scholarly journals The Persistence of Staphylococcus aureus in Pressure Ulcers: A Colonising Role

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1883
Author(s):  
Martin Fayolle ◽  
Madjid Morsli ◽  
Anthony Gelis ◽  
Marion Chateauraynaud ◽  
Alex Yahiaoui-Martinez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Pressure Ulcers ◽  
Major Complication ◽  
Ring Test ◽  
Aureus Strain ◽  
Whole Genome ◽  
Significant Difference ◽  
Growth Profiles ◽  
Polymicrobial Communities

Decubitus pressure ulcers (PU) are a major complication of immobilised patients. Staphylococcus aureus is one of the most frequently detected microorganisms in PU samples; however, its persistence and role in the evolution of these wounds is unknown. In this study, we analysed S. aureus strains isolated from PU biopsies at inclusion and day 28. Eleven S. aureus (21.1%) were detected in 52 patients at inclusion. Only six PUs (11.5%) continued to harbour this bacterium at day 28. Using a whole genome sequencing approach (Miseq®, Illumina), we confirmed that these six S. aureus samples isolated at D28 were the same strain as that isolated at inclusion, with less than 83 bp difference. Phenotypical studies evaluating the growth profiles (Infinite M Mano, Tecan®) and biofilm formation (Biofilm Ring Test®) did not detect any significant difference in the fitness of the pairs of S. aureus. However, using the Caenorhabditis elegans killing assay, a clear decrease of virulence was observed between strains isolated at D28 compared with those isolated at inclusion, regardless of the clinical evolution of the PU. Moreover, all strains at inclusion were less virulent than a control S. aureus strain, i.e., NSA739. An analysis of polymicrobial communities of PU (by metabarcoding approach), in which S. aureus persisted, demonstrated no impact of Staphylococcus genus on PU evolution. Our study suggested that S. aureus presented a colonising profile on PU with no influence on wound evolution.

scholarly journals Whole-Genome Sequence for Methicillin-Resistant Staphylococcus aureus Strain ATCC BAA-1680

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Luke T. Daum ◽  
Violet V. Bumah ◽  
Daniela S. Masson-Meyers ◽  
Manjeet Khubbar ◽  
John D. Rodriguez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome ◽  
Strain Atcc ◽  
Methicillin Resistant

scholarly journals Biofilm Formation in Methicillin-Resistant Staphylococcus aureus Isolated in Cystic Fibrosis Patients Is Strain-Dependent and Differentially Influenced by Antibiotics

2021 ◽  
Vol 12 ◽  
Author(s):  
Agathe Boudet ◽  
Pauline Sorlin ◽  
Cassandra Pouget ◽  
Raphaël Chiron ◽  
Jean-Philippe Lavigne ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
Ring Test ◽  
Methicillin Resistant ◽  
Lung Dysfunction ◽  
Chronic Colonization ◽  
Strain Dependent

Cystic fibrosis (CF) is a genetic disease with lung abnormalities making patients particularly predisposed to pulmonary infections. Staphylococcus aureus is the most frequently identified pathogen, and multidrug-resistant strains (MRSA, methicillin-resistant S. aureus) have been associated with more severe lung dysfunction leading to eradication recommendations. Diverse bacterial traits and adaptive skills, including biofilm formation, may, however, make antimicrobial therapy challenging. In this context, we compared the ability of a collection of genotyped MRSA isolates from CF patients to form biofilm with and without antibiotics (ceftaroline, ceftobiprole, linezolid, trimethoprim, and rifampicin). Our study used standardized approaches not previously applied to CF MRSA, the BioFilm Ring test® (BRT®), the Antibiofilmogram®, and the BioFlux™ 200 system which were adapted for use with the artificial sputum medium (ASM) mimicking conditions more relevant to the CF lung. We included 63 strains of 10 multilocus sequence types (STs) isolated from 35 CF patients, 16 of whom had chronic colonization. The BRT® showed that 27% of the strains isolated in 37% of the patients were strong biofilm producers. The Antibiofilmogram® performed on these strains showed that broad-spectrum cephalosporins had the lowest minimum biofilm inhibitory concentrations (bMIC) on a majority of strains. A focus on four chronically colonized patients with inclusion of successively isolated strains showed that ceftaroline, ceftobiprole, and/or linezolid bMICs may remain below the resistance thresholds over time. Studying the dynamics of biofilm formation by strains isolated 3years apart in one of these patients using BioFlux™ 200 showed that inhibition of biofilm formation was observed for up to 36h of exposure to bMIC and ceftaroline and ceftobiprole had a significantly greater effect than linezolid. This study has brought new insights into the behavior of CF MRSA which has been little studied for its ability to form biofilm. Biofilm formation is a common characteristic of prevalent MRSA clones in CF. Early biofilm formation was strain-dependent, even within a sample, and not only observed during chronic colonization. Ceftaroline and ceftobiprole showed a remarkable activity with a long-lasting inhibitory effect on biofilm formation and a conserved activity on certain strains adapted to the CF lung environment after years of colonization.

scholarly journals Whole-Genome Sequence of the Ancestral Animal-Borne ST398 Staphylococcus aureus Strain S123

2013 ◽  
Vol 1 (5) ◽  
Author(s):  
N. van der Mee-Marquet ◽  
D. Hernandez ◽  
X. Bertrand ◽  
R. Quentin ◽  
A.-R. Corvaglia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome

scholarly journals Whole-genome sequence data and analysis of a Staphylococcus aureus strain SJTUF_J27 isolated from seaweed

