scholarly journals Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant

2019 ◽  
Vol 8 (4) ◽  
pp. 18 ◽  
Author(s):  
Hanifeh Seyed Hajizadeh ◽  
Bahram Heidari ◽  
Giovanni Bertoldo ◽  
Maria Cristina Della Lucia ◽  
Francesco Magro ◽  
...  

Leonardite-based biostimulants are a large class of compounds, including humic acid substances. Foliar application of biostimulants at field level improves plant growth, yield and quality through metabolic changes and stimulation of plant proton pumps. The present study aimed at identifying optimum dosage of BLACKJAK, a humic acid-based substance, which is able to modify genes involved in sugar beet growth. Thirty-three genes belonging to various biochemical pathway categories were tested in leaves of treated sugar beet (Beta vulgaris L.) samples to assess gene expression profiling in response to BLACKJAK. Seedlings of a diploid and multigerm variety were grown in plastic pots and sprayed with two dilutions of BLACKJAK (dilution 1:500–1.0 mg C L−1 and dilution 1:1000–0.5 mg C L−1). Leaf samples were collected after 24, 48, and 72 h treatment with BLACKJAK for each dilution. RNA was extracted and the quantification of gene expression was performed while using an OpenArray platform. Results of analysis of variance demonstrated that, 15 genes out of a total of 33 genes tested with OpenArray qPCR were significantly affected by treatment and exposure time. Analysis for annotation of gene products and pathways revealed that genes belonging to the mitochondrial respiratory pathways, nitrogen and hormone metabolisms, and nutrient uptake were up-regulated in the BLACKJAK treated samples. Among the up-regulated genes, Bv_PHT2;1 and Bv_GLN1 expression exerted a 2-fold change in 1:1000 and 1:500 BLACKJAK concentrations. Overall, the gene expression data in the BLACKJAK treated leaves demonstrated the induction of plant growth–related genes that were contributed almost to amino acid and nitrogen metabolism, plant defense system, and plant growth.

2020 ◽  
Vol 43 (15) ◽  
pp. 2275-2285
Author(s):  
R. M. Y. Zewail ◽  
I. S. El-Gmal ◽  
Botir Khaitov ◽  
Heba S. A. El-Desouky

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 137
Author(s):  
Ahmed A. A. Leilah ◽  
Naeem Khan

Two field trials were conducted during the 2014/2015 and 2015/2016 seasons at Aweesh Al-Hagar Village, center of Mansoura, Dakahlia Governorate, Egypt. A split-split-plot design with four replicates was used. The main plots were assigned three nitrogen fertilizer levels, i.e., 165, 220, and 275 kg/ha. The sub-plots were restricted to four gibberellic acid (GA3) concentrations, i.e., 0, 80, 160, and 240 mg/L, and the sub-sub plots received GA3 application twice, i.e., 60 and 120 days after planting (DAP). The results showed that both root length and diameter, root and foliage fresh weights/plant, and root and foliage yields/ha increased with the incremental level of nitrogen and/or GA3 concentration. Foliar application of GA3 and N-fertilizers also significantly decreased quality parameters including sucrose and total soluble solid (TSS) percentages. Early application of GA3 (60 DAP) had an active role on sugar beet growth, yield, and quality compared with spraying at 120 DAP. Generally, fertilizing sugar beet with 275 kg N/ha or spraying GA3 with a concentration of 160 mg/L at 60 DAP is the recommended treatment for raising sugar yield under the ecological circumstances of this research.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 675
Author(s):  
Maha M.E. Ali ◽  
Spyridon A. Petropoulos ◽  
Daila AbdelFattah Fattah H. Selim ◽  
Mohssen Elbagory ◽  
Maha Othman ◽  
...  

The present work evaluated the effect of soil (S) and foliage (F) applied potassium on the growth, yield, and quality of potato plants. Potassium was added in soil at the recommended rate for all the treatments combining mineral fertilizers with K-feldspar and biofertilizers, while foliar spraying included the application of potassium citrate (PC), potassium silicate (PS), and monopotassium phosphate (MP). The obtained results showed that plant height was highest following treatment with 100% mineral potassium fertilizer under the foliar application of MP, while the content of P, K, and total carbohydrates in leaves also increased with the same fertilization treatment. On the other hand, the highest values for number of stems and fresh and dry weight per plant, as well as the highest nitrogen content in leaves, were obtained after the addition of mineral potassium fertilizer and the foliar spraying of PC, regardless of the growing season. Yield parameters were positively affected by the combination of mineral potassium fertilizers (100% or 80% K2SO4 + 20% K-feldspar + biofertilizer) and the foliar spraying of MP, while the total nitrogen, protein, amino acids, potassium, phosphorus, and starch content of tubers were positively affected by the same mineral fertilizer treatments combined with foliar spraying of MP or CP. In conclusion, the application of mineral potassium fertilizer with foliar spraying of MP or CP increased most of the plant growth- and tuber chemical composition-related parameters. These results highlight the importance of potassium fertilizer regimes for achieving high tuber yields and improving the quality of tubers in a sustainable and cost-effective manner.


