scholarly journals Enrichment of Olive Fruits in Antioxidant Content by Pre-Harvest Salicylic Acid Treatment

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1513
Author(s):  
Gracia Patricia Blanch ◽  
María C. Gómez-Jiménez ◽  
María Luisa Ruiz del Castillo

We here study the effect of the pre-harvest application of salicylic acid at two different concentrations on the olive phenolic composition. Influence of the cultivar and harvesting day were considered. As a result, the total phenol content increased significantly, particularly when using 200 mg mL−1 of salicylic acid. However, the free radical scavenging activity was cultivar dependent. For instance, when the olives were harvested on day 3 and treated with 200 mg mL−1 of salicylic acid, the antioxidant activity decreased from 161 to 278 µg mL−1 for Arbequina, whereas it increased from 397 to 258 µg mL−1 for Picual. Generally speaking, oleuropein and hydroxytyrosol contents enhanced with the application of 200 mg mL−1 of salicylic acid. The results found suggest that exogenous salicylic acid is an interesting agronomic practice to enrich olive fruits in antioxidants.

2021 ◽  
Author(s):  
Purkha Ram Meghwal ◽  
Dalpat Singh ◽  
Saurabh Swami ◽  
Akath Singh

Abstract Cordia myxa is one of the important underutilized fruit plants suitable for arid and semi-arid regions of India having potential for commercial exploitation in vegetable and pickle industry. Fruits of four improved genotypes of Cordia myxa were analyzed for nutritional value, total phenol content and free radical scavenging activity. Acetone extracts from dried fruit samples were prepared to determine total phenol content and free radical scavenging activity using DPPH essay. The fruits harvested at 20 or 30 days after fruit set recorded higher crude fibre and crude protein as compared to at full ripening stage. The total phenol content was found highest in fruits harvested earliest irrespective of genotypes. The correlation coefficient between total phenol content and free radical scavenging activity of fruit extract was found positive and significant. The results suggest that the fruits of Cordia myxa should be harvested at 20 to 30 days after fruit set to get maximum benefits of its anti-oxidant and other nutritional properties.


Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 165-171
Author(s):  
A. Razzak ◽  
K.R. Roy ◽  
U. Sadia ◽  
H. Mominul ◽  
T. Suvro ◽  
...  

Profile of physicochemical and antioxidant activity of dried Moringa leaves from Bangladesh are presented. Moringa is beneficial for health because it has a lot of nutritional and medicinal values. The leaves were collected and washed with distilled water at different temperatures in an oven dryer, and then the fine powder is taken as a sample by grinding and sieving method. This research was done to compare the changes in physicochemical and antioxidant elements at different temperatures (60°C, 70°C and 80°C) and to find the right temperature at which the nutrient loss will be the lowest. This study showed that as the drying temperature changed, so did the nutrient component of Moringa leaves. Physicochemical parameters (moisture, ash, protein, carbohydrate, fat, color) and antioxidant activity (Total phenol content, DPPH free radical scavenging activity, vitamin C, and ß-carotene) were extracted using a variety of methods. The protein content, carbohydrate content was estimated by the Kjeldahl and phenol sulfuric acid method respectively. Total phenol content (38.30 mg/100g), DPPH (77.79%), and ßcarotene (22.71mg/100g) were measured by the spectrophotometric method. And the colorimeter instrument is used for determining the optical properties. It can be seen that the moisture, ash, protein, carbohydrate, Total phenol content, Vitamin C, DPPH free radical scavenging activity, ß-carotene contents decrease significantly with increasing drying temperature, whereas fat content increases. At 60°C drying temperature the nutrient loss was lowest compared to 70°C and 80°C drying temperature, so it can be concluded that 60°C is the most suitable temperature for drying Moringa leaves.


2020 ◽  
Vol 10 (10) ◽  
pp. 3444
Author(s):  
Alexandros Tzachristas ◽  
Konstantina Pasvanka ◽  
Maria Liouni ◽  
Antony C. Calokerinos ◽  
Panagiotis Tataridis ◽  
...  

