scholarly journals Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria

2020 ◽  
Vol 17 (20) ◽  
pp. 7501
Author(s):  
Imane Lamraoui ◽  
Adel Eltoukhy ◽  
Junhuan Wang ◽  
Messaouda Lamraoui ◽  
Amer Ahmed ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Acid Ester ◽  
Phthalic Acid ◽  
Degradation Pathway ◽  
Rrna Gene ◽  
16S Rrna Gene Analysis ◽  
Phthalic Acid Ester ◽  
Wide Range ◽  
Ring Structures ◽  
Ethylhexyl Phthalate

Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems. Enterobacter spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones.

scholarly journals Preliminary study on diminution level of RNA/DNA ratio in tissue of Labeo rohita by exposure to some endocrine disrupting compounds (EDCs)

2016 ◽  
Vol 1 (1) ◽  
pp. 16-20
Author(s):  
Rita Verma ◽  
Atul K. Singh ◽  
Kamal Jaiswal
Keyword(s):  
Acid Ester ◽  
Phthalic Acid ◽  
Natural Waters ◽  
Labeo Rohita ◽  
Statistical Significance ◽  
Endocrine Disrupting Compounds ◽  
Phthalic Acid Ester ◽  
Endocrine Disrupting ◽  
Butyl Phthalate ◽  
Ethylhexyl Phthalate

Effects of EDCs particularly on RNA/DNA ratio are yet to be investigated to manage the effluents in natural waters. We investigated exposure effects of endocrine disrupting compounds (EDCs) phthalic acid ester (PAE) and hexachlorocyclohexane (HCH) on the RNA/DNA ratio in tissue of an Indian major carp Labeo rohita. Fish were exposed to pre-determined sublethal concentrations of phthalic acid ester (Di-methyl phthalate (DMP), di-butyl phthalate (DBP), and di-(2- ethylhexyl) phthalate (DEHP) and also HCH for determining the tissue RNA/DNA ratio after 30, 60 and 90 days of exposure in the doses of 0.2 mg L-1, 0.3 mg L-1, and 0.5 mg L-1 respectively. All these tested chemicals significantly (P0.05) inhibited RNA/DNA ratio. The ratio gradually significantly (P0.05) decreased after DEHP where it was 1.9±0.51 F1, 18=15.8 P=0.014 n=19; in case of DBP it was 1.92±0.62 F1, 20=6.5 P=0.012 n=19 and for HCH it was 0.94±0.21 F1, 18=18.08 P=0.0012 n=19 at treatments concentrations of 0.3 mg L-1 and 0.5 mg L-1, compared to control (2.9±0.2) after 90 days. However, there was no statistical significance (P0.05) in RNA/DNA ratio after the DMP (F1, 20=2.4 P=0.15n=21) treatment.

A new phthalic acid ester fromAjuga bracteosa

2006 ◽  
Vol 20 (6) ◽  
pp. 593-597 ◽  
Author(s):  
Narendra Singh ◽  
Umar Mahmood ◽  
V. K. Kaul ◽  
Leopold Jirovetz
Keyword(s):  
Acid Ester ◽  
Phthalic Acid ◽  
Phthalic Acid Ester

scholarly journals Integrated multi-omics investigations reveal the key role of synergistic microbial networks in removing plasticizer di-(2-ethylhexyl) phthalate from estuarine sediments

2021 ◽  
Author(s):  
Sean Ting-Shyang Wei ◽  
Yi-Lung Chen ◽  
Yu-Wei Wu ◽  
Tien-Yu Wu ◽  
Yi-Li Lai ◽  
...  
Keyword(s):  
Phthalic Acid ◽  
Profile Analysis ◽  
Metabolite Profile ◽  
Degradation Pathway ◽  
Estuarine Sediments ◽  
Side Chain ◽  
Side Chains ◽  
Human Beings ◽  
Alkyl Side Chains ◽  
Ethylhexyl Phthalate

Di-(2-ethylhexyl) phthalate (DEHP) is the most widely used plasticizer worldwide with an annual global production of over eight million tons. Because of its improper disposal, endocrine-disrupting DEHP often accumulates in estuarine sediments in industrialized countries at sub-millimolar levels, resulting in adverse effects on both ecosystems and human beings. The microbial degraders and biodegradation pathways of DEHP in O2-limited estuarine sediments remain elusive. Here, we employed an integrated meta-omics approach to identify the DEHP degradation pathway and major degraders in this ecosystem. Estuarine sediments were treated with DEHP or its derived metabolites, o-phthalic acid and benzoic acid. The rate of DEHP degradation in denitrifying mesocosms was two times slower than that of o-phthalic acid, suggesting that side-chain hydrolysis of DEHP is the rate-limiting step of anaerobic DEHP degradation. On the basis of microbial community structures, functional gene expression, and metabolite profile analysis, we proposed that DEHP biodegradation in estuarine sediments is mainly achieved through synergistic networks between denitrifying proteobacteria. Acidovorax and Sedimenticola are the major degraders of DEHP side-chains; the resulting o-phthalic acid is mainly degraded by Aestuariibacter through the UbiD-dependent benzoyl-CoA pathway. We isolated and characterized Acidovorax sp. strain 210-6 and its extracellular hydrolase, which hydrolyzes both alkyl side-chains of DEHP. Interestingly, genes encoding DEHP/MEHP hydrolase and phthaloyl-CoA decarboxylase—key enzymes for side-chain hydrolysis and o-phthalic acid degradation, respectively—are flanked by transposases in these proteobacterial genomes, indicating that DEHP degradation capacity is likely transferred horizontally in microbial communities.

