Cas9 system to produce a low-immunogenic wheat variety. We found that in recent years, the CRISPR/Cas9 system has been continuously improved to edit the complex hexaploid wheat genome. Although some reduced immunogenic wheat varieties have been reported, CRISPR/Cas9 has still not been fully explored in terms of editing the wheat genome. We conclude that further studies are required to apply the CRISPR/Cas9 gene-editing system efficiently for the development of a celiac-safe wheat variety and to establish it as a “tool to celiac safe wheat.”

scholarly journals Status and Contribution of CRISPR/Cas9 Based Gene-Editing System in the Development of a Low-Immunogenic Wheat Variety

2020 ◽  
Author(s):  
Anil K. Verma ◽  
Aadhya Tiwari ◽  
Elena Lionetti ◽  
Akash Srivastava ◽  
Chiara Monachesi ◽  
...  
Keyword(s):  
Alternative Treatment ◽  
Gene Editing ◽  
Wheat Variety ◽  
Wheat Genome ◽  
Research Articles ◽  
Specific Gene ◽  
Original Research ◽  
Gluten Free ◽  
Wheat Protein ◽  
Included Review

Gluten, a wheat protein, contains epitopes that trigger celiac disease (CD). So far, there is no treatment available for CD other than following a life-long, strict gluten-free diet (GFD). A very low-gluten or gluten-free wheat could provide an alternative treatment to CD. Till date, conventional plant breeding methods are not sufficient to produce celiac-safe wheat. Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 (CRISPR/Cas9) is a versatile gene-editing system may efficiently edit the immunogenic gluten protein thereby producing a celiac-safe wheat variety. However, CRISPR/Cas9-mediated genome editing system has not been widely investigated to edit the wheat genome. Published literature available on various scientific platforms, that applied the CRISPR/Cas9 system to edit the wheat genome were explored. Only original research articles were included. Review articles, protocols, scientific presentations, and Ph.D. thesis were excluded. CRISPR/Cas9 is a highly specific gene-editing technology that can be used to efficiently edit the complex hexaploid wheat genome. It targets a specific wheat genome locus, delete an immunogenic gene and replace it with a preferred gene. CRISPR/Cas9 technology could be a breakthrough in providing an alternative treatment for CD. However, further studies are required to efficiently apply this gene-editing technology to develop a celiac-safe wheat variety.

scholarly journals Consumer Acceptance of Gene-Edited versus Genetically Modified Foods in Korea

2021 ◽  
Vol 18 (7) ◽  
pp. 3805
Author(s):  
Eunae Son ◽  
Song Soo Lim
Keyword(s):  
Gene Editing ◽  
Consumer Preferences ◽  
Genetically Modified ◽  
Relevant Information ◽  
Consumer Acceptance ◽  
Genetically Modified Foods ◽  
Breeding Methods ◽  
Novel Foods ◽  
Novel Technology ◽  
Potential Market

Food made with gene-editing has received considerable attention in recent years because it is claimed to be a little different from traditional genetically modified breeding methods concerning safety. However, consumer acceptance of these novel foods and their potential market uptake remains to be answered. This study aims to assess differences in the acceptance of gene-edited and genetically modified foods in Korea. The choice-based conjoint analysis is adopted to estimate part-worth functions for the soybean oil attributes with 200 surveyed samples. The estimated part-worth values reveal how much each attribute affects consumers’ decision-making. Estimated results suggest that consumers tend to accept gene-editing more than genetically modified foods. The acceptance of novel technology is shown to correspond closely to the degree of consumers’ scientific knowledge, highlighting the importance of revealing relevant information regarding the technology. Results also show that country of origin is a significant food-specific attitudinal factor in shaping consumer preferences.

scholarly journals Prevalence of Wheat/Gluten-Related Disorders and Gluten-Free Diet in Paraguay: An Online Survey-Based Study

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 396
Author(s):  
Noé Ontiveros ◽  
Raúl Emilio Real-Delor ◽  
José Antonio Mora-Melgem ◽  
Carlos Eduardo Beltrán-Cárdenas ◽  
Oscar Gerardo Figueroa-Salcido ◽  
...  
Keyword(s):  
Latin American ◽  
Weight Control ◽  
Online Survey ◽  
Adult Population ◽  
Wheat Gluten ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Questionnaire Response ◽  
Face To Face ◽  
Validated Questionnaire

