Interaction of Monocyte-Derived Dendritic Cells with Ara h 2 from Raw and Roasted Peanuts

Ara h 2 is a relevant peanut allergen linked to severe allergic reactions. The interaction of Ara h 2 with components of the sensitization phase of food allergy (e.g., dendritic cells) has not been investigated, and could be key to understanding the allergenic potential of this allergen. In this study, we aimed to analyze such interactions and the possible mechanism involved. Ara h 2 was purified from two forms of peanut, raw and roasted, and labeled with a fluorescent dye. Human monocyte-derived dendritic cells (MDDCs) were obtained, and experiments of Ara h 2 internalization by MDDCs were carried out. The role of the mannose receptor in the internalization of Ara h 2 from raw and roasted peanuts was also investigated. Results showed that Ara h 2 internalization by MDDCs was both time and dose dependent. Mannose receptors in MDDCs had a greater implication in the internalization of Ara h 2 from roasted peanuts. However, this receptor was also important in the internalization of Ara h 2 from raw peanuts, as opposed to other allergens such as raw Ara h 3.

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Inhibition of O-GlcNAc Transferase Alters the Differentiation and Maturation Process of Human Monocyte Derived Dendritic Cells

Cells ◽

10.3390/cells10123312 ◽

2021 ◽

Vol 10 (12) ◽

pp. 3312

Author(s):

Matjaž Weiss ◽

Marko Anderluh ◽

Martina Gobec

Keyword(s):

Dendritic Cells ◽

Posttranslational Modification ◽

Human Monocyte ◽

T Cell Differentiation ◽

Maturation Process ◽

Hla Dr ◽

Glcnac Transferase ◽

And Function

The O-GlcNAcylation is a posttranslational modification of proteins regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase. These enzymes regulate the development, proliferation and function of cells, including the immune cells. Herein, we focused on the role of O-GlcNAcylation in human monocyte derived dendritic cells (moDCs). Our study suggests that inhibition of OGT modulates AKT and MEK/ERK pathways in moDCs. Changes were also observed in the expression levels of relevant surface markers, where reduced expression of CD80 and DC-SIGN, and increased expression of CD14, CD86 and HLA-DR occurred. We also noticed decreased IL-10 and increased IL-6 production, along with diminished endocytotic capacity of the cells, indicating that inhibition of O-GlcNAcylation hampers the transition of monocytes into immature DCs. Furthermore, the inhibition of OGT altered the maturation process of immature moDCs, since a CD14medDC-SIGNlowHLA-DRmedCD80lowCD86high profile was noticed when OGT inhibitor, OSMI-1, was present. To evaluate DCs ability to influence T cell differentiation and polarization, we co-cultured these cells. Surprisingly, the observed phenotypic changes of mature moDCs generated in the presence of OSMI-1 led to an increased proliferation of allogeneic T cells, while their polarization was not affected. Taken together, we confirm that shifting the O-GlcNAcylation status due to OGT inhibition alters the differentiation and function of moDCs in in vitro conditions.

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Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells

Molecular Immunology ◽

10.1016/j.molimm.2006.01.007 ◽

2006 ◽

Vol 43 (14) ◽

pp. 2151-2160 ◽

Cited By ~ 66

Author(s):

A LOPEZ ◽

E ALEGRE ◽

J LEMAOULT ◽

E CAROSELLA ◽

A GONZALEZ

Keyword(s):

Dendritic Cells ◽

Human Monocyte ◽

Degradation Pathway ◽

Regulatory Role ◽

Tryptophan Degradation

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Vibrio parahaemolyticus Flagellin Stimulates the Maturation of Human Monocyte-Derived Dendritic Cells and Elicits Th1-Type Immune Response.

Blood ◽

10.1182/blood.v106.11.3872.3872 ◽

2005 ◽

Vol 106 (11) ◽

pp. 3872-3872

Author(s):

Hyun-Kyu Kang ◽

Myong-Suk Park ◽

Shee-Eun Lee ◽

Joon-Haeng Rhee ◽

Jung-Sun Park ◽

...

Keyword(s):

Immune Response ◽

Dendritic Cells ◽

Vibrio Parahaemolyticus ◽

Human Monocyte ◽

Phenotypic Change ◽

Target Cells ◽

Maximal Effect ◽

Dependent Manner ◽

Functional Maturation

Abstract Flagellin, the principal component of bacterial flagella, interacts with Toll-like receptor (TLR5) and induces the generation of a pro-inflammation response and activation of host dendritic cells (DCs) in vivo. In this study, we investigated the role of Vibrio parahaemolyticus (V. parahaemolyticus)-derived flagellin as a DC maturation-inducing molecule. V. parahemolyticus-derived flagellin (100–1,000 ng/ml) induced the maturation of human monocyte-derived dendritic cells in a concentration-dependent manner with maximal effect at 500 ng/ml of flagellin as determined by increased levels of surface markers, namely, CD1a, CD80, CD86, CD83, and HLA-DR, a response which could be compared with the phenotypic change in immature DCs (iDCs) treated with lipopolysaccharide (LPS) or cytokine cocktails (CC) with TNF-α, IL-1β, IL-6, and PGE2. Moreover, V. parahaemolyticus-derived flagellin also reduced phagocytic activity, and increased IL-12 production in a polymyxin B-insensitive manner and DC-mediated T cell proliferation, which is comparable with that of LPS- or CC-treated iDCs at several responder to stimulator ratios, suggesting the functional maturation of DCs by V. parahaemolyticus-derived flagellin. Maturation of DCs by V. parahaemolyticus-derived flagellin also elicited a significant increase in specific cytotoxic activity against target cells at several effector to target cells ratios as determined by 51Cr-release assay, and induced Th1-type immune response, such as increase in INF-γ producing cells, determined by ELISPOT assay and analysis of intracellular cytokine staining assay. Taken together, this study demonstrates the role of V. parahaemolyticus-derived flagellin in the functional maturation of DCs, and suggests that V. parahaemolyticus-derived flagellin as a useful molecule for the development of a DC-based immunotherapy against tumors.