Data in Brief ◽  
2018 ◽  
Vol 20 ◽  
pp. 894-898
Author(s):  
Yanping Xie ◽  
Yiping He ◽  
Sandeep Ghatak ◽  
Peter Irwin ◽  
Xianghe Yan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Sequence Data ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome ◽  
Genome Sequence Data

scholarly journals Susceptibility of Ocular Staphylococcus aureus to Antibiotics and Multipurpose Disinfecting Solutions

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1203
Author(s):  
Madeeha Afzal ◽  
Ajay Kumar Vijay ◽  
Fiona Stapleton ◽  
Mark D. P. Willcox
Keyword(s):  
Staphylococcus Aureus ◽  
Contact Lens ◽  
Ocular Surface ◽  
Polymyxin B ◽  
Multidrug Resistant ◽  
Aureus Strain ◽  
Microbial Keratitis ◽  
Significant Difference ◽  
Different Types ◽  
The Usa

Staphylococcus aureus is a frequent cause of ocular surface infections worldwide. Of these surface infections, those involving the cornea (microbial keratitis) are most sight-threatening. S. aureus can also cause conjunctivitis and contact lens-related non-infectious corneal infiltrative events (niCIE). The aim of this study was to determine the rates of resistance of S. aureus isolates to antibiotics and disinfecting solutions from these different ocular surface conditions. In total, 63 S. aureus strains from the USA and Australia were evaluated; 14 were from niCIE, 26 from conjunctivitis, and 23 from microbial keratitis (MK). The minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of all the strains to ciprofloxacin, ceftazidime, oxacillin, gentamicin, vancomycin, chloramphenicol, azithromycin, and polymyxin B were determined. The MIC and MBC of the niCIE strains to contact lens multipurpose disinfectant solutions (MPDSs) was determined. All isolates were susceptible to vancomycin (100%). The susceptibility to other antibiotics decreased in the following order: gentamicin (98%), chloramphenicol (76%), oxacillin (74%), ciprofloxacin (46%), ceftazidime (11%), azithromycin (8%), and polymyxin B (8%). In total, 87% of all the isolates were multidrug resistant and 17% of the isolates from microbial keratitis were extensively drug resistant. The microbial keratitis strains from Australia were usually susceptible to ciprofloxacin (57% vs. 11%; p = 0.04) and oxacillin (93% vs. 11%; p = 0.02) compared to microbial keratitis isolates from the USA. Microbial keratitis isolates from the USA were less susceptible (55%) to chloramphenicol compared to conjunctivitis strains (95%; p = 0.01). Similarly, 75% of conjunctivitis strains from Australia were susceptible to chloramphenicol compared to 14% of microbial keratitis strains (p = 0.04). Most (93%) strains isolated from contact lens wearers were killed in 100% MPDS, except S. aureus 27. OPTI-FREE PureMoist was the most active MPDS against all strains with 35% of strains having an MIC ≤ 11.36%. There was a significant difference in susceptibility between OPTI-FREE PureMoist and Biotrue (p = 0.02). S. aureus non-infectious CIE strains were more susceptible to antibiotics than conjunctivitis strains and conjunctivitis strains were more susceptible than microbial keratitis strains. Microbial keratitis strains from Australia (isolated between 2006 and 2018) were more susceptible to antibiotics in comparison with microbial keratitis strains from the USA (isolated in 2004). Most of the strains were multidrug-resistant. There was variability in the susceptibility of contact lens isolates to MPDSs with one S. aureus strain, S. aureus 27, isolated from niCIE, in Australia in 1997 being highly resistant to all four MPDSs and three different types of antibiotics. Knowledge of the rates of resistance to antibiotics in different conditions and regions could help guide treatment of these diseases.

Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef

2021 ◽  
Author(s):  
F. Özdemir ◽  
S. Arslan ◽  
C. B. Eken
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Ground Beef ◽  
Microtiter Plate ◽  
Type I ◽  
Type Ii ◽  
Crystal Violet Assay ◽  
Biofilm Production ◽  
Significant Difference ◽  
Mtt Assays

AbstractA total of 46 Staphylococcus aureus isolates from fish and ground beef were tested for the agr types, icaABCD genes, and biofilm formation at 12, 25 and 37 °C by the microtiter plate and the MTT assays. All isolates were positive for the icaABD genes, while 97.8% were positive for the icaC. All isolates produced biofilms at 37 and 25 °C, but 93.5% of them were also biofilm producers at 12 °C. There was no significant difference in biofilm formation between 25 and 37 °C using the crystal violet assay (P > 0.05). However, statistically significant differences were detected between 12 and 25 °C as well as 12 and 37 °C (P < 0.05). All isolates were significantly different in biofilm production by the MTT assay at all tested temperatures. Furthermore, a relationship between the presence of the icaABCD genes and biofilm formation was observed. The agr type I was the most prevalent (54.4%) among the isolates, followed by agr type II (41.3%) and agr type III (9.6%). In this study, the S. aureus isolates exhibited biofilm formation ability responsible for persistence of bacteria in foods, which may lead to food spoilage and human health problems.

scholarly journals Whole-Genome Sequence of Staphylococcus aureus Strain LCT-SA112

2012 ◽  
Vol 194 (15) ◽  
pp. 4124-4124 ◽  
Author(s):  
J. Wang ◽  
Y. Liu ◽  
D. Wan ◽  
X. Fang ◽  
T. Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome
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