Gluten-related disorders (GRDs) are increasing around the world, but their magnitude and relevance remain unknown in most Latin American countries. Thus, an online survey was conducted to estimate the prevalence of GRDs as well as adherence to a gluten-free diet (GFD) in Paraguayan adult population. There were 1058 individuals surveyed using a validated questionnaire (response rate of 93.9%). The self-reported prevalence rates were as follows (95% CI): gluten sensitivity (GS), 10.30% (8.53–12.29); non-celiac GS (NCGS), 5.19% (3.94–6.71); physician-diagnosed celiac disease (PD-CD), 3.11% (2.15–4.35); wheat allergy (WA), 2.07% (1.30–3.13); and adherence to GFD, 15.69% (13.55–18.02). Excluding CD, more women than men met the criteria for GRDs, adverse food reactions, and GFD (p < 0.05). Eight respondents reported the coexistence of NCGS with PD-CD and/or WA. Most cases on a GFD indicated medical/dietitian advice for following the diet (68.07%). Non-self-reported GS individuals indicated weight control (46.4%) and the notion that the GFD is healthier (20.2%) as the main motivations for following the diet. GRDs are not uncommon in Paraguayan adult population. It seems that there is awareness about GRDs and the GFD, but training about the diagnosis of GRDs is desirable because of the informed overlapping diagnoses of CD or WA with NCGS. Future studies involving face-to-face interviews are necessary.

scholarly journals The Effects of Plant Breeders’ Rights on Wheat Productivity and Varietal Improvement in South African Agriculture

2019 ◽  
Vol 11 (12) ◽  
pp. 3378
Author(s):  
Charity Ruramai Nhemachena ◽  
Johann F. Kirsten ◽  
Binganidzo Muchara
Keyword(s):  
Intellectual Property ◽  
South African ◽  
Wheat Variety ◽  
Wheat Varieties ◽  
Whole Wheat ◽  
New Varieties ◽  
Varietal Improvement ◽  
African Agriculture ◽  
Protection Index ◽  
Plant Breeders Rights

The strengthening of the intellectual property rights (IPRs) for plant varieties provide incentives for breeding companies to invest more resources in plant breeding. The main objective of this paper was to analyze the effects of strengthening the wheat variety intellectual protection on wheat productivity and the release of new varieties. The strength of IPR systems was measured using an intellectual property (IP) protection index, and plant breeders’ rights (PBRs) granted for wheat varieties. The empirical analyses were based on correlation and multiple regression analyses. The results showed that strengthening IPR systems in South Africa contribute to improving wheat productivity and increasing the number of wheat varieties released. Furthermore, although the robust coefficients of the other IPR variables are positive, they are statistically insignificant for all scenarios. There is a need for more incentives beyond granting PBRs and strengthening of IPR systems to be provided in the whole wheat sector to stimulate increased investments and the release of new varieties.

scholarly journals Effects of Wheat Bug (Eurygasterspp. andAeliaspp.) Infestation in Preharvest Period on Wheat Technological Quality and Gluten Composition

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Aleksandra M. Torbica ◽  
Jasna S. Mastilović ◽  
Milica M. Pojić ◽  
Žarko S. Kevrešan
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Wheat Gluten ◽  
Sodium Dodecyl ◽  
Deformation Energy ◽  
Wheat Varieties ◽  
Gluten Proteins ◽  
Molecular Weights ◽  
Sds Page ◽  
Technological Quality ◽  
Gluten Index

The effects of wheat bug infestation (Eurygasterspp. andAeliaspp.) on the composition of wheat gluten proteins and its influence on flour technological quality were investigated in the present study. Wheat samples of six wheat varieties, collected from two localities in northern Serbia, were characterized by significantly different level of wheat bug infestation. Composition of wheat gluten proteins was determined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE), while the selected parameters of technological quality were determined according to standard and modified empirical rheological methods (Farinograph, Extensograph, Alveograph, and Gluten Index). The surface morphology of the selected samples was viewed using scanning electron microscopy (SEM). Wheat from wheat bug-infested locality regardless of the variety had deteriorated technological quality expressed with higher Farinograph softening degree, lower or immeasurable Extensograph energy, and Alveograph deformation energy. The most important changes in the gluten proteins composition of bug-infested wheat were related to gliadin subunits with molecular weights below 75 kDa, which consequently caused deterioration of uniaxial and biaxial extensibility and dough softening during mixing.

scholarly journals Immunodeficient Rabbit Models: History, Current Status and Future Perspectives

2020 ◽  
Vol 10 (20) ◽  
pp. 7369
Author(s):  
Jun Song ◽  
Brooke Pallas ◽  
Dongshan Yang ◽  
Jifeng Zhang ◽  
Yash Agarwal ◽  
...  
Keyword(s):  
Gene Editing ◽  
Interleukin 2 ◽  
Current Status ◽  
Loss Of Function ◽  
Future Perspectives ◽  
Transcription Activator ◽  
Immune Genes ◽  
Forkhead Box ◽  
Recombination Activating Gene ◽  
Model Family

Production of immunodeficient (ID) models in non-murine animal species had been extremely challenging until the advent of gene-editing tools: first zinc finger nuclease (ZFN), then transcription activator-like effector nuclease (TALEN), and most recently clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR)/Cas9. We and others used those gene-editing tools to develop ID rabbits carrying a loss of function mutation in essential immune genes, such as forkhead box protein N1 (FOXN1), recombination activating gene 1/2 (RAG1/2), and interleukin 2 receptor subunit gamma (IL2RG). Like their mouse counterparts, ID rabbits have profound defects in their immune system and are prone to bacterial and pneumocystis infections without prophylactic antibiotics. In addition to their use as preclinical models for primary immunodeficient diseases, ID rabbits are expected to contribute significantly to regenerative medicine and cancer research, where they serve as recipients for allo- and xeno-grafts, with notable advantages over mouse models, including a longer lifespan and a much larger body size. Here we provide a concise review of the history and current status of the development of ID rabbits, as well as future perspectives of this new member in the animal model family.