This study evaluated the antioxidant capacity, total phenol content, and sensory profile of selected Greek wines made from Vitis vinifera L. c.v. Moschofilero in two consecutive vintages, treated with powdered Hippophae rhamnoides L. leaves (HRL). Radical Scavenging activity, reducing power, total phenol content (TPC), and color intensity increased in a linear manner in relation to HRL treatments. Indicatively the addition of 0.8 g/L of HRL increased the radical scavenging activity as determined via the inhibition of the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical from 28.4 to 55.8% in comparison to the initial values. Equally the reducing power as determined by the ferric reducing antioxidant power (FRAP) assay increased from 35.3 to 62.1%, and total phenol content values increased from 11% to 23.7% and the color intensity increased from 39.9 to 50.7%. The main oenological attributes examined, remained unchanged after the HRL addition. The addition of up to 0.4 g/L of HRL did not have a major impact on the organoleptic characteristics of the wines tasted whereas concentrations higher than 0.8 g/L were not considered beneficial. Results denote that the addition of H. rhamnoides leaves to white wines contributes positively to the overall antioxidant capacity and could be used if authorized as an antioxidant agent in wines vinified in the absence of or in synergy with sulphur dioxide.


2011 ◽  
Vol 396-398 ◽  
pp. 1349-1352
Author(s):  
Jian Hui Wang ◽  
Dong Min Liu ◽  
Yong Le Liu ◽  
Chi Ling Li ◽  
Yuan Yuan Cheng ◽  
...  

This study was designed to determine the polyphenolic contents of Eucommia ulmoicles Oliv leaf extracts and evaluate their antioxidant activities in vitro. All the extracts were evaluated for their radical scavenging activity by the DPPH method and their oxidative-retarding efficiency in vegetable oils. It was observed that the highest values were found in 70% MeOH extracts for total phenolic content as gallic acid. A good correlation between antioxidant activity and total phenol content in the extracts was found. The extraction yield, total phenol content and radical scavenging activity were increased and peroxide formation in vegetable oils were decreased with the graded solvent concentration of MeOH. The phenolic compound yield ranged from 2.45 to 11.35% and the total phenol content increased from 34.51 mg/g to 47.68 mg/g. DPPH scavenging activity increased from 56.32% to 90.37% as MeOH concentration increased. Compared to the control (70.45 meq/kg), 70% MeOH fraction with 47.68 mg/g phenolic compounds shows much stronger antioxidant ability (30.12 meq/kg) in vegetable oil system.


BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5556-5573
Author(s):  
Alma Rosa Agapito-Ocampo ◽  
Benjamín Amaro-Guadarrama ◽  
Gerardo Díaz-Godínez ◽  
Ma de Lourdes Acosta-Urdapilleta ◽  
Liliana Aguilar-Marcelino ◽  
...  

Edible coatings (EC) are a biodegradable alternative for food preservation. In this work EC based on extracellular compounds of Humphreya coffeata and pectin (PHC) were elaborated and characterized through testing on strawberries. Two PHC types were obtained (PHC1, which settled, and PHC2, which floated during the first separation). The conservation period of the strawberry and the phenol content and antioxidant activity in strawberries with the EC were determined. Four EC were prepared (EC1, EC2, EC3, and EC4), in which EC1 did not contain any PHC, EC2 contained PHC1, EC3 contained PHC2, and EC4 contained both PHCs. Three EC types (EC2, EC3, and EC4) delayed the decay of strawberries approximately 90% at 20 °C during the first 10 days of evaluation, and at 4 °C the effect was between 75 and 90% after 25 days of experimentation. However, EC3 and EC4, both of which contained PHC2, presented the best results. The content of total phenols (approximately 55 mg (gallic acid equivalent (GAE)/mL) and the free radical scavenging activity were 35% with ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 75% with DPPH (2,2-diphenyl-1-picrylhydrazyl) in strawberries with EC. Thus, EC are an alternative for preserving strawberries without losing their antioxidant capacity.


