scholarly journals Biocompatibility of a HA/β-TCP/C Scaffold as a Pulp-Capping Agent for Vital Pulp Treatment: An In Vivo Study in Rat Molars

2021 ◽  
Vol 18 (8) ◽  
pp. 3936
Author(s):  
Julia Guerrero-Gironés ◽  
Antonia Alcaina-Lorente ◽  
Clara Ortiz-Ruiz ◽  
Eduardo Ortiz-Ruiz ◽  
María P. Pecci-Lloret ◽  
...  
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Biological Properties ◽  
Sprague Dawley ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Pulp Inflammation ◽  
Pulp Vitality ◽  
Rat Molars ◽  
Pulp Treatment

Bioceramic materials possess desirable biological properties, highlighting their non-reactivity and osteoconductivity. Their use has been extended in vital pulp treatment. The purpose of this study was to evaluate and compare the effects of beta-tricalcium phosphate (β-TCP), hydroxyapatite (HA), and collagen (C) scaffold with mineral trioxide aggregate (MTA) on the vital pulp of rat molars. Thirty-two molars of Sprague–Dawley rats underwent direct pulp capping with β-TCP/HA/C (n = 16) and MTA (n = 16). After 30 days, the following parameters were evaluated in the tested samples: the degree of pulp inflammation and pulp vitality, the presence of reparative dentin, the homogeneity of the odontoblastic layer, and the presence of pulp fibrosis. No statistically significant differences were observed between HA/β-TCP/C and MTA in terms of the degree of inflammation (p = 0.124). Significant differences were found in reparative dentin formation between the treatment groups (p = 0.0005). Dentin bridge formation was observed in the MTA-treated group. The local action of HA/β-TCP/C is similar to that of MTA when used as an agent for pulp vital treatment in terms of absence of inflammation and maintenance of pulp vitality, although there are significant differences between both materials regarding the formation of dentin bridges.

scholarly journals Melatonin as an Agent for Direct Pulp-Capping Treatment

2020 ◽  
Vol 17 (3) ◽  
pp. 1043 ◽  
Author(s):  
Julia Guerrero-Gironés ◽  
Antonia Alcaina-Lorente ◽  
Clara Ortiz-Ruiz ◽  
Eduardo Ortiz-Ruiz ◽  
María P. Pecci-Lloret ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dental Pulp ◽  
Bone Growth ◽  
Study Groups ◽  
Histological Evaluation ◽  
Sprague Dawley ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Pulp Inflammation

Melatonin plays an essential role in the regulation of bone growth. The actions that melatonin exerts on odontoblasts may be similar to its action on osteoblasts. This research aimed to evaluate the pulp response to melatonin used for direct pulp capping to evaluate the antioxidant effect of melatonin administered orally and its influence on dental pulp. Direct pulp capping was performed on the upper molars of Sprague Dawley rats using melatonin or Mineral Trioxide Aggregate (MTA). The study groups were: MTA; Melatonin; MTA + Melatonin administered orally; and Melatonin + Melatonin administered orally. In the latter two groups, the animals drank water dosed with melatonin ad libitum (10 mg/100 mL). After 30 days, the animals were sacrificed, and 5 ml of blood, the kidneys, and the liver were extracted in order to evaluate oxidative stress using thiobarbituric acid reactive substances testing (TBARS). Fragments of the maxilla containing the study molars were prepared for histological evaluation. The degree of pulp inflammation and pulp necrosis, the presence of reparative dentin and dentin bridging the pulp chamber, the presence and regularity of the odontoblastic layer, and the presence of pulp fibrosis were evaluated. No significant differences were found between the four study groups for any of the studied histological variables. The oral administration of melatonin did not modify the local effects of MTA or melatonin on dental pulp, or reduce basal-level oxidative stress. The effect of melatonin on pulp is similar to that of MTA and may be used as an agent for direct pulp capping.

scholarly journals Evaluation of clinical and radiological outcomes of mineral trioxide aggregate and calcium hydroxide as indirect pulp capping agents in the treatment of deep carious lesion of permanent teeth

2016 ◽  
Vol 9 (3) ◽  
pp. 140 ◽  
Author(s):  
Rafeza Sultana ◽  
Mozammal Hossain ◽  
Md. Shamsul Alam
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Permanent Teeth ◽  
Capping Agents ◽  
Pulp Capping ◽  
Carious Lesions ◽  
Reparative Dentin ◽  
Deep Carious Lesions ◽  
Pulp Vitality ◽  
Indirect Pulp Capping

