scholarly journals Patulin Contamination of Citrus Fruits from Punjab and Northern Pakistan and Estimation of Associated Dietary Intake

2021 ◽  
Vol 18 (5) ◽  
pp. 2270
Author(s):  
Kinza Aslam ◽  
Shahzad Zafar Iqbal ◽  
Ahmad Faizal Abdull Razis ◽  
Sunusi Usman ◽  
Nada Basheir Ali
Keyword(s):  
Dietary Intake ◽  
Citrus Fruit ◽  
Natural Occurrence ◽  
The European Union ◽  
Northern Pakistan ◽  
Citrus Fruits ◽  
Uv Detector ◽  
Central Cities ◽  
Fruit Samples ◽  
Northern Cities

This research aims to assess the natural occurrence of patulin (PAT) in selected citrus fruits from central cities of Punjab and Pakistan’s northern cities. A total of 2970 fruit samples from 12 citrus cultivars were examined using liquid chromatography fitted with a UV detector. The detection limit (LOD) and quantification limit were 0.04 and 0.12 µg/kg, respectively. About 56% of samples of citrus fruits from Punjab’s central cities, Pakistan, were found to be contaminated with PAT, with values ranging from 0.12 to 1150 µg/kg in samples from central Punjab cities. Furthermore, 31.7% of samples of citrus fruits from northern cities of Pakistan were contaminated with PAT, with values ranging from 0.12 to 320 µg/kg. About 22.1% of citrus fruit samples had PAT levels greater than the suggested limits established by the European Union (EU). The dietary intake levels of PAT ranged from 0.10 to 1.11 µg/kg bw/day in the central cities of Punjab, Pakistan, and 0.13 to 1.93 µg/kg bw/day in the northern cities of Pakistan.

scholarly journals Variation of Patulin Levels in Citrus Fruits from Central Cities of Punjab and Northern Cities of Pakistan, and Estimation of Dietary Intake

2021 ◽  
Author(s):  
Shahzad Iqbal
Keyword(s):  
Dietary Intake ◽  
Limit Of Detection ◽  
Citrus Fruit ◽  
Natural Occurrence ◽  
The European Union ◽  
Citrus Fruits ◽  
Uv Detector ◽  
Central Cities ◽  
Fruit Samples ◽  
Northern Cities

The research aims to discover the natural occurrence of patulin (PAT) in selected citrus fruits from the central cities of Punjab and Pakistan's Northern cities. Total 2970 samples of twelve citrus fruits; kinnow, orange, grapefruits, bitter orange, mausami, red blood, pineapple, sweet orange, rough lime, sweet lime, kagzi lime and lemon were examined using liquid chromatography fitted with UV detector. The limit of detection (LOD) and quantification limit was 0.04 and 0.12 µg/kg, respectively. The results have shown that 56% of samples of citrus fruits from Punjab's central cities, Pakistan, were discovered to be infected from PAT. The elevated amounts of PAT ranging from 0.04 to 1150 µg/kg were found in citrus fruit samples from Multan cities. Furthermore, 31.7% of samples of citrus fruits from Northern cities of Pakistan were discovered to be infected with PAT, and the elevated amounts were found ranging from 0.04-320 µg/kg from Swat city. About 22.1% of samples of citrus fruits have levels of PAT greater than the suggested limits established by the European Union (EU). PAT's dietary intake levels ranged from 0.10-1.11 µg/kg bw/day from the central cities of Punjab, Pakistan, and 0.13-1.93 µg/kg bw/day were documented from Northern cities of Pakistan

scholarly journals Variation of Patulin Levels in Citrus Fruits From Central Cities of Punjab and Northern Cities of Pakistan, and Estimation of Dietary Intake

2021 ◽  
Author(s):  
Kinza Aslam ◽  
Shahzad Zafar Iqbal ◽  
Ahmad Faizal Abdull Razis ◽  
Sunusi Usman ◽  
Nada Basheir Ali
Keyword(s):  
Dietary Intake ◽  
Limit Of Detection ◽  
Citrus Fruit ◽  
Natural Occurrence ◽  
The European Union ◽  
Citrus Fruits ◽  
Uv Detector ◽  
Central Cities ◽  
Fruit Samples ◽  
Northern Cities

