scholarly journals Effect of Barley Antifreeze Protein on Dough and Bread during Freezing and Freeze-Thaw Cycles

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1698
Author(s):  
Xiangli Ding ◽  
Tingting Li ◽  
Hui Zhang ◽  
Chengran Guan ◽  
Jianya Qian ◽  
...  
Keyword(s):  
Loss Modulus ◽  
Antifreeze Protein ◽  
Gas Production ◽  
Yeast Cells ◽  
Bread Dough ◽  
Bread Crumb ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Increase Rate ◽  
Freezing Treatment

In order to verify the cryoprotective effect of an antifreeze protein (BaAFP-1) obtained from barley on bread dough, the effect of BaAFP-1 on the rheological properties, microstructure, fermentation, and baking performance including the proofing time and the specific volume of bread dough and bread crumb properties during freezing treatment and freeze-thaw cycles were analysed. BaAFP-1 reduced the rate of decrease in storage modulus and loss modulus values during freezing treatment and freeze-thaw cycles. It influenced the formation and the shape of ice formed during freezing and inhibited ice recrystallization during freeze-thaw. BaAFP-1 maintained gas production ability and gas retention properties, protected gluten network and the yeast cells from deterioration caused by ice formation and ice crystals recrystallisation in dough samples during freezing treatment and freeze-thaw treatment. It slow down the increase rate of hardness of bread crumb. The average area of pores in bread crumbs decreased significantly (p < 0.05) as the total number of pores increased (p < 0.05), and the addition of BaAFP-1 inhibited this deterioration. These results confirmed the cryoprotective activity of BaAFP-1 in bread dough during freezing treatment and freeze-thaw cycles.

scholarly journals Effects of Pectin on the Physicochemical Properties and Freeze-Thaw Stability of Waxy Rice Starch

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2419
Author(s):  
Yuheng Zhai ◽  
Jiali Xing ◽  
Xiaohu Luo ◽  
Hao Zhang ◽  
Kai Yang ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Loss Modulus ◽  
Rice Starch ◽  
Scanning Calorimetry ◽  
Freeze Thaw ◽  
Textural Parameter ◽  
Waxy Rice ◽  
Pasting Temperature ◽  
Starch Paste ◽  
Waxy Rice Starch

In this study, the effects of the addition of pectin (PEC) on the physicochemical properties and freeze-thaw stability of waxy rice starch (WRS) were investigated. As PEC content increased, the pasting viscosity and pasting temperature of WRS significantly increased (p < 0.05), whereas its breakdown value and setback value decreased. Differential scanning calorimetry showed that the addition of PEC increased the gelatinization temperature of WRS, but decreased its gelatinization enthalpy. Rheological measurements indicated that the addition of PEC did not change the shear-thinning behavior of WRS–PEC blends, and the storage modulus and loss modulus were positively correlated with PEC content. Moreover, the textural parameter of WRS decreased with the increase in PEC content. Furthermore, the addition of PEC decreased the transmittance of starch paste, but enhanced the freeze-thaw stability of WRS to some extent. These results may contribute to the development of WRS-based food products.

scholarly journals Enhancing the Freeze-Thaw Durability of Concrete Through Ice Recrystallization Inhibition by Poly(vinyl Alcohol)

2019 ◽  
Author(s):  
Zhengyao Qu ◽  
Shuaiqi Guo ◽  
Christian C. M. Sproncken ◽  
Romà Surís-Valls ◽  
qingliang yu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Vinyl Alcohol ◽  
Cement Hydration ◽  
Poly Vinyl Alcohol ◽  
Seasonal Temperature ◽  
Ice Recrystallization Inhibition ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Recrystallization Inhibition ◽  
Durability Of Concrete

Frost weathering of porous materials caused by seasonal temperature changes is a major source of damage to the world’s infrastructure and cultural heritage. Here we investigate poly(vinyl alcohol) (PVA) addition as a means to enhance the freeze-thaw durability of concrete without compromising its structural or mechanical integrity. We evaluate the ice recrystallization inhibition activity of PVA in a cementitious environment and the impact of PVA on key structural and mechanical properties, such as cement hydration (products), microstructure, strength, as well as freeze‑thaw resistance. We find that a low amount of PVA significantly reduces the surface scaling of concrete and displays excellent ice recrystallization inhibition in the saturated Ca(OH)<sub>2 </sub>solution which has a similar pH value as cement pore solution, while it does not affect cement hydration, microstructure, nor its mechanical properties. These findings contribute to new insights on freeze-thaw damage mechanism and more importantly we disclose a new direction for the design of concrete with excellent freeze‑thaw resistance.

scholarly journals The Impact of Salts on the Ice Recrystallization Inhibition Activity of Antifreeze (Glyco)Proteins

