Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries

Background. Severe early childhood caries (S-ECC) is a rapid form of dental caries that firstly affects primary upper incisors of children less <3 years of age and can cause interference in child’s health by pain, nutritional deficiencies and sleep disorders. It seems there are many unknown factors in the etiology as well as progression of S-ECC. The aim of this study was to assess parents' views in this relation. Methods. In this qualitative study parent’s views and their 1‒3-year-old children were studied when they visited pediatric medical clinics in Kerman. After cleaning the children teeth and examination of them to discover caries, they were placed in 2 groups with or without S-ECC. Then each parent was interviewed separately and their comments were collected and studied. Examinations and interviews continued with parents until they did not express anything new. Results. Parents of children without S-ECC had better understanding about S-ECC related factors than parents of children with S-ECC and greater number of them (without significant differences) expressed known reasons for occurrence of S-ECC. There were differences among known reasons and proposed reasons mostly in parents of children with S-ECC, although the differences were not significant. Maternal stress and amount of breast milk’s lactose were factors that were reported by some parents while there were unknown factors related to the etiology of S-ECC. Furthermore, no parents mentioned factors like saliva or mode of delivery. Conclusion. The most important achievement of the study was the attention of some parents to the role of their chronic stress in the occurrence of S-ECC. Another important consideration was that none of the parents mentioned the important role of the quality and quantity of saliva in preventing S-ECC, which should be promoted in the community.

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Magnetic Bead-Based Salivary Peptidome Profiling Analysis for Severe Early Childhood Caries

Caries Research ◽

10.1159/000360868 ◽

2014 ◽

Vol 49 (1) ◽

pp. 63-69 ◽

Cited By ~ 8

Author(s):

Yan Si ◽

Shuang Ao ◽

Weijian Wang ◽

Feng Chen ◽

Shuguo Zheng

Keyword(s):

Mass Spectrometry ◽

Early Childhood ◽

Early Childhood Caries ◽

Expression Profiles ◽

Salivary Protein ◽

Preschool Age ◽

Diagnostic Model ◽

Peptide Mass ◽

Severe Early Childhood Caries ◽

Childhood Caries

Objective: To investigate the differential salivary protein expression profiles between children with severe early childhood caries (S-ECC) and caries-free (CF) children at the age of 3 years. Methods: We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cation exchange magnetic beads, and peptide mass fingerprints were created by scanning mass spectrometry signals. Salivary samples from 20 children were analyzed (10 for each group). Results: Eleven protein peaks were significantly different (p < 0.05) between the two groups. Eight of these peaks were higher in the S-ECC group and three were higher in the CF group. To establish a diagnostic model for discrimination between the two groups, we chose three peptides (3,186.2, 3,195.8 and 3,324.8 Da) that exhibited the best fitted curve, by which the two groups were better separated when compared with other combinations. Conclusions: The salivary biomarkers identified revealed significant differences between the CF and the S-ECC group. Our results provide novel insight into the salivary protein profile of preschool-age children with dental caries and may lead to the development of a new strategy for screening high-risk populations.

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Dental Treatments under the General Anesthesia in a Child with Keratitis, Ichthyosis, and Deafness Syndrome

Case Reports in Dentistry ◽

10.1155/2013/618468 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6

Author(s):

Sera Sımsek Derelioglu ◽

Yücel Yılmaz ◽

Sultan Keles

Keyword(s):

Early Childhood ◽

Case Report ◽

General Anesthesia ◽

Early Childhood Caries ◽

Sensorineural Deafness ◽

Oral Symptoms ◽

Severe Early Childhood Caries ◽

Childhood Caries

KID syndrome is a rare genodermatosis characterized by keratitis, ichthyosis, and sensorineural deafness. Although the dermatological, ophthalmologic, and sensorineural defects are emphasized in the literature, oral and dental evaluations are so superficial. In this case report, dental and oral symptoms of a three year and five months old boy with KID syndrome, suffering severe Early Childhood Caries (s-ECC) and dental treatments done under General Anesthesia (GA) were reported.

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Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽

10.12688/f1000research.16275.1 ◽

2018 ◽

Vol 7 ◽

pp. 1645 ◽

Cited By ~ 7

Author(s):

Endang W. Bachtiar ◽

Boy M. Bachtiar

Keyword(s):

Early Childhood ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Dental Plaque ◽

Early Childhood Caries ◽

Positive Correlation ◽

Unstimulated Saliva ◽

Student’S T ◽

Total Bacteria ◽

Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

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