Black Cumin Pressing Waste Material as a Functional Additive for Starch Bread

The aim of the study was to determine the effect of the addition of black cumin (Nigella sativa L.) pressing waste (BCW) and black cumin seeds (BCS) on the properties of starch bread. The control bread was prepared from wheat starch (100%) with a gluten-free certificate, plantain husk (5%), and guar gum (2%). BCS and BCW were added between 0 and 10% of wheat starch. We determined the physicochemical properties, color, texture, and sensory properties of the prepared bread. Gas chromatography–mass spectrometry (GC–MS) analysis was performed to detect the phenolic compounds in the bread. The bread prepared with 6% BCS and 4% BCW had a significantly higher volume than the starch control bread did. Sensory analysis (taste) showed that BCS and BCW could be added up to 4% and 8%, respectively. The addition of BCS and BCW reduced the brightness of the crumb. A significant decrease in the L * index of the crumb was observed from 50.9 for the control bread to 34.1 and 34.0 for bread with 10% BCS and BCW, respectively. The addition of BCS and BCW decreased the hardness, elasticity, and chewiness of the starch bread crumb. Starch bread enriched with BCS and BCW was characterized by a higher content of 2-hydroxybenzoic acid, 2-hydroxyphenyl acetic acid, and 4-hydroxyphenyl acetic acid.

Evaluation of the rheological properties of the dough and the characteristics of the bread with the addition of purple potato

The aim of this study was to determine the rheological characteristics of the dough at the addition of purple potato powder in different quantities (0-100 g). The wheat flour types 480 and 1250 were used. The rheological characteristics of the dough were determined with the help of Chopin Alveograph analyzing the tensile strength. The rheological measurements were made with the HAAKE RheoWin Mars 40 rheometer and the dough’s viscoelastic modulus was analyzed at the frequency of 1-20 Hz. The bread samples were analyzed in terms of volume, porosity, color and texture. The textural parameters determined with the help of the texturometer were the elasticity, adhesiveness and stickiness of the bread crumb. It has been found that the addition of purple potato dough does not adversely alter the rheological properties of the dough. The elasticity and stickiness of the bread core increases with the increase of potato addition in the case of whole-grain flour. The color of the bread intensifies as the dose of purple potato powder increases.

Acid Stable α-Amylase Supplementation in Sourdough Enhanced Lactic Acid Bacterial Performance and the Quality of Bread

The effects of an acid-stable α-amylase AmyE from Aspergillus niger on the growth and fermentative ability of sourdough-fermenting Lactobacillus plantarum (LP) were studied and the accompanying changes in the dough rheological properties, specific volume of bread, crumb texture, and the microstructure of dough and bread were analyzed. Addition of acid-stable α-amylase AmyE in the sourdough at a concentration of 15 U/g significantly increased population of LP to 11.61 log CFU/g and accelerated acidification process compared to the commercial α-amylase supplemented sourdough and the control (non-amylase treated) during 24 h fermentation. Tensile test showed that adding AmyE induced an increase in maximal dough resistance to stretching and a decrease in extended distance. From scanning electron microscopy (SEM) images, the microstructure of dough displayed an advanced stage of granules swelling and much more deformation with increasing levels of α-amylase. The microstructure of breads prepared with AmyE was more homogeneous with increasing number of gas cells and resulted in improvement of specific volume and softness of bread crumbs. Given the aforementioned benefits of AmyE for lactic acid bacterial performance and bread quality, this acid-stable α-amylase AmyE has the potential as an improved enzyme additive for sourdough bread production.

Use of chromium-enriched yeast in the manufacture of French bread

Bread enriched with chromium has been associated with improved glycemic control. The purpose of this study was the production of a bread with chromium (Cr III) -enriched yeast which maintained overall bread quality. A formulation of French bread was prepared using an inactive yeast enriched with Cr III for comparison against a control bread made with standard yeast. Yeast fermentation power and bread color and texture were evaluated. There were no differences in the color (crumb and crust) or the firmness of the bread crumb, despite a significant difference (p < 0.05) in the yellow tonality (b*) of the bread crumb. Regarding cohesiveness, samples present similar values but a significant difference (p < 0.05) was detected between control and chromium breads. The average value of chromium in bread after processing was 1.717 μg/g in dry samples. This study concludes that it is possible to produce French bread with chromium enriched - yeast without changing the technological process or dramatically affecting its color and texture characteristics.

Impact of exogenous maltogenic α-amylase and maltotetraogenic amylase on sugar release in wheat bread

The use of exogenous maltogenic α-amylases or maltotetraogenic amylases of bacterial origin is common in wheat bread production, mainly as antistaling agents to retard crumb firming. To study the impact of maltogenic α-amylase and maltotetraogenic amylase on straight dough wheat bread, we performed a discovery-driven proteomics approach with commercial enzyme preparations and identified the maltotetraogenic amylase P22963 from Pelomonas saccharophila and the maltogenic α-amylase P19531 from Geobacillus stearothermophilus, respectively, as being responsible for the amylolytic activity. Quantitation of mono-, di- and oligosaccharides and residual amylase activity in bread crumb during storage for up to 96 h clarified the different effects of residual amylase activity on the sugar composition. Compared to the control, the application of maltogenic α-amylase led to an increased content of maltose and especially higher maltooligosaccharides during storage. Residual amylase activity was detectable in the breads containing maltogenic α-amylase, whereas maltotetraogenic amylase only had a very low residual activity. Despite the residual amylase activities and changes in sugar composition detected in bread crumb, our results do not allow a definite evaluation of a potential technological function in the final product. Rather, our study contributes to a fundamental understanding of the relation between the specific amylases applied, their residual activity and the resulting changes in the saccharide composition of wheat bread during storage.