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 347 ◽  
Author(s):  
Romà Surís-Valls ◽  
Ilja Voets
Keyword(s):  
Cell Types ◽  
Hofmeister Series ◽  
Ice Crystal ◽  
Ice Recrystallization Inhibition ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Type Iii ◽  
Biological Matter ◽  
Recrystallization Inhibition ◽  
The Impact

Antifreeze (glyco)proteins (AF(G)Ps) have received increasing attention as potential cryopreservation agents since their discovery in the 1970s. While cryopreservation strategies for specific cells (such as red blood cells) are successful and widely implemented, preservation of other cell types, tissues and whole organs remains challenging. This is due to the multifactorial nature of the freeze-thaw damage, the complexity of preserving biological matter and the (country-to-country) variability of the employed procedures and regulations. AF(G)Ps are well-known for their ability to modulate ice crystal growth morphology and ice recrystallization inhibition (IRI), both of which are considered key contributors to freeze-thaw damage. To date, however, the impact of AF(G)Ps on cell survival remains at best partially understood as conflicting results on the benefits or disadvantages of including AF(G)P in cryopreservation strategies remain unelucidated. We hypothesize that variability in the additives in the cryopreservation media contributes to the observed discrepancies. To critically examine this idea, we monitored the inhibition of ice recrystallization by AF(G)P in the presence of various salts using a quantitative analysis of optical microscopy images via the Lifshitz-Slyozov-Wagner (LSW) theory for Oswald ripening. We found that the addition of salts, which are used in culture and cryopreservation media, enhances the IRI activity of AF(G)Ps, and that the magnitude of the enhancement was in line with the Hofmeister series. The size of ice crystals grown in AFGP1–5 and type III AFP samples containing chloride, phosphate and citrate ions were statistically smaller after 90 min of incubation than crystals grown in the absence of these salts. The ice recrystallization rates (kd) of AFGP1–5 and type III AFP samples prepared at a fixed overall ionic strength of 100 mM progressively decreased following the Hofmeister series for anions. Our results demonstrate that the performance of AF(G)Ps is significantly influenced by additives present in common cryopreservation media. It is thus important to conduct excipient compatibility experiments to identify potential incompatibilities between additives and AF(G)Ps in cryopreservation formulations.

Prospecting for ice association: characterization of freeze–thaw selected enrichment cultures from latitudinally distant soils

2012 ◽  
Vol 58 (4) ◽  
pp. 402-412 ◽  
Author(s):  
Sandra L. Wilson ◽  
Paul Grogan ◽  
Virginia K. Walker
Keyword(s):  
Microbial Consortia ◽  
Rrna Gene ◽  
Metagenomic Sequencing ◽  
Ice Recrystallization Inhibition ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Recrystallization Inhibition ◽  
And Function ◽  
Selective Regime

Freeze–thaw stress has previously been shown to alter soil community structure and function. We sought to further investigate this stress on enriched microbial consortia with the aim of identifying microbes with ice-associating adaptations that facilitate survival. Enrichments were established to obtain culturable psychrotolerant microbes from soil samples from the latitudinal extremes of the Canadian Shield plateau. The resulting consortia were subjected to consecutive freeze–thaw cycles, and survivors were putatively identified by their 16S rRNA gene sequences. Even though the northerly site was exposed to longer, colder winters and large spring-time temperature fluctuations, the selective regime similarly affected both enriched consortia. Quantitative PCR and metagenomic sequencing were used to determine the frequency of a subset of the resistant microbes in the original enrichments. The metagenomes showed 22 initial genera, only 6 survived and these were not dominant prior to selection. When survivors were assayed for ice recrystallization inhibition and ice nucleation activities, over 60% had at least one of these properties. These phenotypes were not more prevalent in the northern enrichment, indicating that regarding these adaptations, the enrichment strategy yielded seemingly functionally similar consortia from each site.

scholarly journals Protection of Alcohol Dehydrogenase against Freeze–Thaw Stress by Ice-Binding Proteins Is Proportional to Their Ice Recrystallization Inhibition Property

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 638
Author(s):  
Young Hoon Lee ◽  
Kitae Kim ◽  
Jun Hyuck Lee ◽  
Hak Jun Kim
Keyword(s):  
Alcohol Dehydrogenase ◽  
Binding Proteins ◽  
Residual Activity ◽  
Fluorescence Emission ◽  
Fluorescence Emission Spectra ◽  
Ice Recrystallization Inhibition ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Ice Binding ◽  
Recrystallization Inhibition

