scholarly journals Melatonin as an Agent for Direct Pulp-Capping Treatment

2020 ◽  
Vol 17 (3) ◽  
pp. 1043 ◽  
Author(s):  
Julia Guerrero-Gironés ◽  
Antonia Alcaina-Lorente ◽  
Clara Ortiz-Ruiz ◽  
Eduardo Ortiz-Ruiz ◽  
María P. Pecci-Lloret ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dental Pulp ◽  
Bone Growth ◽  
Study Groups ◽  
Histological Evaluation ◽  
Sprague Dawley ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Pulp Inflammation

Melatonin plays an essential role in the regulation of bone growth. The actions that melatonin exerts on odontoblasts may be similar to its action on osteoblasts. This research aimed to evaluate the pulp response to melatonin used for direct pulp capping to evaluate the antioxidant effect of melatonin administered orally and its influence on dental pulp. Direct pulp capping was performed on the upper molars of Sprague Dawley rats using melatonin or Mineral Trioxide Aggregate (MTA). The study groups were: MTA; Melatonin; MTA + Melatonin administered orally; and Melatonin + Melatonin administered orally. In the latter two groups, the animals drank water dosed with melatonin ad libitum (10 mg/100 mL). After 30 days, the animals were sacrificed, and 5 ml of blood, the kidneys, and the liver were extracted in order to evaluate oxidative stress using thiobarbituric acid reactive substances testing (TBARS). Fragments of the maxilla containing the study molars were prepared for histological evaluation. The degree of pulp inflammation and pulp necrosis, the presence of reparative dentin and dentin bridging the pulp chamber, the presence and regularity of the odontoblastic layer, and the presence of pulp fibrosis were evaluated. No significant differences were found between the four study groups for any of the studied histological variables. The oral administration of melatonin did not modify the local effects of MTA or melatonin on dental pulp, or reduce basal-level oxidative stress. The effect of melatonin on pulp is similar to that of MTA and may be used as an agent for direct pulp capping.

Preliminary Report on the Effects of Propolis on Wound Healing in the Dental Pulp

1998 ◽  
Vol 53 (11-12) ◽  
pp. 1045-1048 ◽  
Author(s):  
Walter A. Bretz ◽  
D. J. Chiego ◽  
M. C. Marcucci ◽  
I. Cunha ◽  
A. Custódio ◽  
...  
Keyword(s):  
Wound Healing ◽  
Calcium Hydroxide ◽  
Cell Population ◽  
Adult Male ◽  
Dental Pulp ◽  
Preliminary Report ◽  
Study Groups ◽  
Male Rats ◽  
Histological Evaluation ◽  
Reparative Dentin

Abstract The purpose of this investigation was to determine the antimicrobial and healing potential of propolis on direct dental pulp exposures. This study used 25 adult male rats. Pulp expo­sures were performed and animals were allocated to propolis and calcium hydroxide (Ca(OH)2 groups. Animals were killed on days 5, 7, 10, and 14. The teeth were routinely processed for histological evaluation. Non-parametric tests were employed to analyze the data. No significant differences were found between study groups on the wound healing of the dental pulp. Both substances were com parable in exhibiting normal reorganization of the pulp and no increased vascularity, and were equally efficacious in maintaining a low inflammatory and microbial cell population as well as in stimulating the formation of reparative dentin.

scholarly journals DEFENCE REACTION IN DENTAL PULP AFTER PULP CAPPING AND PARTIAL PULPECTOMY IN DOGS

2000 ◽  
Vol 48 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Z. Pavlica ◽  
M. Pogačnik ◽  
Polona Juntes
Keyword(s):  
Dental Pulp ◽  
Zinc Phosphate ◽  
Direct Pulp Capping ◽  
Defence Reaction ◽  
Pulp Capping ◽  
Single Root ◽  
Pulp Inflammation ◽  
Radical Method ◽  
Indirect Pulp Capping

The dental pulp was capped indirectly or directly, or partial vital pulpectomy was performed on the 12 functionally most important teeth of 24 beagle dogs. For pulp capping, calcium hydroxide was used, followed by zinc phosphate as a lining, and the preparation was restored with amalgam or composite material. Histological sections were prepared and examined for degree and type of pulp inflammation (hyperaemia, pulpitis, necrosis or gangrene). Degenerative changes of dental pulp (vacuolation, calcification, amyloid or hyaline changes) were also determined. For indirect pulp capping the width of predentine in crown and root dental pulp was measured, and for direct pulp capping and partial pulpectomy (vital pulpotomy) the width and quality of the dentinal bridge were graded. The radical method of partial vital pulpectomy of the coronal part of the dental pulp in dogs produced better quality and continuity of the dentinal bridge than the less radical method of direct pulp capping. This was particularly obvious in small single root teeth such as incisors.