Development of optimized substitution ratio for wheatcassava-african yam bean flour composite for Nigerian bread industries

An optimization study of the mix ratio for substitution of Wheat flour with Cassava and African Yam Bean flours (AYB) was carried out and reported in this paper. The aim was to obtain a mix ratio that would optimise selected physical properties of the bread. Wheat flour was substituted with Cassava and African Yam Bean flours at different levels: 80% to 100% of wheat, 0% to 10% of cassava flour and 0% to 10% for AYB flour. The experiment was conducted in mixture design which was generated and analysed by Design-Expert Software 11 version. The Composite dough was prepared in different mix ratios according to the design matrix and subsequentlybaked under the same conditions and analysed for the following loaf quality attributes: Loaf Specific Volume, Bread Crumb Hardness and Crumb Colour Index as response variables. The objective functions were to maximize Loaf Specific Volume, minimize Wheat flour, Bread Crumb Hardness and Crumb Colour Index to obtain the most suitable substitution ratio acceptable to consumers. Predictive models for the response variables were developed with the coefficient of determination (R2 ) of 0.991 for Loaf Specific Volume (LSV) while that of Bread Crumb Hardness (BCH) and Crumb Colour Index (CCI) were 0.834 and 0.895 respectively at 95% confidence interval (CI).The predicted optimal substitution ratio was obtained as follows: 88% Wheat flour, 10% Cassava flour, and 2% AYB flour. At this formulation, the predicted Loaf Specific Volume was 2.11cm3 /g, Bread Crumb Hardness was 25.12N, and Crumb Colour Index was 18.88.The study shows that addition of 2% of AYB flour in the formulation would help to optimise the LSV, BCH and the CCI of the Wheat-Cassava flour bread at the mix ratio of 88:10. Application of the results of this study in bread industries will reduce the cost of bread in Nigeria, which is influenced by the rising cost of imported wheat. This is a significant development because wheat flour was the sole baking flour in Nigeria before wheat substitution initiative. Keywords: Bread, wheat, Cassava, African Yam Bean, Flour, Dough, Loaf Specific Volume, Crumb Hardness, Crumb Colour Index

LF NMR spectroscopy analysis of water dynamics and texture of Gluten-Free bread with cricket powder during storage

The paper presents the effect of replacing starch (at 2%, 6% and 10%) with cricket powder (CP) on the water behavior studied by the 1H NMR method, as well as the texture of gluten-free bread during 6-day storage. It was noticed that the bread crumb containing CP has lower water transport rate than the control bread crumb, while concluding that 2% CP stabilizes water transport throughout the entire staling time range. The NMR analyzes showed that the initial T21 values are the higher, the more starch has been replaced with the CP, however, after 6 days of storage, all tested samples are characterized by similar values of the T21 parameter. A decrease in long component of spin-spin relaxation time T22 during storage was also observed. It has been noted that the replacement of starch to 2% and 6% CP causes an increase in the molecular dynamics of water. The less starch present, the greater the potential for bulk molecules to move. The observed changes at the molecular level resulted in macroscopic changes in the texture of the bread. After analyzing the hardness parameter of the tested breads, it was found that on the day of baking, bread without the addition of CP had significantly higher values of this parameter than breads with CP. For the sample without CP, the highest increase in total hardness change (123.93%) was noted during storage, which indicates the fastest texture change process. Based on the results obtained, it can be concluded that the use of cricket powder to enrich gluten-free bread can not only improve the nutritional value, but also effectively delay the process of bread staling.

Impact of exogenous α-amylases on sugar formation in straight dough wheat bread

The use of bacterial or fungal α-amylases is common in wheat bread production to improve several quality-related parameters such as loaf volume, crust color or staling behavior. To study the impact of exogenous α-amylases on straight dough wheat bread, we quantitated mono-, di- and oligosaccharides and residual α-amylase activity in bread crumb during storage for up to 96 h. Discovery-driven proteomics of the five α-amylase preparations studied showed that only a few different amylases per preparation were responsible for the hydrolytic effect. Compared to the control, the supplementation with α-amylase from Bacillus amyloliquefaciens in wheat dough preparation led to major changes in the sugar composition of bread crumb during storage with the formation of oligosaccharides like maltopentaose, maltohexaose, maltoheptaose, and maltooctaose. A residual activity corresponding to 4.0% of the applied activity was determined in the breads prepared with α-amylase from B. amyloliquefaciens, but no residual activity was detected for any of the other fungal or bacterial α-amylases from Aspergillus oryzae or Thermoactinomyces vulgaris. Whether the detected residual activity is related to the characteristics of bread staling or bread crumb properties must be clarified in further studies.