Ice-binding proteins (IBPs) have ice recrystallization inhibition (IRI) activity. IRI property has been extensively utilized for the cryopreservation of different types of cells and tissues. Recent reports demonstrated that IRI can also play a significant role in protecting proteins from freezing damage during freeze–thaw cycles. In this study, we hypothesized that the protective capability of IBPs on proteins against freeze–thaw damage is proportional to their IRI activity. Hence we used two IBPs: one with higher IRI activity (LeIBP) and the other with lower activity (FfIBP). Yeast alcohol dehydrogenase (ADH) was used as a freeze-labile model protein. IBPs and ADH were mixed, frozen at −20 °C, and thawed repeatedly. The structure of ADH was assessed using fluorescence emission spectra probed by 1-anilinonaphthalene-8-sulfonate over the repeated freeze–thaw cycles. The activity was monitored at 340 nm spectrophotometrically. Fluorescence data and activity clearly indicated that ADH without IBP was freeze-labile. However, ADH maintained about 70% residual activity after five repeated cycles at a minimal concentration of 0.1 mg mL-1 of high IRI-active LeIBP, but only 50% activity at 4 mg mL−1 of low active FfIBP. These results showed that the protection of proteins from freeze–thaw stress by IBPs is proportional to their IRI activity.

scholarly journals Enhancing the Freeze–Thaw Durability of Concrete through Ice Recrystallization Inhibition by Poly(vinyl alcohol)

ACS Omega ◽  
2020 ◽  
Vol 5 (22) ◽  
pp. 12825-12831
Author(s):  
Zhengyao Qu ◽  
Shuaiqi Guo ◽  
Christian C. M. Sproncken ◽  
Romà Surís-Valls ◽  
Qingliang Yu ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Ice Recrystallization Inhibition ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Recrystallization Inhibition ◽  
Durability Of Concrete

scholarly journals Freeze-Thaw Tolerance and Clues to the Winter Survival of a Soil Community

2006 ◽  
Vol 72 (3) ◽  
pp. 1784-1792 ◽  
Author(s):  
Virginia K. Walker ◽  
Gerald R. Palmer ◽  
Gerrit Voordouw
Keyword(s):  
Polar Regions ◽  
Pseudomonas Chlororaphis ◽  
Gene Fragment ◽  
Freezing And Thawing ◽  
Soil Community ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Subzero Temperatures ◽  
Single Colony ◽  
16S Rna

ABSTRACT Although efforts have been made to sample microorganisms from polar regions and to investigate a few of the properties that facilitate survival at freezing or subzero temperatures, soil communities that overwinter in areas exposed to alternate freezing and thawing caused by Foehn or Chinook winds have been largely overlooked. We designed and constructed a cryocycler to automatically subject soil cultures to alternating freeze-thaw cycles. After 48 freeze-thaw cycles, control Escherichia coli and Pseudomonas chlororaphis isolates were no longer viable. Mixed cultures derived from soil samples collected from a Chinook zone showed that the population complexity and viability were reduced after 48 cycles. However, when bacteria that were still viable after the freeze-thaw treatments were used to obtain selected cultures, these cultures proved to be >1,000-fold more freeze-thaw tolerant than the original consortium. Single-colony isolates obtained from survivors after an additional 48 freeze-thaw cycles were putatively identified by 16S RNA gene fragment sequencing. Five different genera were recognized, and one of the cultures, Chryseobacterium sp. strain C14, inhibited ice recrystallization, a property characteristic of antifreeze proteins that prevents the growth of large, potentially damaging ice crystals at temperatures close to the melting temperature. This strain was also notable since cell-free medium derived from cultures of it appeared to enhance the multiple freeze-thaw survival of another isolate, Enterococcus sp. strain C8. The results of this study and the development of a cryocycler should allow further investigations into the biochemical and soil community adaptations to the rigors of a Chinook environment.

scholarly journals Enhancing the Freeze-Thaw Durability of Concrete Through Ice Recrystallization Inhibition by Poly(vinyl Alcohol)

2019 ◽  
Author(s):  
Zhengyao Qu ◽  
Shuaiqi Guo ◽  
Christian C. M. Sproncken ◽  
Romà Surís-Valls ◽  
qingliang yu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Vinyl Alcohol ◽  
Cement Hydration ◽  
Poly Vinyl Alcohol ◽  
Seasonal Temperature ◽  
Ice Recrystallization Inhibition ◽  
Ice Recrystallization ◽  
Freeze Thaw ◽  
Recrystallization Inhibition ◽  
Durability Of Concrete

Frost weathering of porous materials caused by seasonal temperature changes is a major source of damage to the world’s infrastructure and cultural heritage. Here we investigate poly(vinyl alcohol) (PVA) addition as a means to enhance the freeze-thaw durability of concrete without compromising its structural or mechanical integrity. We evaluate the ice recrystallization inhibition activity of PVA in a cementitious environment and the impact of PVA on key structural and mechanical properties, such as cement hydration (products), microstructure, strength, as well as freeze‑thaw resistance. We find that a low amount of PVA significantly reduces the surface scaling of concrete and displays excellent ice recrystallization inhibition in the saturated Ca(OH)<sub>2 </sub>solution which has a similar pH value as cement pore solution, while it does not affect cement hydration, microstructure, nor its mechanical properties. These findings contribute to new insights on freeze-thaw damage mechanism and more importantly we disclose a new direction for the design of concrete with excellent freeze‑thaw resistance.

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