The research aims to discover the natural occurrence of patulin (PAT) in selected citrus fruits from the central cities of Punjab and Pakistan's Northern cities. Total 2970 samples of twelve citrus fruits; kinnow, orange, grapefruits, bitter orange, mausami, red blood, pineapple, sweet orange, rough lime, sweet lime, kagzi lime and lemon were examined using liquid chromatography fitted with UV detector. The limit of detection (LOD) and quantification limit was 0.04 and 0.12 µg/kg, respectively. The results have shown that 56% of samples of citrus fruits from Punjab's central cities, Pakistan, were discovered to be infected from PAT. The elevated amounts of PAT ranging from 0.04 to 1150 µg/kg were found in citrus fruit samples from Multan cities. Furthermore, 31.7% of samples of citrus fruits from Northern cities of Pakistan were discovered to be infected with PAT, and the elevated amounts were found ranging from 0.04-320 µg/kg from Swat city. About 22.1% of samples of citrus fruits have levels of PAT greater than the suggested limits established by the European Union (EU). PAT's dietary intake levels ranged from 0.10-1.11 µg/kg bw/day from the central cities of Punjab, Pakistan, and 0.13-1.93 µg/kg bw/day were documented from Northern cities of Pakistan.

scholarly journals The presence of pesticide residue in farmer market produce

2020 ◽  
Author(s):  
RuJun Zhou ◽  
BCIT School of Health Sciences, Environmental Health ◽  
Dale Chen ◽  
Hsin Kuo
Keyword(s):  
Pesticide Residue ◽  
Fruits And Vegetables ◽  
Solid Phase ◽  
Citrus Fruit ◽  
The Body ◽  
Farmers Markets ◽  
Citrus Fruits ◽  
Maximum Residue Level ◽  
Fruit Samples ◽  
Health Canada

Background: Thiabendazole is a pesticide that is mainly used after harvesting and directly applied to produce such as citrus fruits, apples, pears, bananas, mangos, corn, carrots and potatoes in the form of a spray or dip. The most common and most likely route of exposure to pesticide for the average person is through their diet. Studies have shown that the health risk of regular consumption of pesticide residue through produce is linked with disruption to various functions in the body, such as reproductive, developmental and hormone irregularity. The following study tests whether fruits and vegetables sold at farmers markets contain Thiabendazole and if they are below the acceptable Maximum Residue Limits (MRLs) set by Health Canada. Methods: A QuEChERS method and solid phase extraction was used to recover Thiabendazole from various fruits and vegetables. The gas chromatography was used to analyze all samples and a calibration curve was produced to identify the concentration of Thiabendazole. Results: Thiabendazole was detected in all of the citrus fruit samples, but was below detectable limits for all other fruits and vegetables. All Thiabendazole levels were below the Maximum Residue Level allowed by Health Canada. Conclusion: The various fruits and vegetables analyzed are all below the MRL, with only the citrus fruits having detectable concentrations. However, since the citrus fruits were imported, further studies are required on different pesticide compounds to determine if locally grown produce meet the MRL for other pesticide compounds.  

scholarly journals Application of a roller conveyor type plasma disinfection device with fungus-contaminated citrus fruits

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Akikazu Sakudo ◽  
Yoshihito Yagyu
Keyword(s):  
Treatment Time ◽  
Citrus Fruit ◽  
Viable Cell ◽  
Cell Number ◽  
Viable Cell Number ◽  
Citrus Fruits ◽  
Decimal Reduction Time ◽  
Gas Plasma ◽  
Plasma Device ◽  
Time Required

AbstractEfficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressure dielectric barrier discharge (APDBP) plasma on a set of rollers. Penicillium venetum was spotted onto the surface of the fruit or pericarps, as well as an aluminium plate to act as a control, before performing the plasma treatment. The results showed that viable cell number of P. venetum decreased with a decimal reduction time (D value or estimated treatment time required to reduce viable cell number by 90%) of 0.967 min on the aluminium plate, 2.90 min and 1.88 min on the pericarps of ‘Kiyomi’ (Citrus unshiu × C. sinensis) and ‘Kawano-natsudaidai’ (C. natsudaidai) respectively, and 2.42 min on the surface of ‘Unshu-mikan’ (C. unshiu). These findings confirmed a fungicidal effect of the plasma not only on an abiotic surface (aluminium plate) but also on a biotic surface (citrus fruit). Further development of the instrument by combining sorting systems with the plasma device promises an efficient means of disinfecting citrus fruits during food processing.

scholarly journals Nonpathogenic Isolates of the Citrus Black Spot Fungus, Guignardia citricarpa, Identified as a Cosmopolitan Endophyte of Woody Plants, G. mangiferae (Phyllosticta capitalensis)

2002 ◽  
Vol 92 (5) ◽  
pp. 464-477 ◽  
Author(s):  
R. P. Baayen ◽  
P. J. M. Bonants ◽  
G. Verkley ◽  
G. C. Carroll ◽  
H. A. van der Aa ◽  
...  
Keyword(s):  
United States ◽  
European Union ◽  
Woody Plants ◽  
Black Spot ◽  
The United States ◽  
The European Union ◽  
Citrus Fruits ◽  
Citrus Black Spot ◽  
Group I ◽  
Group Ii