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Publisher Correction: Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac

Scientific Reports ◽

10.1038/s41598-017-16204-9 ◽

2017 ◽

Vol 7 (1) ◽

Author(s):

Tiyash Parira ◽

Gloria Figueroa ◽

Alejandra Laverde ◽

Gianna Casteleiro ◽

Mario E. Gomez Hernandez ◽

...

Keyword(s):

Dendritic Cells ◽

Alcohol Exposure ◽

Human Monocyte ◽

Chronic Alcohol ◽

Post Translational Modifications ◽

Chronic Alcohol Exposure

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DC-SIGN Is the Major Mycobacterium tuberculosis Receptor on Human Dendritic Cells

Journal of Experimental Medicine ◽

10.1084/jem.20021468 ◽

2002 ◽

Vol 197 (1) ◽

pp. 121-127 ◽

Cited By ~ 428

Author(s):

Ludovic Tailleux ◽

Olivier Schwartz ◽

Jean-Louis Herrmann ◽

Elisabeth Pivert ◽

Mary Jackson ◽

...

Keyword(s):

Dendritic Cells ◽

Mycobacterium Tuberculosis ◽

Human Monocyte ◽

Mannose Receptor ◽

Intercellular Adhesion Molecule ◽

Minor Role ◽

Hla Dr ◽

A Minor ◽

Human Dendritic Cells

Early interactions between lung dendritic cells (LDCs) and Mycobacterium tuberculosis, the etiological agent of tuberculosis, are thought to be critical for mounting a protective anti-mycobacterial immune response and for determining the outcome of infection. However, these interactions are poorly understood, at least at the molecular level. Here we show that M. tuberculosis enters human monocyte-derived DCs after binding to the recently identified lectin DC-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN). By contrast, complement receptor (CR)3 and mannose receptor (MR), which are the main M. tuberculosis receptors on macrophages (Mϕs), appeared to play a minor role, if any, in mycobacterial binding to DCs. The mycobacteria-specific lipoglycan lipoarabinomannan (LAM) was identified as a key ligand of DC-SIGN. Freshly isolated human LDCs were found to express DC-SIGN, and M. tuberculosis–derived material was detected in CD14−HLA-DR+DC-SIGN+ cells in lymph nodes (LNs) from patients with tuberculosis. Thus, as for human immunodeficiency virus (HIV), which is captured by the same receptor, DC-SIGN–mediated entry of M. tuberculosis in DCs in vivo is likely to influence bacterial persistence and host immunity.

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Maturation of human monocyte-derived dendritic cells (MoDCs) in the presence of prostaglandin E2 optimizes CD4 and CD8 T cell-mediated responses to protein antigens: role of PGE2 in chemokine and cytokine expression by MoDCs

International Immunology ◽

10.1093/intimm/dxh335 ◽

2005 ◽

Vol 17 (12) ◽

pp. 1561-1572 ◽

Cited By ~ 31

Author(s):

Marie T. Rubio ◽

Terry K. Means ◽

Ronjon Chakraverty ◽

Juanita Shaffer ◽

Yasuhiro Fudaba ◽

...

Keyword(s):

Dendritic Cells ◽

T Cell ◽

Prostaglandin E2 ◽

Human Monocyte ◽

Cd8 T Cell ◽

Cytokine Expression ◽

Protein Antigens

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Role Of Dendritic Cells In Allergic Reactions To Pru P 3

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2009.12.860 ◽

2010 ◽

Vol 125 (2) ◽

pp. AB220

Author(s):

E. Gomez ◽

A. Diaz-Perales ◽

S. Lopez ◽

L. Tordesillas ◽

I. Doña ◽

...

Keyword(s):

Dendritic Cells ◽

Allergic Reactions ◽

Pru P 3

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GSK-3 mediates differentiation and activation of proinflammatory dendritic cells

Blood ◽

10.1182/blood-2006-06-028951 ◽

2006 ◽

Vol 109 (4) ◽

pp. 1584-1592 ◽

Cited By ~ 82

Author(s):

Elena Rodionova ◽

Michael Conzelmann ◽

Eugene Maraskovsky ◽

Michael Hess ◽

Michael Kirsch ◽

...

Keyword(s):

Dendritic Cells ◽

In Vitro Model ◽

Glycogen Synthase ◽

Human Monocyte ◽

Glycogen Synthase Kinase 3 ◽

Inhibitory Effects ◽

Tnf Α ◽

Vitro Model

Abstract The key components of the intracellular molecular network required for the expression of a specific function of dendritic cells (DCs) are as yet undefined. Using an in vitro model of human monocyte-derived DC differentiation, this study investigates the role of glycogen synthase kinase 3 (GSK-3), a multifunctional enzyme critical for cellular differentiation, apoptosis, self-renewal, and motility, in this context. We demonstrate that GSK-3 (1) inhibits macrophage development during differentiation of DCs, (2) is constitutively active in immature DCs and suppresses spontaneous maturation, and (3) acquires a proinflammatory functional status mediating high levels of IL-12, IL-6, and TNF-α secretion, and partially inhibits IL-10 in the context of DC activation. In particular, GSK-3 enhances IL-12p35 mRNA expression and thus the production of the proinflammatory cytokine IL-12p70 by integrating the activities of other kinases priming GSK-3 targets and the inhibitory effects of Akt-1. GSK-3 may therefore act as a key integrator of activating and inhibitory pathways involved in proinflammatory DC differentiation and activation.

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