Clinical and Histological Study on Direct Pulp Capping With CO2 Laser Irradiation in Human Teeth

2019 ◽  
Vol 44 (4) ◽  
pp. 336-347 ◽  
Author(s):  
M Suzuki ◽  
C Kato ◽  
S Kawashima ◽  
K Shinkai
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
Cooling System ◽  
Third Molar ◽  
Histological Evaluation ◽  
Air Cooling ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Laser Group

SUMMARYThe study aimed to histologically evaluate wound healing of exposed human pulp on direct pulp capping using super-pulsed CO2 laser preirradiation. In this single-blind clinical trial, 28 third molar teeth of 17 volunteers were randomly capped with either CO2 laser irradiation (n=14) or Dycal (calcium hydroxide cement; n=14) and restored using resin composite. The laser was operated in super-pulsed mode (pulse duration, 0.2 ms; interval, 5.8 ms; 0.003 J/pulse). The irradiation conditions were a power output of 0.5 W, an irradiation time of 15 seconds, repeat mode (10-ms irradiation and 10-ms intervals, for a total beam exposure time of 7.5 seconds), total applied energy of 3.75 J, and an activated air-cooling system. Each tooth was extracted at six or 12 months posttreatment and prepared for histological evaluation. We evaluated the parameters of pulp tissue disorganization, inflammatory cell infiltration, reparative dentin formation (RDF), and bacterial penetration. There were no significant differences between groups for all parameters at each postoperative period (Mann-Whitney U-test, p>0.05). CO2 laser irradiation completely controlled bleeding and exudate from the exposed pulp. The CO2 laser group had a tendency to delay RDF compared with the Dycal group, but 4 of 7 teeth from the CO2 laser group showed a complete dentin bridge at 12 months posttreatment.

scholarly journals Biocompatibility of a HA/β-TCP/C Scaffold as a Pulp-Capping Agent for Vital Pulp Treatment: An In Vivo Study in Rat Molars

2021 ◽  
Vol 18 (8) ◽  
pp. 3936
Author(s):  
Julia Guerrero-Gironés ◽  
Antonia Alcaina-Lorente ◽  
Clara Ortiz-Ruiz ◽  
Eduardo Ortiz-Ruiz ◽  
María P. Pecci-Lloret ◽  
...  
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Biological Properties ◽  
Sprague Dawley ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Pulp Inflammation ◽  
Pulp Vitality ◽  
Rat Molars ◽  
Pulp Treatment

Bioceramic materials possess desirable biological properties, highlighting their non-reactivity and osteoconductivity. Their use has been extended in vital pulp treatment. The purpose of this study was to evaluate and compare the effects of beta-tricalcium phosphate (β-TCP), hydroxyapatite (HA), and collagen (C) scaffold with mineral trioxide aggregate (MTA) on the vital pulp of rat molars. Thirty-two molars of Sprague–Dawley rats underwent direct pulp capping with β-TCP/HA/C (n = 16) and MTA (n = 16). After 30 days, the following parameters were evaluated in the tested samples: the degree of pulp inflammation and pulp vitality, the presence of reparative dentin, the homogeneity of the odontoblastic layer, and the presence of pulp fibrosis. No statistically significant differences were observed between HA/β-TCP/C and MTA in terms of the degree of inflammation (p = 0.124). Significant differences were found in reparative dentin formation between the treatment groups (p = 0.0005). Dentin bridge formation was observed in the MTA-treated group. The local action of HA/β-TCP/C is similar to that of MTA when used as an agent for pulp vital treatment in terms of absence of inflammation and maintenance of pulp vitality, although there are significant differences between both materials regarding the formation of dentin bridges.

scholarly journals Histological evaluation of human pulp capped with light-cured calcium based cements: a randomized controlled clinical trial