The population structure of Guignardia citricarpa sensu lato (anamorph: Phyllosticta citricarpa), a fungus of which strains pathogenic to citrus are subject to phytosanitary legislation in the European Union and the United States, was investigated. Internal transcribed spacer sequences revealed two phylogenetically distinct groups in G. citricarpa. This distinction was supported by amplified fragment length polymorphism analysis that also supported the exclusion of two isolates that had apparently been misclassified as G. citricarpa. On cherry decoction agar, but not on other media, growth rates of group I isolates were lower than those of group II isolates. Conidial dimensions were similar, but group I isolates formed conidia with barely visible mucoid sheaths, whereas those of group II formed conidia with thick sheaths. Cultures of isolates belonging to group I produced rare infertile perithecia, whereas fertile perithecia were formed by most isolates of group II. Colonies of isolates belonging to group I were less dark than those of group II, with a wider translucent outer zone and a lobate rather than entire margin. On oatmeal agar, exclusively group I isolates formed a yellow pigment. Group I harbored strains from citrus fruits with classical black spot lesions (1 to 10 mm in diameter) usually containing pycnidia. Group II harbored endophytic strains from a wide range of host species, as well as strains from symptomless citrus fruits or fruits with minute spots (<2-mm diameter) without pycnidia. These observations support the historic distinction between slowly growing pathogenic isolates and morphologically similar fast-growing, nonpathogenic isolates of G. citricarpa. The latter proved to belong to G. mangiferae (P. capitalensis), a ubiquitous endophyte of woody plants with numerous probable synonyms including G. endophyllicola, G. psidii, P. anacardiacearum, and P. theacearum. G. mangiferae occurs in the European Union and the United States on many host species including citrus, and does not cause symptoms of citrus black spot, justifying its exclusion from quarantine measures.

scholarly journals CITRUS FRUIT QUALITY CLASSIFICATION BASED ON SIZE USING DIGITAL IMAGE PROCESSING

2019 ◽  
Vol 48 (5) ◽  
pp. 95-101
Author(s):  
Ulzii-Orshikh Dorj ◽  
Uranbaigal Dejidbal ◽  
Hongseok Chae ◽  
Lkhagvadorj Batsambuu ◽  
Altanchimeg Badarch ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Fruit Size ◽  
Citrus Fruit ◽  
Citrus Fruits ◽  
Single Tree ◽  
Quality Classification ◽  
Comparative Results ◽  
Vision Algorithm ◽  
Tree Fruits ◽  
Actual Size

A new computer vision algorithm for citrus fruit quality classification based on the size of a single tree fruits was developed in this study. The image properties of area, perimeter, and diameter for the citrus fruits were measured by pixels. In order to estimate citrus fruit size in a realistic manner, the ratios of diameter, perimeter and area in pixel values in relation to the actual size of one fruit were determined. The total of 1860 citrus fruits were grouped based on diameter, perimeter, and area in pixels. The results of the grouping of citrus fruits by diameter, perimeter and area were compared with the results of the survey research into citrus fruit size as conducted by the Jeju Citrus Commission. Comparative results reveal that the image of the citrus fruit diameter in pixels demonstrate a more accurate size than the other two pixel values, i.e. perimeter and area.

scholarly journals Hesperidin and SARS-CoV-2: New Light on the Healthy Functions of Citrus Fruit

2020 ◽  
Author(s):  
Paolo Bellavite ◽  
Alberto Donzelli
Keyword(s):  
Immune System ◽  
Citrus Sinensis ◽  
Viral Infections ◽  
Citrus Fruit ◽  
Short Review ◽  
Citrus Fruits ◽  
Early Proteins ◽  
Main Protease ◽  
The Many

Among the many approaches to COVID-19 prevention, the possible role of diet has so far been somewhat marginal. Nutrition is very rich in substances with a potential beneficial effect on health and some of these could have an antiviral action or in any case be important in modulating the immune system and in defending cells from the oxidative stress associated with infection. This short review draws the attention on some components of Citrus fruits and especially of the orange (Citrus sinensis), well known for its vitamin content, but less for the function of its flavonoids. Among the latter, hesperidin has recently attracted the attention of researchers, because it binds to the key proteins of the SARS-CoV-2 virus. Several computational methods, independently applied by different researchers, showed that hesperidin has a low binding energy both with the coronavirus "spike" protein, and with the main protease that transforms the early proteins of the virus (pp1a and ppa1b) into the complex responsible for viral replication. The affinity of hesperidin for these proteins is comparable if not superior to that of common chemical antivirals. The preventive efficacy of vitamin C, at dosage attainable by diet, against viral infections is controversial, but recent reviews suggest that this substance may be useful in case of increased stress on the immune system. Finally, the reasons that suggest undertaking appropriate research on the Citrus fruits addition in the diet, as a complementary prevention and treatment of COVID-19, are discussed.