scholarly journals The Impact of Storage on Phthalic Acid Esters Concentrations in Yogurts Packed in Plastic Cups

2019 ◽  
Vol 67 (3) ◽  
pp. 689-693
Author(s):  
Marcela Jandlová ◽  
Vojtěch Kumbár ◽  
Alžbeta Jarošová ◽  
Markéta Stará ◽  
Roman Pytel ◽  
...  
Keyword(s):  
Acid Ester ◽  
Phthalic Acid ◽  
Limit Set ◽  
Phthalic Acid Esters ◽  
Bamboo Fibre ◽  
Phthalic Acid Ester ◽  
Significant Difference ◽  
The Impact ◽  
Acid Esters ◽  
Migration Limit

Phthalic acid esters are used as plastic softeners and also can be found in food packaging materials. European legislation defines specific migration limits of plastic additives for plastic materials that come into contact with food. This study monitors the phthalic acid ester concentrations in yogurts after manufacturing and then after a 3‑week storage. The studied yoghurts were natural yogurt with 1 % of chia flour, natural yogurt with 5 % of chia flour, natural yogurt with 1 % of bamboo fibre, natural yogurt with 5 % of bamboo fibre and natural yogurt. The analysed phthalic acid esters were dibutyl phthalate (DBP) and di‑(2‑ethylhexyl) phthalate (DEHP). The average phthalate concentrations in plastic cups were detected for DBP of 59.5 µg/g and for DEHP of 9.0 µg/g of the plastic material. Higher DBP concentrations than DEHP concentrations were also found in all studied yogurts. The average DBP concentrations in yogurts were detected from 1.8 µg/g up to 5.0 µg/g of the original matter and the average DEHP concentrations were determined from 0.5 µg/g up to 1.0 µg/g of the original matter. No statistically significant difference was found when comparing phthalic acid ester concentrations in yogurts immediately after production and after three weeks of storage. However, in our study in all cases of yogurts, the DBP concentrations were higher than the specific migration limit set by the legislation (0.3 mg/kg) and the DEHP concentrations were in all cases of yogurts lower than the specific migration limit set by the legislation (1.5 mg/kg).

Di-n-hexyl Phthalate (DHP), a Newly Identified Contaminant in Atlantic Herring (Clupea harengus harengus) and Atlantic Mackerel (Scomber scombrus)

1981 ◽  
Vol 38 (7) ◽  
pp. 856-859 ◽  
Author(s):  
Charles J. Musial ◽  
John F. Uthe ◽  
Gary R. Sirota ◽  
B. Garth Burns ◽  
Michael W. Gilgan ◽  
...  
Keyword(s):  
Acid Ester ◽  
Phthalic Acid ◽  
Clupea Harengus ◽  
Proton Nuclear Magnetic Resonance ◽  
Atlantic Herring ◽  
Isolation And Identification ◽  
Atlantic Mackerel ◽  
Scomber Scombrus ◽  
Phthalic Acid Ester ◽  
Clupea Harengus Harengus

We describe the isolation and identification of di-n-hexyl phthalate, a new marine pollutant found in Atlantic herring (Clupea harengus harengus) and Atlantic mackerel (Scomber scombrus). This compound was isolated from extracted lipids by a combination of gel permeation, high performance liquid and column chromatography, and selective precipitation of impurities. Conclusive proof of its structure was obtained from gas chromatographic–mass spectrometric analysis and proton nuclear magnetic resonance spectroscopy. The concentrations of di-n-hexyl phthalate (17–27 μg/g wet weight), which is not a major industrial phthalic acid ester, far exceeded those of dibutyl and di-2-ethylhexyl phthalates, the major industrial phthalic acid esters. Thus the possibility of selective bioaccumulation of DHP in herring and mackerel was indicated. This finding is important in view of the paucity of toxicological information concerning this compound.Key words: phthalic acid ester, di-n-hexyl phthalate (DHP), analysis, mackerel, herring, marine fish, pollution

scholarly journals Psychrobacillus soli sp. nov., capable of degrading oil, isolated from oil-contaminated soil

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3046-3052 ◽  
Author(s):  
Van Hong Thi Pham ◽  
Seung-Woo Jeong ◽  
Jaisoo Kim
Keyword(s):  
Contaminated Soil ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Period Analysis ◽  
Wide Range ◽  
Reference Strains

A novel, aerobic, psychrotolerant, Gram-stain-positive, endospore-forming strain, NHI-2T, was isolated from oil-contaminated soil near a gas station in Mongolia. This strain was characterized by motile rods and grew over a wide range of temperatures ( − 2 to 40 °C) with optimal growth at 28–30 °C. It tolerated salt concentrations of up to 7 % over a five-day incubation period. Analysis of 16S rRNA gene sequence indicated that strain NHI-2T belongs to the genus Psychrobacillus. Sequence similarity between NHI-2T and members of the genus Psychrobacillus with validly published names ranged from 97.83 to 98.18 %. DNA–DNA hybridization indicated less than 70 % relatedness to reference strains within the genus. The G+C content of the genomic DNA was 36 mol%. This strain contained MK-8 as a predominant isoprenoid menaquinone. NHI-2T had ornithine in the cell wall similar to reference strains of the genus Psychrobacillus. The major fatty acids present in NHI-2T were anteiso-C15 : 0 (51.0 %), iso-C15 : 0 (9.1 %) and anteiso-C17 : 0 (8.0 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. These data highlight that the phenotype of strain NHI-2T differs from that of related species in terms of chemotaxonomic properties and genotype characteristics. Therefore, this strain is proposed as a representative of a novel species, named Psychrobacillus soli. The type strain is NHI-2T ( = KEMB 9005-135T = KACC 18243T = NBRC 110600T).

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