Author(s):  
С. В. Філоненко

Регулятори росту і розвитку рослин стають важ-ливою складовою сучасних технологій вирощування сільськогосподарських культур. Автором вивчено закономірності впливу позакореневого внесення регу-лятора росту «Марс-1» на формування врожайності та якості коренеплодів буряка цукрового гібриду Іва-нівсько-Веселоподільський ЧС 84. Рекомендовано застосовувати «Марс-1» двічі: перший раз – у фазі чотирьох пар справжніх листків, другий – перед змиканням листя у міжряддях. Доза для кожного внесення – 0,8 л/га. Regulators of plant growth and development are an important part of modern technologies of agricultural crops. The author studied the regularities of foliar application of growth regulator “Mars -1” on the formation of yield and quality of sugar beet roots hybrid Ivanivsko-Veselopodilskyy - 84. It is recommended to apply “Mars-1” twice: the first time – in the phase of four pairs of true leaves, in the second – before closing the leaves between rows. The dose for each entry – 0,8 l / ha.


Author(s):  
Saied Kamel Mohamed Abd El-Naby ◽  
Amr Abdelkhalek Ahmed Mohamed ◽  
Yahia Ibrahim Mohamed El-Naggar

A field experiment was carried out during 2017–2018 in the Horticulture Research Station farm in El Kanater El Khayreia, El Kalubeia Governorate, Egypt, to find out the response of foliar application of melatonin, GA3 and NAA on vegetative and fruit attributes of ‘Canino’ apricot. Different plant growth regulators influ- enced all variable parameters related to vegetative growth and fruit. Results revealed that between all growth regulators, the foliar application of melatonin following by GA3 there was the highest vegetative growth as compared to other treatments, while GA3 gave the highest yield following by melatonin as compared to other treatments. Treatment with NAA improved vegetative growth, yield and fruit quality to a lesser extent as compared to the control.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 806
Author(s):  
Ali A. A. Mekdad ◽  
Mostafa M. Rady ◽  
Esmat F. Ali ◽  
Fahmy A. S. Hassan

Field trials for two seasons (2018/2019 and 2019/2020) were conducted to investigate the influence of the addition of three levels of potassium (K) (K1 = 60, K2 = 120, and K3 = 180 kg K2O ha−1) and/or sulfur (S) (S1 = 175, S2 = 350, and S3 = 525 kg CaSO4 ha−1) to the soil, as well as the sowing date (the 1st of September, D1; or the 1st of October, D2) on the potential improvement of physiology, growth, and yield, as well as the quality characteristics of sugar beet yield under soil salinity conditions. With three replicates specified for each treatment, each trial was planned according to a split-split plot in a randomized complete block design. The results revealed that early sowing (D1) led to significant improvements in all traits of plant physiology and growth, in addition to root, top, and biological yields and their quality, gross and pure sugar, and K- and S-use efficiencies based on root yield (R-KUE and R-SUE). The K3 level (180 kg K2O ha−1) positively affected the traits of plant physiology, growth, yield and quality, and R-SUE, and reduced the attributes of impurities, impurity index, and R-KUE. Additionally, the S3 level (525 kg CaSO4 ha−1) affirmatively affected plant physiology, growth, yield and quality traits, and R-KUE, and decreased impurity traits, impurity index, and R-SUE. The interaction of D1 × K3 × S3 maximized the yield of roots (104–105 ton ha−1) and pure sugar (21–22 ton ha−1). Path coefficient analysis showed that root yield and pure sugar content had positive direct effects with 0.62 and 0.65, and 0.38 and 0.38 in both studied seasons, respectively, on pure sugar yield. Significant (p ≤ 0.01) positive correlations were found between pure sugar yield and root yield (r = 0.966 ** and 0.958 **). The study results recommend the use of the integrative D1 × K3 × S3 treatment for sugar beet to obtain maximum yields and qualities under salt stress (e.g., 8.96 dS m−1) in dry environments.


2021 ◽  
Vol 42 (2) ◽  
pp. 326-331
Author(s):  
M. Singh ◽  
◽  
K.S. Sandhu ◽  

Aim: To determine the impact of soil and foliar application of ZnSO4.7H2O at late stages of wheat as heading initiation (5% ear formation), 100% heading (complete ear formation) and heading initiation and 100% heading along with recommended dose of fertilizer on growth, yield and quality of zero till wheat. Methodology: The field experiment was conducted on zero till wheat. The treatments consisted of control (no Zn), soil application of 12.5, 25, 37.5, 50 kg ha-1 ZnSO4.7H2O and foliar application of 0.5% Zn as one spray at heading initiation (5% ear formation), one spray at 100% heading (complete ear formation) and two sprays at heading initiation and 100% heading with recommended dose of fertilizer. These treatments were evaluated in RBD with three replications. Results: Soil application of 50, 37.5 and 25 kg Zn ha-1 with two foliar sprays of 0.5% at heading initiation (5% ear formation) and 100% heading (complete ear formation) stages gave significantly higher average grain and straw yield and Zn concentration in grain than other treatments, including control. Interpretation: Enhanced application of Zn as soil and foliar application ameliorates soil Zn deficiency and increases protein content in grains, which might influence the quality and yield of zero tilled wheat. Key words: Foliar spray, Grain yield, Wheat, Zinc


Author(s):  
Ashish Kumar Dubey ◽  
Devi Singh ◽  
Pranjal Singh Rajput ◽  
Yogesh Kumar ◽  
Ajay Kumar Verma ◽  
...  

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