Citrus bergamia, Risso: the peel, the juice and the seed oil of the bergamot fruit of Reggio Calabria (South Italy).

2020 ◽  
pp. 522
Author(s):  
Giuffrè Angelo Maria ◽  
Nobile Riccardo
Keyword(s):  
Seed Oil ◽  
Citrus Fruit ◽  
Total Flavonoids ◽  
Citrus Fruits ◽  
South Italy ◽  
The Past ◽  
Functional Aspects ◽  
Physico Chemical ◽  
Similar Quantity ◽  
Citrus Bergamia

Bergamot is a characteristic citrus fruit of the province of Reggio Calabria (South Italy) and until today it was mainly known for the production of its peel essential oil. Since some year, due to the positive feedback from consumers, there has been a growing interest in the bergamot juice which in the past was not used as a beverage because it was considered very sour. The recent studies on the functional aspects of citrus fruits and therefore also on bergamot have made this fruit known to a new youth. They exist three cultivars: Castagnaro, Fantastico and Femminello. In this study the biometrics of the fruits and the physico-chemical characteristics of the juice have been taken into consideration, such as: Brix degrees, pH, pulp content in the squeezed juice, turbidity, acidity, vitamin C, Formol number, single and total flavonoids. Total flavonoids in the bergamot juice ranged between 362 and 520 mg/L whereas in the Cloudy juice they ranged between 4660 and 8468 mg/L. The composition in fatty acids and in sterols of the seed oil of this fruit was also analysed to evaluate its potential. Oleic acid (30.15-34.36%) and linoleic acid (27.01-29.84%) were found in similar quantity, while the most present sterol was β-sitosterol (76.9-79.2%).

scholarly journals Review of Glutamate Intake from Both Food Additive and Non-Additive Sources in the European Union

2018 ◽  
Vol 73 (Suppl. 5) ◽  
pp. 21-28 ◽  
Author(s):  
David R. Tennant
Keyword(s):  
Glutamic Acid ◽  
Food Additives ◽  
Food Additive ◽  
Fermented Foods ◽  
Dietary Proteins ◽  
Natural Occurrence ◽  
The European Union ◽  
Small Children ◽  
High Level ◽  
Hydrolysis Of

Background: Intakes of glutamate can be derived from its natural occurrence as a constituent of proteins, from the presence of free glutamate in certain fermented foods, and from the addition of glutamic acid and glutamates to foods as flavor-enhancing additives. Summary: Intakes of glutamate following hydrolysis of dietary proteins can be as high as 440 mg/kg bw/day for toddlers and small children. High-level intakes of glutamate from its natural occurrence  in foods or from the use of food additives, given very conservative assumptions about conditions of use, are ­similar at around 80 mg/kg bw/day for toddlers and small children. Key Messages: The use of glutamic acid and glutamates as food additives makes a marginal contribution to total intakes of glutamate from all sources.

scholarly journals Hesperidin and SARS-CoV-2: New Light on the Healthy Function of Citrus Fruits

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 742
Author(s):  
Paolo Bellavite ◽  
Alberto Donzelli
Keyword(s):  
Immune System ◽  
Binding Energy ◽  
Computational Methods ◽  
Experimental Studies ◽  
Citrus Fruit ◽  
Short Review ◽  
Viral Disease ◽  
Antiviral Action ◽  
Citrus Fruits ◽  
The Many

Among the many approaches to Coronavirus disease 2019 (COVID-19) prevention, the possible role of nutrition has so far been rather underestimated. Foods are very rich in substances, with a potential beneficial effect on health, and some of these could have an antiviral action or be important in modulating the immune system and in defending cells from the oxidative stress associated with infection. This short review draws the attention on some components of citrus fruits, and especially of the orange (Citrus sinensis), well known for its vitamin and flavonoid content. Among the flavonoids, hesperidin has recently attracted the attention of researchers, because it binds to the key proteins of the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Several computational methods, independently applied by different researchers, showed that hesperidin has a low binding energy, both with the coronavirus “spike” protein, and with the main protease that transforms the early proteins of the virus (pp1a and ppa1b) into the complex responsible for viral replication. The binding energy of hesperidin to these important components is lower than that of lopinavir, ritonavir, and indinavir, suggesting that it could perform an effective antiviral action. Furthermore, both hesperidin and ascorbic acid counteract the cell damaging effects of the oxygen free radicals triggered by virus infection and inflammation. There is discussion about the preventive efficacy of vitamin C, at the dose achievable by the diet, but recent reviews suggest that this substance can be useful in the case of strong immune system burden caused by viral disease. Computational methods and laboratory studies support the need to undertake apposite preclinical, epidemiological, and experimental studies on the potential benefits of citrus fruit components for the prevention of infectious diseases, including COVID-19.

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