scholarly journals Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Comparative Proteome Analysis of the Response of Ramie under Drought Stress

2016 ◽  
Vol 17 (10) ◽  
pp. 1607 ◽  
Author(s):  
Xia An ◽  
Jingyu Zhang ◽  
Lunjin Dai ◽  
Gang Deng ◽  
Yiwen Liao ◽  
...  
Keyword(s):  
Drought Stress ◽  
Proteome Analysis ◽  
Absolute Quantitation ◽  
Isobaric Tags

scholarly journals Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency

2021 ◽  
Vol 43 (2) ◽  
pp. 1142-1155
Author(s):  
Zhi Nie ◽  
Bowen Luo ◽  
Xiao Zhang ◽  
Ling Wu ◽  
Dan Liu ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Proteome Analysis ◽  
Transcript Level ◽  
Utilization Efficiency ◽  
Differentially Expressed ◽  
Phosphate Deficiency ◽  
Absolute Quantitation ◽  
Protein Levels ◽  
Isobaric Tags

A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize.

Proteome Analysis of Testicular Tissue in Varicocele Rats Using iTRAQ Labeling Technology

2020 ◽  
Author(s):  
lu xian feng ◽  
zhao wei ◽  
liu jian rong ◽  
wang dan feng ◽  
yuan cai xia ◽  
...  
Keyword(s):  
Protein Transport ◽  
Proteome Analysis ◽  
Testicular Tissue ◽  
P Value ◽  
Absolute Quantitation ◽  
Tissue Samples ◽  
Itraq Analysis ◽  
Potential Biomarker ◽  
Liquid Chromatography Tandem Mass ◽  
Isobaric Tags

Abstract Purpose Varicocele (VC) is considered as the main cause of male infertility, clear and definite molecular markers of varicocele disease are helpful for early prevention and timely treatment. This study was in order to examine the effect of varicocele on protein expression in testicular tissue.Methods The testicular tissue samples of normal rats and varicocele rats were used for proteomic analysis and functional bioinformatics analysis. The proteins with a fold change of > 1.2 or < 1.2, and with a P-value < 0.05 were used to identify up- and downregulation proteins between the varicocele and control rats, and two target proteins were selected and verified with western blot.Results It was found that seminiferous epithelium was disordered and spermatogenic cells were injured seriously in varicocele group. A total of 65 differentially expressed proteins were identified compared with normal group by liquid chromatography tandem mass spectrometry (LC-MS/MS) and isobaric tags for relative and absolute quantitation (iTRAQ) analysis, including 31 up-regulated proteins and 34 down-regulated proteins, respectively. Functions of those proteins were mainly related to the following processes: signal transduction, protein cycle, transmembrane transport, protein transport, vesicular mediated transport, cell division, and membrane tissue. Two down regulated proteins of ATPase and Cu2+-transporting alpha (ATP7A) and Calcium and integrin-binding protein 1 (CIB1) in varicocele rats were selected and confirmed.Conclusion Protein spectrum of testicular tissue in varicocele rats was different from that in normal rats. Low expressions of ATP7A and CIB1 may be affect testicular spermatogenic function and can be used as a potential biomarker for testicular tissue to maintain normal spermatogenic function.

scholarly journals Isobaric tags for relative and absolute quantitation-based proteome analysis of Vietnamese colorectal carcinoma tissues

2018 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
ThiThu Hoai Nguyen ◽  
ThiThu Hien Pham ◽  
KimPhuong Uyen Le ◽  
PhuongUyen Vo ◽  
KieuMinh Le ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Proteome Analysis ◽  
Absolute Quantitation ◽  
Isobaric Tags

scholarly journals Translocation of Drought-Responsive Proteins from the Chloroplasts

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 259
Author(s):  
Ping Li ◽  
Haoju Liu ◽  
Hong Yang ◽  
Xiaojun Pu ◽  
Chuanhong Li ◽  
...  
Keyword(s):  
Drought Stress ◽  
Nicotiana Benthamiana ◽  
Physcomitrella Patens ◽  
Potential Importance ◽  
Absolute Quantitation ◽  
Chloroplast Proteins ◽  
Great Decline ◽  
Isobaric Tags ◽  
Retrograde Signal ◽  
Carbonic Anhydrase 1

Some chloroplast proteins are known to serve as messengers to transmit retrograde signals from chloroplasts to the nuclei in response to environmental stresses. However, whether particular chloroplast proteins respond to drought stress and serve as messengers for retrograde signal transduction are unclear. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) to monitor the proteomic changes in tobacco (Nicotiana benthamiana) treated with drought stress/re-watering. We identified 3936 and 1087 differentially accumulated total leaf and chloroplast proteins, respectively, which were grouped into 16 categories. Among these, one particular category of proteins, that includes carbonic anhydrase 1 (CA1), exhibited a great decline in chloroplasts, but a remarkable increase in leaves under drought stress. The subcellular localizations of CA1 proteins from moss (Physcomitrella patens), Arabidopsis thaliana and rice (Oryza sativa) in P. patens protoplasts consistently showed that CA1 proteins gradually diminished within chloroplasts but increasingly accumulated in the cytosol under osmotic stress treatment, suggesting that they could be translocated from chloroplasts to the cytosol and act as a signal messenger from the chloroplast. Our results thus highlight the potential importance of chloroplast proteins in retrograde signaling pathways and provide a set of candidate proteins for further research.

Differential Plant Proteome Analysis by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)

2013 ◽  
pp. 155-169 ◽  
Author(s):  
María J. Martínez-Esteso ◽  
Juan Casado-Vela ◽  
Susana Sellés-Marchart ◽  
María A. Pedreño ◽  
Roque Bru-Martínez
Keyword(s):  
Proteome Analysis ◽  
Absolute Quantitation ◽  
Isobaric Tags

A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology

2020 ◽  
Vol 23 (7) ◽  
pp. 649-657
Author(s):  
Dong-Jiang Liao ◽  
Xi-Ping Cheng ◽  
Nan Li ◽  
Kang-Li Liang ◽  
Hui Fan ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Kidney Tissue ◽  
Quantitative Information ◽  
Enzyme Linked Immunosorbent Assay ◽  
Absolute Quantitation ◽  
Western Blot Method ◽  
Close Relationship ◽  
Polymerase Chain ◽  
Isobaric Tags

Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission processes in LN have not been clarified but is of great need in the clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone. Methods: Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction) were used for verification. Results: We identified 136 proteins that marked quantitative information. Among them, Hp, Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations. Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx were screened out for a close relationship with the pathogenesis and development of LN, which is worth further study.

scholarly journals Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactyliferaL.)

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja ◽  
Wolfgang Rohde
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Date Palm ◽  
Light Harvesting ◽  
Binding Protein ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
Stress Conditions ◽  
Spectrometric Analysis ◽  
Salt And Drought Stress

This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar “Sagie” were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

scholarly journals Proteome Analysis of Date Palm (Phoenix dactylifera L.) under Severe Drought and Salt Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Date Palm ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
2D Electrophoresis ◽  
Severe Drought ◽  
Protein Abundance ◽  
Low Protein

Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

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