2017 ◽  
Vol 3 (5) ◽  
pp. 120 ◽  
Author(s):  
G. Jeya Gopika ◽  
Sathyanarayanan Ramarao ◽  
Carounanidy Usha ◽  
Bindu Meera John ◽  
N. Vezhavendhan
Keyword(s):  
Inflammatory Response ◽  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Permanent Teeth ◽  
Histological Evaluation ◽  
Controlled Clinical Trial ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Capping ◽  
Reparative Dentin

<p><strong>Background: </strong>Calcium hydroxide has traditionally been used as the pulp capping material for pulpal exposures in permanent teeth. The tunnel defects in the barrier and the tendency for dissolution, however, fails to provide permanent protection to the pulp. Light curable resin based cements have been introduced to enable a better marginal seal and lesser dissolution. The purpose of this study was to compare and evaluate the response of human pulp following direct pulp capping with the new resin based Calcium silicate (TheraCal LC) and Calcium hydroxide with hydroxyapatite (Septocal LC) cements compared with calcium hydroxide (Dycal). <strong></strong></p><p><strong>Methods: </strong>72 intact human premolars scheduled for orthodontic extractions were exposed to direct pulp capping procedures using three different pulp capping agents. Teeth were randomly divided into 3 groups, Group A: Dycal, Group B: TheraCal LC, Group C: Septocal LC. The teeth were extracted at the end of 15 and 40 days’ and were evaluated histologically. They were scored for reparative dentin formation and inflammatory response. Inferential statistics was done using Chi square test<strong>. </strong><strong></strong></p><p><strong>Results: </strong>Majority of the specimens in all three groups at the end of 15 days’ showed partial to lateral deposition of hard tissue. There was continuous deposition of hard tissue and severe inflammatory response at the end of 40 days’ in Dycal. There was partial deposition of hard tissue and reduced inflammatory response at the end of 40 days’ in TheraCal LC and Septocal LC. However, the results were not statistically significant between the three groups at two different time periods. <strong></strong></p><p><strong>Conclusions: </strong>Light cured,<strong> </strong>Calcium silicate (TheraCal LC) and Calcium hydroxide with hydroxyapatite (Septocal LC) cements were as effective as calcium hydroxide (Dycal) in inducing the formation of reparative dentin and evoking inflammatory response.</p>

Evaluation of Carbonate Apatite Cement in Inducing Formation of Reparative Dentin in Exposed Dental Pulp

2017 ◽  
Vol 758 ◽  
pp. 250-254 ◽  
Author(s):  
Myrna Nurlatifah Zakaria ◽  
Nisa Fitriani Nurul Pauziah ◽  
Indah Puti Sabirin ◽  
Arief Cahyanto
Keyword(s):  
Chronic Inflammation ◽  
Dental Pulp ◽  
Glass Ionomer Cement ◽  
In Vivo Studies ◽  
Histological Evaluation ◽  
Good Prospect ◽  
Carbonate Apatite ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Dental Application

Carbonate apatite (CO3Ap) cement is a biocompatible material with the ability to induce osteogenesis and is widely studied as the bone substitute material. The similarity of bone to tooth structure in their organic and inorganic composition offers a good prospect of the application of CO3Ap for regeneration of dentin and pulp tissue repair. This study is a sequel of the previous study in the development of CO3Ap cement for dental application, particularly in pulp capping treatment. In this study, the CO3Ap cement is used to cover an exposed dental pulp in rats and histological evaluation was carried out to evaluate the formation of reparative dentin, which is one of the signs of positive pulp capping treatment outcome. The CO3Ap cement was made by combining 60% dicalcium phosphate anhydrous (DCPA) and 40% vaterite with 1 mol/L Na2HPO4 aqueous solution at liquid to powder ratio of 0.5 and conventional calcium hydroxide [Ca(OH)2], which is the golden standard for pulp capping material was used for control. The coronal part of dental pulp in Wistar Rat was exposed with a small dental bur until bleeding were visible. The CO3Ap cement was applied to the exposed pulp and covered with light cured glass ionomer cement. The same protocol was applied to the control groups using Ca(OH)2. The Evaluation of formation of reparative dentin was done in 3 weeks after application by histological observation. Reparative dentin was histologically visible for all samples in CO3Ap group, as well as in the Ca(OH)2 groups. None of the dental pulps were necrotic and the pulp showed no heavy chronic inflammation. The CO3Ap cement was able to maintain the vitality of the pulp with no sign of chronic inflammation. The formation of reparative dentin was observable in 3 weeks of evaluation. Investigation on other properties and in vivo studies in different types of perforation and animal are to be carried out for further development of this material in dental application.