scholarly journals Genome editing in large animals: current status and future prospects

2019 ◽  
Vol 6 (3) ◽  
pp. 402-420 ◽  
Author(s):  
Jianguo Zhao ◽  
Liangxue Lai ◽  
Weizhi Ji ◽  
Qi Zhou
Keyword(s):  
Regenerative Medicine ◽  
Genome Editing ◽  
Biomedical Research ◽  
Human Disease ◽  
Gene Editing ◽  
Disease Modeling ◽  
Current Status ◽  
Future Prospects ◽  
Recent Advances ◽  
Large Animals

AbstractLarge animals (non-human primates, livestock and dogs) are playing important roles in biomedical research, and large livestock animals serve as important sources of meat and milk. The recently developed programmable DNA nucleases have revolutionized the generation of gene-modified large animals that are used for biological and biomedical research. In this review, we briefly introduce the recent advances in nuclease-meditated gene editing tools, and we outline these editing tools’ applications in human disease modeling, regenerative medicine and agriculture. Additionally, we provide perspectives regarding the challenges and prospects of the new genome editing technology.

scholarly journals Socio-economic assessment of wheat variety Shatabdi in some selected areas of Bangladesh

1970 ◽  
Vol 34 (2) ◽  
pp. 215-226
Author(s):  
M Elahi Baksh ◽  
Md Jahangir Kabir ◽  
Kalpona Kispatta
Keyword(s):  
Wheat Variety ◽  
Sampling Technique ◽  
Economic Assessment ◽  
Variable Cost ◽  
Gross Margin ◽  
Wheat Varieties ◽  
New Variety ◽  
Pest Infestation ◽  
Farmer Selection ◽  
Yield Difference

 The survey was conducted in two villages under Dinajpur and Thakurgaon Districts during March 2004 to assess the socioeconomic aspects of Shatabdi wheat variety adoption at farm level. Stratified random sampling technique was followed for farmer selection. All categories of farmers expected wheat varieties having high yielding potentialities with less disease and pest infestation, more heat tolerant, and bold grain with golden colour. New wheat variety Shatabdi is able to meet the maximum expected characters. Old variety Kanchan was more disease susceptible and new one was free from disease. A significant yield difference was found between new and old verities (Shatabdi yielded 3l% to 43% higher compared to Kanchan over the locations and farmers group). Gross margin (return over variable cost) of Shatabdi also higher compared to Kanchan. By cultivating new variety, farmers earned additional gross margin of Tk. 6446 to Tk. 8353 per hectare in Jagdal and Tk. 6097 to Tk. 9314 per hectare in Daulatpur over Kanchan. Cent percent farmers over the locations said that their income was increased by cultivating Shatabdi compared to old variety Kanchan. The non-adopter farmers wanted new variety seeds. All groups of farmers wanted training/video show for up-dating their technical know how. Key Words: New vs old varieties; profitability level; farmers' adoption. DOI: 10.3329/bjar.v34i2.5792Bangladesh J. Agril. Res. 34(2): 215-226, June 2009

scholarly journals Genotypic variability for salt stress tolerance among wild and cultivated wheat germplasms at an early development stage

2019 ◽  
Vol 4 (1) ◽  
pp. 375-380
Author(s):  
Imen Klay ◽  
Leila Riahi ◽  
Hajer Slim Amara ◽  
Abderrazak Daaloul
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Common Wheat ◽  
Triticum Turgidum ◽  
Wheat Variety ◽  
Development Stage ◽  
Salt Stress Tolerance ◽  
Wheat Varieties ◽  
The Common ◽  
Photosynthetic Traits

AbstractThis study was conducted to evaluate the variability of salt tolerance potentials among nine wheat genotypes representing wild and cultivated species namely Triticum turgidum subsp. durum, Triticum aestivum and Aegilops geniculata. Ionomic and photosynthetic traits were used for the screening of the studied samples when faced with four salinity levels of NaCl (0, 50, 100 and 150 mM) under green house conditions at the seedling stage. The investigated genotypes exhibited different levels of salt stress tolerance. Ionomic and photosynthetic traits underline the distinctiveness of the common wheat varieties which highlighted particular performances under salt stress conditions and showed higher tolerance potentials among the studied genotypes. Interestingly, the Vaga variety showed more ability to maintain higher K+/Na+ ratios and Pq coefficients compared with the control conditions and stable Fv/F0 and Fv/Fm ratios. Stable behaviour was exhibited by wild Aegilops accessions while durum wheat varieties have been shown to be more sensitive to salt stress. Further investigations were required for the common wheat variety Vaga, which could be useful for successful breeding and biotechnological improvement strategies concerning wheat species.

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