<p>The maintenance of pulp vitality and conduction of reparative dentin can be possible by indirect pulp capping with mineral trioxide aggregate (MTA) and calcium hydroxide as pulp capping agents. The objective of the study is to assess the clinical and radiological outcomes of MTA and calcium hydroxide as indirect pulp capping agents in deep carious lesions of permanent teeth. The present study included 50 permanent teeth having deep carious lesions with reversible pulp status were selected and then randomly divided into two groups of 25 teeth in a group. Standard indirect pulp capping procedures were followed. Patients were recalled at 3, 6 and 12 months interval to assess postoperative pain, the vitality of the pulp and formation of reparative dentin. In all observation periods, MTA showed more capable of reducing pain and maintain pulp vitality which was statistically significant than that of calcium hydroxide. At 12 months observation period, 24 teeth (96%) of MTA and 19 teeth (76%) of calcium hydroxide showed reparative dentin formation. It can be concluded that MTA is more effective than that of calcium hydroxide.</p><p> </p>

scholarly journals Comparison of the biocompatibility of calcium silicate-based materials to mineral trioxide aggregate: Systematic review

2018 ◽  
Vol 12 (02) ◽  
pp. 317-326 ◽  
Author(s):  
Natália Gomes de Oliveira ◽  
Pollyana Rodrigues de Souza Araújo ◽  
Marina Torreão da Silveira ◽  
Ana Paula Veras Sobral ◽  
Marianne de Vasconcelos Carvalho
Keyword(s):  
Systematic Review ◽  
Web Of Science ◽  
Mineral Trioxide Aggregate ◽  
Biological Properties ◽  
Exclusion Criteria ◽  
Pulp Inflammation ◽  
Prisma Statement ◽  
Low Cytotoxicity

ABSTRACTThe objective of this systematic review was to evaluate the biocompatibility and interaction of bioceramic materials with animal and human mesenchymal cells in vitro and in vivo and to compare them with mineral trioxide aggregate (MTA). Two independent researchers conducted PubMed/Medline, Web of Science, and Scopus searches to identify studies published in English, without restrictions on year of publication using the following keywords: “root canal sealer,” “root repair material,” “cytotoxicity,” and “bioceramics.” The articles were selected following the PRISMA statement. A total of 1486 titles were identified in the initial search. However, only 18 studies met the inclusion and exclusion criteria. The results showed that bioceramic materials have biological properties similar to those of MTA, including low cytotoxicity as well as promoting cell proliferation and adhesion, low expression of inflammatory cytokines, and reduced pulp inflammation. This systematic review therefore suggests that the choice of repair bioceramic materials or MTA based on biocompatibility should be the professional's decision.

Decision-Making and Management of Immature Permanent Teeth with Crown Fractures in Small Animals—A Review

2021 ◽  
pp. 089875642110463
Author(s):  
Amalia Zacher ◽  
Sandra Manfra Marretta
Keyword(s):  
Treatment Options ◽  
Mineral Trioxide Aggregate ◽  
Permanent Teeth ◽  
Small Animals ◽  
Unique Challenge ◽  
Pulp Capping ◽  
Pulp Therapy ◽  
Vital Pulp Therapy ◽  
Pulp Vitality ◽  
And Function

Immature permanent teeth with crown fractures present a unique challenge in human and animal patients. Immature permanent teeth have not yet developed completely, often presenting with thin dentin walls, incomplete apical formation, and increased crown-to-root ratios. Loss of pulp function at this stage has devastating long-term implications for these teeth. Ideally, attempts should be made to preserve pulp vitality in immature permanent teeth to allow for continued dental development. The range of treatment options for vital teeth includes odontoplasty with bonding and sealing +/− restoration, indirect pulp capping, and direct pulp capping/vital pulp therapy. These treatments have long been established in human and veterinary medicine, and cases have been reported in dogs and cats. Apexification using calcium hydroxide is a well-established treatment for nonvital immature teeth. The advent of mineral trioxide aggregate and other bioceramic materials for use in vital pulp therapy and apexification has reduced treatment sessions and improved outcomes. Recent developments in the field of regenerative endodontic therapy further expand treatment options and provide the possibility for continued development of a formerly nonvital tooth. Selecting the appropriate treatment based on the severity of tooth fracture and status of pulp vitality can avoid a lifetime of poor structure and function for the affected tooth. This article provides multiple step-by-step protocols for the management of immature permanent teeth with crown fractures in small animals.

