A New Approach for Glyco-Functionalization of Collagen-Based Biomaterials

The cell microenvironment plays a pivotal role in mediating cell adhesion, survival, and proliferation in physiological and pathological states. The relevance of extracellular matrix (ECM) proteins in cell fate control is an important issue to take into consideration for both tissue engineering and cell biology studies. The glycosylation of ECM proteins remains, however, largely unexplored. In order to investigate the physio-pathological effects of differential ECM glycosylation, the design of affordable chemoselective methods for ECM components glycosylation is desirable. We will describe a new chemoselective glycosylation approach exploitable in aqueous media and on non-protected substrates, allowing rapid access to glyco-functionalized biomaterials.

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Towards 4th generation biomaterials: a covalent hybrid polymer–ormoglass architecture

Nanoscale ◽

10.1039/c5nr04275e ◽

2015 ◽

Vol 7 (37) ◽

pp. 15349-15361 ◽

Cited By ~ 11

Author(s):

N. Sachot ◽

M. A. Mateos-Timoneda ◽

J. A. Planell ◽

A. H. Velders ◽

M. Lewandowska ◽

...

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Cell Fate ◽

Hybrid Materials ◽

Hybrid Polymer ◽

Fate Control ◽

Cell Fate Control

Nanostructured hybrid materials are an excellent option to create extracellular matrix-like environments for cell fate control in bone tissue engineering.

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The Effects of Ionising and Non-Ionising Electromagnetic Radiation on Extracellular Matrix Proteins

Cells ◽

10.3390/cells10113041 ◽

2021 ◽

Vol 10 (11) ◽

pp. 3041

Author(s):

Ren Jie Tuieng ◽

Sarah H. Cartmell ◽

Cliona C. Kirwan ◽

Michael J. Sherratt

Keyword(s):

Extracellular Matrix ◽

Electromagnetic Radiation ◽

Cell Biology ◽

Biological Effects ◽

Well Being ◽

Ecm Proteins ◽

Clinical Consequences ◽

Radiation Induced ◽

And Function ◽

Cellular Components

Exposure to sub-lethal doses of ionising and non-ionising electromagnetic radiation can impact human health and well-being as a consequence of, for example, the side effects of radiotherapy (therapeutic X-ray exposure) and accelerated skin ageing (chronic exposure to ultraviolet radiation: UVR). Whilst attention has focused primarily on the interaction of electromagnetic radiation with cells and cellular components, radiation-induced damage to long-lived extracellular matrix (ECM) proteins has the potential to profoundly affect tissue structure, composition and function. This review focuses on the current understanding of the biological effects of ionising and non-ionising radiation on the ECM of breast stroma and skin dermis, respectively. Although there is some experimental evidence for radiation-induced damage to ECM proteins, compared with the well-characterised impact of radiation exposure on cell biology, the structural, functional, and ultimately clinical consequences of ECM irradiation remain poorly defined.

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Non-cell-autonomous promotion of pluripotency induction mediated by YAP

10.1101/481127 ◽

2018 ◽

Author(s):

Amaleah Hartman ◽

Xiao Hu ◽

Xinyue Chen ◽

Anna E. Eastman ◽

Cindy Yang ◽

...

Keyword(s):

Cell Fate ◽

Pluripotent Stem Cells ◽

Cell Types ◽

Matricellular Proteins ◽

Fate Control ◽

Promotional Effect ◽

Cellular Context ◽

Opposing Effects ◽

Cell Fate Control ◽

Heterologous Cell

SUMMARYWhile Yes-associated protein (YAP) antagonizes pluripotency during early embryogenesis, it has also been shown to promote stemness of multiple stem cell types, including pluripotent stem cells. Whether cellular context underlies these distinct functions of YAP in pluripotency remains unclear. Here, we establish that depending on the specific cells in which it is expressed, YAP exhibits opposing effects on pluripotency induction from somatic cells. Specifically, YAP inhibits pluripotency induction cell-autonomously but promotes it non-cell-autonomously. For its non-cell-autonomous role, YAP alters the expression of many secreted and matricellular proteins including CYR61, which recapitulates the promotional effect when added as a recombinant protein. Thus, we define a unique YAP-driven non-cell-autonomous process that enhances pluripotency induction. Our work highlights the importance of considering the distinct contributions from heterologous cell types in deciphering the mechanism of cell fate control and calls for careful re-examination of the co-existing bystander cells in complex cultures or tissues.

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