Author(s):  
Pinki Pal ◽  
Shiv Bhadra Singh ◽  
Aaditya Singh

 Objective: The present study is designed to evaluate preliminary phytochemical constituents, physiochemical properties evaluation, free radical scavenging activity, and high-performance thin-layer chromatography (HPTLC) fingerprinting analysis of Cucurbita maxima seed oil.Methods: Total phenol content, total flavonoid content, total tannin content, 1, 1-diphenyl, 2-picryl-hydrazyl (DPPH) radical scavenging method, and HPTLC fingerprinting analysis for compounds responsible for antioxidant activity were used.Result: Phytochemical screening shows the presence of constituents such as alkaloids, glycosides, carbohydrates, fixed oil and fats, phytosterols, saponin, phenolic compounds, tannins, proteins, amino acids, gums, and mucilage. Physicochemical studies show that the oil contains an acid value, saponification value, iodine value of 9.53 mg KOH/g, 183 mg KOH/g, and 116.51 mg I2/100 g, respectively. Total phenol content was 3.9498±3.2 mg gallic acid equivalent/g, total flavonoid content was 56.115±4.6 mg rutin equivalent/g, and total tannin content was 19.4±0.40 mg tannic acid equivalent/g. The radical scavenging activity by DPPH Model shows that the methanolic extract has maximum antioxidant activity with IC50 value of 238.31. HPTLC fingerprinting also proofs the presence of phenol and flavonoid compounds in the seed oil.Conclusion: The results in the paper show that the C. maxima seed oil is an natural antioxidant and can be used for the treatment of various disorders.


2019 ◽  
Vol 9 (1) ◽  
pp. 60-69
Author(s):  
Fensia Analda Souhoka ◽  
Yeanchon H Dulanlebit ◽  
Ester M. C Tomasoa

This study aimed to determine phytochemical tests and antioxidant activity of fruit and peel extract of pisang jarum. Sample was extracted with 80% of ethanol, methanol, and acetone using reflux method. The result showed that pisang jarum extract contains phenols, flavonoids, and terpenoids, whereas peel extract contains phenols, flavonoids, terpenoids, alkaloids, and saponins. The total phenol content (TPC) of pisang jarum is 0.0250-0.0316 mg PE/g extract and peel is 0,1756-0,2679 mg PE/g extract. The total flavonoid content (TFC) of pisang jarum is 0.8412-1.4466 mg QE/g extract and peel is 3.2128-5.1073 mg QE/g extract. The antioxidant activity of extract were analyze by DPPH free radical scavenging activity. The antioxidant activities (IC50) of pisang jarum is 1092.92-3871.24 ppm and peel is 310.08-558.07 ppm, so it is classified as a weak antioxidant


2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Tatjana Stanojković ◽  
Marijana Kosanić

The aim of this study was to determine neuroprotective, antioxidant, antimicrobial and cytotoxic potential of acetone extracts of Coprinus comatus and Coprinopsis picacea mushrooms. The neuroprotetive activity was tested against acetylcholinesterase enzyme using the Ellman method. Antioxidant activity was evaluated by free radical scavenging ability, superoxide anion radical scavenging activity and reducing power. The total phenol content was examined using Folin-Ciocalteu reagent. The antimicrobial potential was determined by a microdilution method against 12 microorganisms. The cytotoxic activity was tested using MTT method on the Hela, A549 and LS174 cells. Our results indicate that C. comatus expressed a stronger neuroprotective effect (the percentage of inhibition of acetylcholinesterase was within the range 19.66-51.73%) than C. picacea. In antioxidant effect C. comatus had more potent free radical scavenging activity (IC50 = 276.69 μg/mL) and superoxide anion radical scavenging activity (IC50 = 39.40 μg/mL), while reducing power was relatively similar for both species. The total amount of phenols for C. comatus and C. picacea was 50.57 and 50.20 μg PE/mg of dry extracts, respectively. In antimicrobial activity, C. picacea showed a better effect with MIC values from 0.1 to 7.5 mg/mL. Finally, C. picacea expressed stronger cytotoxicity toward A549 and LS174 cells, while C. comatus was more active against Hela cell.


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