scholarly journals Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2102 ◽  
Author(s):  
Xuan Tran ◽  
Hamideh Salehi ◽  
Minh Truong ◽  
Minic Sandra ◽  
Jeremy Sadoine ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Calcium Silicate ◽  
Tissue Characterization ◽  
Mineral Trioxide Aggregate ◽  
Clinical Situation ◽  
Mineralized Tissue ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Inflammatory Status ◽  
Reparative Dentin

Nowadays, the preservation of dental pulp vitality is an integral part of our daily therapies. The success of these treatments depends on the clinical situation as well as the biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used as pulp capping materials. One objective of vital pulp therapy is the repair/regeneration of the pulp. In addition to the initial inflammatory status of the pulp, the nature and quality of the new mineralized tissue obtained after pulp capping directly influence the success of the treatment. In order to characterize the reparative dentin, in the current study, the chemical composition and microstructure of the dentin bridge after direct pulp capping using Biodentine™ and mineral trioxide aggregate (MTA) was studied by using Raman microspectroscopy and scanning electron microscopy, respectively. The results showed that the reparative dentin bridge observed in both groups presented dentin tubules and chemical composition similar to primary dentin. With the limitations of this study, the calcium-silicate-based cements used as pulp capping materials provide an optimal environment for pulp healing, resulting in a reparative dentin resembling on certain points of the primary dentin and the regeneration of the pulp.

scholarly journals Histomorphological response of dogs' dental pulp capped with white mineral trioxide aggregate

2004 ◽  
Vol 15 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Italo Medeiros Faraco Junior ◽  
Roberto Holland
Keyword(s):  
Dental Pulp ◽  
Healing Process ◽  
Mineral Trioxide Aggregate ◽  
Morphological Aspect ◽  
Bridge Formation ◽  
Pulp Capping ◽  
Exposure Site ◽  
Pulp Inflammation ◽  
White Mineral

This study was conducted to observe the response of dogs' dental pulp to white mineral trioxide aggregate (MTA) when used as pulp capping material. The pulp of 15 dogs' teeth was experimentally exposed and capped with white MTA. The animals were sacrificed two months later and the specimens were prepared for histomorphological study. The pulp capped with white MTA showed a healing process with complete dentin bridge formation in all samples. In some cases, there was not a tubular dentin shape, but only a structure with an interesting morphological aspect sealing the exposure site. Only 2 specimens exhibited pulp inflammation. In conclusion, the data obtained in this study showed that white MTA has the necessary properties of a pulp capping material.

scholarly journals Dental Pulp Defence and Repair Mechanisms in Dental Caries

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Jean-Christophe Farges ◽  
Brigitte Alliot-Licht ◽  
Emmanuelle Renard ◽  
Maxime Ducret ◽  
Alexis Gaudin ◽  
...  
Keyword(s):  
Dental Caries ◽  
Dental Pulp ◽  
Molecular Mechanisms ◽  
Inflammatory Responses ◽  
Oral Bacteria ◽  
Barrier Formation ◽  
Reparative Dentin ◽  
Pulp Inflammation

Dental caries is a chronic infectious disease resulting from the penetration of oral bacteria into the enamel and dentin. Microorganisms subsequently trigger inflammatory responses in the dental pulp. These events can lead to pulp healing if the infection is not too severe following the removal of diseased enamel and dentin tissues and clinical restoration of the tooth. However, chronic inflammation often persists in the pulp despite treatment, inducing permanent loss of normal tissue and reducing innate repair capacities. For complete tooth healing the formation of a reactionary/reparative dentin barrier to distance and protect the pulp from infectious agents and restorative materials is required. Clinical andin vitroexperimental data clearly indicate that dentin barrier formation only occurs when pulp inflammation and infection are minimised, thus enabling reestablishment of tissue homeostasis and health. Therefore, promoting the resolution of pulp inflammation may provide a valuable therapeutic opportunity to ensure the sustainability of dental treatments. This paper focusses on key cellular and molecular mechanisms involved in pulp responses to bacteria and in the pulpal transition between caries-induced inflammation and dentinogenic-based repair. We report, using selected examples, different strategies potentially used by odontoblasts and specialized immune cells to combat dentin-invading bacteriain vivo.

Sign in / Sign up

Export Citation Format

Share Document