scholarly journals The thickness of odontoblast-like cell layer after induced by propolis extract and calcium hydroxide

2016 ◽  
Vol 49 (1) ◽  
pp. 17
Author(s):  
Irfan Dwiandhono ◽  
Ruslan Effendy ◽  
Sri Kunarti
Keyword(s):  
Calcium Hydroxide ◽  
Cell Layer ◽  
Tissue Response ◽  
Histological Evaluation ◽  
Bacterial Invasion ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Propolis Extract ◽  
Pulp Capping ◽  
Pulp Inflammation

Background: Propolis is a substance made from resin collected by bees (Apis mellifera) from variety of plants, mixed with its saliva and various enzymes to build a nest. Propolis has potential antimicrobial and antiinflammatory agents with some advantages over calcium hydroxide (Ca(OH)2). Ca(OH)2 has been considered as the “gold standard” of direct pulp-capping materials, but there are still some weakness of its application. First, it can induce pulp inflammation which last up to 3 months. Second, the tissue response to Ca(OH)2 is not always predictable. Third, the tunnel defect can probably formed in dentinal bridge with possible bacterial invasion in that gap. Purpose: This study was aimed to determine and compare the thickness of odontoblast-like cells layer after induced by propolis extract and Ca(OH)2 in rat’s pulp tissue. Method: Class 1 preparation was done in maxillary first molar tooth of wistar mice until the pulp opened. The Ca(OH)2 and propolis extract was applied to induce the formation of odontoblast-like cells, the cavity was filled with RMGIC. The teeth were extracted (after 14 and 28 days of induction). The samples were then processed for histological evaluation. Result: There were significant differences between the thickness of odontoblast-like cells after induced by propolis extract and Ca(OH)2. Conclusion: The propolis extract as the direct pulp capping agent produces thicker odontoblast-like cell layer compared to Ca(OH)2.

scholarly journals Direct Pulp Capping with a Dentin Bonding System in Human Teeth: A Clinical and Histological Evaluation

10.2341/05-65 ◽  
2006 ◽  
Vol 31 (3) ◽  
pp. 297-307 ◽  
Author(s):  
G. A. B. Silva ◽  
L. D. Lanza ◽  
N. Lopes-Júnior ◽  
A. Moreira ◽  
J. B. Alves
Keyword(s):  
Phosphoric Acid ◽  
Dental Pulp ◽  
Histological Evaluation ◽  
Synthetic Activity ◽  
Acid Etching ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Group I ◽  
Human Dental Pulp ◽  
Phosphoric Acid Etching

SUMMARY This study evaluated the pulpal response in human dental pulp to direct pulp capping with the Single Bond Adhesive System (SBAS) after 10% or 37% phosphoric acid etching and after capping with Calcium Hydroxide (CH). The degree of bleeding and hemostasis conditions was considered during the adhesive technique. The pulps of 78 sound premolars were capped with SBAS after 37% phosphoric acid etching (Group I) or 10% phosphoric acid etching (Group II) and CH (Group III-control). The cavities were restored with a resin composite (Charisma). After 1, 3, 7 and 30 days, the teeth were extracted and processed for light microscopical examination (H/E, AgNOR silver stain and Brown-Brenn). The patients were followed for postoperative symptomatology evaluation. Clinical results showed the possibility of hemostasis with saline solution only. There was no statistical difference between bleeding generated by 10% and 37% acid solutions. In some cases, contact of the pulp tissue with SASB started the bleeding process, thus damaging the adhesive technique. The histological response was similar in Groups I and II, without signs of cellular differentiation and dentin neoformation up to 30 days. Bacteria were not observed in any specimens. In the control group (CH) at day 7, the pulps exhibited cells with high synthetic activity (Ag-NOR-positive) underneath the area of coagulation necrosis. Dentin bridging was observed at the thirtieth day. The postoperative period was asymptomatic for all groups. In conclusion, SBAS should be avoided for vital pulp therapy, while CH remains the capping agent of choice for mechanically exposed human dental pulp.

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